scholarly journals Biotic and abiotic stress responses through calcium-dependent protein kinase (CDPK) signaling in wheat (Triticum aestivumL.)

2008 ◽  
Vol 3 (9) ◽  
pp. 654-656 ◽  
Author(s):  
Aili Li ◽  
Xiang Wang ◽  
Charles H. Leseberg ◽  
Jizeng Jia ◽  
Long Mao
Keyword(s):  
Abiotic Stress ◽  
Protein Kinase ◽  
Stress Responses ◽  
Biotic And Abiotic Stress ◽  
Dependent Protein Kinase ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinase

scholarly journals Prioritization of abiotic and biotic stress responses by direct linkage of ABI1 phosphatase and CPK5 calcium-dependent protein kinase

2019 ◽  
Author(s):  
Heike Seybold ◽  
Jennifer Bortlik ◽  
Benjamin Conrads ◽  
Wolfgang Hoehenwarter ◽  
Tina Romeis
Keyword(s):  
Abiotic Stress ◽  
Protein Kinase ◽  
Immune Responses ◽  
Stress Responses ◽  
Negative Regulator ◽  
Dependent Protein Kinase ◽  
Immune Signaling ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinase

AbstractIn nature plants are constantly challenged by simultaneous abiotic and biotic stresses, and under conflicting stress scenarios prioritization of stress responses is required for plant survival. Calcium-dependent protein kinase CPK5 is a central hub in local and distal immune signaling, required upstream of hormone salicylic acid (SA)-dependent systemic acquired resistance (SAR). Here we show that CPK5 signaling-dependent immune responses are effectively blocked and pathogen resistance is reverted either upon treatment of plants with abscisic acid (ABA) or in genetic mutant backgrounds lacking PP2C phosphatase activities including abi1-2. Consistently, enhanced immune responses occur upon co-expression of CPK5 kinase with active variants of ABI1 phosphatase ABI1G180S and ABI1G181A. Biochemical studies and mass spectrometry-based phosphosite analysis reveal a direct ABI1 phosphatase-catalyzed de-phosphorylation of CPK5 at T98, a CPK5 auto-phosphorylation site. CPK5T98A, mimicking continuous de-phosphorylation through ABI1, correlates with an increase in kinase activity and CPK5 function in ROS production. CPK5T98D, mimicking a CPK5 auto-phosphorylated status under ABA-induced phosphatase inhibition, leads to inactivated CPK5 causative to an immediate stop of immune responses.Our work reveals an elegant mechanism for plant stress prioritization, where the ABA-dependent phosphatase ABI1, negative regulator of abiotic responses, functions as positive regulator of biotic stress responses, stabilizing CPK5-dependent immune responses in the absence of ABA. This mechanism allows continuous immune signaling during pathogen survey in environmentally non-challenging conditions. Under severe abiotic stress, immune signaling is discontinued via a direct biochemical intersection through a phosphatase/kinase pair recruiting two key regulatory enzymes of these antagonistic signaling pathways.Significance StatementPlants challenged by simultaneous abiotic and biotic stresses prioritize in conflicting scenarios to guarantee survival. Systemic acquired resistance (SAR), a plant’s immune memory, depends on the hormone salicylic acid (SA) and prepares a plant for future pathogen attack. Adaptation to abiotic stress signaling involves the hormone abscisic acid (ABA). We identify a direct biochemical mechanism by which ABA-mediated abiotic signaling prioritizes over immune responses via reversible phosphorylation involving two key regulatory enzymes of these antagonistic pathways. Phosphatase ABI1, negative regulator of abiotic stress signaling, de-phosphorylates calcium-dependent protein kinase CPK5 at an auto-phosphorylation protein mark, leading to kinase activation and function in immune signaling. Under abiotic stress ABA-dependent phosphatase inhibition results in continuous phosphorylated inactive kinase, preventing immune signaling.

scholarly journals Calcium-Dependent Protein Kinase CPK21 Functions in Abiotic Stress Response in Arabidopsis thaliana

2011 ◽  
Vol 4 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Sandra Franz ◽  
Britta Ehlert ◽  
Anja Liese ◽  
Joachim Kurth ◽  
Anne-Claire Cazalé ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Stress Response ◽  
Protein Kinase ◽  
Abiotic Stress Response ◽  
Dependent Protein Kinase ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinase

scholarly journals A Novel Calcium-Dependent Protein Kinase 1 Inhibitor Potently Prevents Toxoplasma gondii Transmission to Foetuses in Mouse

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4203
Author(s):  
Héloïse Débare ◽  
Nathalie Moiré ◽  
Firmin Baron ◽  
Louis Lantier ◽  
Bruno Héraut ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Toxoplasma Gondii ◽  
Intraperitoneal Administration ◽  
Congenital Toxoplasmosis ◽  
Parasite Burden ◽  
Oral Route ◽  
Dependent Protein Kinase ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinase

Treatments currently used to prevent congenital toxoplasmosis are non-specific of Toxoplasma gondii and have grievous side effects. To develop a more specific and less toxic drug, we have designed SP230, an imidazo[1,2-b]pyridazine salt targeting the Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) and active against acute toxoplasmosis in mice. Efficiency of SP230 to inhibit foetal transmission of the parasite was evaluated in a mouse model of congenital toxoplasmosis. Swiss mice were infected at mid-pregnancy with tachyzoites or cysts of the ME49 strain of T. gondii by intraperitoneal and oral route, respectively, and treated with SP230 at 50 mg/kg for 5 days by the same routes. Parasite burden in organs of dams and in foetuses was measured by quantitative PCR. Intraperitoneal administration of SP230 drastically reduced the number of parasites (more than 97% of reduction) in the brain and lungs of dams, and led to a reduction of 66% of parasite burden in foetuses. Oral administration of SP230 was particularly efficient with 97% of reduction of parasite burdens in foetuses. SP230 did not impact number and weight of offspring in our conditions. This inhibitor of TgCDPK1 is a promising candidate for the development of alternative therapeutics to treat infected pregnant women.

scholarly journals The Evolutionary History and Diverse Physiological Roles of the Grapevine Calcium-Dependent Protein Kinase Gene Family

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e80818 ◽  
Author(s):  
Fei Chen ◽  
Marianna Fasoli ◽  
Giovanni Battista Tornielli ◽  
Silvia Dal Santo ◽  
Mario Pezzotti ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Gene Family ◽  
Evolutionary History ◽  
Dependent Protein Kinase ◽  
Kinase Gene ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Protein Kinase Gene ◽  
Calcium Dependent Protein Kinase

scholarly journals Cross-talk of calcium-dependent protein kinase and MAP kinase signaling

2011 ◽  
Vol 6 (1) ◽  
pp. 8-12 ◽  
Author(s):  
Bernhard Wurzinger ◽  
Andrea Mair ◽  
Barbara Pfister ◽  
Markus Teige
Keyword(s):  
Protein Kinase ◽  
Map Kinase ◽  
Cross Talk ◽  
Kinase Signaling ◽  
Dependent Protein Kinase ◽  
Calcium Dependent ◽  
Map Kinase Signaling ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinase
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