scholarly journals Assessment of Domestic Water Sources Safety: Application of the Micro Biological Survey Method and Microbiological Profile of the Contaminating Bacteria

2021 ◽  
Vol 13 (05) ◽  
pp. 350-361
Author(s):  
Sali Ben Béchir Adogaye ◽  
Mabvouna Biguioh Rodrigue ◽  
Nkamedjie Pete Patrick Martial ◽  
Andrillene Laure Deutou Wondeu ◽  
Sanou Sobze Martin ◽  
...  
Keyword(s):  
Water Sources ◽  
Survey Method ◽  
Domestic Water ◽  
Microbiological Profile ◽  
Biological Survey ◽  
Safety Application

scholarly journals Microbiological quality and genotoxicity of domestic water sources: A combined approach using Micro Biological Survey method and mutagenesis assay (micronucleus test) in root tips of Vicia faba in the West region of Cameroon

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0245379
Author(s):  
Rodrigue Biguioh Mabvouna ◽  
Sali Ben Béchir Adogaye ◽  
Patrick Martial Pete Nkamedjie ◽  
Andrillene Laure Deutou Wondeu ◽  
Martin Sanou Sobze ◽  
...  
Keyword(s):  
Water Samples ◽  
Microbiological Quality ◽  
Water Sources ◽  
Survey Method ◽  
Root Tips ◽  
Domestic Water ◽  
Biological Survey ◽  
West Region ◽  
Quality Of Water

At least 2.1 billion people around the world use contaminated drinking water, causing 485,000 diarrheal deaths each year, mostly among children under 5 years old. A study conducted 10 years ago in Bafoussam (West Cameroon) recorded concentrations of bacteria among surface and groundwater. High levels of bicarbonates, phosphates, chlorides and suspended matters were also found. The aim of this study was to assess the microbiological and chemical qualities of domestic water sources in 5 localities of the West region of Cameroon. Water samples from 22 water sources (wells, springs, water drilling and river) were aseptically collected in plastic bottles and transferred in 50 ml sterile tubes. For chlorinated water sources, 1 ml of Thiosulfate was added to the water sample; immediately placed in an ice box and transported to the laboratory for analysis. Water temperature and pH were measured on site. The microbiological quality of water was determined by testing Total Coliforms (TC) using the Micro Biological Survey method. 1 ml of each water sample was inoculated in the MBS vial initially rehydrated with 10 ml of sterile distilled water. The initial color of the vials is red. Color changes were monitored at three different time intervals (12h, 19h and 24h), corresponding to three levels of contamination. The chemical quality of water was assessed using micronucleus (MN) test in selected Vicia faba seeds secondary root tips permanently mounted in Dibutylphthalate Polystyrene Xylene mountant for histology after 72 hours of direct exposition in water samples and in dark. The mitotic indices and MN frequencies were evaluated in 10 root tips per site analysing 5000 cells per tip. Statistical analyses were done using Stata IC/15.0 software. The Student t-test was used for mean comparison and the significance level was set at 1%. The majority of samples were collected from wells (63.6%). The mean water pH ranged from 5.5 to 8.3 and the temperature varied from 23 to 26°C. A very high concentration of TC [>103 CFU/ml] was found in 8 (36.4%) samples. 10 (45.5%) and 2 (9.1%) samples turned yellow at 19 and 24 hours respectively after incubation corresponding to TC concentration of [10<x<103 CFU/ml] and [1<x< 10 CFU/ml]. The MN frequency was higher (P ≤ 0.01) compared to the negative control in 9 (40.9%) water samples indicating significant genotoxic effects of these water sources. This study highlighted the poor quality of domestic water sources in West region of Cameroon and the need to conduct regular monitoring of drinking water sources. Community capacity building on water treatment methods, including good wastes management should be implemented to help improve water quality.

scholarly journals Field application of the Micro Biological Survey method for the assessment of the microbiological safety of different water sources in Tanzania

2018 ◽  
Vol 9 (3) ◽  
Author(s):  
Matteo Gionfriddo ◽  
Beatrice Nicolosi ◽  
Lorenza Murgia ◽  
Alyexandra Arienzo ◽  
Laura De Gara ◽  
...  
Keyword(s):  
Developing Countries ◽  
Tap Water ◽  
Water Sources ◽  
Field Application ◽  
Alternative Methods ◽  
Survey Method ◽  
Water Safety ◽  
Biological Survey ◽  
Enteric Infections ◽  
Dug Wells

