Histological changes in kidney structure following a long – term administration of paracetamol (acetaminophen) in pregnant sprague dawley rats.

AbstractChemopreventive effect of non-steroidal antiinflammatory drugs (NSAIDs) in mammary carcinogenesis was reported in several studies. In this study, the effect of a nonselective cyclooxygenase inhibitor diclofenac (DICLO) in the prevention of N-methyl-N-nitrosourea (NMU)-induced mammary carcinogenesis in Sprague-Dawley female rats was evaluated. NMU was administered to animals intraperitoneally in two doses of 50 mg kg−1 b.w. within postnatal days 42-48. In experiment A (short-term administration), DICLO was administrated intramuscularly (5 mg kg−1 b.w.) every other day, starting 3 days before and for subsequent 25 days after first NMU injection. In experiment B (long-term administration), DICLO was administered in tap water (0.01 mg ml−1) continually, starting 7 days before and for subsequent 22 weeks after first NMU dose. The study was terminated 22 weeks after the first dose of NMU in both experiments. After DICLO treatment, tumor frequency per group was reduced in both variants of drug administration: in experiment A by 38% and in experiment B by 39.5%. Moreover, DICLO decreased tumor incidence by 11.5% and delayed tumor latency by 14 days in experiment B. In our preventive-curative experiments DICLO decreased some parameters of NMU-induced rat mammary carcinogenesis, mainly the tumor frequency.

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Role of antiglucocorticoid RU 486 on dexamethasone-induced hypertension in rats

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1989.256.5.e682 ◽

1989 ◽

Vol 256 (5) ◽

pp. E682-E685 ◽

Cited By ~ 1

Author(s):

M. Kalimi

Keyword(s):

Blood Pressure ◽

Term Treatment ◽

Sprague Dawley ◽

Ru 486 ◽

Sprague Dawley Rats ◽

Long Term Treatment ◽

Term Administration ◽

Slight Elevation

This study was conducted to investigate whether hypertension induced by long-term in vivo administration of dexamethasone in rats could be prevented by the newly synthesized potent antiglucocorticoid drug RU 486. Subcutaneous implantation of 5 mg of dexamethasone pellets in Sprague-Dawley rats resulted in a rapid increase in the blood pressure that remained elevated during the 3 wk of experimental observation. RU 486 (50 mg) administered alone surprisingly showed slight elevation of blood pressure over untreated control animals. However, the blood pressure leveled off to control levels over the next 2 wk. Interestingly, a 50-mg RU 486 pellet implanted along with 5 mg of dexamethasone effectively prevented the dexamethasone-induced increase in blood pressure. RU 486 administered together with dexamethasone prevented dexamethasone-induced diuresis and urinary Na+ excretion. However, RU 486 was unable to reverse the weight loss or involution of thymus observed by long-term treatment with dexamethasone alone. No abnormalities were found in either kidneys or hearts in any of the treated groups under microscopic examination. These results suggest that RU 486 successfully prevented the hypertension produced by the long-term administration of dexamethasone in male Sprague-Dawley rats.

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Induced gastrointestinal tract (GIT) derangement following a long-term administration of a non-selective cyclooxygenase inhibitor – paracetamol in pregnant Sprague–Dawley rats

Nigerian Journal of Health and Biomedical Sciences ◽

10.4314/njhbs.v5i1.11574 ◽

2006 ◽

Vol 5 (1) ◽

Author(s):

RE Ucheya ◽

JC Igweh ◽

MI Nweke

Keyword(s):

Gastrointestinal Tract ◽

Cyclooxygenase Inhibitor ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Term Administration

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Effect of long-term administration of Cinnamomum cassia silver nanoparticles on organs (kidneys and liver) of Sprague-Dawley rats

TURKISH JOURNAL OF BIOLOGY ◽

10.3906/biy-1805-103 ◽

2018 ◽

Vol 42 (6) ◽

pp. 498-505 ◽

Cited By ~ 1

Author(s):

Koffi KOUAME ◽

Aniekan Imo PETER ◽

Edidiong Nnamso AKANG ◽

Misturah ADANA ◽

Roshila MOODLEY ◽

...

Keyword(s):

Silver Nanoparticles ◽

Sprague Dawley ◽

Cinnamomum Cassia ◽

Sprague Dawley Rats ◽

Term Administration

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Ruxolitinib Alleviates Uveitis Caused by Salmonella typhimurium Endotoxin

Microorganisms ◽

10.3390/microorganisms9071481 ◽

2021 ◽

Vol 9 (7) ◽

pp. 1481

Author(s):

Lin Du ◽

Yolanda Wong Ying Yip ◽

Him Kwan Ng ◽

Bo Man Ho ◽

Jing-Na He ◽

...

