Stereo and region-selective biosynthesis of two new dihydroartemisinic acid glycosides by suspension-cultured cells of Artemisia annua

Artemisinin has been used in the production of “artemisinin combination therapies” for the treatment of malaria. Feeding of precursors has been proven to be one of the most effective methods to enhance artemisinin production in plant cultured cells. At the current paper, the biosynthesis of artemisinin (ART) and its four analogs from dihydroartemisinic acid (DHAA) in suspension-cultured cells ofArtemisia annuawere investigated. ARTs were detected by HPLC/GC-MS and isolated by various chromatography methods. The structures of four DHAA metabolites, namely, dihydro-epi-deoxyarteannuin B, arteannuin I, arteannuin K, and 3-β-hydroxy-dihydro-epi-deoxyarteannuin B, were elucidated by physicochemical and spectroscopic analyses. The correlation between gene expression and ART content was investigated. The results of RT-PCR showed that DHAA could up-regulate expression of amorpha-4,11-diene synthase gene (ADS), amorpha-4,11-diene C-12 oxidase gene (CYP71AV1), and farnesyl diphosphate synthase gene (FPS) (3.19-, 7.21-, and 2.04-fold higher than those of control group, resp.), which indicated that biosynthesis processes from DHAA to ART were enzyme-mediated.

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Effects of dihydro-epi-deoxyarteannuin B on artemisinin biosynthesis, transcriptional profile and associated gene expression in suspension-cultured cells of Artemisia annua

Biochemical Engineering Journal ◽

10.1016/j.bej.2020.107633 ◽

2020 ◽

Vol 160 ◽

pp. 107633

Author(s):

Jianhua Zhu ◽

Chang Chen ◽

Liuyu Ding ◽

Lv Xu ◽

Yuanyuan Luo ◽

...

Keyword(s):

Gene Expression ◽

Cultured Cells ◽

Artemisia Annua ◽

Transcriptional Profile ◽

Suspension Cultured Cells ◽

Artemisinin Biosynthesis

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Induction of freezing tolerance in potato (Solanum commersonii) suspension cultured cells

Physiologia Plantarum ◽

10.1034/j.1399-3054.1992.840107.x ◽

1992 ◽

Vol 84 (1) ◽

pp. 41-48 ◽

Cited By ~ 1

Author(s):

Stephen P. Lee ◽

Baolong Zhu ◽

Tony H. H. Chen ◽

Paul H. Li

Keyword(s):

Freezing Tolerance ◽

Cultured Cells ◽

Solanum Commersonii ◽

Suspension Cultured Cells

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Seasonal and Post-harvest Accumulation of Artemisinin, Artemisinic Acid, and Dihydroartemisinic Acid in Three Accessions of Artemisia annua Cultivated in West Virginia, USA

Planta Medica ◽

10.1055/s-2008-1075155 ◽

2008 ◽

Vol 74 (03) ◽

Cited By ~ 6

Author(s):

JFS Ferreira

Keyword(s):

West Virginia ◽

Artemisia Annua ◽

Post Harvest ◽

Artemisinic Acid ◽

Dihydroartemisinic Acid

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Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

10.26686/wgtn.12597854.v1 ◽

2020 ◽

Author(s):

Ian Sims ◽

K Middleton ◽

AG Lane ◽

AJ Cairns ◽

A Bacic

Keyword(s):

Cultured Cells ◽

Oryza Sativa L ◽

Suspension Cultures ◽

Exchange Chromatography ◽

Phleum Pratense ◽

Arabinogalactan Protein ◽

Extracellular Polysaccharides ◽

Type Ii ◽

Hordeum Vulgare L ◽

Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

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Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

10.26686/wgtn.12597854 ◽

2020 ◽

Author(s):

Ian Sims ◽

K Middleton ◽

AG Lane ◽

AJ Cairns ◽

A Bacic

Keyword(s):

Cultured Cells ◽

Oryza Sativa L ◽

Suspension Cultures ◽

Exchange Chromatography ◽

Phleum Pratense ◽

Arabinogalactan Protein ◽

Extracellular Polysaccharides ◽

Type Ii ◽

Hordeum Vulgare L ◽

Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

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Transcriptional Response of Soybean Suspension-cultured Cells Induced by Nod Factors Obtained from Bradyrhizobium japonicum USDA110

Plant and Cell Physiology ◽

10.1093/pcp/pcf160 ◽

2002 ◽

Vol 43 (11) ◽

pp. 1314-1322

Author(s):

Tsuneo Hakoyama ◽

Tadashi Yokoyama ◽

Hiroshi Kouchi ◽

Ken-ichi Tsuchiya ◽

Hisatoshi Kaku ◽

...

Keyword(s):

Bradyrhizobium Japonicum ◽

Cultured Cells ◽

Transcriptional Response ◽

Nod Factors ◽

Suspension Cultured Cells

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Phytotropins. IV. Effect of Phytotropins on Cultured Plant Cells and Protoplasts

Functional Plant Biology ◽

10.1071/pp9820297 ◽

1982 ◽

Vol 9 (3) ◽

pp. 297 ◽

Cited By ~ 6

Author(s):

PJ Larkin ◽

W Scowcroft ◽

AE Geissler ◽

GF Katekar

Keyword(s):

Petunia Hybrida ◽

Cultured Cells ◽

Plant Cells ◽

Dichlorophenoxyacetic Acid ◽

Polar Transport ◽

Suspension Cultured Cells ◽

Specific Receptors ◽

Nicotiana Debneyi ◽

2,4 Dichlorophenoxyacetic Acid

The phytotropins l-(2'-carboxyphenyl)-3-phenylpropane-1,3-dione (CPP) and 5-(2-carboxyphenyl)- 3-phenylpyrazole (CPD) reduced the net efflux of radiolabel from suspension-cultured cells treated with [14C]2,4-dichlorophenoxyacetic acid when present at concentrations comparable to those that inhibit polar transport of auxins in bean petioles. These phytotropins stimulated division of protoplasts of both Nicotiana debneyi and Petunia hybrida at concentrations of exogenous auxins that were otherwise suboptimal for divisions. The results are consistent with the proposal that phytotropins interact with specific receptors to reduce auxin efflux, resulting in increased intracellular auxin concentrations.

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