scholarly journals Incidence of fungi and aflatoxin contamination of maize in Tolon-Kumbungu district of Ghana

2020 ◽  
Vol 28 (2) ◽  
pp. 195-202
Author(s):  
E.K. Aklaku ◽  
E.N.K. Sowley ◽  
M. Ofosu
Keyword(s):  
Zea Mays ◽  
Agar Plate ◽  
Sampling Technique ◽  
Northern Region ◽  
Aflatoxin Contamination ◽  
Sub Saharan Africa ◽  
Rapid Screening ◽  
Food And Agriculture ◽  
Aflatoxigenic Fungi ◽  
Sub Saharan

Maize (Zea mays L.) is an important staple food crop and a source of income to farmers, as well as foreign exchange earner in most countries in sub-Saharan Africa. Its production is hampered by fungal diseases, which also cause contamination with mycotoxins, especially aflatoxin and its associated health hazards. This study sought to isolate and identify aflatoxigenic fungi, as well as detect the presence of Aflatoxin B1 (AfB1) in maize samples obtained from farmers in the Tolon-Kumbungu district in the northern region of Ghana. Twenty farming communities were randomly selected for the study in consultation with the district office of the Ministry of Food and Agriculture (MoFA). Samples were collected from 200 randomly selected maize farmers by the composite sampling technique, for isolation of aflatoxigenic fungi by the agar plate method and the detection of aflatoxin. Aflatoxin was detected in maize samples with the Black light, rapid screening and immunoassay methods. Aspergillus flavus had the highest percentage of occurrence (63.7%); followed by A. niger (16.5%), Rhizopus stolonifer (9.3%), Penicillium spp. (6.9%) and Fusarium oxysporum (3.7%). Farm samples had more aflatoxin than those from stores and markets. Samples of maize from farms in Gbirimani community had the highest aflatoxin contamination of +60 ppb. Concentrations of Afb1 at or above +20 ppb were recorded in all the communities, except in Tinguli. Apart from Voggu, all market samples were free from aflatoxin contamination. Key words: Aflatoxigenic fungi, postharvest, Zea mays

scholarly journals Breeding maize ( Zea mays ) for Striga resistance: Past, current and prospects in sub‐saharan africa

2021 ◽  
Author(s):  
Abdoul‐Madjidou Yacoubou ◽  
Nouhoun Zoumarou Wallis ◽  
Abebe Menkir ◽  
Valerien A. Zinsou ◽  
Alexis Onzo ◽  
...  
Keyword(s):  
Zea Mays ◽  
Sub Saharan Africa ◽  
Striga Resistance ◽  
Sub Saharan

scholarly journals A Case for Regular Aflatoxin Monitoring in Peanut Butter in Sub-Saharan Africa: Lessons from a 3-Year Survey in Zambia

2016 ◽  
Vol 79 (5) ◽  
pp. 795-800 ◽  
Author(s):  
SAMUEL M. C. NJOROGE ◽  
LIMBIKANI MATUMBA ◽  
KENNEDY KANENGA ◽  
MOSES SIAMBI ◽  
FARID WALIYAR ◽  
...  
Keyword(s):  
South Africa ◽  
Aflatoxin B1 ◽  
Peanut Butter ◽  
Aflatoxin Contamination ◽  
Comprehensive Analysis ◽  
Sub Saharan Africa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Contamination Level ◽  
Batch Number ◽  
Sub Saharan

ABSTRACT A 3-year comprehensive analysis of aflatoxin contamination in peanut butter was conducted in Zambia, sub-Saharan Africa. The study analyzed 954 containers of 24 local and imported peanut butter brands collected from shops in Chipata, Mambwe, Petauke, Katete, and Nyimba districts and also in Lusaka from 2012 to 2014. For analysis, a sample included six containers of a single brand, from the same processing batch number and the same shop. Each container was quantitatively analyzed for aflatoxin B1 (AFB1) in six replicates by using competitive enzyme-linked immunosorbent assay; thus, aflatoxin contamination level of a given sample was derived from an average of 36 test values. Results showed that 73% of the brands tested in 2012 were contaminated with AFB1 levels >20 μg/kg and ranged up to 130 μg/kg. In 2013, 80% of the brands were contaminated with AFB1 levels >20 μg/kg and ranged up to 10,740 μg/kg. Compared with brand data from 2012 and 2013, fewer brands in 2014, i.e., 53%, had aflatoxin B1 levels >20 μg/kg and ranged up to 1,000 μg/kg. Of the eight brands tested repeatedly across the 3-year period, none consistently averaged ≤20 μg/kg. Our survey clearly demonstrates the regular occurrence of high levels of AF B1 in peanut butter in Zambia. Considering that some of the brands tested originated from neighboring countries such as Malawi, Zimbabwe, and South Africa, the current findings provide a sub-Saharan regional perspective regarding the safety of peanut butter.