Keyword(s):

Salmonella Typhimurium ◽

Inflammatory Arthritis ◽

Clinical Manifestations ◽

Janus Kinase ◽

Enteric Infection ◽

Sprague Dawley ◽

Proinflammatory Mediators ◽

Sprague Dawley Rats ◽

Term Administration

Uveitis is characterized by inflammatory lesions of intraocular structures. It is one of the important manifestations in patients with Reiter’s syndrome, an inflammatory arthritis, which is caused by enteric infection with bacteria, including Salmonella typhimurium. Corticosteroids remain the most frequently used therapies against uveitis associating with inflammatory arthritis. However, the long-term administration of steroids results in many side effects, and some uveitis patients do not respond to steroid treatment. Non-steroidal treatments are needed for uveitis patients. Our previous study found that Janus kinase (JAK) 1/2 inhibitor, ruxolitinib could suppress the expression of proinflammatory mediators in the ciliary body and iris. However, the impacts of ruxolitinib on ophthalmic features in uveitic eyes are still unknown. In this study, Salmonella typhimurium endotoxin-induced uveitis (EIU) was induced in Sprague Dawley rats by the injection of lipopolysaccharide (LPS). Compared with LPS-induced rats treated with water, ruxolitinib significantly attenuated the clinical manifestations, infiltrating cells and protein exudation in the aqueous humor, and retina–choroid thickening. Amplitudes of b-wave in both scotopic and photopic electroretinogram (ERG), and the amplitude of a-wave in scotopic ERG in EIU animals were alleviated by ruxolitinib. Collectively, we propose ruxolitinib could attenuate endotoxin-induced uveitis and rescue visual functions in rats by inhibiting the JAK2-STAT3 pathway.

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Effects of antimineralocorticoid RU 26752 on steroid-induced hypertension in rats

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1990.258.5.e737 ◽

1990 ◽

Vol 258 (5) ◽

pp. E737-E739

Author(s):

M. Kalimi ◽

J. Opoku ◽

M. Agarwal ◽

K. Corley

Keyword(s):

Experimental Period ◽

Placebo Control ◽

Sprague Dawley ◽

Control Value ◽

Sprague Dawley Rats ◽

Mineralocorticoid Antagonist ◽

Induced Hypertension ◽

Term Administration

The effect of mineralocorticoid antagonist RU 26752 on the development and maintenance of hypertension produced by long-term administration of mineralocorticoid agonist aldosterone has been investigated. Uninephrectomized, saline-drinking male Sprague-Dawley rats were subcutaneously implanted with either placebo (control) pellets or pellets containing 100 micrograms aldosterone, 50 mg RU 26752, or 100 micrograms aldosterone plus 50 mg RU 26752. Aldosterone treatment resulted in an increase in blood pressure to 165 +/- 5 mmHg over the control value of 105 +/- 2 mmHg within 3 wk of experimental period. RU 26752 given alone had no observable hypertensinogenic effect. However, RU 26752 administered with aldosterone significantly prevented the hypertension produced by aldosterone alone. RU 26752 when given with aldosterone was able to prevent the aldosterone-induced increase in saline consumption, increase urine output, and reduce urinary Na+ excretion. The results presented suggest that long-term administration of antimineralocorticoid RU 26752 in vivo to Sprague-Dawley rats prevents the aldosterone-induced hypertension.

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Effect of a Short-Term and Long-Term Melatonin Administration on Mammary Carcinogenesis in Female Sprague-Dawley Rats Influenced by Repeated Psychoemotional Stress

Acta Veterinaria Brno ◽

10.2754/avb200776030371 ◽

2007 ◽

Vol 76 (3) ◽

pp. 371-377 ◽

Cited By ~ 2

Author(s):

M. Kassayová ◽

E. Adámeková ◽

B. Bojková ◽

P. Kubatka ◽

I. Ahlers ◽

...

Keyword(s):

Mammary Carcinogenesis ◽

Tumour Volume ◽

Female Rats ◽

Sprague Dawley ◽

Short Term ◽

Psychoemotional Stress ◽

Sprague Dawley Rats ◽

Melatonin Administration ◽

Term Administration

The aim of this study was to evaluate the effect of melatonin (MEL) on N-methyl-N-nitrosourea (NMU)-induced mammary carcinogenesis in female Sprague-Dawley rats exposed to repeated psychoemotional stress - immobilization in boxes. NMU was applied intraperitoneally in two doses each of 50 mg/kg b.w. between 40 - 50 postnatal days. Melatonin was administered in drinking water at a concentration of 4 μg/ml daily from 15:00 h to 8:00 h. The application was initiated 5 days prior to the fi rst NMU dose and lasted 15 days, i.e. during the promotion phase of tumour development, or long-term until the end of the experiment (week 20). Immobilization (2 h per day) began on the third day after the second carcinogen application and lasted for 7 consecutive days. Short-term MEL administration to immobilized animals increased incidence by 22%, decreased tumour frequency per animal by 26% and reduced tumour volume gain (by 21%) when compared to the immobilized group without MEL application. Decreased frequency per animal by 28% and more than a 40% decrease in tumour volume gain and cumulative volume were the most pronounced changes in the animals drinking MEL until the end of the experiment. Long-term MEL administration reduced the number and size of mammary tumours more markedly than its short-term administration. Melatonin decreased certain attributes of mammary carcinogenesis in female rats influenced by psychoemotional stress.

