Seroprevalence and molecular detection of Newcastle disease virus in backyard chickens in Tripoli, Libya

Background: Newcastle disease (ND) is a viral disease that affecting many avian species all over the world.Aim: ND has been successfully controlled by the vaccination of commercial poultry in Libya. However, there was a lack of information about the situation of ND in backyard chickens. Therefore, this study determined the prevalence of ND in backyard chickens in different locations of Tripoli.Methods: A total number of 280 cloacal swabs (190 in summer and 90 in winter) and 412 sera were collected from non-vaccinated backyard chicken flocks in different geographical locations within the area of Tripoli namely Qasr Ben Ghashier, Al-Sawani, Souq Al-Gomaa, Tajourah, Ein Zara, and Janzour. Cloacal swabs and sera were tested by real time polymerase chain reaction (PCR) and ELISA, respectively.Results: The prevalence of ND virus (NDV) infection in backyard chickens in different locations of Tripoli during summer and winter was 45% using real-time reverse transcription-PCR. Except in Qasr Ben Ghashier, the prevalence in summer season was significantly higher than in winter (X2 = 46.13, p ≥ 0.00001). ELISA test revealed 218 positive out of 412 tested samples with total prevalence of 53% across the city of Tripoli in all regions. Obviously, Qasr Ben Ghashier had significantly (X2 = 74.09, p ≥ 0.00001) the highest prevalence (82%) of NDV specific antibodies followedby Tajourah (68%).Conclusion: This study demonstrated the situation of ND in backyard chicken highlighting the necessity of a comprehensive vaccination plan for backyard chickens. Keywords: Backyard chickens, ELISA, Newcastle disease, Prevalence, Real time PCR.

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Development of a real-time reverse-transcription PCR for the detection and simultaneous pathotyping of Newcastle disease virus isolates using a novel probe

Archives of Virology ◽

10.1007/s00705-009-0391-z ◽

2009 ◽

Vol 154 (6) ◽

pp. 929-937 ◽

Cited By ~ 27

Author(s):

Chad Marshal Fuller ◽

M. S. Collins ◽

D. J. Alexander

Keyword(s):

Newcastle Disease Virus ◽

Real Time ◽

Disease Virus ◽

Reverse Transcription ◽

Newcastle Disease ◽

Reverse Transcription Pcr ◽

Virus Isolates

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Respons Imun Ayam Petelur Pascavaksinasi Newcastle Disease dan Egg Drop Syndrome

Jurnal Sain Veteriner ◽

10.22146/jsv.29295 ◽

2017 ◽

Vol 35 (1) ◽

pp. 81

Author(s):

Gusti Ayu Kencana ◽

I Nyoman Suartha ◽

Daniel Raja Bonar Nainggolan ◽

Agatha Seren Lumban Tobing

Keyword(s):

Antibody Titre ◽

Newcastle Disease ◽

Egg Production ◽

Viral Disease ◽

Economic Losses ◽

Virus Envelope ◽

Post Vaccination ◽

Inactivated Vaccines ◽

Commercial Poultry ◽

Main Action

Some viral diseases in poultry could lead to huge losses to the farmers. Newcastle Disease (ND) and Egg Drop Syndrome (EDS) are a group of infectious viral disease that can cause the decreasein egg production. Newcastle Disease is caused by Avian paramyxovirus type 1 (PMV-1) Paramyxoviridae family. The causative agent of EDS is Duck adenovirus-I Adenoviridae family. Both of these diseases affect the economic losses to the poultry. The main action to prevent hens from ND and EDS virus diseases is vaccination. The success ofvaccination can be tested by serology. ND and EDS virus characteristically agglutinate hen’s erythrocyte they have Hemagglutine protein on virus envelope so can be tested by hemagglutination. The study was conducted ona commercial poultry farm in order to determine the success of vaccination against ND and EDS. The hens were vaccinated by Newcastle Disease-Infectious Bronchitis- Egg Drop Syndrome (ND-IB-EDS) inactivated vaccines.Serological test was conducted in pre and post vaccination by using microtiter hemagglutination test. The antibody titre is expressed in units of HI log2. The results of the study, the mean antibody titer against ND pre vaccinationwas 4,53 ± 1,356 HI log2 and antibody titre in 2nd, 3rd and 4th week were 8,67 ± 0,617 HI log2, 7,73 ± 1,335 HI log2 and 5,20 ± 0,862 HI log2 post vaccination. Antibody titre against EDS pre vaccination was 0 ± 0,000 HI log2 and antibody titre post vaccination in 2nd, 3rd and 4th week were 7 ± 1,363 HI log2, 7,27 ± 1,438 HI log2 and 7,6 ± 1,056 HI log2. It showed that ND-IB-EDS inactivated vaccines is serological protective for ND and EDS titres.