Extension education strategy for minimizing aflatoxin impact on sub-Saharan African agriculture and food systems

2015 ◽  
Vol 8 (2) ◽  
pp. 253-257 ◽  
Author(s):  
A. Ayeni
Keyword(s):  
Foreign Exchange ◽  
Food Systems ◽  
Local Community ◽  
Aflatoxin Contamination ◽  
International Standards ◽  
Extension Education ◽  
Sub Saharan Africa ◽  
Extension Services ◽  
Policy Makers ◽  
Sub Saharan

Sub-Saharan Africa (SSA) is the most vulnerable region of the world to all aflatoxin-related problems including food insecurity, ill health and reduced foreign exchange earnings. Aflatoxin-contaminated maize, groundnuts (peanuts), sorghum and other crops reduce human access to adequate calories from these staples; consumption of aflatoxin-contaminated foods results in severe health conditions, including liver cancer, that deny the region a significant amount of otherwise productive man-hours; while the reduction of grain quality below the international standards due to aflatoxin contamination drastically reduces income in foreign exchange earnings. Scientific knowledge of the causes of aflatoxins in agricultural systems and their mitigation abounds in research institutions in SSA and internationally, but most of this knowledge is unavailable to farmers, food consumers and policy makers in useful form due to poor extension education and ineffective extension services. A paradigm shift in the approach to extension in SSA is proposed, one driven by a sustainable mechanism that is sensitive to the needs of the people and proactive (rather than reactive) in providing solutions to aflatoxin-related problems the local community and policy makers have to deal with. This paper argues that such sustainable mechanism may only be found in a University-based and University-run ‘land grant’ type extension services adapted appropriately to SSA conditions.

scholarly journals Stigmatising Attitudes Towards Co-workers with HIV in the Workplace of a Metropolitan State, Southwestern Nigeria

2017 ◽  
Vol 11 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Idongesit Godwin Utuk ◽  
Kayode Omoniyi Osungbade ◽  
Taiwo Akinyode Obembe ◽  
David Ayobami Adewole ◽  
Victoria Oluwabunmi Oladoyin
Keyword(s):  
Sampling Technique ◽  
Sub Saharan Africa ◽  
Good Knowledge ◽  
People Living With Hiv ◽  
Cross Sectional Survey ◽  
Southwestern Nigeria ◽  
Cross Sectional ◽  
Sub Saharan ◽  
Living With Hiv ◽  
Hiv Aids

Background:Despite demonstrating global concerns about infection in the workplace, very little research has explored how co-workers react to those living with HIV in the workplace in sub-Saharan Africa. This study aimed to assess the level of stigmatising attitude towards co-workers living with HIV in the workplace.Methods:The study was a descriptive cross-sectional survey involving 403 respondents. They were recruited from selected companies through a multistage sampling technique. Survey was carried out using pre-tested semi-structured questionnaires. Data were analyzed using the Statistical Package for the Social Sciences to generate frequencies, cross tabulations of variables at 5% level of significance. Logistic regression model was used to determine the predictors at 95% confidence intervals.Results:Mean age of respondents was 32.9 ± 9.4 years with 86.1% being females. Overall, slightly below two-third (63.0%) had good knowledge on transmission of HIV/AIDS while 218 (54.1%) respondents had a high stigmatising attitude towards co-workers with HIV in the workplace. More female respondents (69.6%) demonstrated high stigmatising attitudes towards co-workers with HIV in the workplace (p = 0.012). Female workers were twice more likely to have high stigmatising attitudes towards co-worker with HIV [OR 2.1 (95% CI: 1.13 – 3.83)].Conclusion:Stigma towards people living with HIV/AIDs is still very persistent in different settings. Good knowledge amongst our participants about HIV/AIDs did not translate to low stigmatising attitudes among workers. Concerted efforts and trainings on the transmission of HIV/AIDs are essential to reduce stigma that is still very prevalent in workplace settings.

