scholarly journals Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2

2020 ◽  
Vol 10 (2) ◽  
pp. 157-163
Author(s):  
María Carla García Mitacek ◽  
Romina Gisele Praderio ◽  
María Cecilia Stornelli ◽  
Rodolfo Luzbel De la Sota ◽  
María Alejandra Stornelli
Keyword(s):  
Stromal Cells ◽  
Endometrial Hyperplasia ◽  
Inflammatory Cells ◽  
Immunohistochemical Localization ◽  
Cyclooxygenase 2 ◽  
Immunohistochemical Expression ◽  
Normal Endometrium ◽  
Pathophysiological Mechanisms ◽  
Mixed Breed ◽  
Strong Staining

Background: In several mammals, subfertility or infertility associated with endometritis was reported. Although there have been studies about endometritis in bitches, the pathophysiological mechanisms are not completely known.Aim: This study aimed to evaluate the immunohistochemical expression of Cyclooxygenase 2 (COX2) in clinically healthy bitches with normal uterine tissue and bitches with endometritis.Methods: Forty-eight mixed breed bitches in diestrus were used. Uterine biopsies were collected for diagnosis [normal endometrium (n = 15; NE), cystic endometrial hyperplasia (n = 1), atrophy (n= 2), acute endometritis (n = 9; AE), subacute endometritis (n = 7; SE), and chronic endometritis (n = 14; CE)]. Immunostaining and quantification of positively stained cells was performed on full-thickness uterine biopsies. Data were analyzed by the GLIMMIX procedure of SAS.Results: COX2 immunostaining was scattered and restricted to cells in the stroma in bitches with NE. However, in bitches with endometritis, strong staining was observed in the luminal epithelium, glandular epithelium, and stromal cells. Staining was also observed in inflammatory cells localized in the stroma as well as inside of the glands. The percentage of COX2 positive stromal cells in bitches with AE, SE, and CE was significantly higher compared with NE (p < 0.005). In addition, the percentage of COX2 positive stromal cells in bitches with SE, and CE was significantly lower compared with AE (p < 0.003).Conclusion: COX2 could be involved in the pathophysiological mechanisms producing endometritis without the presence of cystic endometrial hyperplasia in bitches. However, further researches on this topic are required. Keywords: Bitch, COX2, Endometritis, Immunohistochemical.

Comparison of cyclooxygenase-2 and p53 expression in normal endometrium, endometrial hyperplasia and endometrial cancer

2006 ◽  
Vol 17 (3) ◽  
pp. 234
Author(s):  
Sang Wook Yoo ◽  
Ok Kyong Kim ◽  
Jae Yun Song ◽  
Soon Cheol Hong ◽  
Nak Woo Lee ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Endometrial Hyperplasia ◽  
Cyclooxygenase 2 ◽  
Normal Endometrium ◽  
P53 Expression

Estrogen and progesterone receptors and cyclooxygenase-2 expression in endometrial cancer, endometrial hyperplasia, and normal endometrium

2005 ◽  
Vol 97 (2) ◽  
pp. 483-488 ◽  
Author(s):  
Francisco J. Orejuela ◽  
Lois M. Ramondetta ◽  
Judith Smith ◽  
Jubilee Brown ◽  
Luciano B. Lemos ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Endometrial Hyperplasia ◽  
Progesterone Receptors ◽  
Cyclooxygenase 2 ◽  
Estrogen And Progesterone Receptors ◽  
Normal Endometrium

scholarly journals Epigenetic profile of endometrial proliferation in the different morphotypes of endometrial hyperplasia

2021 ◽  
pp. 68-78
Author(s):  
O.L. Gromova ◽  
V.O. Potapov ◽  
D.A. Khaskhachykh ◽  
O.P. Finkova ◽  
O.V. Gaponova ◽  
...  
Keyword(s):  
Cyclin D1 ◽  
Stromal Cells ◽  
Endometrial Hyperplasia ◽  
Nuclear Antigen ◽  
Secretory Phase ◽  
Normal Endometrium ◽  
Proliferative Phase ◽  
Epigenetic Profile ◽  
Glandular Cells ◽  
Er Expression

