scholarly journals Isolation and two-step classification of normal white blood cells in peripheral blood smears

2012 ◽  
Vol 3 (1) ◽  
pp. 13 ◽  
Author(s):  
Nisha Ramesh ◽  
MohammedE Salama ◽  
Bryan Dangott ◽  
Tolga Tasdizen
2021 ◽  
pp. jclinpath-2021-207863
Author(s):  
Lisa N van der Vorm ◽  
Henriët A Hendriks ◽  
Simone M Smits

AimsRecently, a new automated digital cell imaging analyser (Sysmex CellaVision DC-1), intended for use in low-volume and small satellite laboratories, has become available. The purpose of this study was to compare the performance of the DC-1 with the Sysmex DI-60 system and the gold standard, manual microscopy.MethodsWhite blood cell (WBC) differential counts in 100 normal and 100 abnormal peripheral blood smears were compared between the DC-1, the DI-60 and manual microscopy to establish accuracy, within-run imprecision, clinical sensitivity and specificity. Moreover, the agreement between precharacterisation and postcharacterisation of red blood cell (RBC) morphological abnormalities was determined for the DC-1.ResultsWBC preclassification and postclassification results of the DC-1 showed good correlation compared with DI-60 results and manual microscopy. In addition, the within-run SD of the DC-1 was below 1 for all five major WBC classes, indicating good reproducibility. Clinical sensitivity and specificity were, respectively, 96.7%/95.9% compared with the DI-60% and 96.6%/95.3% compared with manual microscopy. The overall agreement on RBC morphology between the precharacterisation and postcharacterisation results ranged from 49% (poikilocytosis) to 100% (hypochromasia, microcytosis and macrocytosis).ConclusionsThe DC-1 has proven to be an accurate digital cell imaging system for differential counting and morphological classification of WBCs and RBCs in peripheral blood smears. It is a compact and easily operated instrument that can offer low-volume and small satellite laboratories the possibilities of readily available blood cell analysis that can be stored and retrieved for consultation with remote locations.


2017 ◽  
Vol 5 (5) ◽  
pp. 221-231
Author(s):  
Muhamed Katica ◽  
Nedzad Gradascevic

The laboratory rat, as important biomedical model, was often fed with unconventional diet usually made up of products from the bakery industry. Such diet consisted of insufficient caloric and nutritionally unbalanced meals could cause unreliable results in biomedical research. The study investigates the effects of malnutrition on the haematological profile of rats. The study is performed on Wistar male and female rats which were fed for 4 weeks exclusively with bakery products ad libidum. The following hematological parameters were observed in peripheral blood smears: red blood cell count, content of haemoglobin, haematocrit, MCV, MCH, MCHC, white blood cell count, differential blood count, diameter of red blood cells, as well as the presence of atypical forms of red blood cells. Despite there were no statistically significant differences in overall haematological results (p > 0.05, with > 0.05), the significant part of obtained results were below physiological limits (HGB, MCHC and MCH). Other haematological parameters, including white blood corpuscles were kept in physiological limits, except for mild neutrophils in males. Also, the forms of anulocytes and spherocytes were recorded in peripheral blood smears. The results indicated the beginning of normocytic hypochromic anaemia which was caused by unbalanced meals.


2018 ◽  
Vol 11 (6) ◽  
pp. 7-13
Author(s):  
Ali Mohammad Alqudah ◽  
◽  
Ola Al-Ta’ani ◽  
Alaa Al-Badarneh ◽  
◽  
...  

1987 ◽  
Vol 11 (5) ◽  
pp. 475-480 ◽  
Author(s):  
John F. Vago ◽  
Paul E. Hurtubise ◽  
Orlando J. Martelo ◽  
Steven H. Swerdlow

2019 ◽  
Vol 12 (1) ◽  
pp. 18-21
Author(s):  
Archana Shrivatava ◽  
◽  
Upasana Uniya ◽  
Reeni Malik ◽  
◽  
...  

2018 ◽  
Vol 11 (6) ◽  
pp. 7-13
Author(s):  
Ali Mohammad Alqudah ◽  
◽  
Ola Al-Ta’ani ◽  
Alaa Al-Badarneh ◽  
◽  
...  

2014 ◽  
Vol 37 (5) ◽  
Author(s):  
Ines Gütgemann ◽  
Hermann Heimpel ◽  
Carl Thomas Nebe

AbstractTeardrop erythrocytes (syn. dakryocytes) play a key role in the evaluation of peripheral blood smears in patients with anemia, especially as part of the “leukoerythroblastic picture”. Teardrop-shaped red blood cells can be seen in a wide range of diseases that lead to bone marrow fibrosis, which is often accompanied by extramedullary hematopoesis. The differential diagnoses encompass primary myelofibrosis (PMF), myelodysplastisc syndromes during the late course of the disease, rare forms of acute leucemias and myelophtisis caused by metastatic carcinoma. Rarely, MF associated with post-irradiation, toxins, autoimmune diseases, metabolic conditions, inborn hemolytic anemias, iron-deficient anemia or β-thalassaemia can lead to the formation of teardrops as visualized on peripheral blood smears.


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