In vitro selection for improved seedling vigour in birdsfoot trefoil (Lotus corniculatus L.)

1997 ◽  
Vol 77 (3) ◽  
pp. 385-390 ◽  
Author(s):  
N. L. McLean ◽  
J. Nowak

Establishment of productive stands of birdsfoot trefoil (Lotus corniculatus L.) is often difficult due to poor seedling vigour. Objectives of this study were to evaluate the relative effectiveness of in vitro selection conditions for seedling vigour and to choose the best lines for further evaluation. Birdsfoot trefoil seedlings from cvs. Leo and Upstart were selected on the basis of in vitro rate of germination at 5, 12 and 20 °C and subsequent seedling growth at 20 °C. Two cycles of recurrent selection were carried out. Progeny from both cycles of selection were evaluated under controlled and field conditions. In general, selection temperatures did not significantly affect emergence, individual shoot dry matter production or total shoot dry matter production. Emergence and total shoot dry matter yield were higher for selections within cv. Upstart than for selections within cv. Leo. Seedling vigour of cycle-2 selections was not superior to cycle-1 selections. Failure to make gains during the second cycle of selection is believed to be due to seed sterilization procedures during cycle-2 selection. Germplasms chosen for further evaluation include the 12 °C and 20 °C cycle-1 selections and the 20 °C cycle-2 selection from cv. Upstart. Key words: Lotus corniculatus L., birdsfoot trefoil, seedling vigour, in vitro selection, temperature

1983 ◽  
Vol 63 (1) ◽  
pp. 267-276 ◽  
Author(s):  
R. L. NICHOLS ◽  
R. A. PETERS

Birdsfoot trefoil (Lotus corniculatus L. ’Viking’) was seeded directly into a predominantly orchardgrass (Dactylis glomerata L.) sward following overall or banded applications of paraquat (1,1′-dimethyl-4, 4′-bipyridinium ion, as dichloride salt) or glyphosate [N-(phosphonomethyl)glycine, as isopropylamine salt] to determine the degree of sward control necessary for stand establishment without tillage. Effects of the herbicide treatments and the seeding on botanical composition and dry matter production were determined by a vertical intercept technique and by hand separations of harvests into botanical components. Trefoil establishment was proportional to the degree of sward control during the seeding year. Little trefoil was established by direct seeding without herbicides. The banded herbicide treatments were less effective than overall herbicide applications for trefoil establishment. Trefoil yield obtained following overall application of paraquat was approximately 65% of that obtained following overall glyphosate treatment.Key words: Dactylis glomerata, glyphosate, Lotus corniculatus, herbicide, no-tillage, paraquat


1969 ◽  
Vol 49 (3) ◽  
pp. 339-349 ◽  
Author(s):  
H. Gasser ◽  
L. Lachance ◽  
P. Gervais

In two experiments, one with alfalfa and the other with birdsfoot trefoil, we have been able to show that a late cut in October does not adversely affect dry matter yield (DAI) of the legumes. Three cuts during a harvesting season ending on September 5 were detrimental to alfalfa, since lower yields were obtained the following year of harvest. Birdsfoot trefoil varieties were affected differentially. Three cuts did not affect the yield of Viking the following year, but did so of Empire. Significant differences in dry matter yields were obtained between DuPuits and Vernal and between Viking and Empire. Protein in the forage followed inversely the same pattern as that of DM yields, that is, where the intervals were shortest, the protein content was highest, and conversely. The total available carbohydrate and the nitrogen content of the roots were lowest following the treatments which had the shortest intervals between them.


1988 ◽  
Vol 68 (3) ◽  
pp. 751-760 ◽  
Author(s):  
J. CHIQUETTE ◽  
K.-J. CHENG ◽  
J. W. COSTERTON ◽  
L. P. MILLIGAN

Two new isosynthetic strains of birdsfoot trefoil (Lotus corniculatus L.), containing low or high concentrations of tannin, were tested using in vitro and in sacco digestibility techniques. The high tannin (HT) strain contained an average of five times more tannin on a dry matter basis, than the low tannin (LT) strain. After 24-h in vitro digestion, by a rumen inoculum, there was less (P < 0.01) dry matter disappearance (45.7 vs. 54.4%) from the HT strain. Also, the development of gas pressure was lower (P < 0.01) and production of volatile fatty acids less (P < 0.05) with the HT strain than with the LT strain. Average dry matter loss from four replicated nylon bags was also lower (P < 0.01) for the HT strain than for the LT strain after 8, 12, and 24 h of incubation in rumen. These data indicate that a LT strain of birdsfoot trefoil might improve the forage digestibility. Key words: Birdsfoot trefoil, tannin, digestibility


2017 ◽  
Vol 38 (3) ◽  
pp. 1483
Author(s):  
Loreno Egidio Taffarel ◽  
Paulo Sérgio Rabello de Oliveira ◽  
Euclides Reuter de Oliveira ◽  
Elaine Barbosa Muniz ◽  
Eduardo Eustáquio Mesquita ◽  
...  

