scholarly journals Tracking of Actinobacillus actinomycetemcomitans in subgingival plaque of aggressive periodontitis patients

2010 ◽  
Vol 2 (2) ◽  
pp. 64 ◽  
Author(s):  
Supriya Kheur ◽  
Atya Kapley ◽  
VinayK Hazarey ◽  
Hemant Purohit
Keyword(s):  
Aggressive Periodontitis ◽  
Actinobacillus Actinomycetemcomitans ◽  
Subgingival Plaque

scholarly journals Occurrence of Actinobacillus actinomycetemcomitans in patients with chronic periodontitis, aggressive periodontitis, healthy subjects and children with gingivitis in two cities of the state of São Paulo, Brazil

2006 ◽  
Vol 14 (3) ◽  
pp. 153-156 ◽  
Author(s):  
Elerson Gaetti Jardim Júnior ◽  
Joseane Maria Dias Bosco ◽  
Angélica Marquezim Lopes ◽  
Luís Fernando Landucci ◽  
Ellen Cristina Gaetti Jardim ◽  
...  
Keyword(s):  
School Children ◽  
Chronic Periodontitis ◽  
Healthy Subjects ◽  
Aggressive Periodontitis ◽  
Actinobacillus Actinomycetemcomitans ◽  
Subgingival Plaque ◽  
Facultative Anaerobe ◽  
Microbial Identification ◽  
Two Cities ◽  
Rate Of Recovery

The aim of this study was to determine the frequency of isolation of Actinobacillus actinomycetemcomitans (Aa) in 100 patients with chronic periodontitis, 14 patients with aggressive periodontitis, 142 pre-school children with gingivitis and 134 periodontally healthy subjects. Samples of subgingival plaque were taken using sterilized paper points introduced into periodontal pockets or gingival crevice for 60 seconds and inoculated on TSBV agar, which was incubated under anaerobiosis at 37ºC, for 4 days. Microbial identification was performed through biochemical methods and morphocellular and morphocolonial analysis. Aa was detected in 40.3% of healthy subjects, 68% of patients with chronic periodontitis, 92.86% of patients with aggressive periodontitis and 40.14% of children with gingivitis. The rate of recovery of Aa in the tested human groups proved to be higher than previously reported and in agreement with participation of this facultative anaerobe as a member of native microbiota of the periodontium and its relation with aggressive and chronic periodontitis in Brazil.

scholarly journals Genome Sequence of Aggregatibacter actinomycetemcomitans Serotype c Strain D11S-1

2009 ◽  
Vol 191 (23) ◽  
pp. 7378-7379 ◽  
Author(s):  
Casey Chen ◽  
Weerayuth Kittichotirat ◽  
Yan Si ◽  
Roger Bumgarner
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Aggressive Periodontitis ◽  
Etiological Agent ◽  
Subgingival Plaque

ABSTRACT Aggregatibacter actinomycetemcomitans is a major etiological agent of periodontitis. Here we report the complete genome sequence of serotype c strain D11S-1, which was recovered from the subgingival plaque of a patient diagnosed with generalized aggressive periodontitis.

Concurrence between the gene expression pattern of Actinobacillus actinomycetemcomitans in localized aggressive periodontitis and in human epithelial cells

2005 ◽  
Vol 54 (5) ◽  
pp. 497-504 ◽  
Author(s):  
Joseph Richardson ◽  
Justin Corey Craighead ◽  
Sam Linsen Cao ◽  
Martin Handfield
Keyword(s):  
Gene Expression ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Expression Pattern ◽  
Gene Expression Pattern ◽  
Aggressive Periodontitis ◽  
Actinobacillus Actinomycetemcomitans ◽  
Bacterial Gene ◽  
Bacterial Gene Expression

Actinobacillus actinomycetemcomitans is a facultatively intracellular pathogen and the aetiological agent of localized aggressive periodontitis. Screening of the genome of A. actinomycetemcomitans for in vivo-induced antigen determinants previously demonstrated that the proteome of this organism differs in laboratory culture compared with conditions found during active infection. The aim of the present study was to determine whether the bacterial gene expression pattern inferred with in vivo-induced antigen technology (IVIAT) in human infections was consistent with the gene expression pattern occurring upon epithelial cell association. To this end, a real-time PCR method was developed and used to quantify absolute and relative bacterial gene expression of A. actinomycetemcomitans grown extra- and intracellularly in two human epithelial cell lines (HeLa and IHGK). The amount of template used in the assay was normalized using the total count of viable bacteria (c.f.u.) as a reference point and performed in duplicate in at least two independent experiments. Controls for this experiment included 16S rRNA and gapdh. Transcription of all eight ORFs tested increased significantly (P < 0.05) in HeLa and IHGK cells compared with bacteria grown extracellularly. The concurrence of gene expression patterns found in the two models suggests that these epithelial cells are valid in vitro models of infection for the genes tested. IVIAT is an experimental platform that can be used as a validation tool to assess the reliability of animal and other models of infection and is applicable to most pathogens.