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Development of a Real-Time Reverse-Transcription PCR for Detection of Newcastle Disease Virus RNA in Clinical Samples

Journal of Clinical Microbiology ◽

10.1128/jcm.42.1.329-338.2004 ◽

2004 ◽

Vol 42 (1) ◽

pp. 329-338 ◽

Cited By ~ 251

Author(s):

M. G. Wise ◽

D. L. Suarez ◽

B. S. Seal ◽

J. C. Pedersen ◽

D. A. Senne ◽

...

Keyword(s):

Newcastle Disease Virus ◽

Real Time ◽

Disease Virus ◽

Reverse Transcription ◽

Newcastle Disease ◽

Clinical Samples ◽

Reverse Transcription Pcr ◽

Virus Rna

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Evaluation of Rapid Antigen Test for the Detection of Norovirus Infection: Comparison with ELISA and Real Time Quantitative Reverse Transcription PCR Assays

Laboratory Medicine Online ◽

10.3343/lmo.2011.1.4.3 ◽

2011 ◽

Vol 1 (4) ◽

pp. 184 ◽

Cited By ~ 4

Author(s):

Hyun Soo Kim ◽

Kyung Hee Kim ◽

Hye Won Kwon ◽

Tae Yeong Kang ◽

Mina Hur ◽

...

Keyword(s):

Real Time ◽

Reverse Transcription ◽

Antigen Test ◽

Quantitative Reverse Transcription Pcr ◽

Reverse Transcription Pcr ◽

Pcr Assays

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Thymic transcriptome analysis after Newcastle disease virus inoculation in chickens and the influence of host small RNAs on NDV replication

Scientific Reports ◽

10.1038/s41598-021-89464-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Liangxing Guo ◽

Zhaokun Mu ◽

Furong Nie ◽

Xuanniu Chang ◽

Haitao Duan ◽

...

Keyword(s):

Innate Immunity ◽

Newcastle Disease Virus ◽

Disease Virus ◽

Newcastle Disease ◽

Viral Disease ◽

Immune Genes ◽

Comprehensive Information ◽

Virulent Newcastle Disease Virus ◽

Chicken Thymus ◽

Innate Immune Genes

AbstractNewcastle disease (ND), caused by virulent Newcastle disease virus (NDV), is a contagious viral disease affecting various birds and poultry worldwide. In this project, differentially expressed (DE) circRNAs, miRNAs and mRNAs were identified by high-throughput RNA sequencing (RNA-Seq) in chicken thymus at 24, 48, 72 or 96 h post LaSota NDV vaccine injection versus pre-inoculation group. The vital terms or pathways enriched by vaccine-influenced genes were tested through KEGG and GO analysis. DE genes implicated in innate immunity were preliminarily screened out through GO, InnateDB and Reactome Pathway databases. The interaction networks of DE innate immune genes were established by STRING website. Considering the high expression of gga-miR-6631-5p across all the four time points, DE circRNAs or mRNAs with the possibility to bind to gga-miR-6631-5p were screened out. Among DE genes that had the probability to interact with gga-miR-6631-5p, 7 genes were found to be related to innate immunity. Furthermore, gga-miR-6631-5p promoted LaSota NDV replication by targeting insulin induced gene 1 (INSIG1) in DF-1 chicken fibroblast cells. Taken together, our data provided the comprehensive information about molecular responses to NDV LaSota vaccine in Chinese Partridge Shank Chickens and elucidated the vital roles of gga-miR-6631-5p/INSIG1 axis in LaSota NDV replication.

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