scholarly journals Comparison of Loop-Mediated Isothermal Amplification (LAMP) and Conventional Culture for the Detection of Legionella Species in Hot Spring Water Samples in Japan

2005 ◽  
Vol 10 (3) ◽  
pp. 117-120 ◽  
Author(s):  
KATSUNORI FURUHATA ◽  
TOSHIMITSU ANNAKA ◽  
MASANARI IKEDO ◽  
MASAHUMI FUKUYAMA ◽  
SHIN-ICHI YOSHIDA
Keyword(s):  
Water Samples ◽  
Hot Spring ◽  
Spring Water ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Conventional Culture ◽  
Legionella Species

scholarly journals Radon Activity Concentrations in Natural Hot Spring Water: Dose Assessment and Health Perspective

2021 ◽  
Vol 18 (3) ◽  
pp. 920
Author(s):  
Eka Djatnika Nugraha ◽  
Masahiro Hosoda ◽  
June Mellawati ◽  
Untara Untara ◽  
Ilsa Rosianna ◽  
...  
Keyword(s):  
Public Health ◽  
Effective Dose ◽  
Radiation Protection ◽  
Water Samples ◽  
Hot Springs ◽  
Hot Spring ◽  
Annual Effective Dose ◽  
Spring Water ◽  
Dose Assessment ◽  
West Java

The world community has long used natural hot springs for tourist and medicinal purposes. In Indonesia, the province of West Java, which is naturally surrounded by volcanoes, is the main destination for hot spring tourism. This paper is the first report on radon measurements in tourism natural hot spring water in Indonesia as part of radiation protection for public health. The purpose of this paper is to study the contribution of radon doses from natural hot spring water and thereby facilitate radiation protection for public health. A total of 18 water samples were measured with an electrostatic collection type radon monitor (RAD7, Durridge Co., USA). The concentration of radon in natural hot spring water samples in the West Java region, Indonesia ranges from 0.26 to 31 Bq L−1. An estimate of the annual effective dose in the natural hot spring water area ranges from 0.51 to 0.71 mSv with a mean of 0.60 mSv for workers. Meanwhile, the annual effective dose for the public ranges from 0.10 to 0.14 mSv with an average of 0.12 mSv. This value is within the range of the average committed effective dose from inhalation and terrestrial radiation for the general public, 1.7 mSv annually.

scholarly journals Hydrochemical characteristics of the hot spring waters in the Kangding district related to the Lushan <i>M</i><sub>S</sub> 7.0 earthquake in Sichuan, China

2014 ◽  
Vol 2 (12) ◽  
pp. 7293-7308
Author(s):  
Z. Chen ◽  
X. Zhou ◽  
J. Du ◽  
C. Xie ◽  
L. Liu ◽  
...  
Keyword(s):  
Water Samples ◽  
Carbonate Rocks ◽  
Hot Springs ◽  
Hot Spring ◽  
Spring Water ◽  
Lushan Earthquake ◽  
Hydrochemical Characteristics ◽  
Hydrochemical Data ◽  
Deep Earth ◽  
Precipitation Water

Abstract. Hydrogeochemistry of 10 hot springs in the Kangding district was investigated by analyzing cation and anion concentrations of the spring waters. The water samples were collected within 5 days after the Lushan earthquake. The spring waters are classified into 7 chemical types based on the hydrochemical compositions. Comparison with the hydrochemical data before the Lushan earthquake, concentrations of Ca2+, HCO3− and TDS of the waters from the Guanding, Erdaoqiao, Gonghe, Erhaoying, Tianwanhe and Caoke springs evidently increased, which resulted from enhancing interaction between deep-earth fluids and carbonate rocks by the increment of dissolved CO2 in the groundwater. Concentrations of Na+, Cl− and SO42− of the waters from the Guanding, zheduotang, Xinxing and Gonghe springs were decreased, indicating dilution of precipitation water. Concentrations of Na+ and SO42− of the Erhaoying spring water increased, which may be attributed to the more supplement of fluids enriched in sulfur. The results indicate that hydrochemical components of spring water can be used as an effective indicator for earthquakes.

scholarly journals Direct detection of Mycobacterium avium in environmental water and scale samples by loop-mediated isothermal amplification

2013 ◽  
Vol 12 (2) ◽  
pp. 211-219 ◽  
Author(s):  
Yukiko Nishiuchi ◽  
Aki Tamaru ◽  
Yasuhiko Suzuki ◽  
Seigo Kitada ◽  
Ryoji Maekura ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Environmental Samples ◽  
Direct Detection ◽  
Culture Method ◽  
Environmental Water ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Culture Methods ◽  
Loop Mediated Isothermal Amplification ◽  
Conventional Culture

We previously demonstrated the colonization of Mycobacterium avium complex in bathrooms by the conventional culture method. In the present study, we aimed to directly detect M. avium organisms in the environment using loop-mediated isothermal amplification (LAMP), and to demonstrate the efficacy of LAMP by comparing the results with those obtained by culture. Our data showed that LAMP analysis has detection limits of 100 fg DNA/reaction for M. avium. Using an FTA® elute card, DNA templates were extracted from environmental samples from bathrooms in the residences of 29 patients with pulmonary M. avium disease. Of the 162 environmental samples examined, 143 (88%) showed identical results by both methods; 20 (12%) and 123 (76%) samples were positive and negative, respectively, for M. avium. Of the remaining 19 samples (12%), seven (5%) and 12 (7%) samples were positive by the LAMP and culture methods, respectively. All samples that contained over 20 colony forming units/primary isolation plate, as measured by the culture method, were also positive by the LAMP method. Our data demonstrate that the combination of the FTA elute card and LAMP can facilitate prompt detection of M. avium in the environment.

