IDENTIFICATION OF CULTIVARS OF FORAGE LEGUME (Desmodium ovalifolium GUILL ET PERR.) BY THEIR ELECTROPHORETIC PATTERNS

1987 ◽  
Vol 67 (3) ◽  
pp. 713-717 ◽  
Author(s):  
A. HUSSAIN ◽  
W. BUSHUK ◽  
H. RAMIREZ ◽  
W. ROCA
Keyword(s):  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel ◽  
Forage Legume ◽  
Desmodium Ovalifolium ◽  
Electrophoretic Patterns ◽  
Electrophoretic Procedure

An electrophoretic procedure was developed for discriminating cultivars of Desmodium ovalifolium on the basis of patterns of partially purified seed proteins. Electrophoresis was done on uniform 15% polycrylamide gels in basic (8.9) pH. The method produced satisfactory discrimination of eight cultivars used in its initial evaluation.Key words: Forage legume, Desmodium ovalifolium Guill et Perr., cultivar identification, polyacrylamide gel electrophoresis

DISCRIMINATION OF CULTIVARS OF LENTIL (Lens culinaris Medic.) BY ELECTROPHORESIS OF SEED PROTEINS

1989 ◽  
Vol 69 (1) ◽  
pp. 243-246 ◽  
Author(s):  
A. HUSSAIN ◽  
W. BUSHUK ◽  
K. W. CLARK
Keyword(s):  
Gel Electrophoresis ◽  
Lens Culinaris ◽  
Seed Protein ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel

Discrimination of lentil cultivars was achieved by analysis of seed protein by two types of polyacrylamide gel electrophoresis. Cultivars of lentil were discriminated by the presence or absence of diagnostic bands. Electrophoregrams of six seed lots of the cultivar Eston were identical and unaffected by growing conditions.Key words: Lens culinaris Medic, seed proteins, polyacrylamide gel electrophoresis, cultivar identification

Polyacrylamide gel electrophoresis and infrared spectroscopy of vibrio biotypes

1981 ◽  
Vol 27 (10) ◽  
pp. 1048-1052 ◽  
Author(s):  
M. Maiti ◽  
P. Sur ◽  
S. N. Chatterjee
Keyword(s):  
Infrared Spectroscopy ◽  
Vibrio Cholerae ◽  
Infrared Spectra ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Electrophoretic Patterns ◽  
Different Strains ◽  
El Tor

Polyacrylamide gel electrophoresis and infrared spectroscopy were used to study the relationship between Vibrio cholerae (classical), Vibrio cholerae (El Tor), and nonagglutinable (NAG) vibrios. Acid extracts of the different strains produced type-specific electrophoretic patterns, and the infrared spectra revealed broad absorption maxima which largely correspond to those found in other organisms. With the exception of the NAG vibrios, the infrared spectra of cholera El Tor vibrios were identical. Strain-specific differences were found in the exoprotein spectra of these organisms by the sodium dodecyl sulphate – polyacrylamide gel electrophoretic technique.

Inheritance and distribution of variation at four avenin loci in North American oat germ plasm

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 416-424 ◽  
Author(s):  
E. Souza ◽  
M. E. Sorrells
Keyword(s):  
North American ◽  
Gel Electrophoresis ◽  
Germ Plasm ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
High Association ◽  
Electrophoretic Mobilities ◽  
Oat Cultivars ◽  
Taxonomic Studies

Avenins (prolamines of the Avena genus) have been shown to be useful in taxonomic studies and cultivar identification; specific allelic identification could assist in these types of studies as well as providing a basis for future linkage and gene mapping studies. The avenin patterns produced by nondenaturing polyacrylamide gel electrophoresis were compared in 70 North American oat cultivars and germ plasms. Populations of F2 progeny were subsequently evaluated to test for allelism of proteins found to be noncoincident in the survey of homozygous cultivars. A minimum of four loci (Av1, Av2, Av3, and Av4) were found to possess alternate alleles with distinctive electrophoretic mobilities. Segregation of 10 alternate alleles were observed in studies of F2 progeny: four for Av1, and two each for the other three loci. Additional variation found among the surveyed cultivars suggested at least two additional electrophoretically variant polypeptides. Several of the alleles were found to be associated with cultivars from specific geographic regions. Two examples were (i) the near exclusive association of the Av10.76 allele with Canadian cultivars and (ii) the high association of the Av10.58 allele with fall-planted cultivars. Fifty percent (SE ± 10.7%) of the fall-planted cultivars have the Av40.58 allele compared with 27.1% (SE ± 8.8%) of spring-planted cultivars.Key words: avenins, prolamines, polyacrylamide gel electrophoresis, linkage.

