scholarly journals Expression of hypoxia-inducible factor-1α during ovarian follicular growth and development in Sprague-Dawley rats

2015 ◽  
Vol 14 (2) ◽  
pp. 5896-5909 ◽  
Author(s):  
Z.H. Zhang ◽  
L.Y. Chen ◽  
F. Wang ◽  
Y.Q. Wu ◽  
J.Q. Su ◽  
...  
Keyword(s):  
Growth And Development ◽  
Hypoxia Inducible Factor ◽  
Follicular Growth ◽  
Sprague Dawley ◽  
Hypoxia Inducible Factor 1Α ◽  
Sprague Dawley Rats ◽  
Ovarian Follicular Growth

scholarly journals Silencing of Hypoxia-Inducible Factor-1α Gene Attenuated Angiotensin II–Induced Renal Injury in Sprague-Dawley Rats

Hypertension ◽  
2011 ◽  
Vol 58 (4) ◽  
pp. 657-664 ◽  
Author(s):  
Qing Zhu ◽  
Zhengchao Wang ◽  
Min Xia ◽  
Pin-Lan Li ◽  
Benjamin W. Van Tassell ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Renal Injury ◽  
Hypoxia Inducible Factor ◽  
Sprague Dawley ◽  
Hypoxia Inducible Factor 1Α ◽  
Sprague Dawley Rats

Mechanism of ischemic tolerance induced by hyperbaric oxygen preconditioning involves upregulation of hypoxia-inducible factor-1α and erythropoietin in rats

2008 ◽  
Vol 104 (4) ◽  
pp. 1185-1191 ◽  
Author(s):  
Guo-Jun Gu ◽  
Yun-Ping Li ◽  
Zao-Yun Peng ◽  
Jia-Jun Xu ◽  
Zhi-Min Kang ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Hyperbaric Oxygen ◽  
Target Gene ◽  
Molecular Mechanisms ◽  
Focal Cerebral Ischemia ◽  
Hypoxia Inducible Factor ◽  
Sprague Dawley ◽  
Triphenyltetrazolium Chloride ◽  
Time Points ◽  
Hypoxia Inducible Factor 1Α

We studied the effect of hyperbaric oxygen (HBO) preconditioning on the molecular mechanisms of neuroprotection in a rat focal cerebral ischemic model. Seventy-two male Sprague-Dawley rats were pretreated with HBO (100% O2, 2 atmospheres absolute, 1 h once every other day for 5 sessions) or with room air. In experiment 1, HBO-preconditioned rats and matched room air controls were subjected to focal cerebral ischemia or sham surgery. Postinjury motor parameters and infarction volumes of HBO-preconditioned rats were compared with those of controls. In experiment 2, HBO-preconditioned rats and matched room air controls were killed at different time points. Brain levels of hypoxia-inducible factor-1α (HIF-1α) and its downstream target gene erythropoietin (EPO) analyzed by Western blotting and RT-PCR as well as HIF-1α DNA-binding and transcriptional activities were determined in the ipsilateral hemisphere. HBO induced a marked increase in the protein expressions of HIF-1α and EPO and the activity of HIF-1α, as well as the expression of EPO mRNA. HBO preconditioning dramatically improved the neurobehavioral outcome at all time points (3.0 ± 2.1 vs. 5.6 ± 1.5 at 4 h, 5.0 ± 1.8 vs. 8.8 ± 1.4 at 8 h, 6.4 ± 1.8 vs. 9.7 ± 1.3 at 24 h; P < 0.01, respectively) and reduced infarction volumes (20.7 ± 4.5 vs. 12.5 ± 3.6%, 2,3,5-Triphenyltetrazolium chloride staining) after cerebral ischemia. This observation indicates that the neuroprotection induced by HBO preconditioning may be mediated by an upregulation of HIF-1α and its target gene EPO.

Ovarian Follicular Growth and Development During the Estrous Cycle of Bos taurus, Bos indicus and Bubalus bubalis Heifers Maintained on the Same Nutritional and Environmental Status.

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 675-675
Author(s):  
Julia M. Baldrighi ◽  
Manoel Francisco Sa Filho ◽  
Pietro Sampaio Baruselli ◽  
Jose Antonio Visintin ◽  
Mayra Elena Ortiz D'Avila Assumpcao
Keyword(s):  
Estrous Cycle ◽  
Growth And Development ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Bubalus Bubalis ◽  
Follicular Growth ◽  
Environmental Status ◽  
Ovarian Follicular Growth

scholarly journals Hypoxia Inducible Factor-2α Is Translationally Repressed in Response to Dietary Iron Deficiency in Sprague-Dawley Rats

2011 ◽  
Vol 141 (9) ◽  
pp. 1590-1596 ◽  
Author(s):  
McKale R. Davis ◽  
Krista M. Shawron ◽  
Elizabeth Rendina ◽  
Sandra K. Peterson ◽  
Edralin A. Lucas ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Hypoxia Inducible Factor ◽  
Dietary Iron ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals The mTORC1/eIF4E/HIF-1α Pathway Mediates Glycolysis to Support Brain Hypoxia Resistance in the Gansu Zokor, Eospalax cansus

