scholarly journals Effect of Experimental Dysglycemia on Under-Carboxylated Osteocalcin Production in Human Primary Osteoblast-Like Cell Cultures

2018 ◽  
Vol 14 (1) ◽  
pp. 11-15
Author(s):  
A Kacso
Keyword(s):  
Cell Cultures ◽  
Primary Osteoblast ◽  
Osteocalcin Production ◽  
Human Primary Osteoblast

scholarly journals Osteocalcin Production in Primary Osteoblast Cultures Derived from Normal and Hyp Mice

Endocrinology ◽  
1998 ◽  
Vol 139 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Thomas O. Carpenter ◽  
Kathleen C. Moltz ◽  
Bruce Ellis ◽  
Monica Andreoli ◽  
Thomas L. McCarthy ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Cultured Cells ◽  
Primary Cultures ◽  
Specific Effect ◽  
Continuous Exposure ◽  
Primary Osteoblast ◽  
Characteristic Features ◽  
Species Specific ◽  
Osteocalcin Production

Abstract Rickets and osteomalacia are characteristic features of the Hyp mouse model of human X-linked hypophosphatemia. Hyp mice demonstrate elevated circulating osteocalcin levels, as well as altered regulation of osteocalcin by 1,25(OH)2D3. Whether this osteocalcin abnormality is intrinsic to the osteoblast, or mediated by the in vivo milieu, has not been established. We therefore characterized osteocalcin production and its regulation by 1,25(OH)2D3 in primary cultures of murine osteoblasts and examined osteocalcin and its messenger RNA in response to 1,25(OH)2D3 in cultures of Hyp mouse-derived osteoblasts. Cell viability and osteocalcin production are optimal when murine cells are harvested within 36 h of age. Murine primary osteoblast cultures mineralize and produce osteocalcin in a maturation-dependent fashion (as demonstrated in other species), and continuous exposure to 1,25(OH)2D3, beginning at day 9 of culture, inhibits osteoblast differentiation and osteocalcin production and prevents mineralization of the culture. However, in contrast to other species, exposure to 1,25(OH)2D3, added later (days 17–25) in culture, does not stimulate osteocalcin but arrests osteocalcin production at current levels. Ambient media levels of osteocalcin were no different in cultures from Hyp mice and their normal litter mates, and the down-regulatory response to 1,25(OH)2D3 was comparable in cultures from normal and Hyp mice. Furthermore, expression of osteocalcin messenger RNA in murine cultures is reduced with exposure to 1,25(OH)2D3, and there is no difference between normal and Hyp cultures in this response. Thus, primary murine osteoblasts manifest a species-specific effect of 1,25(OH)2D3 on osteocalcin production. Furthermore, the increased serum osteocalcin production seen in intact Hyp mice, and the altered response to 1,25(OH)2D3 in Hyp mice, are not observed in osteoblast cultures derived from the mutant strain. These data indicate that abnormalities of osteocalcin described in intact Hyp mice require factors other than those present in cultured cells.

Characterization of human primary osteoblast response on bioactive glass (BaG 13–93)- coated poly-L,DL-lactide (SR-PLA70) surfacein vitro

2006 ◽  
Vol 78B (1) ◽  
pp. 97-104 ◽  
Author(s):  
P. Ruuttila ◽  
H. Niiranen ◽  
M. Kellomäki ◽  
P. Törmälä ◽  
Y. T. Konttinen ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Primary Osteoblast ◽  
Human Primary Osteoblast

Effect of 1α,25(OH)2-Vitamin D3on TNFα-Mediated Apoptosis of Human Primary Osteoblast-Like Cellsin Vitro

1999 ◽  
Vol 31 (12) ◽  
pp. 653-656 ◽  
Author(s):  
E. Pascher ◽  
A. Perniok ◽  
A. Becker ◽  
J. Feldkamp
Keyword(s):  
Primary Osteoblast ◽  
Human Primary Osteoblast

L-Carnitine facilitates mitochondrial activity and osteogenic differentiation in human primary osteoblast culture

2016 ◽  
Author(s):  
Fernanda Vacante ◽  
Anna Montesano ◽  
Alice Spinello ◽  
Simona Bolamperti ◽  
Pamela Senesi ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Mitochondrial Activity ◽  
Primary Osteoblast ◽  
Osteoblast Culture ◽  
Human Primary Osteoblast

Mineralized human primary osteoblast matrices as a model system to analyse interactions of prostate cancer cells with the bone microenvironment