Morphological characteristics, dry matter production, and nutritional values of winter forage and grains were evaluated. This study was conducted from April 24, 2012 to November 7, 2013 in the Western Paraná State University (UNIOESTE), Marechal Cândido Rondon, Brazil. Pastures under one grazing and non-grazing conditions were evaluated under 120 kg N ha-1 fertilization split into two 60 kg N ha-1 treatments. Two pastures received 40 kg N ha-1 three times. IPR 126 oat, BRS Tarumã wheat, and IPR 111 triticale were the test crops. Topdressing with 40 or 60 kg N ha-1 did not change morphological characteristics until 60 d after sowing. Pastures under non-grazing that received 120 kg N ha-1 treatments were taller than the controls, whereas those under grazing that received 80 or 120 kg N ha-1 presented with higher leaf production than did the controls. Total average dry matter (DM) production in 2012 and 2013 was, respectively, 5,275 kg ha-1 and 6,270 kg ha-1 for oat, 3,166 kg ha-1 and 7,423 kg ha-1 for wheat, and 4,552 kg ha-1 and 7,603 kg ha-1 for triticale. Split N fertilization did not cause differences in the levels of crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) in the forage. Nevertheless, increases in in vitro dry matter digestibility (IVDMD) were observed in oat and wheat receiving 60 kg N ha-1 during the first graze. IVDMD did not change in oat, wheat, and triticale forages receiving 80 or 120 kg N ha-1 during the second graze. Grazing did not affect the nutritional values of wheat and triticale grains, but reduced those of oat. Therefore, the results of the present study suggest that grazing lengthens the crop cycles, and so allow the staggered sowing of summer crops.


2007 ◽  
Vol 58 (3) ◽  
pp. 225 ◽  
Author(s):  
M. E. Rogers

The response of 4 temperate grass species (Lolium perenne cv. Victorian, Thinopyrum ponticum cv. Tyrell, Austrodanthonia richardsonii cv. Taranna, A. bipartita cv. Bunderra) to saline irrigated conditions was evaluated over 4 seasons at Tatura in northern Victoria. This experiment followed earlier research where the salt tolerance of ~20 species of grasses was evaluated in the greenhouse. Field plots were established under non-saline conditions and were irrigated with saline water at 1.6, 2.5, and 4.5 dS/m. Measurements made on these plots included dry-matter production, tissue ion (Na+, Cl–, K+, Mg2+, Ca2+) concentrations, in vitro dry-matter digestibility, root distribution, and soil chemistry. Soil salinity (EC1 : 5) and sodicity (SAR1 : 5) levels peaked at 0.30–0.60 m depth and reached 1.3 dS/m and 9.8, respectively, for the highest saline irrigation treatment. Cumulative plant dry-matter production was lower in T. ponticum compared with the Austrodanthonia species and L. perenne at all salinity levels, but in relative terms there was no difference in the salt tolerance among any of the 4 species (the reduction in dry weight at 4.5 dS/m was 10–15% for all species). Leaf tissue concentrations of Na+ and Cl– were significantly lower in A. richardsonii and A. bipartita compared with T. ponticum and L. perenne, and in vitro dry-matter digestibility tended to be greater in L. perenne under saline conditions than in the other 3 species. This research suggests that the 2 native Austrodanthonia species can be grown under moderately saline conditions—either under saline irrigation or in a dryland discharge area—in environments where perennial ryegrass may also be grown.


1985 ◽  
Vol 65 (2) ◽  
pp. 423-433 ◽  
Author(s):  
G. BELANGER ◽  
J. E. WINCH ◽  
J. L. TOWNSHEND

Field experiments were conducted to determine the effects of carbofuran on the establishment and subsequent yields of sod-seeded legumes. The effects of carbofuran on plant growth, nodulation and nitrogen fixation of birdsfoot trefoil (Lotus corniculatus L.) in non-sterilized and sterilized soil were evaluated in growth cabinet experiments. In the field experiments carbofuran, applied immediately after seeding, had a positive effect on plant stand and seedling dry matter production of birdsfoot trefoil and alfalfa (Medicago sativa L. ssp. falcata L.) in the weeks following seeding. In the first production years of these field trials, there was a trend for the total and legume yields to be higher in the carbofuran plots than where carbofuran was not used. In either growth cabinet experiment, however, shoot growth was not affected by carbofuran but a reduction in the number of plants in the non-sterilized and carbofuran pots occurred. A toxic effect of carbofuran on seedlings may have been involved. No correlation between soil nematode numbers and plant stand or dry matter production was found in either field or growth cabinet experiments. In the growth cabinet experiments, carbofuran did not affect the number of nodules but improved nitrogen fixation in sterilized pots of exp. B. Carbofuran increased the dry weight of individual nodules in the sterilized pots of exp. A but a decrease was observed in exp. B. These effects of carbofuran on the dry weight of individual nodules and specific nodule activity were also observed in the nematode-free environment created by sterilization. In view of these responses in sterile soil, it was suggested that the effect of carbofuran was not as a nematicide but rather a positive physiological effect on the plant component of the plant-rhizobium system.Key words: Carbofuran, establishment, legumes, nitrogen fixation, nematodes


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