Optimization of extraction process of Chrysanthemi Flos by multi-index orthogonal experiment

2021 ◽  
Vol 251 ◽  
pp. 02058
Author(s):  
Feng Xuehua ◽  
Tao Ali ◽  
Song Zurong ◽  
Gong Panpan

The aqueous enzymatic method was applied to extract the common pistache oil and the optimal extraction process conditions were identified. By observing the effect of enzymatic hydrolysis time, pH value, temperature on aqueous enzymatic extraction process and performing the orthogonal experiment based on the single factor test, the optimal process parameters were obtained, namely, the optimal time, temperature, and pH value were respectively 3 h, 50℃, and 7 with a final extraction rate of 25.38 %.


2013 ◽  
Vol 13 (6) ◽  
pp. 463-466
Author(s):  
Qing GU ◽  
Wei SHU ◽  
DiShun YANG ◽  
XiaoJuan MA ◽  
JiYong LIU

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Zhang Jie ◽  
Li Chun-Yan ◽  
Li Jing-Ping ◽  
Guo Ren ◽  
Wang Hui ◽  
...  

The goal of this study is to investigate the immunoregulative effects ofPanax japonicuspolysaccharide (PJPS) on mice of low immunity. An orthogonal experiment was designed to determine the best extraction process for PJPS. By the tests of macrophages swallow chicken red blood cells, Delayed-type hypersensitivity (DTH), and serum hemolysin value, we studied the immune adjustment ability of PJPS. MTT was employed to detect the effects of different concentrations of PJPS, respectively, in 24 h, 48 h, and 72 h on five kinds of human cancer cells. The results show that the best extraction process for PJPS was as follows: ratio of solvent consumption to raw material 40, extraction temperature 100°C, re-extracted two times, each extraction time 4 hours. PJPS can significantly improve the immune function of mice processed by cyclophosphamide and PJPS did not work on the above five cancer cells.


2014 ◽  
Vol 1073-1076 ◽  
pp. 210-215 ◽  
Author(s):  
Hong Liu Zhang ◽  
Zhi Wei Wang ◽  
Peng Fei Xia

Department of Pterocarya stenoptera dicotyledonous juglandales juglandaceae Pterocarya plants, the whole body can be used as a medicine. This article use the continuous reflux method to studying the extraction process of Pterocarya stenoptera leaves of tannins in the area of Jiangsu Rugao. Through single factor experiment and orthogonal experiment to obtain continuous reflux method was used to extract Pterocarya stenoptera leaves of tannins the optimum technological conditions. Through to the extract of ultraviolet and GC-MS analysis results show that, different extraction agents, tannin content of the extractive and chemical composition are different. Deionized water and ethanol aqueous extract of difference for Pterocarya stenoptera leaves decoction and alcohol mechanism of two kinds of different uses for the treatment of disease research to provide the reference.


2014 ◽  
Vol 608-609 ◽  
pp. 1120-1127
Author(s):  
Qing Feng Guo ◽  
Wei Wang

Objective: To select the best process extracting protodioscin from trigonella foenum-graecum L. Methods: Methods: Orthogonal experiment method is used, and the content of protodioscin is used as the indicator. And the influence of ethanol solution concentration, the ratio of material to solvent, extraction time and extraction times on extraction rate of protodioscin from trigonella foenum-graecum L. And the best extraction process is selected. Results: 75% of ethanol is used, the ratio of material to solvent is 1:60, and ultrasound extraction is made for three times, each time for 15min, which is the best extraction process. The experiment proves that the optimization conditions are reliable and are suitable for ultrasound extraction process of protodioscin in trigonella foenum-graecum L.


2019 ◽  
Vol 78 ◽  
pp. 02014 ◽  
Author(s):  
Xiaoli Zhou ◽  
Guangxu Zhu ◽  
Yansheng Yang ◽  
Bin Du ◽  
Dong Lin

In this experiment, at first the roxburgh rose juice was extracted and then roxburgh rose residue was taken as raw material. We have used the enzyme assisted method to study the extraction process of polysaccharides from roxburgh rose pomace. The effects of mesh number, the concentration of the enzyme, temperature and time, pH and solid-liquid ratio on the polysaccharides yield were explored by single factor experiments. And then orthogonal experiment was designed to study the optimal techniques on extracting of polysaccharides from pomace. The in vitro antioxidant activity of the obtained polysaccharides was studied. The results showed that the optimal condition of extracting polysaccharides was as follows: enzyme concentration 2.5%, enzymatic hydrolysis at 60° for 40 min, pH 4.0, mesh number were 100 and solidliquid ratio was 1:25 based on the ratio of cellulase and pectinase was 2: 1. The average polysaccharides yield of enzymatic extraction method reached (4.79±0.07) % under the optimal condition. The antioxidant activity assays in vitro revealed that polysaccharides from roxburgh rose pomace can be used as natural antioxidants in functional foods and pharmaceutical industries.


IEEE Access ◽  
2020 ◽  
Vol 8 ◽  
pp. 190039-190048
Author(s):  
Zhe Ji ◽  
Jianyu Liu ◽  
Haijun Tian ◽  
Wang Zhang

2019 ◽  
Vol 21 (4) ◽  
pp. 66-71
Author(s):  
Shao Wenyao ◽  
Gong Xiao ◽  
Cui Shuming ◽  
Lin Ying ◽  
Lu Yinghua ◽  
...  

Abstract Hyaluronic acid (HA) has important applications in fields of health care products, cosmetics and clinical medical. However, the unique physiological properties of HA make cost of its traditional separation and extraction process relatively high. Foam separation technique has simple, gentle and efficient advantages on the separation of substances with surface activity by using bubbles as the separation medium. In this paper, natural surfactant CocamideBetaine (CAPB) was used as a foaming agent to explore the technology of microscale HA in aqueous solution by foam separation. The optimum process conditions were determined based on the recovery rate and enrichment ratio of HA by single factor and orthogonal experiment: at room temperature, pH = 7, separating air velocity (v) = 350 mL/min, HA concentration (CHA) = 50 mg/L, adding liquid volume (V) = 200 mL, collecting time (tcol) = 10 min, CAPB concentration (CCAPB) = 0.035 g/L. Under these conditions, HA enrichment ratio (E) equals 6.821 and HA recovery rate (R) equals 66.425%.


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