scholarly journals IGF-I Exerts an Anti-inflammatory Effect on Skeletal Muscle Cells through Down-regulation of TLR4 Signaling

2011 ◽  
Vol 11 (4) ◽  
pp. 223 ◽  
Author(s):  
Won Jun Lee
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Down Regulation ◽  
Tlr4 Signaling ◽  
Igf I ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Proteome Analysis of the Anti-inflammatory Response of Flavonoids Isolated from Korean Citrus aurantium L. in Lipopolysaccharide-Induced L6 Rat Skeletal Muscle Cells

2013 ◽  
Vol 41 (04) ◽  
pp. 901-912 ◽  
Author(s):  
Jin-A Kim ◽  
Hyeon-Soo Park ◽  
Kwang-Il Park ◽  
Gyeong-Eun Hong ◽  
Arulkumar Nagappan ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Inflammatory Response ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Citrus Aurantium ◽  
Maldi Tof Ms ◽  
L6 Cells ◽  
Anti Inflammatory ◽  
Tof Ms ◽  
Inflammatory Effect

Skeletal muscle is an important organ in our body and a dynamic composite of proteins. Citrus aurantium L. has been widely used in oriental medicine in Eastern Asia for a long time. It contains over 100 bioactive compounds and flavonoids that regulate the inflammatory response and tumorigenesis, through various mechanisms. In the present study, we investigated changes in the protein pattern using two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization time of flight mass spectroscopy (MALDI-TOF/MS) to assess the anti-inflammatory effect of flavonoids isolated from Korean C. aurantium L. in lipopolysaccharide (LPS)-induced L6 cells. L6 skeletal muscle cells were pretreated with flavonoids for 1 h and stimulated with LPS for 24 h. Proteins from the L6 cells of the control, LPS treated and flavonoid treated groups were extracted and resolved by 2-DE using pH 4–7 IPG strips loaded with 150 μg of protein. Forty-one differentially expressed protein spots were identified (more than two-fold was considered significant, p < 0.05), and 18 were detected by MALDI-TOF/MS. These results suggest that proteomics can be used to identify changes in the expression of marker proteins and the anti-inflammatory effect of flavonoids isolated from Korean C. aurantium L.

Anti-inflammatory Effect Of Avenanthramides Via Nf-κB Pathways In C2c12 Skeletal Muscle Cells.

2016 ◽  
Vol 48 ◽  
pp. 583 ◽  
Author(s):  
Choung-Hun Kang ◽  
Woo Shik Shin ◽  
Dongwook Yeo ◽  
Wonchung Lim ◽  
Li Li Ji
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Anti Inflammatory ◽  
C2c12 Skeletal Muscle Cells ◽  
Inflammatory Effect

Anti-inflammatory effect of avenanthramides via NF-κB pathways in C2C12 skeletal muscle cells

2018 ◽  
Vol 117 ◽  
pp. 30-36 ◽  
Author(s):  
Chounghun Kang ◽  
Woo Shik Shin ◽  
Dongwook Yeo ◽  
Wonchung Lim ◽  
Tianou Zhang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Anti Inflammatory ◽  
C2c12 Skeletal Muscle Cells ◽  
Inflammatory Effect

scholarly journals Isolation and validation of human prepubertal skeletal muscle cells: maturation and metabolic effects of IGF-I, IGFBP-3 and TNFα

2005 ◽  
Vol 568 (1) ◽  
pp. 229-242 ◽  
Author(s):  
Malcolm Grohmann ◽  
Emily Foulstone ◽  
Gavin Welsh ◽  
Jeff Holly ◽  
Julian Shield ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Metabolic Effects ◽  
Igf I ◽  
Igfbp 3

scholarly journals Focal adhesion kinase is required for IGF-I-mediated growth of skeletal muscle cells via a TSC2/mTOR/S6K1-associated pathway

2013 ◽  
Vol 305 (2) ◽  
pp. E183-E193 ◽  
Author(s):  
Hannah Crossland ◽  
Abid A. Kazi ◽  
Charles H. Lang ◽  
James A. Timmons ◽  
Philippe Pierre ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Growth ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Muscle Hypertrophy ◽  
Kinase Activity ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Cell Phenotype ◽  
Igf I

Focal adhesion kinase (FAK) is an attachment complex protein associated with the regulation of muscle mass through as-of-yet unclear mechanisms. We tested whether FAK is functionally important for muscle hypertrophy, with the hypothesis that FAK knockdown (FAK-KD) would impede cell growth associated with a trophic stimulus. C2C12 skeletal muscle cells harboring FAK-targeted (FAK-KD) or scrambled (SCR) shRNA were created using lentiviral transfection techniques. Both FAK-KD and SCR myotubes were incubated for 24 h with IGF-I (10 ng/ml), and additional SCR cells (±IGF-1) were incubated with a FAK kinase inhibitor before assay of cell growth. Muscle protein synthesis (MPS) and putative FAK signaling mechanisms (immunoblotting and coimmunoprecipitation) were assessed. IGF-I-induced increases in myotube width (+41 ± 7% vs. non-IGF-I-treated) and total protein (+44 ± 6%) were, after 24 h, attenuated in FAK-KD cells, whereas MPS was suppressed in FAK-KD vs. SCR after 4 h. These blunted responses were associated with attenuated IGF-I-induced FAK Tyr397 phosphorylation and markedly suppressed phosphorylation of tuberous sclerosis complex 2 (TSC2) and critical downstream mTOR signaling (ribosomal S6 kinase, eIF4F assembly) in FAK shRNA cells (all P < 0.05 vs. IGF-I-treated SCR cells). However, binding of FAK to TSC2 or its phosphatase Shp-2 was not affected by IGF-I or cell phenotype. Finally, FAK-KD-mediated suppression of cell growth was recapitulated by direct inhibition of FAK kinase activity in SCR cells. We conclude that FAK is required for IGF-I-induced muscle hypertrophy, signaling through a TSC2/mTOR/S6K1-dependent pathway via means requiring the kinase activity of FAK but not altered FAK-TSC2 or FAK-Shp-2 binding.

IGF-I and vitamin C promote myogenic differentiation of mouse and human skeletal muscle cells at low temperatures

2011 ◽  
Vol 317 (3) ◽  
pp. 356-366 ◽  
Author(s):  
Ai Shima ◽  
Jennifer Pham ◽  
Erica Blanco ◽  
Elisabeth R. Barton ◽  
H. Lee Sweeney ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Vitamin C ◽  
Low Temperatures ◽  
Human Skeletal Muscle ◽  
Myogenic Differentiation ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells ◽  
Igf I

scholarly journals GH Regulation of IGF-I and Suppressor of Cytokine Signaling Gene Expression in C2C12 Skeletal Muscle Cells

Endocrinology ◽  
2001 ◽  
Vol 142 (9) ◽  
pp. 3890-3900 ◽  
Author(s):  
Cynthia L. Sadowski ◽  
Thomas T. Wheeler ◽  
Lu-Hai Wang ◽  
Henry B. Sadowski
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Cytokine Signaling ◽  
Suppressor Of Cytokine Signaling ◽  
Igf I ◽  
C2c12 Skeletal Muscle Cells

scholarly journals Anti-inflammatory activities of sinensetin on LPS-stimulated L6 skeletal muscle cells

2019 ◽  
Vol 20 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Jin-A Kim ◽  
Sang Eun Ha ◽  
Seong Min Kim ◽  
Preethi Vetrivel ◽  
Gon Sup Kim ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Anti Inflammatory ◽  
L6 Skeletal Muscle Cells
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