scholarly journals Cloning and sequencing of the rDNA gene family of the water buffalo (Bubalus bubalis)

2012 ◽  
Vol 11 (3) ◽  
pp. 2878-2883 ◽  
Author(s):  
C.Y. Pang ◽  
T.X. Deng ◽  
D.S. Tang ◽  
C.Y. Yang ◽  
H. Jiang ◽  
...  
Keyword(s):  
Gene Family ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Cloning And Sequencing ◽  
Rdna Gene

scholarly journals Molecular cloning, sequence characterization, and tissue expression analysis of three water buffalo (<i>Bubalus bubalis</i>) genes – <i>ST6GAL1</i>, <i>ST8SIA4</i>, and <i>SLC35C1</i>

2016 ◽  
Vol 59 (3) ◽  
pp. 363-372 ◽  
Author(s):  
Shen Song ◽  
Yina Ou-Yang ◽  
Jinlong Huo ◽  
Yongyun Zhang ◽  
Changlin Yu ◽  
...  
Keyword(s):  
Water Buffalo ◽  
Tissue Expression ◽  
Bubalus Bubalis ◽  
Pcr Analysis ◽  
Cloning And Sequencing ◽  
Hydrophobic Membrane ◽  
Sequence Characterization ◽  
Tissue Expression Analysis ◽  
Solute Carrier ◽  
Bovine Species

Abstract. Recent studies have shown that ST6 beta-galactosamide alpha-2,6-sialyltransferase 1 (ST6GAL1), ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (ST8SIA4), and solute carrier family 35, member C1 (SLC35C1) play essential roles in the metabolism of milk glycoconjugates in mammals. However, studies on their coding genes in water buffalo have not been reported. In the present study, cloning and sequencing showed that the coding sequences (CDSs) of buffalo ST6GAL1, ST8SIA4, and SLC35C1 were 1218, 1080, and 1095 bp in length, which encoded a precursor protein composed of 405, 359, and 364 amino acids, respectively. The deduced sequences of these three proteins in turn showed 97.6–98.5, 98.6–99.7, and 97.8–99.2 % similarities with other bovine species. Both buffalo ST6GAL1 and ST8SIA4 were predicted to be a member of glycosyltransferase family 29 and were all hydrophilicity proteins functioning in the Golgi apparatus. Buffalo SLC35C1 was a hydrophobic membrane protein located in the Golgi membrane, containing a TPT domain that is found in a number of sugar phosphate transporters. In addition, semi-quantitative RT-PCR analysis in 13 lactating buffalo tissues revealed that the ST6GAL1 and ST8SIA4 were expressed in 9 tissues, while SLC35C1 was expressed in 11 tissues. The expression levels of these three genes in the mammary gland were significantly higher in lactating than in non-lactating stage. Collectively, our data indicate that ST6GAL1, ST8SIA4, and SLC35C1 are potentially involved in the process of buffalo lactation.

scholarly journals Traumatic Reticuloperitonitis in Water Buffalo (Bubalus bubalis): Clinical Findings and the Associated Inflammatory Response

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Maged El-Ashker ◽  
Mohamed Salama ◽  
Mohamed El-Boshy
Keyword(s):  
Inflammatory Response ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Clinical Findings ◽  
Necrosis Factor Alpha ◽  
Reactive Protein ◽  
Amyloid A ◽  
Diagnostic Usefulness ◽  
Factor Alpha ◽  
Traumatic Reticuloperitonitis

The present study was carried out to describe the clinical picture of traumatic reticuloperitonitis (TRP) in water buffalo (Bubalus bubalis) and to evaluate the inflammatory and immunologic responses for this clinical condition. Twenty-two buffalo with acute local TRP were monitored in our study. Additionally, 10 clinically healthy buffalo were randomly selected and served as controls. Acute local TRP was initially diagnosed by clinical examination and confirmed by ultrasonographic (USG) examination and/or necropsy findings. Blood samples were collected from all examined buffalo to measure the respective levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, IL-10 and interferon gamma (INF)-γ, serum amyloid A (SAA), C-reactive protein (CRP), haptoglobin (Hp), fibrinogen (Fb), and serum sialic acid (SSA). It was found that TNF-α, IL-1β, IL-6, IL-10, SAA, CRP, Hp, Fb, and SSA were significantly higher in buffalo with TRP than the controls. Our findings suggest that the examined immunologic variables were helpful in documenting the inflammatory response in buffalo with TRP. However, their diagnostic usefulness only becomes apparent when considered in tandem with the clinical findings for any given animal, its anamnesis, and a subsequent USG assessment. Due to the frequent complications of TRP, more accurate indicators of its occurrence and severity would be useful.

Comparison of feed intake, digestibility and fattening performance of Brahman grade cattle (Bos indicus) and crossbred water buffalo (Bubalus bubalis)

2004 ◽  
Vol 75 (6) ◽  
pp. 549-555 ◽  
Author(s):  
Rosalina M. LAPITAN ◽  
Arnel N. DEL BARRIO ◽  
Osamu KATSUBE ◽  
Tomomi TOKUDA ◽  
Edgar A. ORDEN ◽  
...  
Keyword(s):  
Feed Intake ◽  
Water Buffalo ◽  
Bos Indicus ◽  
Bubalus Bubalis ◽  
Fattening Performance

scholarly journals Melanoma maligno em búfalos (Bubalus bubalis) albinos

2015 ◽  
Vol 36 (5) ◽  
pp. 3239 ◽  
Author(s):  
Maria Cecília Florisbal Damé ◽  
Clairton Marcolongo-Pereira ◽  
Letícia Fiss ◽  
Maria de Lourdes Adrién ◽  
Ana Lucia Schild
Keyword(s):  
Skeletal Muscle ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Renal Capsule ◽  
Southern Brazil ◽  
Epithelioid Cells ◽  
Melanocytic Tumors ◽  
Immunohistochemistry Staining ◽  
S 100 ◽  
Collagenous Stroma

<p>Two albino water buffalo affected by multiple melanocytic tumors in Southern Brazil are described. Grossly, there were multiple dark tumors within the skin, skeletal muscle, lungs, and lymph nodes. The tumor was also present in the pericardial sac, renal capsule, mediastinum and pleura. Microscopically, the tumors consisted of polyhedral epithelioid cells arranged in solid nests or interwoven fascicles supported by a thin and sparse collagenous stroma. The cytoplasm was eosinophilic and sometimes contained varying amounts of melanin pigment. The mitotic rates were low. Immunohistochemistry staining with Tyrosinase, Melan-A, Vimentin, S-100 protein, and neurofilament were positive. It is possible that the polymorphisms related to pigmentation in albino buffalo contributed with a high risk of developing melanoma as suggested in humans.</p>

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