Prolonged Storage of Red Blood Cells for Transfusion in Citrate Phosphate Dextrose Adenine-1 Affects Their Viability

Objective In stored red blood cells (RBCs), which are used in diseases (e.g., acute blood loss and leukaemia), storage lesions arise by oxidative stress and other factors over time. This study investigated the protective effects of resveratrol and serotonin on stored RBCs. Methods Blood from each donor (n = 10) was placed in different bags containing 70 mL of citrate phosphate dextrose (total volume: 500 mL) and divided into three groups (n = 30): control, 60 µg/mL resveratrol, and 60 µg/mL serotonin. Malondialdehyde (MDA) and glutathione (GSH) levels, activity of glutathione peroxidase (GSH-Px), catalase, and carbonic anhydrase (CA), and susceptibility to oxidation in RBCs, and pH in whole blood were measured at baseline and on days 7, 14, 21, and 28. Results MDA levels and susceptibility to oxidation were increased in all three groups time-dependently, but this increase was greater in the serotonin group than in the other groups. Activity of GSH-Px, CAT, and CA, as well as GSH levels, were decreased in the control and serotonin groups time-dependently, but were significantly preserved in the resveratrol group. The pH was decreased in all groups time-dependently. Conclusion Our study shows that resveratrol attenuates susceptibility to oxidation of RBCs and protects their antioxidant capacity, and partially preserves CA activity time-dependently.

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Mesenchymal stromal cells can be applied to red blood cells storage as a kind of cellular additive

Bioscience Reports ◽

10.1042/bsr20170676 ◽

2017 ◽

Vol 37 (5) ◽

Cited By ~ 1

Author(s):

Yaozhen Chen ◽

Jing Zhang ◽

Shunli Gu ◽

Dandan Yin ◽

Qunxing An ◽

...

Keyword(s):

Red Blood Cells ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Blood Cells ◽

Storage Duration ◽

Hematopoietic Stem ◽

Diminished Capacity ◽

Blood Banks ◽

Citrate Phosphate

During storage in blood banks, red blood cells (RBCs) undergo the mechanical and metabolic damage, which may lead to the diminished capacity to deliver oxygen. At high altitude regions, the above-mentioned damage may get worse. Thus, more attention should be paid to preserve RBCs when these components need transfer from plain to plateau regions. Recently, we found that mesenchymal stromal cells (MSCs) could rescue from anemia, and MSCs have been demonstrated in hematopoietic stem cells (HSCs) transplantation to reconstitute hematopoiesis in vivo by us. Considering the functions and advantages of MSCs mentioned above, we are trying to find out whether they are helpful to RBCs in storage duration at high altitudes. In the present study, we first found that mice MSCs could be preserved in citrate phosphate dextrose adenine-1 (CPDA-1) at 4 ± 2°C for 14 days, and still maintained great viability, even at plateau region. Thus, we attempted to use MSCs as an available supplement to decrease RBCs lesion during storage. We found that MSCs were helpful to support RBCs to maintain biochemical parameters and kept RBCs function well on relieving anemia in an acute hemolytic murine model. Therefore, our investigation developed a method to get a better storage of RBCs through adding MSCs, which may be applied in RBCs storage as a kind of cellular additive into preservation solution.

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Prolonged storage of red blood cells affects aminophospholipid translocase activity

Vox Sanguinis ◽

10.1111/j.1423-0410.2006.00822.x ◽

2006 ◽

Vol 91 (3) ◽

pp. 244-251 ◽

Cited By ~ 54

Author(s):

A. J. Verhoeven ◽

P. M. Hilarius ◽

D. W. C. Dekkers ◽

J. W. M. Lagerberg ◽

D. de Korte

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Prolonged Storage ◽

Aminophospholipid Translocase

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The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽

10.1182/blood.v54.1.280.280 ◽

1979 ◽

Vol 54 (1) ◽

pp. 280-284 ◽

Cited By ~ 27

Author(s):

E Beutler ◽

C West

Keyword(s):

Shelf Life ◽

Blood Cells ◽

Red Cells ◽

Normal Donor ◽

Red Cell ◽

Packed Red Blood Cells ◽

Citrate Phosphate Dextrose ◽

Storage Cells ◽

Stored Blood ◽

Citrate Phosphate

Abstract The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

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Does prolonged storage of red blood cells cause harm?

British Journal of Haematology ◽

10.1111/bjh.12747 ◽

2014 ◽

Vol 165 (1) ◽

pp. 3-16 ◽

Cited By ~ 64

Author(s):

Willy A. Flegel ◽

Charles Natanson ◽

Harvey G. Klein

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Prolonged Storage

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Prolonged storage of packed red blood cells for blood transfusion

Cochrane Database of Systematic Reviews ◽

10.1002/14651858.cd009330.pub2 ◽

2015 ◽

Cited By ~ 6

Author(s):

Arturo J Martí-Carvajal ◽

Daniel Simancas-Racines ◽

Barbra S Peña-González

Keyword(s):

Blood Transfusion ◽

Red Blood Cells ◽

Blood Cells ◽

Prolonged Storage ◽

Packed Red Blood Cells

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The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽

10.1182/blood.v54.1.280.bloodjournal541280 ◽

1979 ◽

Vol 54 (1) ◽

pp. 280-284

Author(s):

E Beutler ◽

C West

Keyword(s):

Shelf Life ◽

Blood Cells ◽

Red Cells ◽

Normal Donor ◽

Red Cell ◽

Packed Red Blood Cells ◽

Citrate Phosphate Dextrose ◽

Storage Cells ◽

Stored Blood ◽

Citrate Phosphate

The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

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Effect of storage on biochemical parameters of packed red blood cells of goats in............ 257-261 citrate phosphate dextrose adenine/ saline adenine glucose mannitol

Journal of Veterinary and Animal Sciences ◽

10.51966/jvas.2021.52.3.257-261 ◽

2021 ◽

Vol 52 (3) ◽

Author(s):

R. Thirupathy Venkatachalapathy ◽

R. Thirupathy Venkatachalapathy

Keyword(s):

Blood Cells ◽

Biochemical Parameters ◽

Reduced Glutathione ◽

Packed Red Blood Cells ◽

Storage Media ◽

Citrate Phosphate Dextrose ◽

Biochemical Studies ◽

Citrate Phosphate ◽

Packed Rbcs ◽

Apparently Healthy

A study was conducted to assess the suitability of citrate phosphate dextrose adenine / saline adenine glucose mannitol as a storage media for packed RBCs of goats. Samples collected from ten apparently healthy goats were utilized for the study. Biochemical studies were carried out on day 0, 14, 28 and 42 days of storage using the parameters, viz. pH, glucose, potassium, malondialdehyde and reduced glutathione. The pH was stable throughout the study, whereas glucose showed significant reduction. Rest of the parameters increased significantly from 0th day to 42nd day. Based on the results, the storage media can be considered to be suitable for storing caprine packed RBCs.

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