Research aim: to investigate the proliferative status of endometrium in the different morphotypes of endometrial hyperplasia based upon the identification of key molecular markers of the cell cycle.Materials and methods. Endometrial samples taken from 137 women were investigated: 40 – normal endometrium (NE), 61 – non-atypical endometrial hyperplasia (ЕH), 36 – atypical hyperplasia (AHE). Expression of gene cyclin D1, nuclear antigen Кі-67, glycoproteins Е-cadherin and β-catenin, estradiol receptors (ER) and progesterone receptors (PGR) were investigated. Results. ER expression of NE was high in the proliferative phase and decreased significantly in the secretory phase. PGR expression was high in both phases. ER expression of EH in glandular (180 ± 8.3) and in stromal cells (170.5 ± 4.1) exceed the indicators of the secretory phase. PGR expression in the stromal cells of EH (197.5 ± 9.3) exceed significantly indicators of NE. ER and PGR expression significantly and reliably decreased if there was AHE. ER expression of glandular cells was 2.6 times lower (74.6 ± 3.9) compere to proliferative NE (p <0.05) and 2.4 times lower to EH (р <0.05). ER of stromal AHE cells dropped to 30.3 ± 2.8, which was 5.5–5.6 times lower than in the proliferative NE and EH (p <0.002). PGR expression was 2.5–2.7 times lower (71.1 ± 2.3) in AHE glands than in NE and 2.8 times lower than in EH (p <0.05). Gene cyclin D1 expression was reliably increased in AHE cells compere to NE and EH. Protein Кі-67 expression in the glandular cells of EH was 2.6 times lower (p <0.05) and in AHE 2.9 times lower (p <0.05) than NE proliferative phase. We discovered strong direction to decreasing Е-cadherin expression in EH and it was lowest for AHE. Opposite direction was expression of β-catenin. The highest numbers of positive samples were observed in AHE and it was 100%. The highest numbers of negative β-catenin samples were in the NE cells (32,5–35%).Conclusion. The epigenetic profile investigation of endometrial hyperplasia will be useful for future development of carcinogenesis risk stratification, identifying patients with high risk of endometrial cancer and also for choosing the optimal way to influence the pathological process in the endometrium.

BAG-1 expression in normal endometrium, endometrial hyperplasia and endometrial cancer

2008 ◽  
Vol 87 (8) ◽  
pp. 862-867 ◽  
Author(s):  
Jae Yun Song ◽  
Ji Won Kim ◽  
Jae Kwan Lee ◽  
Nak Woo Lee ◽  
Bom Woo Yeom ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Endometrial Hyperplasia ◽  
Normal Endometrium

scholarly journals Loss of ten-eleven translocation 1 (TET1) expression as a diagnostic and prognostic biomarker of endometrial carcinoma

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259330
Author(s):  
Nien-Tzu Liu ◽  
Cherng-Lih Perng ◽  
Yu-Ching Chou ◽  
Pi-Shao Ko ◽  
Yi-Jia Lin ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Endometrial Carcinoma ◽  
Endometrial Hyperplasia ◽  
Expression Profiles ◽  
Gynecological Cancer ◽  
Dna Demethylation ◽  
Atypical Hyperplasia ◽  
Mrna Levels ◽  
Normal Endometrium ◽  
Expression Score

Endometrial carcinoma (EC) is the most common gynecological cancer. However, there is currently no routinely used biomarker for differential diagnosis of malignant and premalignant endometrial lesions. Ten-eleven translocation (TET) proteins, especially TET1, were found to play a significant role in DNA demethylation, via conversion of 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC). TET1, 5-mC, and 5-hmC expression profiles in endometrial carcinogenesis are currently unclear. We conducted a hospital-based retrospective review of the immunohistochemical expression of TET1, 5-mC, and 5-hmC in 181 endometrial samples. A “high” TET1 and 5-hmC expression score was observed in all cases of normal endometrium (100.0% and 100.0%, respectively) and in most samples of endometrial hyperplasia without atypia (90.9% and 78.8%, respectively) and atypical hyperplasia (90.6% and 93.8%, respectively), but a “high” score was found in only less than half of the EC samples (48.8% and 46.5%, respectively). The TET1 and 5-hmC expression scores were significantly higher in normal endometrium and premalignant endometrial lesions than in ECs (p < 0.001). A “high” 5-mC expression score was observed more frequently for ECs (81.4%) than for normal endometrium (40.0%), endometrial hyperplasia without atypia (51.5%), and atypical hyperplasia (53.1%) (p < 0.001). We also found that TET1 mRNA expression was lower in ECs compared to normal tissues (p = 0.0037). TET1 immunohistochemistry (IHC) scores were highly proportional to the TET1 mRNA levels and we summarize that the TET1 IHC scoring can be used for biomarker determinations. Most importantly, a higher TET1 score in EC cases was associated with a good overall survival (OS) rate, with a hazard ratio (HR) of 0.31 for death (95% confidence interval: 0.11–0.84). Our findings suggest that TET1, 5-mC, and 5-hmC expression is a potential histopathology biomarker for the differential diagnosis of malignant and premalignant endometrial lesions. TET1 is also a potential prognostic marker for EC.

scholarly journals Characterization of cell clusters in smears from normal endometrium, endometrial hyperplasia and well differentiated adenocarcinoma.

1995 ◽  
Vol 34 (3) ◽  
pp. 439-448 ◽  
Author(s):  
Yoshiaki NORIMATSU ◽  
Hiromi KODA ◽  
Shuji HAMAZAKI ◽  
Yuri OZEKI ◽  
Kyoumi NAKAKUNI ◽  
...  
Keyword(s):  
Endometrial Hyperplasia ◽  
Normal Endometrium ◽  
Cell Clusters ◽  
Well Differentiated
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