Evaluation of seed storage conditions on the growth of rough lemon (citrus jambhiri) rootstock seedlings

2005 ◽  
Vol 9 (1) ◽  
Author(s):  
RKA Egharevba ◽  
M Aiyeduyoni
Author(s):  
O. A. Zadorozhna ◽  
T. P. Shyianova ◽  
M.Yu. Skorokhodov

Seed longevity of 76 spring barley gene pool samples (Hordeum vulgare L. subsp. distichon, convar. distichon: 56 nutans Schubl., two deficience (Steud.) Koern., two erectum Rode ex Shuebl., two medicum Koern.; convar. nudum (L.) A.Trof.: one nudum L. та subsp. vulgare: convar. vulgare: nine pallidum Ser., three rikotense Regel.; convar. coeleste (L.) A.Trof.: one coeleste (L.) A.Trof.) from 26 countries, 11 years and four places of reproduction was analyzed. Seeds with 5–8% moisture content were stored in chamber with unregulated and 4oC temperature. The possibility of seed storage under these conditions for at least 10 years without significant changes in germination has been established. The importance of meteorological conditions in the formation and ripening of seeds for their longevity is confirmed. The relationship between the decrease of barley seeds longevity and storage conditions, amount of rainfall, temperature regime during the growing season of plants is discussed.


2012 ◽  
Vol 169 (14) ◽  
pp. 1401-1407 ◽  
Author(s):  
Hodaka Shishido ◽  
Yoko Miyamoto ◽  
Rika Ozawa ◽  
Shiduku Taniguchi ◽  
Junji Takabayashi ◽  
...  

1986 ◽  
Vol 93 (2) ◽  
pp. 231-239 ◽  
Author(s):  
S. Nemec ◽  
J. Syversten ◽  
Y. Levy

2000 ◽  
Vol 77 (11) ◽  
pp. 1648-1654
Author(s):  
Laura A Hyatt ◽  
Ann S Evans ◽  
Carol C Baskin

Patterns of germination over time were investigated in a short-lived desert perennial species, Lesquerella fendleri (Gray) S. Wats. Field-collected seeds were either buried in the field in cloth bags or stored in a glass jar under laboratory conditions. Regular germination tests were conducted under a range of alternating temperatures (buried seeds) or under differing water regimes (laboratory-stored seeds). Testing revealed Lesquerella fendleri to have an annual dormancy-nondormancy cycle, which was manifest regardless of seed storage conditions, suggesting that cycles may be partially endogenously regulated. Increasing seed germinability in field-stored seeds (maximum of 30% in year 1 and 95% in year 2) leads to the formation of a seed bank for at least 1 year and enhances the possibility of spreading germination events through time. This allows parent plants to maximize fitness in randomly fluctuating environments. The existence of two seedling flushes in the field and single germinability peaks in growth chambers suggests that dormancy cycling may support the formation of a metapopulation with subpopulations which are, although physically intermingled, genetically distinct.


2021 ◽  
Vol 42 ◽  
pp. e69341
Author(s):  
Miguel Ángel González Pérez ◽  
Nereida Cabrera-García ◽  
Isabel Cayon-Fernández

Conservation seed banks are essential for ex-situ conservation of genetic biodiversity. These institutions are especially relevant for threatened species and play a vital role in their conservation by preserving genetic material. However, samples deposited in the seed banks must germinate when necessary to use them (i.e., recovery plans, etc.). This study uses four accessions of the endemic endangered species from Gran Canaria Island (Canary Islands), Isoplexis isabelliana (Webb & Berthel.) Masf. (Scrophulariaceae). Germination tests were carried out to measure seed viability through time and the possible impact of seed storage on their viability. These accessions have been kept in the seed bank for four months to thirty years under different storage conditions. Germination results differed for seeds after 45 days of exposition using 16 hours light and 8 hours darkness at 17 °C. Accessions kept in the seed bank, independently of storage, showed a high germination percentage (89%). Whereas the accessions with rough storage conditions showed a 0% germination rate. The results highlighted the good state of conservation of the material deposited in the Seed Bank of the Botanical Garden "Viera y Clavijo" and the reliability of the temperature and humidity conditions in which the seeds of I. isabelliana have been stored. We consider these results as momentous since several natural populations of I. isabelliana has been affected by the last forest fire on the island.


Author(s):  
Samuel Adelani Babarinde ◽  
Kehinde Abike Kemabonta ◽  
Olagoke Zacchaeus Olatunde ◽  
Elizabeth Olajumoke Ojutiku ◽  
Adeyinka Kikelomo Adeniyi

Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Shidong Yue ◽  
Yu Zhang ◽  
Yi Zhou ◽  
Shaochun Xu ◽  
Shuai Xu ◽  
...  

Abstract Background Seagrass meadows are recognized as critical and among the most vulnerable habitats on the planet. The alarming rates of decline in seagrass meadows have attracted the attention globally. There is an urgent need to develop techniques to restore and preserve these vital coastal ecosystems. So far little work has been done to develop effective long-term storage method for seagrass seeds. The seagrass Zostera japonica Asch. & Graebn is an endangered species in its native range. Here we utilized combinations of different storage times, salinities, and temperature to determine the most appropriate conditions for optimal seed storage. Results Zostera japonica seeds were strongly desiccation sensitive, with a complete loss of viability after 24 h of desiccation. Therefore, long periods of exposure to air should be avoided to minimize seed mortality. In addition, Z. japonica seeds could not endure freezing conditions such as – 5 °C. However, our results indicated that reduced storage temperature to 0 °C could effectively prolong the duration of dormancy of Z. japonica seeds. Seeds stored at 0 °C under a salinity of 40–60 psu showed relatively low seed loss, high seed vigor and fast seed germination, suggesting these to be optimal seed storage conditions. For example, after storage for 540 days (ca. 600 days since the seed collection from reproductive shoots in early October, 2016) at 0 °C under a salinity of 50 psu, seeds still had a considerable vigor, i.e. 57.8 ± 16.8%. Conclusion Our experiments demonstrated that seeds stored at 0 °C under a salinity of 40–60 psu could effectively prolong the duration of dormancy of Z. japonica seeds. The proposed technique is a simple and effective long-term storage method for Z. japonica seeds, which can then be used to aid future conservation, restoration and management of these sensitive and ecologically important habitat formers. The findings may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.


2007 ◽  
Vol 55 (1) ◽  
pp. 18 ◽  
Author(s):  
Andrew D. Crawford ◽  
Kathryn J. Steadman ◽  
Julie A. Plummer ◽  
Anne Cochrane ◽  
Robin J. Probert

The suitability of applying international standards for long-term seed storage to the Australian flora was examined by reviewing seed-storage data from the Western Australian Threatened Flora Seed Centre. The 375 collections examined represented 176 taxa from 44 genera and 16 families. A high proportion of collections, representative of some of the most common genera in Australia, maintained viability in the short (<5 years) and medium (5–12 years) term. Declines in germination were evident for a small number of collections, representing 10 taxa, stored for 5–12 years. However, many of the declines were collection-specific and other collections of the same taxon did not decline. Five taxa showing germination declines were represented by a single collection so it was not possible to determine whether the germination decline was directly related to the taxa, storage conditions or to other factors. Only the closely related Stylidium coroniforme and S. amabile exhibited germination declines in all collections, but cut tests of seeds remaining from germination testing indicated that viability of the collections had not declined, just the proportion to germinate; for these species a change in germination conditions is a more likely explanation for the reduction in germination. The results illustrate the successful application of these seed-storage standards to threatened flora in Western Australia and highlight their suitability for the Australian flora.


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