scholarly journals Microscopy versus enzyme linked immunosorbent assay test for detection of Entamoeba histolytica infection in stool samples

2014 ◽  
Vol 4 (2) ◽  
pp. 136 ◽  
Author(s):  
Srujana Mohanty ◽  
Nisha Sharma ◽  
Monorama Deb
Keyword(s):  
Entamoeba Histolytica ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Histolytica Infection ◽  
Stool Samples

scholarly journals High occurrence of Entamoeba histolytica in the municipalities of Ariquemes and Monte Negro, State of Rondônia, Western Amazonia, Brazil

2013 ◽  
Vol 55 (3) ◽  
pp. 193-196 ◽  
Author(s):  
Rafael Vital dos Santos ◽  
Jucélia da Silva Nunes ◽  
Juliana Almeida de Souza Aranha Camargo ◽  
Eliana Maria Maurício da Rocha ◽  
Gilberto Fontes ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Odds Ratio ◽  
Detection Rate ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Occurrence ◽  
Significant Difference ◽  
Histolytica Infection ◽  
Stool Antigen ◽  
Stool Samples ◽  
Two Populations

Introduction: Entamoeba histolytica infections were investigated in residents of the Ariquemes and Monte Negro municipalities in Rondônia State, Brazil. Methods: Stool samples of 216 individuals were processed by the spontaneous sedimentation method and analyzed by microscopy for detection of the E. histolytica/E. dispar complex, followed by the immunoassay method using an enzyme-linked immunosorbent assay-based kit for the E. histolytica stool antigen. Results: E. histolytica/E. dispar cysts were present in 61% (50/82) and 44% (59/134) of the samples from Ariquemes and Monte Negro respectively, with a significant difference in the occurrence of infection between the two populations [p < 0.05; χ2 = 5.2; odds ratio = 2.0 (1.1 - 3.6)]. The E. histolytica antigen detection rate was 36.6% (30/82) for stool samples from Ariquemes, and 19.4% (26/134) for stool taken from the residents of Monte Negro. The rate of the occurrence of amoebiasis was significantly higher in the population from Ariquemes [p < 0.05; χ2 = 7.8; odds ratio = 2.4 (1.2 - 4.7)]. Discussion: Due to the high occurrence of E. histolytica infected residents diagnosed in the region and the unavailability in local clinics of a test to distinguish between the two Entamoeba species, physicians should consider treating E. histolytica/E.dispar infections. Conclusion: The results indicate that E. histolytica infection is highly endemic in the studied areas.

scholarly journals Detection of Entamoeba histolytica Coproantigen Among Children with Dysentery in Ahvaz, Southwest Iran

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Ahmad Shamsizadeh ◽  
Roya Nikfar ◽  
Mahmoud Rahdar
Keyword(s):  
Entamoeba Histolytica ◽  
False Positive ◽  
Correct Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Medical Laboratories ◽  
Negative Results ◽  
Histolytica Infection ◽  
Stool Samples ◽  
Southwest Iran ◽  
Diagnosis Of Infection

: Entamoeba histolytica is one of the important parasitic diseases in many parts of the world, especially the tropical and subtropical regions. The parasite is transmitted through contaminated water and vegetables. The exact diagnosis of infection with the parasite is crucial in many medical laboratories since there are many false positive and negative results in their reports. Therefore, the current study aimed at evaluating and comparing microscopic and coproantigen ELISA (the enzyme-linked immunosorbent assay) results to reach an appropriate test for the correct diagnosis of amoebiasis in children. One hundred stool samples were collected from children under 15 years old with dysenteric diarrhea from April to September 2018. Microscopic tests and coproantigen ELISA were performed on all the samples. The results showed that 5% of the samples had E. histolytica/E. dispar cysts. The findings of ELISA to detect coproantigen did not show any specific E. histolytica antigen in the samples. Hence, all the patients received chemotherapy for shigellosis. E. histolytica infection is not the main causative agent for dysenteric diarrhea in children in the studied area, and laboratory experts should be trained to prevent false-positive reports.

