Hydrolytic Degradation Study of  Lansoprazole, Identification, Isolation  and Characterisation of Base  Degradation Product

Abstract A stability-indicating micellar liquid chromatography (MLC) method was developed and validated for the assay of floctafenine (FLF) in the presence of its degradation product and main metabolite, floctafenic acid (FLA). The analysis was carried out on a CLC Shim-Pack octyl silane (C8) column (150 × 4.6 mm id, 5 μm particle size) using a micellar mobile phase consisting of 0.15 M sodium dodecyl sulfate, 10% n-propanol, and 0.3% triethylamine in 0.02 M orthophosphoric acid (pH = 3). The mobile phase was pumped at a flow rate of 1.0 mL/min with UV detection at 360 nm. The method showed good linearity for FLF and FLA over the concentration ranges of 0.5–25.0 and 0.4–10.0 μg/mL, with LODs of 0.16 and 0.12 μg/mL, respectively. The developed method was successfully applied to the determination of FLF in commercial dispersible tablets, with mean recovery of 98.87 ± 1.37%. Also, the proposed method was specific for the analysis of FLF in presence of the co-formulated drug thiocolchicoside in laboratory-prepared tablets, with mean recovery of 100.50 ± 1.07%. Statistical comparison of the results obtained by the proposed MLC method with those obtained by a comparison method showed good agreement. Moreover, the method was extended to study the degradation behavior of FLF under different International Conference on Harmonization recommended conditions such as alkaline, acidic, oxidative, thermal, and photolytic. The method was further applied for direct determination of FLA as the main metabolite of FLF in human plasma without prior extraction steps, with mean recovery of 110.50 ± 6.5%.

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Polymer Membranes Sonocoated and Electrosprayed with Nano-Hydroxyapatite for Periodontal Tissues Regeneration

Nanomaterials ◽

10.3390/nano9111625 ◽

2019 ◽

Vol 9 (11) ◽

pp. 1625 ◽

Cited By ~ 7

Author(s):

Higuchi ◽

Fortunato ◽

Woźniak ◽

Chodara ◽

Domaschke ◽

...

Keyword(s):

Bone Regeneration ◽

Calcium Release ◽

Healing Process ◽

Hydrolytic Degradation ◽

Clinical Problem ◽

Degradation Study ◽

Periodontal Tissues ◽

Inflammatory Processes ◽

Cellular Metabolic Activity ◽

Gradual Release

Diseases of periodontal tissues are a considerable clinical problem, connected with inflammatory processes and bone loss. The healing process often requires reconstruction of lost bone in the periodontal area. For that purpose, various membranes are used to prevent ingrowth of epithelium in the tissue defect and enhance bone regeneration. Currently-used membranes are mainly non-resorbable or are derived from animal tissues. Thus, there is an urgent need for non-animal-derived bioresorbable membranes with tuned resorption rates and porosity optimized for the circulation of body nutrients. We demonstrate membranes produced by the electrospinning of biodegradable polymers (PDLLA/PLGA) coated with nanohydroxyapatite (nHA). The nHA coating was made using two methods: sonocoating and electrospraying of nHA suspensions. In a simulated degradation study, for electrosprayed membranes, short-term calcium release was observed, followed by hydrolytic degradation. Sonocoating produced a well-adhering nHA layer with full coverage of the fibers. The layer slowed the polymer degradation and increased the membrane wettability. Due to gradual release of calcium ions the degradation-associated acidity of the polymer was neutralized. The sonocoated membranes exhibited good cellular metabolic activity responses against MG-63 and BJ cells. The collected results suggest their potential use in Guided Tissue Regeneration (GTR) and Guided Bone Regeneration (GBR) periodontal procedures.

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Studying Microwave-Assisted Hydrolytic Degradation of Colchicine using RP-Chromatographic Methods: In Silico ADME/Tox Profile and Molecular Docking

10.21203/rs.2.16552/v1 ◽

2019 ◽

Author(s):

nada abdelwahab ◽

hossam mokhtar ◽

asmaa aboulmaged

Keyword(s):

