scholarly journals The distribution of extended-spectrum Beta-Lactamase genes in Fomites, healthcare workers, and patients from two hospitals in Lagos state, Nigeria

2021 ◽  
Vol 23 (2) ◽  
pp. 15-24
Author(s):  
T. O. Egwuatu ◽  
O. D. Ishola ◽  
O. E. Oladele
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Healthcare Workers ◽  
Resistance Rate ◽  
Control Measures ◽  
Antibiotics Resistance ◽  
M Gene ◽  
Extended Spectrum ◽  
Lagos State

Antibiotics resistance is a rapidly emerging issue through the misuse of antibiotics to treat human and animalrelated infections. The use of beta-lactams has increased considerably since its discovery so also resistant genes leading to Extended-Spectrum Beta-Lactamases (ESBL) mediated by the presence of blaCTX-M , blaTEM and blaSHV genes present in most Gram-negative bacteria. This study aimed to detect the widespread distribution of ESBL genes from fomites, healthcare workers, and patients suffering from urinary tract infection in two hospitals in  Lagos state, Nigeria. A total of 150 swab samples were collected from fomites, health care workers, and cathetersof patients suffering from urinary tract infection (UTI). Antibiotics susceptibility test was performed by Kirby- Bauer technique according to CLSI guidelines. Organisms that tested positive phenotypically for ESBL were subjected to PCR for molecular analysis. ESBL prevalence rate of 21.8% and a carbapenemase-resistance rate of 16.7% were recorded. The ESBL producing isolates showed the highest resistance to ceftriaxone (82.4%) and the least resistance to tigecycline (5.9%). The existence of blaCTX-M and blaTEM was detected in 76.5% and 17.6% of the isolates respectively, while bla encoding gene was not detected in this study. The distribution of blaSHV genes detected in this study is of great concern which necessitates strict control measures in the usage of antibiotics especially the third-generation cephalosporin. In summary, the presence and distribution of ESBL encoding genes within two hospitals in Lagos were tested and the highest occurrence was recorded in blaCTX-M gene  reducing and limiting the available treatment option for infections.

Molecular Characterisation of ESBL producer E. coli

2018 ◽  
Vol 1 (2) ◽  
pp. 40-57
Author(s):  
Abdulghani Alsamarai ◽  
Shler Khorshed ◽  
Imad Weli
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Female Student ◽  
Clinical Problem ◽  
Clinical Isolates ◽  
Molecular Characterisation ◽  
M Gene ◽  
Esbl Producer ◽  
E Coli

Background: Antibiotic resistance emerged as clinical problem challenge the effective treatment of infections. Virulence factor may play an important role in the influence of antimicrobial resistance. Objective: To determine the frequency of resistance gene in E. coli clinical isolates from women with urinary tract infection. Materials and Methods: Fifteen E.coli clinical isolates were tested by PCR to determine their molecular characterization. Results: The bla CTX –M gene was not detected in 6.7% out of the tested 15 E. coli clinical isolates from women with urinary tract infection. However, bla OXA gene was detected in all E. coli tested clinical isolates from pregnant women, female student and diabetic women with urinary tract infection. While bla TEM gene and bla SHV gene were not detected in 33.3% and 40% out of the tested E. coli clinical isolates respectively. Conclusions: Four types of ESBL genes were detected, and shows new trend of distribution, which indicated the predominance of OXA and CTX-M genes.

Frequency of CTX-M gene isolated from E. coli in patients with suspected urinary tract infection in Semnan

Gene Reports ◽  
2021 ◽  
Vol 23 ◽  
pp. 101035
Author(s):  
Ali Akbar Shabani ◽  
Sima Ravaei ◽  
Hussein Anani ◽  
Reza Nasr ◽  
Mohammad Hussein Maroufi ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
M Gene ◽  
E Coli

scholarly journals Evaluation of ESBL (Extended Spectrum Beta-Lactamase) Producing Escherichia Coli Isolated from Patients with Urinary Tract Infection by Phenotypic and Genotypic Methods

2020 ◽  
Author(s):  
Mohammad Hasan Namaei ◽  
Hengameh Hamzei ◽  
Marzie Moghanni ◽  
Azadeh Ebrahimzadeh
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Use ◽  
M Gene ◽  
Esbl Producer ◽  
Accurate Identification ◽  
E Coli ◽  
Tract Infections