scholarly journals A Comparative Analysis of Microscopy, Coproantigen Serology, and Nested Multiplex PCR in the Laboratory Diagnosis of Entamoeba histolytica Infection

2021 ◽  
Author(s):  
Sindhusuta Das ◽  
Nonika Rajkumari ◽  
Anitha Gunalan ◽  
Dhanalakshmi Rajavelu ◽  
Jeby Jose Olickal
Keyword(s):  
Multiplex Pcr ◽  
Entamoeba Histolytica ◽  
Predictive Value ◽  
Parasitic Infection ◽  
Laboratory Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Kappa Statistics ◽  
Histolytica Infection ◽  
Intestinal Amoebiasis ◽  
Stool Samples

Abstract Objectives Amoebiasis is caused by the most common intestinal protozoan parasite Entamoeba histolytica. This parasite causes amoebic colitis, which is manifested by diarrhea, followed by dysentery. The laboratory diagnosis of intestinal amoebiasis in most cases is by microscopic examination of stool samples. Other nonroutine methods include coproantigen enzyme-linked immunosorbent assay (ELISA) from stool samples, serum ELISA for antibodies, stool culture, isoenzyme analysis, and polymerase chain reaction (PCR). The present study aimed to comparatively analyze the different diagnostic modalities used for the detection of E. histolytica from the stool sample of patients with intestinal amoebiasis. Materials and Methods This study was undertaken with 631 patients, during a period of 3 years, from January 2017 to December 2019. Stool specimen obtained from each patient was subjected to direct microscopic wet mount examination, coproantigen ELISA, and nested multiplex PCR, respectively. Results Out of all the patients tested, 5.2% were positive for E. histolytica. Among the positive cases, stool microscopy was positive in 3.17%, coproantigen ELISA was positive in 29 (4.6%) cases, and PCR was positive in 30 (4.75%) cases. Statistical Analysis The prevalence of E. histolytica infection was summarized as percentages. The three diagnostic tests done were statistically analyzed, taking microscopy as the gold standard. The agreement between techniques (microscopy, coproantigen ELISA, and PCR) was analyzed with kappa statistics. Sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy were summarized as percentage with 95% confidence interval. Conclusion In all suspected amoebiasis cases, a combination of stool microscopy, coproantigen testing with molecular detection of the parasite offers the best approach to diagnosis of this parasitic infection.

scholarly journals The NF-κB p50 Subunit Is Protective during Intestinal Entamoeba histolytica Infection of 129 and C57BL/6 Mice

2010 ◽  
Vol 78 (4) ◽  
pp. 1475-1481 ◽  
Author(s):  
Kyou-Nam Cho ◽  
Stephen M. Becker ◽  
Eric R. Houpt
Keyword(s):  
Entamoeba Histolytica ◽  
Electrical Resistance ◽  
Transepithelial Electrical Resistance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mobility Shift ◽  
Protective Function ◽  
Targeted Deletion ◽  
Nuclear Extracts ◽  
Histolytica Infection ◽  
Mobility Shift Assay

ABSTRACT Entamoeba histolytica is the agent of amebic colitis. In this work we examined the intestinal NF-κB response to this parasite. Using an enzyme-linked immunosorbent assay (ELISA) and an electrophoretic mobility shift assay, we found that the NF-κB subunit p50 predominated in nuclear extracts of whole cecal tissue and of isolated crypts from mice inoculated with E. histolytica. p50 was protective, since C57BL/6 and 129 mice in which there was targeted deletion of this subunit were more susceptible to E. histolytica infection as measured by culture results, cecal parasite ELISA results, and/or histologic scores. The transepithelial electrical resistance of cecal explants from C57BL/6 and 129 p50 knockout mice decreased markedly in response to the parasite compared with the transepithelial electrical resistance of their wild-type counterparts, suggesting that a protective function of p50 was present in the epithelium itself. This work shows that NF-κB activity, particularly activity of the p50 subunit, is one factor that contributes to resistance of the gut to E. histolytica infection.

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