scholarly journals Evaluating the Potential of Underdeveloped Land for Rice Production in Sub-Saharan Africa — A Case Study of Floodplains in the Northern Region of Ghana, West Africa

2017 ◽  
Vol 51 (1) ◽  
pp. 77-84
Author(s):  
Keiichi HAYASHI ◽  
Yasuhiro TSUJIMOTO ◽  
Tamao HATTA ◽  
Yukiyo YAMAMOTO ◽  
Jun-Ichi SAKAGAMI ◽  
...  
Keyword(s):  
West Africa ◽  
Northern Region ◽  
Rice Production ◽  
Sub Saharan Africa ◽  
Sub Saharan

The burden of foster children in Nigeria : deconstructing their existential realities and the will to survive

2021 ◽  
Vol 11 (1) ◽  
pp. 229-247
Author(s):  
Ezebunwa E. Nwokocha ◽  
Turnwait O. Michael
Keyword(s):  
Coping Strategies ◽  
Foster Children ◽  
Rational Choice Theory ◽  
Choice Theory ◽  
Sampling Technique ◽  
Sub Saharan Africa ◽  
Negative Consequences ◽  
Young Family ◽  
Child Fosterage ◽  
Sub Saharan

Child fosterage is one of the major manifestations of lack of capacity to cater for young family members in several societies of sub-Saharan Africa, which is reputed for prolific and sustained childbearing in the context of poverty. In Nigeria, the practice remains rife, with attendant negative consequences for foster children and communities in some cases. This paper, therefore, examined the challenges and coping strategies adopted by foster children in Bayelsa State, which was identified as one of the areas with high rates of child fosterage in Nigeria. Ethnomethodology and the rational choice theory were adopted as the theoretical framework. A total of 408 copies of a questionnaire were administered on respondents through a multistage sampling technique. Six in-depth interviews and two focus group discussions were conducted with foster children. The challenges reported by these children included physical abuse and maltreatment, lack of love and care, and lack of freedom of speech and boldness. Among the coping strategies identified by the foster children were endurance and silence, obedience and humility, and the uptake of paid jobs. There is, therefore, an urgent need for sustained fertility decline in order to discourage unnecessary child fosterage in Bayelsa and other states in Nigeria.

scholarly journals Transcriptomic Analysis of a Susceptible African Maize Line to Fusarium verticillioides Infection

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1112
Author(s):  
Humaira Lambarey ◽  
Naadirah Moola ◽  
Amy Veenstra ◽  
Shane Murray ◽  
Mohamed Suhail Rafudeen
Keyword(s):  
Zea Mays ◽  
Fusarium Verticillioides ◽  
Morphological Characteristics ◽  
Sub Saharan Africa ◽  
Chemical Stimulus ◽  
Post Inoculation ◽  
Rna Seq ◽  
Ear Rot ◽  
Maize Line ◽  
Sub Saharan

Maize (Zea mays L.) is a staple crop providing food security to millions of people in sub Saharan Africa. Fusarium verticillioides, an important fungal pathogen, infects maize causing ‘Fusarium Ear Rot’ disease, which decreases maize kernel yield and the quality of the crop harvested. Currently, no African maize line is completely resistant to infection by F. verticillioides. This study investigated an African maize line, Zea mays CML144, infected with F. verticillioides. Analysis of morphological characteristics showed significant differences between mock-infected and infected plants. RNA-sequencing (RNA-seq) was conducted on plants 14 days post-inoculation to identify differentially expressed genes (DEGs) involved in F. verticillioides infection. Analysis of RNA-seq data revealed DEGs that were both significantly up- and down-regulated in the infected samples compared to the mock-infected control. The maize TPS1 and cytochrome P450 genes were up-regulated, suggesting that kauralexins were involved in the CML144 defense response. This was substantiated by kauralexin analyses, which showed that kauralexins, belonging to class A and B, accumulated in infected maize tissue. Gene ontology terms relating to response to stimulus, chemical stimulus and carbohydrate metabolic processes were enriched, and the genes belonging to these GO-terms were down-regulated. Quantitative real-time PCR was performed on selected DEGs and measurement of phytoalexin accumulation validated the RNA-seq data and GO-analysis results. A comparison of DEGs from this study to DEGs found in F. verticillioides (ITEM 1744) infected susceptible (CO354) and resistant (CO441) maize genotypes in a previous study, matched 18 DEGs with 17 up-regulated and one down-regulated, respectively. This is the first transcriptomic study on the African maize line, CML144, in response to F. verticillioides infection.

