scholarly journals Propagation of Dendrobium antennatum Lindl via Seed Culture In Vitro Using Simple Medium: Fertilizer and Complex Organic Based Medium

2019 ◽  
Vol 26 (3) ◽  
pp. 133
Author(s):  
Julius Dwi Nugroho ◽  
Agustina Yohana Setyarini Arobaya ◽  
Evelyn Anggelina Tanur
Keyword(s):  
Seed Germination ◽  
Culture Medium ◽  
Coconut Water ◽  
Plantlet Production ◽  
Germination Process ◽  
Simple Medium ◽  
Spring Onion ◽  
Complex Organic ◽  
Pale Green

A New Guinea Orchid, Dendrobium antennatum Lindl urgently needs to conserve. The availability of in vitro germination technique for this orchids is significantly important to present the useful tool for conservation effort. This study aimed to find a simple media that consists of Growmore 10-55-10, a foliar fertilizer and complex organic i.e. coconut water, banana pulp, onion spring extract, and carrot juice. Five in vitro medium for seed germination and four in vitro sub-culture medium for planlet growth were formulated and used in this study. Our findings revealed that seed germination in vitro of Dendrobium antennatum well occured on the medium of Growmore 10-55-10 supplemented with 10% coconut water (GCw) and of Growmore 10-55-10 supplemented with 50 g/l extract spring onion. The seed germination process from sowing the seed to plantlet production ready to transfer to sub-culture medium for further growth took time 140 days. The embryo became pale green at the day 11 on the germination media. It could be used as a tool for detection of viability of the seeds. Both seed germination medium could be used also as sub-culture medium for enhancing the growth of the plantlets but not for multiplication of shoots.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

scholarly journals ASYMBIOTIC GERMINATION OF CYPRIPEDIUM CALCEOLUS VAR. PARVIFLORUM (ORCHIDACEAE) IN VITRO.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 618d-618
Author(s):  
Chin-Chang Chu ◽  
Kenneth W. Mudge
Keyword(s):  
Seed Germination ◽  
Suspension Culture ◽  
Agar Medium ◽  
Germination Rate ◽  
Coconut Water ◽  
Mature Seed ◽  
Orchid Species ◽  
Cypripedium Calceolus ◽  
Chilling Treatment

Seeds from mature seed pods of Cypripedium calceolus var. parviflorum were germinated on 1/4 MSMO (Sigma) + 100ml/l coconut water + 1% sucrose +/- 8g/l agar (pH 6.0), and with or without prechilling at 5C for 8 weeks. Protocorm with apex (stage 3) was use as an index of germination. Seeds sown on agar medium withou chilling treatment resulted in a 40% germination rate in 120 days but the germination was very uneven. Seeds germinated on agar medium with prechilling developed more synchronously with 92% germination in 60 days (ie. about 120 days after sowing). Suspension culture of seeds without prechilling resulted in 85% germination after 90 days. The synchronization of seed germination in suspension culture was intermediate between that on agar with and without prechilling. Protocorms germinated in suspension culture appeared morphologically identical to those germinated on agar medium. All stage 3 protocorms developed further on the same agar medium in darkness. Both agar and suspension culture in media containing coconut water provided reliable seed germination methods for this orchid species.

scholarly journals Enhanced Seed Germination and Seedling Growth of Tillandsia Eizii In Vitro

HortScience ◽  
2003 ◽  
Vol 38 (1) ◽  
pp. 101-104 ◽  
Author(s):  
Kimberly A. Pickens ◽  
James M. Affolter ◽  
Hazel Y. Wetzstein ◽  
Jan H.D. Wolf
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
Seedling Growth ◽  
Culture Medium ◽  
Habitat Destruction ◽  
Light Conditions ◽  
Physiological Constraints ◽  
Large Numbers ◽  
In The Wild

Tillandsia eizii is an epiphytic bromeliad that due to over-collection, habitat destruction, and physiological constraints has declined to near threatened status. This species exhibits high mortality in the wild, and seed are characterized by low percentages of germination. As a means to conserve this species, in vitro culture protocols were developed to enhance seed germination and seedling growth. A sterilization protocol using 70% ethanol for 2 minutes followed by 2.6% NaOCl for 40 minutes disinfested seed and promoted seedling growth. Sucrose incorporated into the culture medium had no effect on germination or growth, while NAA inhibited growth, but not germination. Cultures maintained under a 16-hour photoperiod at 22 °C exhibited greater growth than those grown at 30 °C. Seed that germinated in the dark remained etiolated and failed to develop even after transfer to light conditions. Plants grown in vitro were successfully acclimatized and transferred to the greenhouse. Over 86% survival and rapid growth were obtained with either an all-pine-bark medium, or a mixture of 2 redwood bark: 2 fir bark: 2 potting mix: 1 perlite. This demonstrated that in vitro culture of seed may be used to rapidly produce large numbers of T. eizii, and thus can be used for the conservation and reintroduction of this species.