Biomaterials ◽  
2010 ◽  
Vol 31 (31) ◽  
pp. 7928-7936 ◽  
Author(s):  
Johannes C. Reichert ◽  
Verena M.C. Quent ◽  
Leslie J. Burke ◽  
Scott H. Stansfield ◽  
Judith A. Clements ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Model System ◽  
Bone Microenvironment ◽  
Prostate Cancer Cells ◽  
Primary Osteoblast ◽  
Human Primary Osteoblast

Casein phosphopeptides promote calcium uptake and modulate the differentiation pathway in human primary osteoblast-like cells

Peptides ◽  
2009 ◽  
Vol 30 (12) ◽  
pp. 2233-2241 ◽  
Author(s):  
Bianca Maria Donida ◽  
Emanuela Mrak ◽  
Claudia Gravaghi ◽  
Isabella Villa ◽  
Stefania Cosentino ◽  
...  
Keyword(s):  
Calcium Uptake ◽  
Primary Osteoblast ◽  
Casein Phosphopeptides ◽  
Human Primary Osteoblast

scholarly journals The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157874 ◽  
Author(s):  
Nezha Ahmad Agha ◽  
Regine Willumeit-Römer ◽  
Daniel Laipple ◽  
Bérengère Luthringer ◽  
Frank Feyerabend
Keyword(s):  
Direct Contact ◽  
Osteoblast Cells ◽  
Primary Osteoblast ◽  
Human Primary Osteoblast

scholarly journals Biomimetic Mineralization on 3D Printed PLA Scaffolds: On the Response of Human Primary Osteoblasts Spheroids and In Vivo Implantation

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 74
Author(s):  
Marianna O. C. Maia-Pinto ◽  
Ana Carolina B. Brochado ◽  
Bruna Nunes Teixeira ◽  
Suelen C. Sartoretto ◽  
Marcelo J. Uzeda ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Growth Factors ◽  
Growth Factor ◽  
Biological Response ◽  
Primary Osteoblast ◽  
Rat Calvaria ◽  
3D Printed ◽  
Human Primary Osteoblast

This study aimed to assess the response of 3D printed polylactic acid (PLA) scaffolds biomimetically coated with apatite on human primary osteoblast (HOb) spheroids and evaluate the biological response to its association with Bone Morphogenetic Protein 2 (rhBMP-2) in rat calvaria. PLA scaffolds were produced via 3D printing, soaked in simulated body fluid (SBF) solution to promote apatite deposition, and characterized by physical-chemical, morphological, and mechanical properties. PLA-CaP scaffolds with interconnected porous and mechanical properties suitable for bone repairing were produced with reproducibility. The in vitro biological response was assessed with human primary osteoblast spheroids. Increased cell adhesion and the rise of in vitro release of growth factors (Platelet-Derived Growth Factor (PDGF), Basic Fibroblast Growth Factor (bFGF), Vascular Endothelial Growth Factor (VEGF) was observed for PLA-CaP scaffolds, when pre-treated with fetal bovine serum (FBS). This pre-treatment with FBS was done in a way to enhance the adsorption of serum proteins, increasing the number of bioactive sites on the surface of scaffolds, and to partially mimic in vivo interactions. The in vivo analysis was conducted through the implantation of 3D printed PLA scaffolds either alone, coated with apatite (PLA-CaP) or PLA-CaP loaded with rhBMP-2 on critical-sized defects (8 mm) of rat calvaria. PLA-CaP+rhBMP2 presented higher values of newly formed bone (NFB) than other groups at all in vivo experimental periods (p < 0.05), attaining 44.85% of NFB after six months. These findings indicated two new potential candidates as alternatives to autogenous bone grafts for long-term treatment: (i) 3D-printed PLA-CaP scaffold associated with spheroids, since it can reduce the time of repair in situ by expression of biomolecules and growth factors; and (ii) 3D-printed PLA-CaP functionalized rhBMP2 scaffold, a biocompatible, bioactive biomaterial, with osteoconductivity and osteoinductivity.

scholarly journals BMP-4 of Xenopus laevis stimulates differentiation of human primary osteoblast-like cells

2001 ◽  
Vol 83-B (1) ◽  
pp. 144-147
Author(s):  
U. Mayr-Wohlfart ◽  
S. Kessler ◽  
W. Puhl ◽  
K. P. Günther ◽  
W. Knöchel
Keyword(s):  
Xenopus Laevis ◽  
Primary Osteoblast ◽  
Human Primary Osteoblast
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