Elevated Serum Antibody Levels against Cyclin L2 in Patients with Esophageal Squamous Cell Carcinomai

Haemophilus influenzae type b capsular polysaccharide vaccine was given in Finland in 1974 to approximately 50,000 infants and children, whose serum anti-H influenzae type b capsular polysaccharide levels have been followed for 3½ years. The serum antibodies induced by the vaccination proved short-lived (less than 6 months) in the infants younger than 18 months. Elevated serum antibody levels were detectable for 1½ years but less than 3½ years in the children who were vaccinated when 18 to 35 months old. In the children who were 3 to 5 years old when vaccinated, the elevated anti-H influenzae type b capsular polysaccharide levels persisted for at least 3½ years. Therefore, children vaccinated at the age of 18 months may need a new dose of vaccine 1 to 1½ years after the first dose in order to be protected for the period of high susceptibility, until the age of approximately 7 years. Some of the vaccinated children were reimmunized 3½ years after the first dose, and the anti-H influenzae type b capsular polysaccharide levels in their sera were studied in a similar manner. At no time did the anti-H influenzae type b capsular polysaccharide levels after the reimmunization differ from the anti-H influenzae type b capsular polysaccharide levels seen after the first vaccination in children of the same age. In addition, the children who had received their first dose of vaccine when younger than 18 months and therefore were not responding, responded now. Thus, there was no evidence of immunologic tolerance.

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Immune response in urinary tract infection determined by radioimmunoassay and immunofluorescence: serum antibody levels against infecting bacterium and Enterobacteriaceae common antigen

Journal of Clinical Microbiology ◽

10.1128/jcm.8.5.575-579.1978 ◽

1978 ◽

Vol 8 (5) ◽

pp. 575-579

Author(s):

B A Sanford ◽

V L Thomas ◽

M Forland ◽

S Carson ◽

A Shelokov

Keyword(s):

Urinary Tract ◽

Solid Phase ◽

Fluorescent Antibody ◽

Serum Antibody ◽

Elevated Serum ◽

Antibody Activity ◽

Common Antigen ◽

Enterobacterial Common Antigen ◽

Tract Infections ◽

Antibody Levels

A solid-phase radioimmunoassay (RIA) procedure was compared with the indirect fluorescent antibody (IFA) test in a serological study of 76 female adults with urinary tract infections. Relative serum antibody activity was determined against patients' homologous infecting enterobacteria by RIA and IFA and against heterologous enterobacterial common antigen (Escherichia coli O14) by RIA. There was marked correlation between results of the IFA and RIA methods using the homologous system; 22 of 51 patients (43%) with pyelonephritis had significantly elevated serum antibody activity by both IFA (titers greater than or equal to 512) and RIA (binding ratio greater than or equal to 2.0) when compared with normal serum controls; three had significant antibody activity detectable by RIA only. Eighteen (72%) of 25 patients with pyelonephritis had RIA binding ratios of greater than or equal to 2.0 against their homologous bacterial isolates and the enterobacterial common antigen; an additional 6 patients had binding ratios of greater than or equal to 2.0 against the antigen only. All 25 patients with cystitis had low serum antibody levels by IFA and RIA when tested against their own isolate as well as enterobacterial common antigen. The RIA procedure was objective, quantitative, and less tedious to perform than IFA.

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Antibody response of carp, Cyprinus carpio to the cestode, Bothriocephalus acheilognathi

Parasitology ◽

10.1017/s0031182099004242 ◽

1999 ◽

Vol 118 (6) ◽

pp. 635-639 ◽

Cited By ~ 11

Author(s):

P. NIE ◽

D. HOOLE

Keyword(s):

Antibody Response ◽

Cyprinus Carpio ◽

Serum Antibody ◽

Infected Fish ◽

Immune Protection ◽

Carp Cyprinus Carpio ◽

Bothriocephalus Acheilognathi ◽

Protection Against Infection ◽

Antibody Levels ◽

Antibody Secreting Cells

The humoral antibody response and the number of pronephric antibody-secreting cells were examined in naturally Bothriocephalus acheilognathi-infected carp. Cyprinus carpio, and in those injected intraperitoneally with an extract of the cestode. In the extract-injected fish, specific antibody was detected 3 weeks after a second injection given 2 weeks after the primary injection, and antibody levels persisted for more than 200 days. A third injection also enhanced the antibody level in the extract-injected carp. The numbers of antibody-secreting cells were significantly higher in carp injected 3 times with the extract than in the control. In naturally-infected fish, the serum antibody levels and the number of pronephric antibody-secreting cells were higher in infected fish than in uninfected individuals although this difference was not statistically significant. The relevance of these results to immune protection against infection is discussed.

