Enzymatic production of pectin-derived oligosaccharides from sugar beet pulp as a component of animal feed

Over 8 million tonnes of sugar beet are grown annually in the UK. Sugar beet pulp (SBP) is the main by-product of sugar beet processing which is currently dried and sold as a low value animal feed. SBP is a rich source of carbohydrates, mainly in the form of cellulose and pectin, including d-glucose (Glu), l-arabinose (Ara) and d-galacturonic acid (GalAc). This work describes the technical feasibility of an integrated biorefinery concept for the fractionation of SBP and conversion of these monosaccharides into value-added products. SBP fractionation is initially carried out by steam explosion under mild conditions to yield soluble pectin and insoluble cellulose fractions. The cellulose is readily hydrolysed by cellulases to release Glu that can then be fermented by a commercial yeast strain to produce bioethanol at a high yield. The pectin fraction can be either fully hydrolysed, using physico-chemical methods, or selectively hydrolysed, using cloned arabinases and galacturonases, to yield Ara-rich and GalAc-rich streams. These monomers can be separated using either Centrifugal Partition Chromatography (CPC) or ultrafiltration into streams suitable for subsequent enzymatic upgrading. Building on our previous experience with transketolase (TK) and transaminase (TAm) enzymes, the conversion of Ara and GalAc into higher value products was explored. In particular the conversion of Ara into l-gluco-heptulose (GluHep), that has potential therapeutic applications in hypoglycaemia and cancer, using a mutant TK is described. Preliminary studies with TAm also suggest GluHep can be selectively aminated to the corresponding chiral aminopolyol. The current work is addressing the upgrading of the remaining SBP monomer, GalAc, and the modelling of the biorefinery concept to enable economic and Life Cycle Analysis (LCA).

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Sugar beet pulp as biomass

Sugar Industry ◽

10.36961/si18105 ◽

2017 ◽

pp. 29-32 ◽

Cited By ~ 1

Author(s):

Kazm Eber Özba ◽

Özen Özboy Özba

Keyword(s):

Sugar Beet ◽

Animal Feed ◽

Sugar Industry ◽

Low Cost ◽

Industrial Applications ◽

Single Cell Protein ◽

Sugar Beet Pulp ◽

Raw Material ◽

Cell Protein ◽

Beet Pulp

The sugar beet industry produces considerable amounts of organic waste and by-products. Sugar beet pulp (SBP) is the residue that remains after sugar extraction. SBP is a lignocellulosic by-product of the sugar industry and generally used as animal feed at relatively low price. Instead of cattle feeding, SBP can be used as a raw material for industrial applications because it is low-cost and available in large amounts. Biomass is a clean and renewable energy source. The use of SBP for the production of ethanol, methanol, single cell protein, biofuels etc. is economically very attractive. This literature review evaluates the use of SBP as biomass.

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A Simple Biorefinery Concept to Produce 2G-Lactic Acid from Sugar Beet Pulp (SBP): A High-Value Target Approach to Valorize a Waste Stream

Molecules ◽

10.3390/molecules25092113 ◽

2020 ◽

Vol 25 (9) ◽

pp. 2113 ◽

Cited By ~ 2

Author(s):

Regiane Alves de Oliveira ◽

Roland Schneider ◽

Betânia Hoss Lunelli ◽

Carlos Eduardo Vaz Rossell ◽

Rubens Maciel Filho ◽

...

Keyword(s):

Lactic Acid ◽

Sugar Beet ◽

Animal Feed ◽

Continuous Fermentation ◽

Bacillus Coagulans ◽

Sugar Beet Pulp ◽

Waste Stream ◽

Production Chain ◽

Vast Number ◽

Beet Pulp

Lactic acid is a high-value molecule with a vast number of applications. Its production in the biorefineries model is a possibility for this sector to aggregate value to its production chain. Thus, this investigation presents a biorefinery model based on the traditional sugar beet industry proposing an approach to produce lactic acid from a waste stream. Sugar beet is used to produce sugar and ethanol, and the remaining pulp is sent to animal feed. Using Bacillus coagulans in a continuous fermentation, 2781.01 g of lactic acid was produced from 3916.91 g of sugars from hydrolyzed sugar beet pulp, with a maximum productivity of 18.06 g L−1h−1. Without interfering in the sugar production, ethanol, or lactic acid, it is also possible to produce pectin and phenolic compounds in the biorefinery. The lactic acid produced was purified by a bipolar membrane electrodialysis and the recovery reached 788.80 g/L with 98% w/w purity.

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Researches on the higher valuation of sugar beet pulp from sugar production to obtain premixes for animal feed

E3S Web of Conferences ◽

10.1051/e3sconf/201911203023 ◽

2019 ◽

Vol 112 ◽

pp. 03023

Author(s):

Elena Mihaela Nagy ◽

Constantin Coţa ◽

Nicolae Cioica ◽

Zoltan Gyorgy ◽

Teodora Deac

Keyword(s):

Sugar Beet ◽

Nutritional Value ◽

Animal Feed ◽

Sugar Beet Pulp ◽

Sugar Production ◽

Temperature Influence ◽

Beet Pulp ◽

Drying Conditions ◽

Realization Process ◽

Maximum Moisture Content

The sugar beet pulp from the sugar production is, thanks to the easily assimilable carbohydrate content, an important source of feed, especially for polygastric animals. Its efficiency in terms of assimilation and biological effect can be substantially improved by the addition of macroelements (Ca, Mg, P) and microelements (Zn, Co, Fe, Mn, Cu). These elements react with the sugar beet pulp compounds to form specific chelates. In order to preserve and maintain the nutritional value of the premix, the realization process involves a drying operation. In view of this, experiments have been carried out to determine the maximum moisture content that ensures preservation and optimum drying conditions. This paper presents how the percentage of micro-and macro-elements used and the drying temperature influence the drying speed of the premix

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Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp

Biomolecules ◽

10.3390/biom10050724 ◽

2020 ◽

Vol 10 (5) ◽

pp. 724 ◽

Cited By ~ 1

Author(s):

Agnieszka Wilkowska ◽

Joanna Berlowska ◽

Adriana Nowak ◽

Ilona Motyl ◽

Aneta Antczak-Chrobot ◽

...

