Effect of Alisma plantago-aquatica Linn extract on hyperprolactinemia in rats

Purpose: To investigate the anti-hyperprolactinemia effect and mechanism of action of of Alisma plantago-aquatica Linn. extract (APLE) in rats. Methods: The hyperprolactinemia (hyperPRL) model of rats was established by intraperitoneal (i.p.) metoclopramide (200 mg/kg daily) for 10 days. Sixty rats were divided into six groups (n = 10 each): normal group), hyperPRL control group, hyperPRL plus 0.6 mg/kg bromocriptine (as a positive control) group, and hyperPRL plus high (14.4 g/kg), medium (7.2 g/kg), or low (3.6 g/kg) dose of APLE. Bromocriptine or vehicle control was administered to the rats daily for 30 days, and the hypothalamus dopamine D2 receptor, protein kinase A (PKA), and cyclic adenosine monophosphate (cAMP) levels were investigated by Western blot. Results: Compared with the normal rats, hypothalamus dopamine D2 receptor protein expression was significantly lower in hyperPRL rats (p < 0.01), but was changed significantly after 30-day doses (various) of APLE administration (3.6 g/kg, p < 0.05; 7.2 and 14.4 g/kg, p < 0.01). Compared with the control rats, hypothalamus PKA and cAMP levels were significantly higher in hyperPRL rats (p < 0.01). These increases in PKA and cAMP were significantly attenuated by 30-day of bromocriptine treatment or various doses of APLE (p < 0.01). Conclusion: The anti-hyperPRL activity of APLE is confirmed from the findings of this study Thus, the plant can potentially be developed into a new anti-hyperprolactinemia drug.

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Effect of Zishenpingchan Granule on Neurobehavioral Manifestations and the Activity and Gene Expression of Striatal Dopamine D1 and D2 Receptors of Rats with Levodopa-Induced Dyskinesias

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/342506 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 5

Author(s):

Qing Ye ◽

Xiao-Lei Yuan ◽

Jie Zhou ◽

Can-xing Yuan ◽

Xu-ming Yang

Keyword(s):

Gene Expression ◽

Dopamine D2 Receptor ◽

D2 Receptor ◽

D2 Receptors ◽

Striatal Dopamine ◽

Control Group ◽

Indirect Pathway ◽

Dopamine D2 ◽

D1 And D2 Receptors ◽

Neurobehavioral Manifestations

This study was performed to observe the effects of Zishenpingchan granule on neurobehavioral manifestations and the activity and gene expression of striatal dopamine D1 and D2 receptors of rats with levodopa-induced dyskinesias (LID). We established normal control group, LID model group, and TCM intervention group. Each group received treatment for 4 weeks. Artificial neural network (ANN) was applied to excavate the main factor influencing variation in neurobehavioral manifestations of rats with LID. The results showed that overactivation in direct pathway mediated by dopamine D1 receptor and overinhibition in indirect pathway mediated by dopamine D2 receptor may be the main mechanism of LID. TCM increased the efficacy time of LD to ameliorate LID symptoms effectively mainly by upregulating dopamine D2 receptor gene expression.

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Water Extract ofFructus Hordei GerminatusShows Antihyperprolactinemia Activity via Dopamine D2 Receptor

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/579054 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Xiong Wang ◽

Li Ma ◽

En-jing Zhang ◽

Ji-li Zou ◽

Hao Guo ◽

...

Keyword(s):