Assessment of the Campylobacter jejuni and C. coli in broiler chicken ceca by conventional culture and loop-mediated isothermal amplification method

Food Control ◽  
2017 ◽  
Vol 74 ◽  
pp. 107-111 ◽  
Author(s):  
Islam Ibrahim Sabike ◽  
Ryoko Uemura ◽  
Yumi Kirino ◽  
Hirohisa Mekata ◽  
Satoshi Sekiguchi ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Broiler Chicken ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Conventional Culture ◽  
Amplification Method

scholarly journals Growth in Acanthamoeba sp. and Antibiotic Susceptibility of Legionella micdadei Isolated from Hot Spring Water Samples

2009 ◽  
Vol 14 (4) ◽  
pp. 181-184 ◽  
Author(s):  
KATSUNORI FURUHATA ◽  
KIKUMI OGIHARA ◽  
RUMI OKUNO ◽  
KENJI OONAKA ◽  
MASAFUMI FUKUYAMA
Keyword(s):  
Water Samples ◽  
Antibiotic Susceptibility ◽  
Hot Spring ◽  
Spring Water ◽  
Legionella Micdadei

scholarly journals Hydrochemical Characteristics of Groundwater at the Epicenter of the 2021 Biru M6.1 Earthquake in Central Tibet

Water ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3111
Author(s):  
Pengtao Yang ◽  
Xiaolong Sun ◽  
Dongying Liu ◽  
Zhongtai He ◽  
Yongsheng Li
Keyword(s):  
Water Samples ◽  
Hot Spring ◽  
Groundwater Resources ◽  
Shallow Groundwater ◽  
Chemical Characteristic ◽  
Spring Water ◽  
Seismogenic Fault ◽  
Epicentral Area ◽  
Groundwater Environment ◽  
Central Tibet

Groundwater is undoubtedly important for water security and eco-environmental protection, especially in areas that experience earthquakes. Analyzing the characteristics and variation of groundwater after an earthquake is significant to obtain a better understanding of the seismic risk and rational management of groundwater resources. This study investigated the hydrogeochemical characteristics of groundwater at the epicenter of the 2021 Biru M6.1 earthquake in central Tibet, southwest China, using 23 water samples. The results showed that: (1) the hydrochemical type, hydrogen and oxygen isotope ratios, and SiO2 concentrations of three hot spring water samples in the study area were significantly different from those of samples taken elsewhere, indicating that the hot spring water originates from deeper geothermal reservoirs and has undergone more distant migration and longer fractionation processes; (2) the geochemical characteristics of groundwater from some sampling sites in the epicentral area were apparently distinct from those of other shallow groundwater or surface water samples, suggesting that the groundwater environment in the epicentral area has been affected by the earthquake. Along with the macroscopic groundwater responses in the epicentral area after the earthquake, we investigated the influencing mechanisms of the earthquake on the regional groundwater environment. We conclude that a shorter distance from the epicenter to the seismogenic fault leads to a greater possibility of the generation of new fractures, which then induce macroscopic responses and chemical characteristic variations for groundwater.

Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid and high-sensitive detection of Cryptosporidium in water samples

2009 ◽  
Vol 60 (8) ◽  
pp. 2167-2172 ◽  
Author(s):  
A. Inomata ◽  
N. Kishida ◽  
T. Momoda ◽  
M. Akiba ◽  
S. Izumiyama ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Water Samples ◽  
Cryptosporidium Oocyst ◽  
Lamp Assay ◽  
Test Tube ◽  
Isothermal Amplification ◽  
Sensitive Detection ◽  
Loop Mediated Isothermal Amplification ◽  
Cryptosporidium Oocysts ◽  
Amplification Assay

We describe a novel assay for simple, rapid and high-sensitive detection of Cryptosporidium oocysts in water samples using a reverse transcription-loop-mediated isothermal amplification (RT-LAMP). The assay is based on the detection of 18S rRNA specific for Cryptosporidium oocysts. The detection limit of the developed RT-LAMP assay was as low as 6 × 10−3 oocysts/test tube, which theoretically enables us to detect a Cryptosporidium oocyst and perform duplicated tests even if water samples contain only one oocyst. The developed RT-LAMP assay could more sensitively detect Cryptosporidium oocysts in real water samples than the conventional assay based on microscopic observation.

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