Microvessel and Lymphatic Vessel Density and VEGFR-3 Expression of Papillary Thyroid Carcinoma with Comparative Analysis of Clinicopathological Characteristics

The CHEK2 gene is involved in the repair of damaged DNA. CHEK2 germline mutations impair this repair mechanism, causing genomic instability and increasing the risk of various cancers, including papillary thyroid carcinoma (PTC). Here, we asked whether CHEK2 germline mutations predict a worse clinical course for PTC. The study included 1547 unselected PTC patients (1358 women and 189 men) treated at a single center. The relationship between mutation status and clinicopathological characteristics, treatment responses, and disease outcome was assessed. CHEK2 mutations were found in 240 (15.5%) of patients. A CHEK2 I157T missense mutation was found in 12.3%, and CHEK2 truncating mutations (IVS2 + 1G > A, del5395, 1100delC) were found in 2.8%. The truncating mutations were more common in women (p = 0.038), and were associated with vascular invasion (OR, 6.91; p < 0.0001) and intermediate or high initial risk (OR, 1.92; p = 0.0481) in multivariate analysis. No significant differences in these parameters were observed in patients with the I157T missense mutation. In conclusion, the CHEK2 truncating mutations were associated with vascular invasion and with intermediate and high initial risk of recurrence/persistence. Neither the truncating nor the missense mutations were associated with worse primary treatment response and outcome of the disease.

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Diagnostic Value of Immunohistochemistry for the Detection of the BRAFV600E Mutation in Papillary Thyroid Carcinoma: Comparative Analysis with Three DNA-Based Assays

Thyroid ◽

10.1089/thy.2013.0302 ◽

2014 ◽

Vol 24 (5) ◽

pp. 858-866 ◽

Cited By ~ 44

Author(s):

Marius I. Ilie ◽

Sandra Lassalle ◽

Elodie Long-Mira ◽

Christelle Bonnetaud ◽

Olivier Bordone ◽

...

Keyword(s):

Comparative Analysis ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Diagnostic Value ◽

Papillary Thyroid ◽

Brafv600e Mutation

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Intratumoural lymph vessel density is related to presence of lymph node metastases and separates encapsulated from infiltrative papillary thyroid carcinoma

Virchows Archiv ◽

10.1007/s00428-011-1161-3 ◽

2011 ◽

Vol 459 (6) ◽

pp. 595-605 ◽

Cited By ~ 16

Author(s):

C. Eloy ◽

J. Santos ◽

P. Soares ◽

M. Sobrinho-Simões

Keyword(s):

Lymph Node ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Lymph Node Metastases ◽

Vessel Density ◽

Lymph Vessel ◽

Papillary Thyroid ◽

Node Metastases ◽

Lymph Vessel Density

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Clinicopathological Significance of Loss of p27Kip1 Expression in Papillary Thyroid Carcinoma

The International Journal of Biological Markers ◽

10.5301/jbm.5000239 ◽

2017 ◽

Vol 32 (2) ◽

pp. 255-259 ◽

Cited By ~ 4

Author(s):

Nae Yu Kim ◽

Jin Hwa Kim ◽

Jung-Soo Pyo ◽

Won Jin Cho

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Meta Analysis ◽

Thyroid Tissue ◽

Clinicopathological Characteristics ◽

Papillary Thyroid ◽

Benign Lesions ◽

Thyroid Microcarcinoma ◽

Clinicopathological Significance ◽

Expression Loss

Introduction A meta-analysis was done to investigate the clinicopathological significance of the loss of p27kip1 expression in papillary thyroid carcinoma (PTC). Methods The meta-analysis involving 17 studies included 1,652 PTC and 328 benign cases. The rate of p27kip1 expression loss in PTC and benign lesions, and the correlations between p27kip1 expression loss and clinicopathological characteristics of PTC were determined. Results The estimated rate of p27kip1 expression loss was 0.557 (95% confidence interval [CI] 0.443-0.665) and 0.139 (95% CI 0.062-0.283) in PTC and benign lesions, respectively. In subgroup analysis, the rates of p27kip1 expression loss were 0.683, 0.393, and 0.414 in the classical variant, follicular variant, and papillary thyroid microcarcinoma, respectively. Loss of p27kip1 expression was significantly correlated with lymph node metastasis and distant metastasis (odds ratio 3.559, 95% CI 1.146-11.056 and 4.735, 95% CI 1.322-16.960, respectively). Extrathyroidal extension was correlated with loss of p27kip1 expression, but not in a statistically significant way (p = 0.051). There were no significant correlations between loss of p27kip1 expression and sex, tumor size, BRAFV600E mutation, and tumor multifocality. Conclusions Loss of p27kip1 expression is frequently found in PTC compared with benign lesions and normal thyroid tissue. When present in PTC, it is correlated with aggressive tumor behavior.

