scholarly journals Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 724 ◽  
Author(s):  
Agnieszka Wilkowska ◽  
Joanna Berlowska ◽  
Adriana Nowak ◽  
Ilona Motyl ◽  
Aneta Antczak-Chrobot ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Pathogenic Bacteria ◽  
Animal Feed ◽  
Biomass Conversion ◽  
Lactobacillus Brevis ◽  
Feed Additives ◽  
Sugar Beet Pulp ◽  
Gastrointestinal Bacteria ◽  
Beet Pulp

An effective and ecological method for liberation of pectin-derived oligosaccharides (POS) from sugar beet pulp (SBP) was developed using enzymatic and microorganism-mediated biomass conversion. The POS may be applied in the production of prebiotic feed additives. Various yeast strains were screened for their capacity for protein synthesis and monosaccharide assimilation. Combined yeast cultivation and pectin hydrolysis were found to be an effective method of producing prebiotics. Separate enzymatic hydrolysis and fermentation of SBP resulted in the release of 3.6 g of POS per 100 g d.w., whereas the yield of POS acquired after the combined process was 17.9% higher, giving 4.2 g of POS per 100 g d.w. Introducing the yeast into the process improved hydrolysis performance due to lower enzyme inhibition by mono- and disaccharides. The prebiotic effect of the POS was assessed by in vitro fermentation using individual cultures of gastrointestinal bacteria. The POS in the SBP hydrolysate effectively promoted the growth of lactobacilli and bifidobacteria. A large increase in adherence to Caco-2 cells in the presence of POS was noted for beneficial Lactobacillus brevis strains, whereas pathogenic bacteria and yeast (C. albicans, C. lusitanie, C. pelliculosa), responsible for infections in breeding animals, showed much weaker adhesion.

Effect of pig faecal donor and of pig diet composition on in vitro fermentation of sugar beet pulp

2007 ◽  
Vol 132 (3-4) ◽  
pp. 212-226 ◽  
Author(s):  
Jérôme Bindelle ◽  
André Buldgen ◽  
Damien Lambotte ◽  
José Wavreille ◽  
Pascal Leterme
Keyword(s):  
Sugar Beet ◽  
Diet Composition ◽  
Sugar Beet Pulp ◽  
In Vitro Fermentation ◽  
Beet Pulp

scholarly journals An integrated biorefinery concept for conversion of sugar beet pulp into value-added chemicals and pharmaceutical intermediates

2017 ◽  
Vol 202 ◽  
pp. 415-431 ◽  
Author(s):  
Max Cárdenas-Fernández ◽  
Maria Bawn ◽  
Charlotte Hamley-Bennett ◽  
Penumathsa K. V. Bharat ◽  
Fabiana Subrizi ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Animal Feed ◽  
Value Added ◽  
Sugar Beet Pulp ◽  
High Yield ◽  
Integrated Biorefinery ◽  
Physico Chemical ◽  
Beet Pulp ◽  
Commercial Yeast ◽  
The Uk

Over 8 million tonnes of sugar beet are grown annually in the UK. Sugar beet pulp (SBP) is the main by-product of sugar beet processing which is currently dried and sold as a low value animal feed. SBP is a rich source of carbohydrates, mainly in the form of cellulose and pectin, including d-glucose (Glu), l-arabinose (Ara) and d-galacturonic acid (GalAc). This work describes the technical feasibility of an integrated biorefinery concept for the fractionation of SBP and conversion of these monosaccharides into value-added products. SBP fractionation is initially carried out by steam explosion under mild conditions to yield soluble pectin and insoluble cellulose fractions. The cellulose is readily hydrolysed by cellulases to release Glu that can then be fermented by a commercial yeast strain to produce bioethanol at a high yield. The pectin fraction can be either fully hydrolysed, using physico-chemical methods, or selectively hydrolysed, using cloned arabinases and galacturonases, to yield Ara-rich and GalAc-rich streams. These monomers can be separated using either Centrifugal Partition Chromatography (CPC) or ultrafiltration into streams suitable for subsequent enzymatic upgrading. Building on our previous experience with transketolase (TK) and transaminase (TAm) enzymes, the conversion of Ara and GalAc into higher value products was explored. In particular the conversion of Ara into l-gluco-heptulose (GluHep), that has potential therapeutic applications in hypoglycaemia and cancer, using a mutant TK is described. Preliminary studies with TAm also suggest GluHep can be selectively aminated to the corresponding chiral aminopolyol. The current work is addressing the upgrading of the remaining SBP monomer, GalAc, and the modelling of the biorefinery concept to enable economic and Life Cycle Analysis (LCA).

Sugar beet pulp as biomass

2017 ◽  
pp. 29-32 ◽  
Author(s):  
Kazm Eber Özba ◽  
Özen Özboy Özba
Keyword(s):  
Sugar Beet ◽  
Animal Feed ◽  
Sugar Industry ◽  
Low Cost ◽  
Industrial Applications ◽  
Single Cell Protein ◽  
Sugar Beet Pulp ◽  
Raw Material ◽  
Cell Protein ◽  
Beet Pulp

The sugar beet industry produces considerable amounts of organic waste and by-products. Sugar beet pulp (SBP) is the residue that remains after sugar extraction. SBP is a lignocellulosic by-product of the sugar industry and generally used as animal feed at relatively low price. Instead of cattle feeding, SBP can be used as a raw material for industrial applications because it is low-cost and available in large amounts. Biomass is a clean and renewable energy source. The use of SBP for the production of ethanol, methanol, single cell protein, biofuels etc. is economically very attractive. This literature review evaluates the use of SBP as biomass.

scholarly journals A Simple Biorefinery Concept to Produce 2G-Lactic Acid from Sugar Beet Pulp (SBP): A High-Value Target Approach to Valorize a Waste Stream

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2113 ◽  
Author(s):  
Regiane Alves de Oliveira ◽  
Roland Schneider ◽  
Betânia Hoss Lunelli ◽  
Carlos Eduardo Vaz Rossell ◽  
Rubens Maciel Filho ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Sugar Beet ◽  
Animal Feed ◽  
Continuous Fermentation ◽  
Bacillus Coagulans ◽  
Sugar Beet Pulp ◽  
Waste Stream ◽  
Production Chain ◽  
Vast Number ◽  
Beet Pulp

Lactic acid is a high-value molecule with a vast number of applications. Its production in the biorefineries model is a possibility for this sector to aggregate value to its production chain. Thus, this investigation presents a biorefinery model based on the traditional sugar beet industry proposing an approach to produce lactic acid from a waste stream. Sugar beet is used to produce sugar and ethanol, and the remaining pulp is sent to animal feed. Using Bacillus coagulans in a continuous fermentation, 2781.01 g of lactic acid was produced from 3916.91 g of sugars from hydrolyzed sugar beet pulp, with a maximum productivity of 18.06 g L−1h−1. Without interfering in the sugar production, ethanol, or lactic acid, it is also possible to produce pectin and phenolic compounds in the biorefinery. The lactic acid produced was purified by a bipolar membrane electrodialysis and the recovery reached 788.80 g/L with 98% w/w purity.

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