scholarly journals Fungicide Seed Treatment Effects on Seedling Damping-off of Pumpkin Caused by Phytophthora capsici

Plant Disease ◽  
2003 ◽  
Vol 87 (1) ◽  
pp. 63-68 ◽  
Author(s):  
M. Babadoost ◽  
S. Z. Islam
Keyword(s):  
Seed Germination ◽  
Untreated Control ◽  
Mycelial Growth ◽  
Seed Treatment ◽  
Culture Medium ◽  
Phytophthora Capsici ◽  
Field Trials ◽  
Seedling Vigor ◽  
Damping Off

Apron XL LS (mefenoxam) and Allegiance FL (metalaxyl) were highly inhibitory to growth of mycelium of Phytophthora capsici in vitro. Effective dose (ED50) of mefenoxam and metalaxyl for 50% inhibition of mycelial growth, for all five isolates of P. capsici tested, was 0.98 and 0.99 μg a.i./ml of culture medium, respectively. For mefenoxam at 200 μg a.i./ml, sporangium and zoospore germination were reduced by 92 and 96%, respectively, and 21 and 24%, respectively, for metalaxyl. In greenhouse studies, seed treatment with mefenoxam (0.42 ml of Apron XL LS/kg of seed) and metalaxyl (0.98 ml of Allegiance FL/kg of seed) significantly reduced pre- and post-emergence damping-off of seedlings caused by P. capsici in three pumpkin cultivars (Dickinson, Hybrid-401, and Hybrid-698) tested. Thirty-one days after seeding, at inoculum levels of 0, 90, 600, 1,400, and 4,000 CFU/g of soil, the average seedling stands for mefenoxam treatment were 98.4, 93.8, 88.3, 77.8, and 64.8%; for metalaxyl, 99.1, 85.3, 85.8, 73.5, and 59.3; and for the untreated control, 97.5, 55.2, 45.7, 37.0, and 22.9%, respectively. In field trials, the average seedling stands 35 days after seeding were 76.7, 74.7, and 44.9% for mefenoxam, metalaxyl, and untreated control, respectively. Seed treatment with mefenoxam or metalaxyl did not have any significant effect on either seed germination or seedling vigor.

Alteration of extracellular cation concentrations and ratios in culture medium does not affect first cleavage division of hamster zygotes in vitro nor overcome the 'two-cell block'

1989 ◽  
Vol 1 (3) ◽  
pp. 231 ◽  
Author(s):  
BD Bavister ◽  
M Golden
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Cell Block ◽  
Cleavage Division ◽  
Cell Stage ◽  
Wide Range ◽  
Standard Culture ◽  
Simple Medium

In vivo fertilized hamster one-cell eggs (embryos) were cultured in a simple medium that was modified to provide a wide range of concentrations and ratios of the four major cation components (sodium, potassium, calcium and magnesium) while maintaining total osmotic pressure at 290 +/- 5 mosm. Embryos were cultured in these media to find the optimum cation concentrations for supporting the first cleavage division in vitro and to determine if physiologically abnormal cation concentrations and/or ratios in standard culture media could account for the 'two-cell block' to development in vitro in this species. Despite using a broad range of ratios for sodium:potassium (from 45:1 to 5:1) and for calcium:magnesium (from 17:1 to 1:1), there were no significant differences in the proportions of fertilized eggs that underwent the first cleavage division (approx. 60-80% across all treatments), and none of the two-cell embryos underwent further cleavage during extended culture. These data demonstrate that the first cleavage division of hamster embryos in vitro is insensitive to extracellular concentrations and ratios of the major cations, and that the non-physiological concentrations and/or ratios of these cations in the culture medium are not the primary reason for the failure of hamster zygotes to develop past the two-cell stage in vitro.

scholarly journals IN VITRO SEED GERMINATION AND PLANT GROWTH OF “CABEÇA-DE-FRADE” (CACTACEAE)

2021 ◽  
Vol 34 (1) ◽  
pp. 1-8
Author(s):  
SHEILA VITÓRIA RESENDE ◽  
ALONE LIMA-BRITO ◽  
GABRIELA TORRES- SILVA ◽  
JOSÉ RANIERE FERREIRA DE SANTANA
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Culture Medium ◽  
Ornamental Plants ◽  
In Vitro Cultivation ◽  
Apical Segment ◽  
Aerial Portion ◽  
Low Concentrations ◽  
Chemical Sterilization