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Comparative analysis of therapeutic efficacy of 131I in different clinical stages postoperative patients with papillary thyroid carcinoma

The Chinese-German Journal of Clinical Oncology ◽

10.1007/s10330-012-1003-z ◽

2012 ◽

Vol 11 (7) ◽

pp. 399-402

Author(s):

Li Zhao ◽

Shuyao Zuo ◽

Guoming Wang

Keyword(s):

Comparative Analysis ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Therapeutic Efficacy ◽

Papillary Thyroid ◽

Clinical Stages

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Conventional Papillary Thyroid Carcinoma With Intralobular Lymphatic Dissemination Shows More Aggressive Features

10.21203/rs.3.rs-91174/v1 ◽

2020 ◽

Author(s):

Yuanyuan Lei ◽

Wenting Huang ◽

Qiuxiao Yu ◽

Sha Feng ◽

Guihua Shen ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Vascular Tumor ◽

Biological Indicator ◽

Clinicopathological Characteristics ◽

Clinicopathological Features ◽

Braf V600e ◽

Tumor Diameter ◽

Papillary Thyroid ◽

Significant Difference

Abstract Objective: To investigate the invasive capability and other clinicopathological features of conventional papillary thyroid carcinoma (CVPTC) with intralobular lymphatic dissemination.Methods: Seventy-three CVPTC patients receiving total thyroidectomy were analyzed in this study. The expression of BRAF-V600E, D2-40 and CD31 in all thyroid samples was detected by immunohistochemical staining (IHC). The results were evaluated by two pathologists and were statistically analyzed. In addition, the rate of positive BRAF-V600E expression and the clinical invasiveness of CVPTC with intralobular dissemination (ID-CVPTC), multiple primary CVPTC (MP-CVPTC) and single focus CVPTC (SF-CVPTC) were evaluated. The correlation between BRAF-V600E expression, lymphatic vessel density (LVD), microvessel density (MVD) and the clinicopathological characteristics of CVPTC were assessed.Results: Twenty-five ID-CVPTC, 17 MP-CVPTC and 31 SF-CVPTC cases were included in this study. The positive expression rate of BRAF-V600E in ID-CVPTC (92.0%) was significantly higher than that in MP-CVPTC (70.6%) and SF-CVPTC (71.0%), while no significant difference in expression between MP-CVPTC and SF-CVPTC was detected (P > 0.05). The expression of BRAF-V600E was not related to clinicopathological features, including age, gender, lymph node metastasis (LNM), bilateral involvement, presence of vascular tumor thrombus, capsule invasion, nerve invasion or the maximum tumor diameter (P > 0.05). The LVD in the ID-CVPTC group (9.74 ± 2.98) was higher than that in the non-ID-CVPTC group (7.46 ± 2.5) (P < 0.05). Compared with cases without adenolobar dissemination, ID-CVPTC was associated with a younger age, higher LNM rate, and increased capsule and vessel invasiveness (P < 0.05).Conclusions: ID-CVPTC shows more aggressive features, and intralobular lymphatic dissemination may be a potential biological indicator of poor prognosis.

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BRAF V600E Immunoexpression in Papillary Thyroid Carcinoma and Its Association with Prognostic Factors and Histopathologic Variant

Medicinus ◽

10.19166/med.v8i1.3120 ◽

2021 ◽

Vol 8 (1) ◽

pp. 12

Author(s):

Erna Kristiani ◽

Endang SR Hardjolukito ◽

Agnes S Harahap ◽

Benyamin Makes

Keyword(s):

Prognostic Factors ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Clinicopathological Characteristics ◽

Braf V600e Mutation ◽

Braf V600e ◽

Papillary Thyroid ◽

Tall Cell Variant ◽

Tall Cell ◽

Cell Variant

Aim: to provide additional information regarding the clinicopathological characteristics of Papillary Thyroid Carcinoma (PTC). Methods: Fifty patient with PTC were reviewed to determine prognostic factors such as age, gender, size of tumor and histologic variant. BRAF V600E mutation was detected by immunohistochemical staining and assessed with H score. Result: BRAF V600E mutations were detected in 17 (34%) cases. There were seven cases with extrathyroidal extension (ETE) p 0,04, 11 cases with lymph node metastasis (LNM) p < 0,001, and 8 cases with tall cell variant p 0,047.The cases with positive BRAF V600E mutation had mean age of 44.71 years, and the size of the tumor between 0.1-4cm. Six cases of them are male and 11 female.Conclusion: There were significant relationships between BRAF V600E mutation with ETE, LNM, and tall cell variant. There was no significant relationship between BRAF V600E mutation, either with age, gender, or size of the tumor. BRAF V600E immunohistochemical examination can be performed as additional investigation for PTC patients.

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