INFLUENCE OF SOURCE OF WHEAT CYTOPLASM ON THE NATURE OF PROTEINS IN HEXAPLOID TRITICALE

1974 ◽  
Vol 16 (3) ◽  
pp. 529-537 ◽  
Author(s):  
S. L. K. Hsam ◽  
E. N. Larter
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Proteins ◽  
Triticum Aestivum L ◽  
The Other ◽  
Hexaploid Triticale ◽  
Electrophoretic Patterns ◽  
High Molecular Weight Proteins

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to study seed proteins in 4 pairs of reciprocal F1 isogenic hybrids of hexaploid triticales differing only in their source of cytoplasm. One member of each reciprocal pair possessed the cytoplasm of hexaploid (6x) wheat (Triticum aestivum L. em. Thell), the other, the cytoplasm from tetraploid (4x) wheat (T. turgidum L). Qualitative as well as quantitative differences were observed in the electrophoretic patterns of the albumins and globulins. High molecular weight proteins (> 34,000 daltons) were synthesized in triticale with 6x wheat cytoplasm in greater quantity than in triticale with 4x wheat cytoplasm. Differences in the patterns of gliadin and reduced glutenin of the reciprocal triticale populations were quantitative. The relevance of these findings to seed development in triticales is discussed.

GLIADIN COMPOSITION OF THE BREAD-WHEAT CULTIVARS BW 20 AND SINTON

1979 ◽  
Vol 59 (4) ◽  
pp. 1001-1005 ◽  
Author(s):  
F. G. KOSMOLAK
Keyword(s):  
Bread Wheat ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Test Plot ◽  
Wheat Cultivars ◽  
Electrophoretic Patterns ◽  
Yield Test

Gliadins from 123 breeder lines of the cultivar BW 20 and from 198 breeder lines of the cultivar Sinton were extracted and separated by polyacrylamide gel electrophoresis. The cultivar BW 20 consisted of four biotypes and the cultivar Sinton of five with respect to their gliadin composition. The major biotype was represented by 93% of the breeder lines of the cultivar BW 20 and by 98% of the cultivar Sinton. The electrophoretic patterns of awned off-type plants in a yield test plot of Cultivar BW 20 were different from the patterns of the biotypes present in breeder lines of BW 20. It was concluded that these awned plants were contaminants from other cultivar sources.

IDENTIFICATION OF CICER MILKVETCH CULTIVARS USING SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS

1983 ◽  
Vol 63 (4) ◽  
pp. 1087-1090 ◽  
Author(s):  
ROBERT K. EVANS ◽  
ROLLIN H. ABERNETHY
Keyword(s):  
Banding Pattern ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel ◽  
Forage Legumes ◽  
Electrophoretic Method ◽  
Stand Establishment ◽  
Astragalus Cicer ◽  
Varietal Purity

With the release of Monarch cicer milkvetch (Astragalus cicer L.), which has greatly facilitated stand establishment when compared to older cultivars, the desirability of a method for identifying seed of cultivars was recognized. An SDS-polyacrylamide gel electrophoretic method was developed to differentiate cicer milkvetch cultivars. Densitometric scanning of the resultant polypeptide banding pattern on the polyacrylamide gel provided identification of seed lots of the cultivars Oxley, Lutana and Monarch produced in several different years and locations.Key words: Astragalus cicer L., forage legumes, seed proteins, varietal purity

scholarly journals Polyacrylamide gel electrophoresis of soybean seed proteins

2015 ◽  
Vol 47 (4) ◽  
pp. 371-378 ◽  
Author(s):  
W. Lassocińsk ◽  
J. S. Knypl
Keyword(s):  
Glycine Max ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Soybean Seed ◽  
Seed Proteins ◽  
Soluble Proteins ◽  
Polyacrylamide Gel ◽  
Major Protein ◽  
Molecular Weights ◽  
Minor Protein

Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were detected under dissociating conditions. Molecular weights of three major protein fractions in PAGE SDS were determined for around 18 500, 36 000 and 80 000 daltons.

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