The Relationship between Nigerian Bonnylight Crude Oil-Induced Hypoglycaemia and Endogenous Serum Insulin Concentration in Male Wistar  Rats: The Role of Antioxidant Vitamins C and E

AbstractInsulin is involved in coronary heart disease through diabetes and metabolic syndrome. A great deal is known about insulin and its correlates, as well as factors related to changes in insulin. However, few studies consider the broad variety of correlates simultaneously. Therefore, the aims of the present study were to characterize the main factors of biological variation affecting serum insulin concentration and to establish reference limits of insulinemia in a presumably healthy French population. Insulin was measured using a microparticular enzymatic immunoassay. A total of 646 subjects aged 11–58 years from the STANISLAS cohort and divided into four groups of 162 males, 157 females, 163 boys and 164 girls, were included in the statistical analyses. In the whole population, serum insulin concentration varied from 0.80 to 54.60 µU/ml. Significant factors affecting insulin were age, gender, body mass index and glucose, in addition to alanine aminotransferase and high-density lipoprotein cholesterol in men, triglycerides and oral contraceptive use in women, and alkaline phosphatase in girls.In summary, we presented biological correlates of insulin in both healthy French male and female adults and children/adolescents and determined reference limits for insulin for each group. These results will contribute to a better interpretation of insulin data in further studies and laboratory investigations.

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Preperitoneal fat thickness in childhood obesity: Association with serum insulin concentration

Pediatrics International ◽

10.1046/j.1442-200x.2000.01197.x ◽

2000 ◽

Vol 42 (2) ◽

pp. 155-159 ◽

Cited By ~ 20

Author(s):

Akiho Tamura ◽

Tetsuo Mori ◽

Yoji Hara ◽

Atsushi Komiyama

Keyword(s):

Childhood Obesity ◽

Serum Insulin ◽

Insulin Concentration ◽

Serum Insulin Concentration ◽

Fat Thickness

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Acetylcholine and muscarinic receptor function in cerebral cortex of diabetic young and old male Wistar rats and the role of muscarinic receptors in calcium release from pancreatic islets

Biogerontology ◽

10.1007/s10522-009-9237-z ◽

2009 ◽

Vol 11 (2) ◽

pp. 151-166 ◽

Cited By ~ 3

Author(s):

Balakrishnan Savitha ◽

Binoy Joseph ◽

T. Peeyush Kumar ◽

C. S. Paulose

Keyword(s):

Cerebral Cortex ◽

Pancreatic Islets ◽

Muscarinic Receptor ◽

Muscarinic Receptors ◽

Wistar Rats ◽

Calcium Release ◽

Receptor Function ◽

Male Wistar Rats

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Hormonal assays following oral administration of bonny light crude oil on male wistar rats

Journal of Experimental and Clinical Anatomy ◽

10.4103/1596-2393.127960 ◽

2013 ◽

Vol 12 (2) ◽

pp. 53

Author(s):

VictorA Fischer ◽

ChristieE Fischer ◽

Mfon Akpaso ◽

OladapoA Ashiru

Keyword(s):

Oral Administration ◽

Crude Oil ◽

Wistar Rats ◽

Light Crude Oil ◽

Male Wistar Rats

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Glucose-induced changes in pancreatic islet blood flow mediated by central nervous system

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1986.251.6.e644 ◽

1986 ◽

Vol 251 (6) ◽

pp. E644-E647 ◽

Cited By ~ 14

Author(s):

L. Jansson ◽

C. Hellerstrom

Keyword(s):

Central Nervous System ◽

Blood Flow ◽

Nervous System ◽

Glucose Concentration ◽

Serum Insulin ◽

Serum Glucose ◽

Insulin Concentration ◽

Similar Amount ◽

Serum Insulin Concentration ◽

Islet Blood Flow

Earlier experiments with the microsphere technique suggested that a heightened serum glucose concentration consistently leads to an increase in islet blood flow (IBF). Several lines of evidence suggest that this glucose-sensitive control mechanism is located at an extrapancreatic site. The purpose of this study was to define the possible role of the central nervous system in such a mechanism. D-glucose, L-glucose, 3-O-methylglucose, or saline were therefore infused into the carotid artery, each at a dose of 1 mg X kg body wt-1 X min-1 for 3 min, and the pancreatic and islet blood flows were measured. None of these substances affected the systemic serum glucose level. The intracarotid infusion of D-glucose, however, caused a rapid increase in both the serum insulin concentration and IBF. The blood flow to the whole pancreas nevertheless remained unchanged, indicating a redistribution of flow within the gland. Carotid infusion of the other test substances or a similar amount of D-glucose given in a femoral vein did not affect these parameters. Both the increase in serum insulin concentration and the increase in IBF caused by D-glucose could be abolished by vagotomy or administration of atropine. When the systemic blood glucose concentration was increased by intraperitoneal glucose administration (2 g/kg body wt), vagotomy blocked the increase in islet blood flow but not the concomitant insulin release. These observations suggest that the glucose-induced increase in IBF is mediated by vagal cholinergic influences.

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Differences in the Role of HDACs 4 and 5 in the Modulation of Processes Regulating MAFbx and MuRF1 Expression during Muscle Unloading

International Journal of Molecular Sciences ◽

10.3390/ijms21134815 ◽

2020 ◽

Vol 21 (13) ◽

pp. 4815 ◽

Cited By ~ 1

Author(s):

Ekaterina P. Mochalova ◽

Svetlana P. Belova ◽

Tatiana Y. Kostrominova ◽

Boris S. Shenkman ◽

Tatiana L. Nemirovskaya

Keyword(s):

Skeletal Muscle ◽

Mrna Expression ◽

Histone Deacetylases ◽

Wistar Rats ◽

Hindlimb Suspension ◽

Skeletal Muscle Atrophy ◽

E3 Ligases ◽

Male Wistar Rats ◽

Muscle Unloading

Unloading leads to skeletal muscle atrophy via the upregulation of MuRF-1 and MAFbx E3-ligases expression. Reportedly, histone deacetylases (HDACs) 4 and 5 may regulate the expression of MuRF1 and MAFbx. To examine the HDAC-dependent mechanisms involved in the control of E3-ubiquitin ligases expression at the early stages of muscle unloading we used HDACs 4 and 5 inhibitor LMK-235 and HDAC 4 inhibitor Tasqinimod (Tq). Male Wistar rats were divided into four groups (eight rats per group): nontreated control (C), three days of unloading/hindlimb suspension (HS) and three days HS with HDACs inhibitor LMK-235 (HSLMK) or Tq (HSTq). Treatment with LMK-235 diminished unloading-induced of MAFbx, myogenin (MYOG), ubiquitin and calpain-1 mRNA expression (p < 0.05). Tq administration had no effect on the expression of E3-ligases. The mRNA expression of MuRF1 and MAFbx was significantly increased in both HS and HSTq groups (1.5 and 4.0 folds, respectively; p < 0.05) when compared with the C group. It is concluded that during three days of muscle unloading: (1) the HDACs 4 and 5 participate in the regulation of MAFbx expression as well as the expression of MYOG, ubiquitin and calpain-1; (2) the inhibition of HDAC 4 has no effect on MAFbx expression. Therefore, HDAC 5 is perhaps more important for the regulation of MAFbx expression than HDAC 4.

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