Identification of Actinobacillus actinomycetemcomitans in subgingival plaque by PCR.

1995 ◽  
Vol 33 (12) ◽  
pp. 3102-3105 ◽  
Author(s):  
T F Flemmig ◽  
S Rüdiger ◽  
U Hofmann ◽  
H Schmidt ◽  
B Plaschke ◽  
...  
Keyword(s):  
Actinobacillus Actinomycetemcomitans ◽  
Subgingival Plaque

scholarly journals Detection of highly and minimally leukotoxic Actinobacillus actinomycetemcomitans strains in patients with periodontal disease

2003 ◽  
Vol 17 (2) ◽  
pp. 183-188 ◽  
Author(s):  
Sheila Cavalca Cortelli ◽  
Antonio Olavo Cardoso Jorge ◽  
José Roberto Cortelli ◽  
Shawn Francis Jordan ◽  
Violet Ibyola Haraszthy
Keyword(s):  
Periodontal Disease ◽  
Strong Association ◽  
Aggressive Periodontitis ◽  
Actinobacillus Actinomycetemcomitans ◽  
Periodontal Pocket ◽  
Chain Reaction ◽  
Chi Square ◽  
Pocket Depth ◽  
Polymerase Chain ◽  
Brazilian Cohort

This study examined the prevalence of highly and minimally leukotoxic Actinobacillus actinomycetemcomitans in patients with periodontal disease. Pooled subgingival plaque samples from 136 patients with some form of periodontal disease were examined. Subjects were between 14 and 76 years of age. Clinical examinations included periodontal pocket depth (PD), plaque index (PI) and bleeding index (BI). The obtained plaque samples were examined for the presence of highly or minimally leukotoxic A. actinomycetemcomitans strains by the polymerase chain reaction (PCR). Chi-square and logistic regression were performed to evaluate the results. Forty-seven subjects were diagnosed with gingivitis, 70 with chronic periodontitis and 19 with aggressive periodontitis. According to chi-square there was no significant correlation detected between PD (chi2 = 0.73), PI (chi2 = 0.35), BI (chi2 = 0.09) and the presence of the highly leukotoxic A. actinomycetemcomitans. The highly leukotoxic A. actinomycetemcomitans strains were correlated with subjects that were 28 years of age and younger (chi2 = 7.41). There was a significant correlation between highly leukotoxic A. actinomycetemcomitans and aggressive periodontitis (chi2 = 22.06). This study of a Brazilian cohort confirms the strong association between highly leukotoxic A. actinomycetemcomitans strains and the presence of aggressive periodontitis.

Intragenomic recombination in the highly leukotoxic JP2 clone of Actinobacillus actinomycetemcomitans

Microbiology ◽  
2005 ◽  
Vol 151 (10) ◽  
pp. 3371-3379 ◽  
Author(s):  
Kirsten T. Eriksen ◽  
Dorte Haubek ◽  
Knud Poulsen
Keyword(s):  
Homologous Recombination ◽  
Dna Fingerprinting ◽  
Restriction Enzyme ◽  
Multilocus Sequence Typing ◽  
African Descent ◽  
Rflp Analysis ◽  
Aggressive Periodontitis ◽  
Genome Rearrangements ◽  
Actinobacillus Actinomycetemcomitans

The highly leukotoxic JP2 clone of Actinobacillus actinomycetemcomitans is strongly associated with aggressive periodontitis in adolescents of African descent. DNA fingerprinting using the frequently cutting restriction enzyme MspI and multilocus sequence typing (MLST) showed that five strains of this clone were genetically virtually identical, although ribotyping of the six rrn genes and EcoRI RFLP analysis of the seven IS150-like elements revealed differences. PCR analyses demonstrated that these multi-copy sequences are subject to intragenomic homologous recombination, resulting in translocations or large inversions. The genome rearrangements were reflected in differences among 25 strains representing the JP2 clone in DNA fingerprinting using the rare-cutting restriction enzyme XhoI and resolved by PFGE. XhoI DNA fingerprinting provides a tool for studying local epidemiology, including transmission of this particularly pathogenic clone of A. actinomycetemcomitans.

scholarly journals Putative periodontal pathogens in the subgingival plaque of Sudanese subjects with aggressive periodontitis

2017 ◽  
Vol 81 ◽  
pp. 97-102 ◽  
Author(s):  
N.T. Hashim ◽  
G.J. Linden ◽  
L. Winning ◽  
M.E. Ibrahim ◽  
B.G. Gismalla ◽  
...  
Keyword(s):  
Aggressive Periodontitis ◽  
Subgingival Plaque ◽  
Periodontal Pathogens
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