Abstract Background: Urinary Tract Infection (UTI) is the most common bacterial infection in the world. E. coli is the predominant Pathogen. This study evaluates the prevalence of ESBL in E. colis isolated from patients with urinary tract infections with phenotypic and genotypic methods.Methods: This descriptive-analytical study was done on 155 isolates of E. coli isolated from patients with urinary tract infection who had received the study consent. After accurate identification of E. coli strains. ESBL production for Escherichia coli isolates which are resistant to ceftriaxone or ceftazidime was evaluated by CDT method. TEM, SHV and CTX-M genes were identified by PCR.Results: The results showed that 30 strains from 155 strains of E. coli had ESBL. Strains of ESBL producer were more in males was lower in educated persons. 38.9% of ESBL producer had antibiotic use, 29.9% -producing Escherichia hospitalization and 31.6% uti history. The highest level of drug allergy in the ESBL was related to nitrofurantoin, and the highest resistance was related to cefazolin, co-trimoxazole. The CTX-M and the CTX-M15 gene were found in 92.7% and 57.1% of cases, respectively; also the SHV and TEM genes were not found in any of ESBL-producing Escherichia coli strains. Most therapeutic response in patients was related to cefexime, ciprofloxacin and nitrofurantoin 27.4%, 26% 21.9%, respectively.Conclusion: This study showed that the history of antibiotic use, hospitalization, uti related to increase of ESBL-producing in E. coli isolates., the CTMX-M gene is the most common gene in ESBL-producing E. coli strains.

scholarly journals Bacteriuria With CTX-M-8 Extended-Spectrum β-Lactamase–Producing Escherichia coli in a Patient With Incomplete Kawasaki Disease

2019 ◽  
Vol 6 ◽  
pp. 2333794X1882194
Author(s):  
Kaori Kamijo ◽  
Yoshifusa Abe ◽  
Takehi Kagami ◽  
Kazuhisa Ugajin ◽  
Takeshi Mikawa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Kawasaki Disease ◽  
Tract Infection ◽  
Antibiotic Treatment ◽  
Voiding Cystourethrography ◽  
Urine Specimen ◽  
Incomplete Kawasaki Disease ◽  
Extended Spectrum

We report the case of a 2-month-old infant with incomplete Kawasaki disease that presented as an apparent urinary tract infection. The patient’s fever persisted despite antibiotic treatment. Intravenous immunoglobulin and aspirin therapy cured both the incomplete Kawasaki disease and bacterial pyuria. Renal sonography, voiding cystourethrography, and renal parenchyma radionuclide scanning did not detect any abnormalities. Temporary dilation of the coronary artery was noted. In a urine specimen obtained through transurethral catheterization, the growth of 105 colony-forming units/mL of extended-spectrum β-lactamase–producing Escherichia coli was detected. Polymerase chain reaction analysis revealed that the enzyme genotype was CTX-M-8, which is a rare type in Japan. In conclusion, attention should be paid to a misleading initial presentation of fever and pyuria, which might be interpreted as urinary tract infection in patients with Kawasaki disease. Furthermore, pediatricians should consider incomplete Kawasaki disease when patients present with fever and pyuria, which are consistent with urinary tract infection, but do not respond to antibiotic treatment.

scholarly journals Resistance profile for pathogens causing urinary tract infection in a pediatric population, and antibiotic treatment response, at a university hospital 2010-2011

2014 ◽  
pp. 39-44 ◽  
Author(s):  
Catalina María Vélez Echeverri ◽  
Lina María Serna-Higuita ◽  
Ana Katherina Serrano Gayubo ◽  
Carolina Ochoa-García ◽  
Luisa Rojas Rosas ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pediatric Population ◽  
Resistance Rate ◽  
Empirical Treatment ◽  
Common Symptom ◽  
Female Ratio ◽  
E Coli ◽  
Study Results

Introduction: Urinary tract infection (UTI) is one of the most common bacterial infections in childhood and causes acute and chronic morbidity and long-term hypertension and chronic kidney disease. Objectives To describe the demographic characteristics, infectious agents, patterns of antibiotic resistance, etiologic agent and profile of susceptibility and response to empirical treatment of UTI in a pediatric population. Methods: This is a descriptive, retrospective study. Results: Included in the study were 144 patients, 1:2.06 male to female ratio. The most common symptom was fever (79.9%) and 31.3% had a history of previous UTI. 72.0% of the patients had positive urine leukocyte count (>5 per field), urine gram was positive in 85.0% of samples and gram negative bacilli accounted for 77.8% for the total pathogens isolated. The most frequent uropathogens isolated were Escherichia coli and Klebsiella pneumoniae. Our E.coli isolates had a susceptibility rate higher than 90% to most of the antibiotics used, but a resistance rate of 42.6% to TMP SMX and 45.5% to ampicillin sulbactam. 6.3% of E. coliwas extended-spectrum beta-lactamases producer strains. The most frequent empirical antibiotic used was amikacin, which was used in 66.0% of the patients. 17 of 90 patients who underwent voiding cistouretrography (VCUG) had vesicoureteral reflux. Conclusion: This study revealed that E. coli was the most frequent pathogen of community acquired UTI. We found that E. coli and other uropathogens had a high resistance rate against TMP SMX and ampicillin sulbactam. In order to ensure a successful empirical treatment, protocols should be based on local epidemiology and susceptibility rates.

Sign in / Sign up

Export Citation Format

Share Document