The effect of “Gadagi” tea on liver function and serum glucose concentration in albino rats

Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.

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Effect of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2020.21.116 ◽

2020 ◽

Vol 21 (1) ◽

pp. 36-45

Author(s):

Huda Elbaz

Keyword(s):

Liver Function ◽

Oxidative Damage ◽

Hemoglobin Concentration ◽

Treated Group ◽

Serum Glucose ◽

Infected Group ◽

Fixed Dose ◽

Albino Rats ◽

Immunological Parameters ◽

Antioxidant Parameters

Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.

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Impact of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2020.21.106 ◽

2020 ◽

Vol 21 (1) ◽

pp. 36-45

Author(s):

Huda Elbaz ◽

Mohamed Hamed ◽

Fatma Abdelhamid ◽

Osama Abdalla

Keyword(s):

Liver Function ◽

Oxidative Damage ◽

Hemoglobin Concentration ◽

Treated Group ◽

Serum Glucose ◽

Infected Group ◽

Fixed Dose ◽

Albino Rats ◽

Immunological Parameters ◽

Antioxidant Parameters

Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.

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Impact of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2020.21.109 ◽

2020 ◽

Vol 21 (1) ◽

pp. 36-45

Author(s):

Huda Elbaz ◽

Mohamed Hamed ◽

Fatma Abdelhamid ◽

Osama Abdalla

Keyword(s):

Liver Function ◽

Oxidative Damage ◽

Hemoglobin Concentration ◽

Treated Group ◽

Serum Glucose ◽

Infected Group ◽

Fixed Dose ◽

Albino Rats ◽

Immunological Parameters ◽

Antioxidant Parameters

Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.

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Impact of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2020.21.006 ◽

2020 ◽

Vol 21 (1) ◽

pp. 36-45

Author(s):

Huda Elbaz ◽

Mohamed Hamed ◽

Fatma Abdelhamid ◽

Osama Abdalla

Keyword(s):

Liver Function ◽

Oxidative Damage ◽

Hemoglobin Concentration ◽

Treated Group ◽

Serum Glucose ◽

Infected Group ◽

Fixed Dose ◽

Albino Rats ◽

Immunological Parameters ◽

Antioxidant Parameters

Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.

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Effect of calcitonin on serum glucose concentration in rats.

Chemical and Pharmaceutical Bulletin ◽

10.1248/cpb.25.2189 ◽

1977 ◽

Vol 25 (9) ◽

pp. 2189-2194

Author(s):

MASAYOSHI YAMAGUCHI ◽

TAKEO YAMAMOTO

Keyword(s):

Glucose Concentration ◽

Serum Glucose

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A Comparative Study on the Effects of Using Hydroalcoholic Extracts of Linum Usitatissimum and Rosa Damascena on Liver Function in Adult Male Rats

Quarterly of Horizon of Medical Sciences ◽

10.32598/hms.26.1.3116.1 ◽

2020 ◽

Vol 26 (1) ◽

pp. 54-67

Author(s):

Mohammad Mehdi Padam ◽

◽

Ameneh Khoshvaghti ◽

Keyword(s):

Liver Function ◽

Total Protein ◽

Linum Usitatissimum ◽

Colorimetric Method ◽

Serum Glucose ◽

Male Rats ◽

Control Group ◽

Rosa Damascena ◽

Glucose Levels ◽

Significant Difference

Aims: Damage to liver tissue and its dysfunction is very important and if left untreated, it can cause serious problems and even death. In this study, we aimed to investigate the effects of the hydroalcoholic extracts of Linum usitatissimum and Rosa damascena on liver enzymes, total protein, bilirubin, albumin, and serum glucose levels. Materials and Methods: This is a non-randomized clinical trial conducted on 42 male rats divided into 6 groups; control group (group 1) received only sufficient water and food, groups 1 and 2 received 300 and 500 mg/ kgB.W Linum usitatissimum extract, groups 3 and 4 received 500 and 1000 mg/ kgB.W Rosa damascena, and group 6 received 100 mg/ kgB.W Linum usitatissimum plus 250 mg/ kgB.W Rosa damascena extracts intraperitoneally for 28 days. After the last injection, the rats were weighed and their blood samples were collected. The study parameters were measured using a colorimetric method by a spectrophotometer, and then were analyzed using ANOVA and Tukey’s test in SPSS V. 25 at a significance level of P<0.05. Findings: There was no significant difference between alanine aminotransferase, alkaline phosphatase, total and direct bilirubin levels in the control group in comparison with other groups (P>0.05). In the groups received Rosa damascena extract, there was a significant difference between total protein and albumin levels compared to the control group (P<0.05). Moreover, there was a significant difference between serum glucose and aspartate aminotransferase in the control group compared to other groups (P<0.05). Conclusion: Linum usitatissimum and Rosa damascena have no negative effect on the liver function. The probability of diarrhea occurrence and the possible effects on the total protein and serum albumin after using Rosa damascena, and the effects of different doses of Linum usitatissimum on the glucose levels should be taken into account.

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Successful Continuation of Peritoneal Dialysis after "Sweet" Hydrothorax

BANTAO Journal ◽

10.1515/bj-2015-0009 ◽

2015 ◽

Vol 13 (1) ◽

pp. 42-45

Author(s):

Utku Erdem Soyaltin ◽

Ferhat Ekinci ◽

Denizhan Ayatan ◽

Cihangir Turemis ◽

Mustafa Yildirim ◽

...