Access to safe water is stated within human rights as essential for life, as water can be a source of severe enteric infections threatening human health, in particular children from Developing Countries. Along with reference methods, need is pressing for alternative methods to flank reference ones to improve water safety on-site monitoring and in the absence of scientific facilities or even electricity supply. The Micro Biological Survey (MBS) method has already been successfully applied to water safety assessment in Developing Countries. A total of 18 water samples were collected from different sources (rivers, dug wells, tap water) within the Rukwa Region, Tanzania, and underwent analysis for Total Coliforms following the MBS method. Globally, rivers showed more frequently contamination, followed by dug wells, tap water and tanks. Results demonstrate the need for continuous monitoring of water sources, even in difficult frameworks lacking electric supply, to help improve control over water quality, possibly using alternative methods to simplify existing protocols.

scholarly journals Field application of the Micro Biological Survey method for the assessment of the microbiological safety of different water sources in Horn of Africa and the evaluation of the effectiveness of Moringa oleifera in drinking water purification

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Francesca Losito ◽  
Alyexandra Arienzo ◽  
Daniela Somma ◽  
Lorenza Murgia ◽  
Ottavia Stalio ◽  
...  
Keyword(s):  
Developing Countries ◽  
Drinking Water ◽  
Bacterial Load ◽  
Water Sources ◽  
Survey Method ◽  
Horn Of Africa ◽  
Water Safety ◽  
Microbiological Safety ◽  
Biological Survey ◽  
Different Water Sources

Water monitoring requires expensive instrumentations and skilled technicians. In developing Countries as Africa, the severe economic restrictions and lack of technology make water safety monitoring approaches applied in developed Countries, still not sustainable. The need to develop new methods that are suitable, affordable, and sustainable in the African context is urgent. The simple, economic and rapid Micro Biological Survey (MBS) method does not require an equipped laboratory nor special instruments and skilled technicians, but it can be very useful for routine water analysis. The aim of this work was the application of the MBS method to evaluate the microbiological safety of different water sources and the effectiveness of different drinking water treatments in the Horn of Africa. The obtained results have proved that this method could be very helpful to monitor water safety before and after various purification treatments, with the aim to control waterborne diseases especially in developing Countries, whose population is the most exposed to these diseases. In addition, it has been proved that <em>Moringa</em> <em>oleifera</em> water treatment is ineffective in decreasing bacterial load of Eritrea water samples.

scholarly journals Microbiological quality of water sources in the West region of Cameroon: quantitative detection of total coliforms using Micro Biological Survey method

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Rodrigue Mabvouna Biguioh ◽  
Sali Ben Béchir Adogaye ◽  
Patrick Martial Nkamedjie Pete ◽  
Martin Sanou Sobze ◽  
Jean Blaise Kemogne ◽  
...  
Keyword(s):  
Microbiological Quality ◽  
Water Sources ◽  
Survey Method ◽  
Quantitative Detection ◽  
The West ◽  
Total Coliforms ◽  
Biological Survey ◽  
West Region ◽  
Quality Of Water

scholarly journals Microbiological Quality of Ready-to-Eat Leafy Green Salads during Shelf-Life and Home-Refrigeration

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1421
Author(s):  
Alyexandra Arienzo ◽  
Lorenza Murgia ◽  
Ilaria Fraudentali ◽  
Valentina Gallo ◽  
Riccardo Angelini ◽  
...  
Keyword(s):  
Shelf Life ◽  
Small And Medium Enterprises ◽  
Microbiological Quality ◽  
Survey Method ◽  
National Guidelines ◽  
Salmonella Spp ◽  
Expiry Date ◽  
Mesophilic Bacteria ◽  
Biological Survey

The market of ready-to-eat leafy green salads is experiencing a noticeable growth in Europe. Since they are intended to be consumed without additional treatments, these ready-to-eat products are associated with a high microbiological risk. The aim of this work was to evaluate the microbiological quality and safety of ready-to-eat leafy green salads sold in widespread supermarket chains in Lazio, Italy, on the packaging date during shelf-life and during home-refrigeration. The study also aimed to determine the differences between low-, medium-, and high-cost products. Salmonella spp. and L. monocytogenes were chosen as safety indicators as specified by European regulations while total aerobic mesophilic bacteria and Escherichia coli were chosen as quality indicators as suggested by national guidelines. Analyses were performed following the ISO standards and in parallel for the evaluation of total aerobic mesophilic bacteria, with an alternative colorimetric system, the Micro Biological Survey method, in order to propose a simple, affordable and accurate alternative for testing the microbiological quality of products, especially suitable for small and medium enterprises and on-site analyses. The study revealed high, unsatisfactory, total bacterial loads in all analyzed samples on the packaging date and expiry date and a very high prevalence of Salmonella spp. (67%) regardless of the selected varieties and cost categories; L. monocytogenes was not recovered aligning with the results obtained in other studies.