2021 ◽  
Vol 12 ◽  
Author(s):  
Jinyan Lin ◽  
Lele Fan ◽  
Yuming Han ◽  
Juanjuan Guo ◽  
Zhiqiang Hao ◽  
...  
Keyword(s):  
Hypoxia Inducible Factor ◽  
Eukaryotic Cell ◽  
Metabolic Energy ◽  
Subterranean Rodents ◽  
Sprague Dawley ◽  
Subterranean Rodent ◽  
Downstream Targets ◽  
Metabolic Characteristics ◽  
Hypoxic Conditions ◽  
Sprague Dawley Rats

The Gansu zokor (Eospalax cansus) is a subterranean rodent species that is unique to China. These creatures inhabit underground burrows with a hypoxia environment. Metabolic energy patterns in subterranean rodents have become a recent focus of research; however, little is known about brain energy metabolism under conditions of hypoxia in this species. The mammalian (mechanistic) target of rapamycin complex 1 (mTORC1) coordinates eukaryotic cell growth and metabolism, and its downstream targets regulate hypoxia inducible factor-1α (HIF-1α) under conditions of hypoxia to induce glycolysis. In this study, we compared the metabolic characteristics of hypoxia-tolerant subterranean Gansu zokors under hypoxic conditions with those of hypoxia-intolerant Sprague-Dawley rats with a similar-sized surface area. We exposed Gansu zokors and rats to hypoxia I (44 h at 10.5% O2) or hypoxia II (6 h at 6.5% O2) and then measured the transcriptional levels of mTORC1 downstream targets, the transcriptional and translational levels of glycolysis-related genes, glucose and fructose levels in plasma and brain, and the activity of key glycolysis-associated enzymes. Under hypoxia, we found that hif-1α transcription was upregulated via the mTORC1/eIF4E pathway to drive glycolysis. Furthermore, Gansu zokor brain exhibited enhanced fructose-driven glycolysis under hypoxia through increased expression of the GLUT5 fructose transporter and ketohexokinase (KHK), in addition to increased KHK enzymatic activity, and utilization of fructose; these changes did not occur in rat. However, glucose-driven glycolysis was enhanced in both Gansu zokor and rat under hypoxia II of 6.5% O2 for 6 h. Overall, our results indicate that on the basis of glucose as the main metabolic substrate, fructose is used to accelerate the supply of energy in Gansu zokor, which mirrors the metabolic responses to hypoxia in this species.

scholarly journals Shaofu Zhuyu Decoction Regresses Endometriotic Lesions in a Rat Model

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Guanghui Zhu ◽  
Chunhua Jiang ◽  
Xin Yan ◽  
Shu Zhao ◽  
Dingjie Xu ◽  
...  
Keyword(s):  
Rat Model ◽  
Hypoxia Inducible Factor ◽  
Nuclear Antigen ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Sprague Dawley ◽  
Estrous Cycles ◽  
Treatment And Prevention ◽  
Sprague Dawley Rats ◽  
Current Therapies ◽  
Ectopic Endometrium

The current therapies for endometriosis are restricted by various side effects and treatment outcome has been less than satisfactory. Shaofu Zhuyu Decoction (SZD), a classic traditional Chinese medicinal (TCM) prescription for dysmenorrhea, has been widely used in clinical practice by TCM doctors to relieve symptoms of endometriosis. The present study aimed to investigate the effects of SZD on a rat model of endometriosis. Forty-eight female Sprague-Dawley rats with regular estrous cycles went through autotransplantation operation to establish endometriosis model. Then 38 rats with successful ectopic implants were randomized into two groups: vehicle- and SZD-treated groups. The latter were administered SZD through oral gavage for 4 weeks. By the end of the treatment period, the volume of the endometriotic lesions was measured, the histopathological properties of the ectopic endometrium were evaluated, and levels of proliferating cell nuclear antigen (PCNA), CD34, and hypoxia inducible factor- (HIF-) 1α in the ectopic endometrium were detected with immunohistochemistry. Furthermore, apoptosis was assessed using the terminal deoxynucleotidyl transferase (TdT) deoxyuridine 5′-triphosphate (dUTP) nick-end labeling (TUNEL) assay. In this study, SZD significantly reduced the size of ectopic lesions in rats with endometriosis, inhibited cell proliferation, increased cell apoptosis, and reduced microvessel density and HIF-1α expression. It suggested that SZD could be an effective therapy for the treatment and prevention of endometriosis recurrence.

FINASTERIDE INDUCES HYPOXIA-INDUCIBLE FACTOR 1-ALPHA IN THE PROSTATE OF SPRAGUE-DAWLEY RATS

2009 ◽  
Vol 181 (4S) ◽  
pp. 95-95
Author(s):  
Janmejai K Srivastava ◽  
Sanjeev Shukla ◽  
Cherry Kamel ◽  
Gregory T MacLennan ◽  
Allen D Seftel ◽  
...  
Keyword(s):  
Hypoxia Inducible Factor ◽  
Sprague Dawley ◽  
Hypoxia Inducible Factor 1 ◽  
Sprague Dawley Rats
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