Keyword(s):

Peritoneal Dialysis ◽

Glucose Concentration ◽

Serum Glucose ◽

X Ray ◽

Glucose Levels ◽

Low Protein ◽

Elevated Glucose ◽

Chest X Ray ◽

Stage Renal Disease ◽

End Stage

AbstractA 44-year-old woman with end-stage renal disease presented with dyspnea on exertion and a vague chest pain about two weeks after commencing continuous ambulatory peritoneal dialysis (CAPD) four months ago. A chest x-ray revealed massive unilateral right-sided pleural effusion. Laboratory analysis of the effusion revealed low protein and lactate dehydrogenase but elevated glucose levels were consistent with transudate and pleuroperitoneal leakage. Pleural glucose concentration was much higher than patients’ serum glucose concentration, which was suggestive of "sweet" hydrothorax because of this high glucose concentration. It is advisable to keep this condition in mind among the differenttial diagnoses of hydrothorax in patients on CAPD.

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Deuterium-depleted Water Stimulates GLUT4 Translocation in the Presence of Insulin, which Leads to Decreased Blood Glucose Concentration

10.21203/rs.3.rs-117904/v1 ◽

2020 ◽

Author(s):

Miklós Molnár ◽

Katalin Horváth ◽

Tamás Dankó ◽

Ildikó Somlyai ◽

Bea Zs Kovács ◽

...

Keyword(s):

Glucose Metabolism ◽

Glucose Concentration ◽

Metabolic Regulation ◽

Natural Waters ◽

Blood Glucose Concentration ◽

Serum Glucose ◽

Diabetic Rat ◽

Dependent Manner ◽

Glut 4 ◽

Deuterium Depleted Water

Abstract BackgroundDeuterium (D) is a stable isotope of hydrogen (H) with a mass number of 2. It is present in natural waters in the form of HDO, at a concentration of 16.8 mmol/L, equivalent to 150 ppm. In a phase II clinical study, deuterium depletion reduced the fasting glucose concentration and insulin resistance.MethodsIn the study, we tested the effect of subnormal D-concentration on glucose metabolism in streptozotocin (STZ)-induced diabetic rat model. Animals were randomly distributed into 9 groups to test the effect of D2O (in a range of 25-150 ppm) on glucose metabolism in diabetic animals with or without 2X1 unit/day insulin treatment. Serum glucose, -fructose amine-, -HbAIC, –insulin, and urine glucose were tested. After the 8-week treatment, membrane associated GLUT-4 from soleus muscle content was estimated by Western blot technique.ResultsOur results indicate, that deuterium depletion in the presence of insulin reduced the serum glucose, -fructose amine, and -HbAIC, level on dose dependent manner. The optimal concentration of deuterium was between 125-140 ppm. After 8-week period of deuterium depletion the highest membrane-associated GLUT-4 content was detected at 125 ppm.ConclusionsThese data suggest that deuterium depletion dose-dependently enhances insulin’s effect on GLUT-4 translocation and potentiates glucose uptake in diabetic rats, which explains the lower serum glucose, -fructose amine, and -HbAIC concentrations. Based on our experimental data, deuterium-depleted water could be used to treat patients with metabolic syndrome (MS) by increasing the insulin sensitivity. The experiment indicates that the naturally occurring deuterium has an impact on metabolic regulation.

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Glucose-induced changes in pancreatic islet blood flow mediated by central nervous system

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1986.251.6.e644 ◽

1986 ◽

Vol 251 (6) ◽

pp. E644-E647 ◽

Cited By ~ 14

Author(s):

L. Jansson ◽

C. Hellerstrom

Keyword(s):

Central Nervous System ◽

Blood Flow ◽

Nervous System ◽

Glucose Concentration ◽

Serum Insulin ◽

Serum Glucose ◽

Insulin Concentration ◽

Similar Amount ◽

Serum Insulin Concentration ◽

Islet Blood Flow

Earlier experiments with the microsphere technique suggested that a heightened serum glucose concentration consistently leads to an increase in islet blood flow (IBF). Several lines of evidence suggest that this glucose-sensitive control mechanism is located at an extrapancreatic site. The purpose of this study was to define the possible role of the central nervous system in such a mechanism. D-glucose, L-glucose, 3-O-methylglucose, or saline were therefore infused into the carotid artery, each at a dose of 1 mg X kg body wt-1 X min-1 for 3 min, and the pancreatic and islet blood flows were measured. None of these substances affected the systemic serum glucose level. The intracarotid infusion of D-glucose, however, caused a rapid increase in both the serum insulin concentration and IBF. The blood flow to the whole pancreas nevertheless remained unchanged, indicating a redistribution of flow within the gland. Carotid infusion of the other test substances or a similar amount of D-glucose given in a femoral vein did not affect these parameters. Both the increase in serum insulin concentration and the increase in IBF caused by D-glucose could be abolished by vagotomy or administration of atropine. When the systemic blood glucose concentration was increased by intraperitoneal glucose administration (2 g/kg body wt), vagotomy blocked the increase in islet blood flow but not the concomitant insulin release. These observations suggest that the glucose-induced increase in IBF is mediated by vagal cholinergic influences.

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