Evaluation of antiangiogenic potential of Psidium guajava leaves using In-Ovo chick chorioallantoic membrane assay

2018 ◽  
Vol 14 (57) ◽  
pp. 284
Author(s):  
Rajani Mathur ◽  
S Latha ◽  
P Yamini
Keyword(s):  
Chorioallantoic Membrane ◽  
Psidium Guajava ◽  
In Ovo ◽  
Chick Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay

scholarly journals Chick Chorioallantoic Membrane Assay: A 3D Animal Model for Study of Human Nasopharyngeal Carcinoma

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0130935 ◽  
Author(s):  
Xue Xiao ◽  
Xiaoying Zhou ◽  
Huixin Ming ◽  
Jinyan Zhang ◽  
Guangwu Huang ◽  
...  
Keyword(s):  
Animal Model ◽  
Nasopharyngeal Carcinoma ◽  
Chorioallantoic Membrane ◽  
Chick Chorioallantoic Membrane ◽  
Human Nasopharyngeal Carcinoma ◽  
Chorioallantoic Membrane Assay

scholarly journals Effect of miR-146a-5p on tumor growth in NSCLC using chick chorioallantoic membrane assay and bioinformatics investigation

2017 ◽  
Vol 16 (6) ◽  
pp. 8781-8792 ◽  
Author(s):  
Wen-Ting Huang ◽  
Wei-Luan Cen ◽  
Rong-Quan He ◽  
You Xie ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Chorioallantoic Membrane ◽  
Chick Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay

scholarly journals Evolving applications of the egg: chorioallantoic membrane assay and ex vivo organotypic culture of materials for bone tissue engineering

2020 ◽  
Vol 11 ◽  
pp. 204173142094273
Author(s):  
Karen M Marshall ◽  
Janos M Kanczler ◽  
Richard OC Oreffo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Organotypic Culture ◽  
Ex Vivo ◽  
Chorioallantoic Membrane ◽  
Experimental Methodology ◽  
Chick Chorioallantoic Membrane ◽  
Recent Developments ◽  
Chorioallantoic Membrane Assay

The chick chorioallantoic membrane model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the chorioallantoic membrane assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the chorioallantoic membrane assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches, including timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo chorioallantoic membrane assay and the ex vivo organotypic culture to advance the field of bone tissue engineering, and highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

scholarly journals Functional involvement of carbonic anhydrase in calcium transport of the chick chorioallantoic membrane

1978 ◽  
Vol 176 (1) ◽  
pp. 67-74 ◽  
Author(s):  
R S Tuan ◽  
J Zrike
Keyword(s):  
Carbonic Anhydrase ◽  
Chorioallantoic Membrane ◽  
Carbonic Anhydrase Activity ◽  
Calcium Deposition ◽  
Dependent Manner ◽  
Transport Activity ◽  
In Ovo ◽  
Chick Chorioallantoic Membrane ◽  
Age Dependent

Carbonic anhydrase activity was demonstrated in the chick-embryonic chorioallantoic membrane and was correlated with the Ca2+-transport activity of the membrane. It is inhibited by sulphonamides and is expressed in the chorioallantoic membrane in an age-dependent fashion during embryonic development. Ca2+ uptake by the chorioallantoic membrane in vivo also increases in a similar age-dependent manner. The temporal increase in these activities is coincident with calcium deposition in the embryonic skeleton. Incubation of the chorioallantoic membrane in ovo with sulphonamides specifically inhibits both the carbonic anhydrase and the Ca2+ uptake activities of the membrane in vivo. Enzyme histochemistry revealed the carbonic anhydrase activity is localized in the Ca2+-transporting ectodermal cells of the chorioallantoic membrane. These results, taken together, indicate that carbonic anhydrase may be functionally important in the Ca2+-transport activity of the chorioallantoic membrane.

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