scholarly journals Xenic Cultivation and Genotyping of Pathogenic Free-Living Amoeba from Public Water Supply Sources in Uganda

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Celsus Sente ◽  
Joseph Erume ◽  
Irene Naigaga ◽  
Benigna Gabriela Namara ◽  
Julius Mulindwa ◽  
...  
Keyword(s):  
Water Treatment ◽  
Water Supply ◽  
Protected Area ◽  
Water Sources ◽  
Public Water Supply ◽  
Contaminated Water ◽  
Domestic Water ◽  
Free Living ◽  
Water Contaminants ◽  
Free Living Amoeba

Studies on waterborne parasites from natural environment and domestic water sources in Uganda are very scarce and unpublished. Water dwelling free-living amoebae (FLA) of the genus Acanthamoeba, Hartmannella, and Naegleria are often responsible for causing morbidities and mortalities in individuals with recent contact with contaminated water, but their presence in Uganda’s public water supply sources is not known. We cultivated and genotyped FLA from natural and domestic water from Queen Elizabeth Protected Area (QEPA) and Kampala (KLA). The cultivated parasites were observed microscopically and recorded. The overall prevalence of FLA in QEPA (Acanthamoeba spp., 35%; Hartmannella spp., 18.9%; Naegleria spp., 13.5%) and KLA (Acanthamoeba spp., 28.3%; Naegleria spp., 16.6%; Hartmannella spp., 23.1%) were not significantly different. The highest prevalence across water sources in QEPA and KLA was observed for Acanthamoeba spp., followed by Hartmannella spp., and Naegleria spp. Overall FLA mean (±SE) and mean (±SE) across water sources were highest for Acanthamoeba spp. compared to other FLA but were not statistically significant (p > 0.05). Analysis of the FLA sequences produced 1 Cercomonas, 1 Nuclearia, 1 Bodomorpha, 2 Hartmannella, 5 Echinamoeba, and 7 Acanthamoeba partial sequences, indicating a muliplicity of water contaminants that need to be controlled by proper water treatment.

scholarly journals Isolation and molecular characterization of Acanthamoeba genotypes in recreational and domestic water sources from Jamaica, West Indies

2015 ◽  
Vol 13 (3) ◽  
pp. 909-919 ◽  
Author(s):  
Cheridah D. Todd ◽  
María Reyes-Batlle ◽  
José E. Piñero ◽  
Enrique Martínez-Carretero ◽  
Basilio Valladares ◽  
...  
Keyword(s):  
Water Sources ◽  
Skin Infections ◽  
Domestic Water ◽  
Free Living ◽  
Water Contact ◽  
First Report ◽  
The Caribbean ◽  
Polymerase Chain ◽  
Neurological Infections

Free living amoebae (FLA) are amphizoic protozoa that are ubiquitous in nature. Infection with FLA may result in neurological, ocular and skin infections. Exposure to Acanthamoeba occurs frequently through water contact and knowledge of the presence of the organisms in water sources is important in understanding transmission dynamics. The distribution of Acanthamoeba was studied in recreational and domestic water samples collected from across Jamaica. Morphological assessment and polymerase chain reaction revealed Acanthamoeba spp. isolates in 50.6% (42/83) and 17.3% (14/81) of recreational and domestic water, respectively. Sequencing of the DF3 region of the 18S rDNA resulted in the identification of genotypes T3, T4, T5, T10 and T11 corresponding to Acanthamoeba spp: A. griffini, A. triangularis, A. lenticulata, A. culbertsoni and A. hatchetti. Moreover, T4 was the most frequently isolated genotype in both recreational and domestic water. Thermotolerance and osmotolerance assays indicated that most isolates were potentially pathogenic. This is the first report of T3 and T10 genotypes in the Caribbean and the first report of these Acanthamoeba spp. in Jamaican waters. The study shows that there is potential risk of infection to contact wearers who practise poor lens care. Further, Acanthamoeba should be considered as a cause of neurological infections in Jamaica.

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