Keyword(s):

Sugar Beet ◽

Pathogenic Bacteria ◽

Animal Feed ◽

Biomass Conversion ◽

Lactobacillus Brevis ◽

Feed Additives ◽

Sugar Beet Pulp ◽

Gastrointestinal Bacteria ◽

Beet Pulp

An effective and ecological method for liberation of pectin-derived oligosaccharides (POS) from sugar beet pulp (SBP) was developed using enzymatic and microorganism-mediated biomass conversion. The POS may be applied in the production of prebiotic feed additives. Various yeast strains were screened for their capacity for protein synthesis and monosaccharide assimilation. Combined yeast cultivation and pectin hydrolysis were found to be an effective method of producing prebiotics. Separate enzymatic hydrolysis and fermentation of SBP resulted in the release of 3.6 g of POS per 100 g d.w., whereas the yield of POS acquired after the combined process was 17.9% higher, giving 4.2 g of POS per 100 g d.w. Introducing the yeast into the process improved hydrolysis performance due to lower enzyme inhibition by mono- and disaccharides. The prebiotic effect of the POS was assessed by in vitro fermentation using individual cultures of gastrointestinal bacteria. The POS in the SBP hydrolysate effectively promoted the growth of lactobacilli and bifidobacteria. A large increase in adherence to Caco-2 cells in the presence of POS was noted for beneficial Lactobacillus brevis strains, whereas pathogenic bacteria and yeast (C. albicans, C. lusitanie, C. pelliculosa), responsible for infections in breeding animals, showed much weaker adhesion.

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Obtaining of sorbing material based on sugar beet pulp

Environmental Protection in Oil and Gas Complex ◽

10.33285/2411-7013-2019-6(291)-10-16 ◽

2019 ◽

pp. 10-16

Author(s):

S.V. Meshcheryakov ◽

◽

I.S. Eremin ◽

D.O. Sidorenko ◽

M.S. Kotelev ◽

...

Keyword(s):

Sugar Beet ◽

Sugar Beet Pulp ◽

Beet Pulp

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Kinetic and thermodynamic study of the adsorption of calcium onto sugar beet pulp

Sugar Industry ◽

10.36961/si17787 ◽

2016 ◽

pp. 565-570

Author(s):

Huang Qin ◽

Zhu Si-ming ◽

Zeng Di ◽

Yu Shu-juan

Keyword(s):

Sugar Beet ◽

Adsorption Equilibrium ◽

Ph Value ◽

Hard Water ◽

Sugar Beet Pulp ◽

Order Kinetic ◽

Factors Affecting ◽

Isotherm Equation ◽

Equilibrium Data ◽

Beet Pulp

Sugar beet pulp (SBP) was used as low value adsorbent for the removal of calcium from hard water. Batch experiments were conducted to determine the factors affecting adsorption of the process such as pH value and Ca concentration. The adsorption equilibrium of Ca2+ by the SBP is reached after 100min and a pseudo second-order kinetic model can describe the adsorption process. The initial concentrations of Ca varied from 927 to 1127mgCa2+/L. A dose of 30g/L sugar beet pulp was sufficient for the optimum removal of calcium. The overall uptake of Ca ions by sugar beet pulp has its maximum at pH=8. The adsorption equilibrium data fitted well with the Langmuir adsorption isotherm equation.

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Possibilities of joint treatment of spent sugar beet pulp and waste water from a sugar factory

Sugar Industry ◽

10.36961/si13694 ◽

2012 ◽

pp. 756-761 ◽

Cited By ~ 1

Author(s):

Miroslav Hutnan ◽

Štefan Tóth ◽

Igor Bodík ◽

Nina Kolesárová ◽

Michal Lazor ◽

...

Keyword(s):

Wastewater Treatment ◽

Sugar Beet ◽

Pilot Plant ◽

Biogas Production ◽

Sugar Beet Pulp ◽

Spare Capacity ◽

Anaerobic Reactor ◽

Sugar Factory ◽

Beet Pulp ◽

Joint Treatment

The possibility of joint treatment of spent sugar beet pulp and wastewater from a sugar factory was studied in this work. Works focused on processing of spent sugar beet pulp separately or together with other substrates can be found in the literature. In the case of some sugar factories, which have spare capacity in the anaerobic reactor on an anaerobic-aerobic wastewater treatment plant, joint processing of spent sugar beet pulp and wastewater from the sugar factory might be an interesting option. The results of the operation of a pilot plant of an anaerobic reactor with a capacity of 3.5 m3 are discussed. Operation of the pilot plant confirmed the possibility of cofermentation of these materials. The organic loading rate achieved in the anaerobic reactor was higher than 6 kg/(m3·d) (COD), while more than half of the load was provided by spent sugar beet pulp. The addition of sugar beet pulp decreased the concentration of ammonia nitrogen in the anaerobic reactor and it was even necessary to add nitrogen. However, the nitrogen content in sludge water depends on the C:N ratio in the processed sugar beet pulp, therefore this knowledge cannot be generalized. About 1.5 to 2-fold biogas production can be expected from the cofermentation of wastewater with sugar beet pulp in an anaerobic reactor, compared with the biogas production from just wastewater treatment.

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