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Toxicological evaluation of chronic oral administration of Melissa officinalis hydro-ethanol extract in Sprague-Dawley rats

Veterinary Science Development ◽

10.4081/vsd.2017.6298 ◽

2017 ◽

Vol 7 (1) ◽

Author(s):

Mohammad Hashemnia ◽

Farid Rezaei ◽

Zahra Nikousefat ◽

Maral Bahiraei

Keyword(s):

Mononuclear Cells ◽

Control Group ◽

High Dose ◽

Alcoholic Extract ◽

Sprague Dawley ◽

Melissa Officinalis ◽

Medicinal Uses ◽

Sprague Dawley Rats ◽

Term Administration

Melissa officinalis is a plant that has been widely used as an herbal medicine in many countries. Unfortunately, despite the prevalent medicinal uses of the plant, there are no reports on the possible toxic effects of M. officinalis. This study was designed to evaluate the effect of long-term administration of hydro-alcoholic extract of M. officinalis on some biochemical and hematological parameters and histopathology of organs. Thirty Sprague-Dawley rats were allocated to three equal groups. The animals in groups A and B received 600 and 1200 mg/kg M. officinalis extract, respectively, for 30 days. The rats in group C were given gavaged saline as control. The animals were euthanized at the end of experiment and the blood samples were collected for biochemical and hematology analysis. Additionally, appropriate tissue samples were collected from kidney, liver, spleen, heart and lung for light microscopic examination. M. officinalis caused a significant increase in the alanine aminotransferase level in the treated rats. Although the increase in creatine phosphokinase and lactate dehydrogenase levels were observed in group A and B, respectively, but there were no significant differences. A significant decrease was observed in the total protein and albumin concentrations in serum of treated rats as compared to the control group. The creatinine concentrations were significantly higher in the group B when compared to the other groups. There were no significant differences in cholesterol, triglyceride and urea concentrations between all groups of rats. The main histopathologic findings in the liver were included hepatocyte degeneration, congestion and dilation of sinusoids, proliferation of bile ducts and infiltration of mononuclear cells around the portal area. Histopathologic examination of the kidneys showed a tubular degeneration and necrosis, tubular and glomerular atrophy and congestion. These lesions were more prominent in the high dose treated rats. The findings suggest that long-term administration of M. officinalis extract even at low doses induces hepatic and renal lesions in rats.

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A diet with 35 % of energy from protein leads to kidney damage in female Sprague–Dawley rats

British Journal Of Nutrition ◽

10.1017/s0007114511000730 ◽

2011 ◽

Vol 106 (5) ◽

pp. 656-663 ◽

Cited By ~ 23

Author(s):

Andrew P. Wakefield ◽

James D. House ◽

Malcolm R. Ogborn ◽

Hope A. Weiler ◽

Harold M. Aukema

Keyword(s):

Creatinine Clearance ◽

Human Subjects ◽

Pig Model ◽

Sprague Dawley ◽

Histological Changes ◽

Monocyte Chemoattractant Protein 1 ◽

Protein Levels ◽

Sprague Dawley Rats ◽

Species Specific

High-protein (HP) diets for weight loss remain popular despite questions surrounding overall safety. In a recent study using the pig model, we showed that long-term intakes from whole proteins at 35 % energy (en %) cause moderate renal histological damage. To examine whether this observation may be species specific or more generalisable, the effect of this diet in rats was examined. Using plant and animal whole proteins, 70-d-old female Sprague–Dawley rats were randomised to either a normal-protein (NP; 15 en %) or a HP (35 en %) diet for 4, 8, 12 and 17 months. Renal function was assessed by creatinine clearance and urinary protein levels, and pathology was assessed by examination of glomerular hypertrophy, glomerulosclerosis and tubulointerstitial fibrosis. Rats consuming the HP diet had 17 % higher kidney weights (P < 0·0001), three times higher proteinuria (P < 0·0001) and 27 % higher creatinine clearance (P = 0·0012) compared with those consuming the NP diet. Consistent with this, HP-fed rats had larger glomeruli (P < 0·0001) and more glomerulosclerosis (P = 0·0003) compared with NP-fed rats. The HP diet also resulted in altered levels of free monocyte chemoattractant protein-1 (P < 0·0001). The histological changes are consistent with those observed in the pig model. In contrast to the pig model, the elevated proteinuria and creatinine clearance observed in the rat model are also usually observed with HP consumption in human subjects. These results indicate that the rat is a useful model for HP effects on the kidney and, along with previous results using the pig model, suggest that long-term intake of high levels of protein may be detrimental to renal health.

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