scholarly journals Evaluation of the cytotoxic and genotoxic effect of Allium cepa L. (Amaryllidaceae) root cells after exposure in water samples of five lakes of Alta Floresta, State of Mato Grosso

2020 ◽  
Vol 15 (1) ◽  
pp. 1 ◽  
Author(s):  
Leila Pereira Neves Ramos ◽  
Douglas Machado Leite ◽  
Weslaine De Almeida Macedo ◽  
Cyntia Beatriz Magalhães Farias ◽  
Ademilso Sampaio de Oliveira ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Water Samples ◽  
Water Volume ◽  
Cytotoxic Activities ◽  
Cytotoxic Potential ◽  
Root Cells ◽  
Mato Grosso ◽  
Allium Cepa L ◽  
Cell Cycle Inhibition ◽  
Positive Controls

  This study evaluated genotoxic and cytotoxic potential of water samples through the analysis of Allium cepa root cells exposed to samples from five urban lakes of Alta Floresta, state of Mato Grosso. The samples were obtained in the dry and rainy periods, October 2014 and April 2015, respectively, at five distinct points. The collection points were as follows: Lake 01, located in MT 206; Lake 02, in Avenida Perimetral Rogério Silva; Lake 03, in Sector C; Lake 04 on Teles Pires Avenue; and Lake 05, in the Cidade Bela District. Bioassays using Allium cepa bulbs were taken from the water at each point, and comparisons were made with the negative and positive controls. The results found the presence of genotoxic and cytotoxic activities on the roots of A. cepa, indicating high potential in cell cycle inhibition. This result may also be caused by the influences of seasonal periods, taking into account that in the rainy season there is a greater transport of cytotoxic substances by rainwater, and in the dry season, a higher concentration of pollutants due to the reduction of water volume, resulting in a greater interaction of the concentrators as well as a greater concentration of the elements found in the water of these lagoons.

Aluminium induced oxidative stress and DNA damage in root cells of Allium cepa L.

2008 ◽  
Vol 70 (2) ◽  
pp. 300-310 ◽  
Author(s):  
V. Mohan Murali Achary ◽  
Suprava Jena ◽  
Kamal K. Panda ◽  
Brahma B. Panda
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Allium Cepa ◽  
Root Cells ◽  
Allium Cepa L

Comparative evaluation of promutagens o-PDA, m-PDA and MH for genotoxic response in root cells of Allium cepa L.

The Nucleus ◽  
2010 ◽  
Vol 53 (1-2) ◽  
pp. 45-50 ◽  
Author(s):  
Manosij Ghosh ◽  
Jit Paul ◽  
Sonali Sinha ◽  
Anita Mukherjee
Keyword(s):  
Allium Cepa ◽  
Comparative Evaluation ◽  
Root Cells ◽  
Allium Cepa L

scholarly journals POTENCIAL CITOTÓXICO E GENOTÓXICO DA ÁGUA DE RIOS DO MUNICÍPIO DE CARLINDA, MATO GROSSO, BRASIL, POR MEIO DO TESTE Allium cepa L.

2015 ◽  
Vol 2 (3) ◽  
pp. 71-80
Author(s):  
Kelli Zortéa ◽  
Jociele Lemes ◽  
Maiara Nascimento ◽  
Ana Paula Tafarelo ◽  
Ana Rossi
Keyword(s):  
Allium Cepa ◽  
Mato Grosso ◽  
Allium Cepa L

scholarly journals Antiproliferative action of aqueous extracts of Hymenaea stigonocarpa Mart. (Fabaceae) on the cell cycle of Allium cepa L.

2014 ◽  
Vol 86 (3) ◽  
pp. 1147-1150 ◽  
Author(s):  
LOURRAN P. LACERDA ◽  
GEIZ MALAQUIAS ◽  
ANA PAULA PERON
Keyword(s):  
Cell Cycle ◽  
Allium Cepa ◽  
Test System ◽  
Control Group ◽  
Aqueous Extracts ◽  
Root Cells ◽  
Antiproliferative Action ◽  
Allium Cepa L ◽  
Meristematic Root ◽  
Number Of Cells

In this study we evaluated the action of crude aqueous extracts obtained from rhytidome of Hymenaea stigonocarpa (jatobá-do-cerrado) on Allium cepa meristematic root cells in three concentrations: 0.082, 0.164, 0.328g/mL, at exposure times of 24 and 48 h. The slides were prepared by the crushing technique, and cells analyzed throughout the cell cycle, totaling 5000 for each control group and concentration. It was found that all three concentrations, including the lowest which is considered ideal for use, in all exposure times, had significant antiproliferative action on the cell cycle of this test system. For cells under division, we observed a high number of cells in prophase. Therefore, under the conditions studied H. stigonocarpa indicated to be cytotoxic.

scholarly journals Cytological changes in meristematic cells of Allium cepa L. root tip treated with extracts from callus of Catharanthus roseus (L.) G. Don

2014 ◽  
Vol 68 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Agnieszka Pietrosiuk ◽  
Mirosława Furmanowa ◽  
Alicja Zobel ◽  
Mieczysław Kuraś ◽  
Agnieszka Michalak
Keyword(s):  
Allium Cepa ◽  
Catharanthus Roseus ◽  
Ethanolic Extract ◽  
Root Tip ◽  
Root Cells ◽  
Irregular Structures ◽  
Allium Cepa L ◽  
Green Callus ◽  
Pharmacologically Active ◽  
Tip Cells

The effect of an ethanolic extract from callus of <em>Catharanthus roseus</em> on <em>Allium cepa</em> root cells divisions was investigated. Two lines: white and green callus, were established on solid B5 medium with IAA 1 mg/l and kinetin 0.1 mg/l. The HPLC analysis of callus extracts showed the presence of indole alkaloids, however not known pharmacologically active alkaloids or derivatives used in semisynthesis (vinblastine, ajmalicine, serpentine, yohimbine, vindoline and catharanthine) have been found. The ethanolic extract of <em>C. roseus</em> callus inhibited the number of mitoses in <em>Allium cepa</em> root tip cells. Short (1-3 hours) treatment resulted in an increase in the index of late prophases, with characteristic light spaces, and the index of metaphases with twisted chromosomes forming an equatorial plate or irregular structures of c-metaphases. At the same time, the percentage of anaphases and telophases decreased significantly. Longer treatment of the root tip inhibited mitotic activity, stopping it completely already after 12 hours. Interphase nuclei became at first denser and homogeneous, eventually their structure became , partitioned into zones and formation of chromatin territories, with distinct large nucleoli has been observed. Electron microscope observations revealed well developed rough endoplasmic reticulum and thick, invaginating cell membrane.

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