Pc12 Cells ◽

Dopamine D2 Receptor ◽

D2 Receptor ◽

Water Extract ◽

Adenosine Monophosphate ◽

D2 Receptors ◽

Receptor Expression ◽

Gh3 Cells ◽

Serum Prolactin ◽

Dopamine D2

Objective.Fructus Hordei Germinatusis widely used in treating hyperprolactinemia (hyperPRL) as a kind of Chinese traditional herb in China. In this study, we investigated the anti-hyperPRL activity of water extract ofFructus Hordei Germinatus(WEFHG) and mechanism of action.Methods. Effect of WEFHG on serum prolactin (PRL), estradiol (E2), progesterone (P), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and hypothalamus protein kinase A (PKA) and cyclic adenosine monophosphate (cAMP) levels of hyperPRL rats were investigated. And effect of WEFHG on PRL secretion, D2 receptors, and dopamine transporters (DAT) was studied in MMQ, GH3, and PC12 cells, respectively.Results. WEFHG reduced the secretion of PRL in hyperPRL rats effectively. In MMQ cell, treatment with WEFHG at 1–5 mg/mL significantly suppressed PRL secretion and synthesis. Consistent with a D2-action, WEFHG did not affect PRL in rat pituitary lactotropic tumor-derived GH3 cells that lack the D2 receptor expression but significantly increased the expression of D2 receptors and DAT in PC12 cells. In addition, WEFHG reduced the cAMP and PKA levels of hypothalamus in hyperPRL rats significantly.Conclusions. WEFHG showed anti-hyperPRL activity via dopamine D2 receptor, which was related to the second messenger cAMP and PKA.

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Localization of dopamine D2 receptor protein in rat brain using polyclonal antibody

Brain Research ◽

10.1016/0006-8993(92)90253-6 ◽

1992 ◽

Vol 578 (1-2) ◽

pp. 244-250 ◽

Cited By ~ 24

Author(s):

Jeffrey W. Brock ◽

Shakeel Farooqui ◽

Keith Ross ◽

Chandan Prasad

Keyword(s):

Rat Brain ◽

Polyclonal Antibody ◽

Dopamine D2 Receptor ◽

D2 Receptor ◽

Receptor Protein ◽

Dopamine D2

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Preparation and characterization of an antibody specific to the rat dopamine D2 receptor

Journal of Endocrinology ◽

10.1677/joe.0.1340227 ◽

1992 ◽

Vol 134 (2) ◽

pp. 227-233 ◽

Cited By ~ 7

Author(s):

J. M. Johnston ◽

D. F. Wood ◽

J. D. James ◽

D. G. Johnston

Keyword(s):

Dopamine D2 Receptor ◽

D2 Receptor ◽

Cross Reactivity ◽

Receptor Protein ◽

Pituitary Cells ◽

Dopamine D2 ◽

Rat Pituitary ◽

Normal Rat ◽

Faint Band ◽

Rat Pituitary Cells

ABSTRACT Antibodies specific to the dopamine D2 receptor have been raised in rabbits using synthetic peptides. The resulting antiserum was sensitive to picogram quantities of peptide as measured by enzyme-linked immunoassay and was shown to have a 33% cross-reactivity with partially purified D2 receptor protein. No detectable cross-reactivity with similarly prepared fungal membranes was observed. D2 receptor preparations from normal rat pituitary cells were used in Western blot analysis. Bands of Mr = 95 000 and 34 000 were detected in these preparations with a third faint band at 120 000. These correspond to the pituitary D2 receptor. Journal of Endocrinology (1992) 134, 227–233

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The dopamine D2 receptor is expressed in GH3 cells

Journal of Molecular Endocrinology ◽

10.1677/jme.0.0070131 ◽

1991 ◽

Vol 7 (2) ◽

pp. 131-136 ◽

Cited By ~ 8

Author(s):

J. M. Johnston ◽

D. F. Wood ◽

E. A. Bolaji ◽

D. G. Johnston

Keyword(s):

Gene Expression ◽

Dopamine D2 Receptor ◽

Receptor Gene ◽

D2 Receptor ◽

Receptor Protein ◽

Gh3 Cells ◽

Pituitary Cells ◽

Dopamine D2 ◽

Receptor Gene Expression ◽

Gh3 Cell

ABSTRACT Some pituitary tumours respond to dopamine by decreasing the release of prolactin and/or GH and by inhibition of tumour growth. Certain tumours are unresponsive. Dopamine D2 receptor high-affinity binding is impaired in these tumours, and the rat GH3 cell line behaves in a similar way. The hypothesis that the dopamine-binding defect results from impaired D2 receptor gene expression has been tested in the present study. On Northern blots, D2 receptor mRNA was present in both normal rat pituitary cells and in GH3 cells. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis identified a putative D2 receptor protein in normal and GH3 cell membranes. The lack of effect of dopamine in GH3 cells does not reflect the absence of D2 receptor gene expression.

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