ABSTRACT The genus Melocactus (“cabeça-de-frade”) comprises 32 species in Brazil, of which M. glaucescens and M. paucispinus are threatened with extinction. The present work evaluated the effects of different concentrations of Murashige & Skoog (MS, MS/2 and MS/4) culture medium and sucrose (15 g L-1 and 30 g L-1) on in vitro seed germination and plant growth of M. glaucescens and the efficiency of sterilization with sodium hypochlorite (NaOCl) on in vitro seed germination and plant growth of M. glaucescens when using seeds and M. glaucescens and M. paucispinus when using apical segment of cladode. In M. glaucescens, the final germination at the different MS and sucrose concentrations varied between 53.5 and 68.1% and the best results for in vitro growth were observed with the lowest mineral salt (MS/2 and MS/4) and sucrose (15 g L-1) concentrations, with lengths of the aerial portion of 9.70 and 10.76 mm, respectively. There was no difference in seed germination and plant growth in chemical and autoclave medium. It is concluded that the use of chemical sterilization with NaOCl at low concentrations of salts (MS/2 and MS/4) and sucrose (15 g L-1) are quite advantageous for producing ornamental plants germinated in vitro and/or apical segment of cladode of M. glaucescens andM. paucispinus, representing a reduction of costs for in vitro cultivation of this species.

scholarly journals Powdered coconut water (ACP 406®) as an alternative base culture medium for in vitro culture of goat preantral follicles enclosed in ovarian tissue

2019 ◽  
Vol 16 (4) ◽  
pp. 838-845
Author(s):  
Olga Juliana Roldan Castañeda ◽  
Francisco Léo Nascimento de Aguiar ◽  
Naiza Arcângela Ribeiro de Sá ◽  
Maria Luana Galdencio dos Santos Morais ◽  
Francielli Weber Santos Cibin ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Ovarian Tissue ◽  
Coconut Water ◽  
Preantral Follicles

scholarly journals Effects of culture medium and temperature on fungal growth of Mycena and marasmioid isolates and in vitro symbiotic culture in mycoheterotrophic orchid, Gastrodia pubilabiata

2021 ◽  
Author(s):  
Akane Shimazaki ◽  
Kana Higaki ◽  
Kento Rammitsu ◽  
Yuki Ogura-Tsujita
Keyword(s):  
Seed Germination ◽  
Culture Medium ◽  
Organic Materials ◽  
Agar Medium ◽  
Leaf Disc ◽  
Japanese Cedar ◽  
Malt Extract ◽  
Fungal Isolates ◽  
Mycorrhizal Association

In vitro symbiotic culture of Gastrodia pubilabiata seeds were conducted with the fungal isolates from G. pubilabiata roots. We obtained five fungal isolates, which belonged to Mycena, Marasmiaceae and Omphalotaceae. Firstly, optimal temperature and culture medium for subculture of these fungal isolates were examined. All five isolates grew the fastest on malt extract agar medium. Mycelial growth rate was highest at 25 °C between 10 °C and 40 °C. Secondly, we evaluated suitable culture vessels and organic materials for symbiotic culture. Seeds germinated well in petri dishes with Quercus leaf disc on water agar medium, and the seed germination process was well observed without dense mycelium. The most developed seedlings were found in glass bottles filled with Japanese cedar leaves, but densely grew mycelium prevent accurate seedling counts. Leaves of Quercus, Japanese cedar and bamboo were used as organic materials for symbiotic culture. All three leaves induced seed germination with Mycena and Marasmiaceae fungi, but material types affected subsequent seedling growth. Our method will contribute to understanding the mycorrhizal association of Gastrodia species and also other mycoheterotrophic plants.

scholarly journals Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

2017 ◽  
Vol 66 (4) ◽  
pp. 544-548 ◽  
Author(s):  
Seir Antonio Salazar Mercado ◽  
Nelson Alfonso Vega Contreras
Keyword(s):  
Seed Germination ◽  
Threatened Species ◽  
Growth And Development ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Ms Medium ◽  
Organic Additives ◽  
Natural Habitats ◽  
Asymbiotic Seed Germination

Cattleya trianae (Linden & Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of seedling formation after 20 weeks was found in MS + JP medium with significant differences (P <0.05: Tukey HSD). In this research, it is reported that the addition of organic additives to the MS medium improves the efficacy of this, and therefore, allows a greater growth and development of C. trianae under in vitro conditions.

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