Degradation Product ◽

In Silico ◽

Oral Absorption ◽

Hydrolytic Degradation ◽

High Sensitivity ◽

Flash Chromatography ◽

Stability Indicating ◽

Microwave Assisted ◽

Chromatographic Methods ◽

Validation Parameters

Abstract Colchicine, is a natural amide containing anti-gout treatment with versatile applications. Microwave assisted hydrolytic degradation is a newly alternative method thought to be more promising than traditional procedures of heating. It is an ecofriendly method that has more reproducible results due to the control of parameters. From this point, carrying on hydrolytic degradation of colchicine was tested for the first time under acidic conditions with the aided of microwave. The drug was hydrolyzed with the formation of deacylated analogue. Isolation of the resulted degradate was carried out using flash chromatography, the isolated one was elucidated based on 1 H NMR data. Moreover, the results of human pharmacokinetic predictions conducted from in silico data showed that colchicine had higher blood brain barrier (BBB), plasma protein binding, and oral absorption than its deacylated derivative. The study was also extended to forecast the binding of colchicine and its degradate to the target protein. Furthermore, two stability indicating chromatographic methods were developed for quantification of the drug and its degradation product with high sensitivity. The first method was RP-TLC densitometric method that based on using a solvent mixture of water: methanol: diethylamine (70: 30: 15, by volume). The second one was RP-HPLC at which a mixture of water (containing 0.02% diethyl amine): methanol: acetonitrile (50: 20: 30, by volume) was the used mobile phase. Validation parameters were calculated according to ICH recommendations and all were within the acceptable limits. These methods were used for determination of colchicine in its available tablets. They are the first developed stability indicating methods for analysis of colchicine and its degradation product.

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Hydrolytic Degradation Study of Dabigatran Etexilate Mesylate: Isolation and Structural Elucidation of New Degradants

Analytical Chemistry Letters ◽

10.1080/22297928.2018.1449664 ◽

2018 ◽

Vol 8 (3) ◽

pp. 277-286

Author(s):

V.V.S.R.N. Anji Karun Mutha ◽

B.V. Narasimha Raju Katari ◽

Muralidharan Kaliyaperumal ◽

Chidananda Swamy Rumalla ◽

Raghu Babu Korupolu ◽

...

Keyword(s):

Dabigatran Etexilate ◽

Hydrolytic Degradation ◽

Structural Elucidation ◽

Degradation Study

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Further Studies on the Colorimetric Determination of Ronidazole in Feeds

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/61.6.1523 ◽

1978 ◽

Vol 61 (6) ◽

pp. 1523-1526

Author(s):

David W Fink ◽

James V Pivnichny ◽

Jung-Sook K Shim ◽

John W Tolan

Keyword(s):

Sample Preparation ◽

Degradation Product ◽

Colorimetric Method ◽

Hydrolytic Degradation ◽

Colorimetric Determination ◽

Analytical Technique ◽

Per Se ◽

The Stability ◽

Medicated Feeds

Abstract A previously published colorimetric method for determining ronidazole, (l-methyl-5-nitroimidazoI- 2-yl) methyl carbamate, can be used as an analytical technique for measuring the stability of this drug in medicated feeds. Although the color reaction per se is not selective, elution profiles of ronidazole and its demonstrated hydrolytic degradation product, l-methyl-2-hydroxymethyl- 5-nitroimidazoIe, show that the chromatographic separation used in the sample preparation efficiently isolates the drug from the degradation product. No interference was found in feeds containing 0.010% ronidazole and up to 0.010% degradation product.

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Stability-indicating spectrophotometric methods for determination of the anticoagulant drug apixaban in the presence of its hydrolytic degradation product

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy ◽

10.1016/j.saa.2016.01.029 ◽

2016 ◽

Vol 159 ◽

pp. 13-20 ◽

Cited By ~ 5

Author(s):

Mahmoud A. Tantawy ◽

Nariman A. El-Ragehy ◽

Nagiba Y. Hassan ◽

Mohamed Abdelkawy

Keyword(s):

Degradation Product ◽

Hydrolytic Degradation ◽

Stability Indicating ◽

Spectrophotometric Methods ◽

Anticoagulant Drug

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GRISEOVIRIDIN: THE C6 FRAGMENT

Canadian Journal of Chemistry ◽

10.1139/v60-133 ◽

1960 ◽

Vol 38 (6) ◽

pp. 950-957 ◽

Cited By ~ 6

Author(s):

P. De Mayo ◽

A. Stoessl

Keyword(s):

Degradation Product ◽

Hydrolytic Degradation ◽

Sulphur Atom ◽

Structural Environment ◽

Part Structure

The structure of a hydrolytic degradation product from griseoviridin, isolated as the 2,4-dinitrophenylhydrazone, has been shown, by synthesis, to be IV. A by-product of the synthesis was the stereoisomeric 2,4-dinitrophenylhydrazone. This establishes IX as a part structure of griseoviridin. Evidence is provided that the previously proposed structural environment of the sulphur atom is incorrect, and that the sulphur may, in fact, be attached to the C6 fragment.

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