scholarly journals Drivers of Labour Productivity in Flower Farms in Naivasha, Kenya

2017 ◽  
Vol 6 (4) ◽  
pp. 117
Author(s):  
Jared Isaboke Mose
Keyword(s):  
Survey Design ◽  
Labor Union ◽  
Labour Productivity ◽  
Sampling Technique ◽  
Sub Saharan Africa ◽  
Export Market ◽  
Skills Acquisition ◽  
Sub Saharan ◽  
Ict Infrastructure ◽  
Major Factors

Although Kenya is the most successful producer and exporter of fresh produce and flowers in sub-Saharan Africa, other countries both in Africa and elsewhere, offer strong competition that could erode export market share in future. Increased labor productivity is crucial for Kenya’s competitiveness. This study aimed at examining the key drivers of labour productivity in flower farms in Naivasha, Kenya. Descriptive survey design was employed and stratified proportionate random sampling technique used to select 381 respondents from who data was collected using a questionnaire. A log-linearized Cobb-Douglas model was used examine determinants of labour productivity. The results showed that workers’ participation in Labor unions, Information & Communication Technology and workers’ skills acquired through training were the major factors that determined labour productivity by 35.4 percent, 19 percent and 14.7 percent respectively. While worker’s wage increase and tools used by a worker influenced labour productivity by 9 percent and 11.4 percent respectively. Worker’s level of education and worker’s experience also increased labour productivity by 5.1 percent and 4 percent respectively. The study recommends that; the Kenyan government should give special attention to education to produce skilled and innovative workers. Flower Farms should invest more in training of workers to acquire relevant skills, acquisition of appropriate tools; improve ICT infrastructure and support labor union in the flower farms.

scholarly journals Determinants of Households’ Market Participation around Community Milk Cooling Plants, Western Kenya

2018 ◽  
Vol 10 (3) ◽  
pp. 204
Author(s):  
Justus I. Emukule ◽  
Mary J. Kipsat ◽  
Caroline C. Wambui
Keyword(s):  
Dairy Cow ◽  
Sampling Technique ◽  
Structured Interview ◽  
Household Size ◽  
Sub Saharan Africa ◽  
Primary Data ◽  
Market Participation ◽  
Household Characteristics ◽  
Sub Saharan ◽  
Multistage Sampling

Market participation in sub-Saharan Africa has been assessed mainly based on already producing households by looking at whether they sold or not, and if they sold, what quantities. The objective of this study was to determine the socio economic factors that influenced households’ decisions on market participation in terms of dairy cow ownership and quantity of milk sold while taking into consideration the non-producers using Heckman two stage model. The model allowed for not only determination of the effects of household characteristics on volume of milk surplus sold by already producing households but also drew inferences on the effect of household characteristics on probabilities of dairy cow ownership whileadding new information to literature by generating the truncation effect. A multistage sampling technique was used to select 544 producer and non-producer households and primary data collected using a semi structured interview schedule through personal interviews. From the results, probit marginal effects for dairy cow ownership were associated positively and statistically significant with household size, the level of education and land size owned by the households. The Heckman selection estimates revealed that increased number of dairy cows per household positively influenced the volumes of milk sold, while household size influenced negatively the quantity of milk sold. In conclusion, milk sales conditional on dairy cow ownership suffered from negative selectivity bias whereby a household with sample average characteristics who selected into dairy cow ownership secured 40% lower quantity of milk sold than would a household drawn at random.

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