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Low Immunogenicity of Intravesical Phage Therapy for Urogenitary Tract Infections

Antibiotics ◽

10.3390/antibiotics10060627 ◽

2021 ◽

Vol 10 (6) ◽

pp. 627

Author(s):

Sławomir Letkiewicz ◽

Marzanna Łusiak-Szelachowska ◽

Ryszard Międzybrodzki ◽

Maciej Żaczek ◽

Beata Weber-Dąbrowska ◽

...

Keyword(s):

Clinical Trial ◽

Bacterial Infections ◽

Phage Therapy ◽

Serum Antibody ◽

Multidrug Resistant ◽

Antibody Responses ◽

Urogenital Infections ◽

Reliable Model ◽

Tract Infections ◽

Antibody Levels

Patients with chronic urinary and urogenital multidrug resistant bacterial infections received phage therapy (PT) using intravesical or intravesical and intravaginal phage administration. A single course of PT did not induce significant serum antibody responses against administered phage. Whilst the second cycle of PT caused a significant increase in antibody levels, they nevertheless remained quite low. These data combined with good therapy results achieved in some patients suggest that this mode of PT may be an efficient means of therapy for urogenital infections and a reliable model for a clinical trial of PT.

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Primary squamous cell carcinoma of the pancreas with a large pseudocyst of the pancreas as the first manifestation: a rare case report and literature review

BMC Gastroenterology ◽

10.1186/s12876-021-01804-7 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Xia Qiu ◽

Yajie Meng ◽

Meiqin Lu ◽

Chuan Tian ◽

Min Wang ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Cell Mass ◽

Elevated Serum ◽

Abdominal Distension ◽

Needle Aspiration ◽

Pancreatic Pseudocysts ◽

Primary Squamous Cell Carcinoma ◽

Ki 67

Abstract Background Primary squamous cell carcinoma (SCC) of the pancreas with pseudocysts, especially diagnosed by endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA), is extremely rare. Case presentation A 64-year-old man was admitted to our department for abdominal distension. Two months ago, he experienced abdominal pain for 1 day and was diagnosed with acute pancreatitis in another hospital. After admission, laboratory tests showed the following: amylase 400 U/L, lipase 403 U/L, and carbohydrate antigen 19–9 (CA19-9) 347 U/mL. Abdominal computed tomography (CT) revealed pancreatitis with a pseudocyst with a diameter measuring 7 cm. During linear EUS, a large pseudocyst (5.4 × 5.2 cm) was observed in the pancreatic body. EUS-FNA was performed. We obtained specimens for histopathology and placed a plastic stent through the pancreas and stomach to drain the pseudocyst. Puncture fluid examination revealed the following: CA19-9 > 12,000 U/mL carcinoembryonic antigen (CEA) 7097.42 ng/ml, amylase 27,145.3 U/L, and lipase > 6000 U/L. Cytopathology revealed an abnormal cell mass, and cancer was suspected. Furthermore, with the result of immunohistochemistry on cell mass (CK ( +), P40 ( +), p63 ( +), CK7 (−) and Ki-67 (30%)), the patient was examined as squamous cell carcinoma (SCC). However, the patient refused surgery, radiotherapy and chemotherapy. After drainage, the cyst shrank, but the patient died 3 months after diagnosis due to liver metastasis and multiple organ failure. Conclusion For patients with primary pancreatic pseudocysts with elevated serum CEA and CA19-9 levels, we should not rule out pancreatic cancer, which may also be a manifestation of primary pancreatic SCC. EUS-FNA is helpful for obtaining histopathology and cytology and thus improving diagnostic accuracy.

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