scholarly journals Analysis of the structural diversity of the microbial community in a paper-mill water system

Water SA ◽  
2002 ◽  
Vol 28 (4) ◽  
Author(s):  
Thea Van der Merwe ◽  
Karl-Heinz Riedel ◽  
Francois Wolfaardt
2015 ◽  
Vol 2015 ◽  
pp. 1-8
Author(s):  
Huirong Lin ◽  
Shuting Zhang ◽  
Song Gong ◽  
Shenghua Zhang ◽  
Xin Yu

The composition and microbial community structure of the drinking water system biofilms were investigated using microstructure analysis and 454 pyrosequencing technique in Xiamen city, southeast of China. SEM (scanning electron microscope) results showed different features of biofilm morphology in different fields of PVC pipe. Extracellular matrix material and sparse populations of bacteria (mainly rod-shaped and coccoid) were observed. CLSM (confocal laser scanning microscope) revealed different distributions of attached cells, extracellular proteins,α-polysaccharides, andβ-polysaccharides. The biofilms had complex bacterial compositions. Differences in bacteria diversity and composition from different tap materials and ages were observed. Proteobacteria was the common and predominant group in all biofilms samples. Some potential pathogens (Legionellales, Enterobacteriales, Chromatiales, and Pseudomonadales) and corrosive microorganisms were also found in the biofilms. This study provides the information of characterization and visualization of the drinking water biofilms matrix, as well as the microbial community structure and opportunistic pathogens occurrence.


1996 ◽  
Vol 34 (10) ◽  
pp. 105-112
Author(s):  
Svend-Erik Jepsen ◽  
Gert Holm Kristensen ◽  
Henrik Wenzel Christensen ◽  
Hans Henrik Knudsen ◽  
Anna Lise Mortensen ◽  
...  

Closing the water circuit in waste paper processing mills results in high concentrations of COD in the recirculating white water. Part of the COD is easily degradable and causes problems with microbiological growth in the water system. The load of dissolved COD to a paper mill producing moulded paper was determined: partly the incoming dissolved COD with the waste paper and partly the production of dissolved COD in the stock preparation and paper machine. Pilot-plant experiments with biofiltration document that it is possible to remove efficiently easily degradable COD from the recycling white water. Very high loads have been applied to the filter owing to the high temperature and easy degradable carbon. Controlling the dosage of nutrients is essential to avoid growth problems in the white water system. It is shown that nutrients can be dosed to the filter with concentrations close to zero in the effluent, and still achieve significant removal capacity of the filter.


2021 ◽  
Vol 44 ◽  
pp. 102407
Author(s):  
Hans Estrella Cainglet ◽  
Tomas Saavedra ◽  
Stefan Bürgmayr ◽  
Jianhua Zhang ◽  
Zongli Xie ◽  
...  

Author(s):  
Angelina S. Stenina ◽  
Elena N. Patova

The influence of the pulp-paper mill on the development of phytoplankton river communities in the wastewater discharge zones was studied. For the analysis, an indicator group of diatoms was used with data on species richness and structural diversity. The basis of the dominant complexes of the studied sections of the river were mainly species of the genus Aulacoseira. Saprobity indices vary by stations within 1.77–2.06, which corresponds to III class of water quality.


2006 ◽  
Vol 52 (5) ◽  
pp. 494-500 ◽  
Author(s):  
Tim J Dumonceaux ◽  
Janet E Hill ◽  
Carl P Pelletier ◽  
Michael G Paice ◽  
Andrew G Van Kessel ◽  
...  

We examined the microbial community structure and quantified the levels of the filamentous bulking organism Thiothrix eikelboomii in samples of activated sludge mixed liquor suspended solids (MLSS) from Canadian pulp and paper mills. Libraries of chaperonin 60 (cpn60) gene sequences were prepared from MLSS total microbial community DNA and each was compared with cpnDB, a reference database of cpn60 sequences (http://cpndb.cbr.nrc.ca) for assignment of taxonomic identities. Sequences similar to but distinct from the type strain of T. eikelboomii AP3 (ATCC 49788T) (~89% identity over 555 bp) were recovered at high frequency from a mill sample that was experiencing bulking problems at the time of sample collection, which corresponded to microscopic observations using fluorescent in situ hybridization with commercially available 16S rDNA-based probes. We enumerated this strain in five mill-derived MLSS samples using real-time quantitative PCR (qPCR) and found that two samples had high levels of the bulking strain (>1012genomes/g MLSS) and two contained lower but detectable levels of this organism. None of the mill samples contained cpn60 sequences that were identical to the type strain of T. eikelboomii. This technique shows promise for monitoring pulp and paper mill wastewater treatment systems by detecting and enumerating this strain of T. eikelboomii, which may be specific to pulp and paper mill wastewater treatment systems.Key words: activated sludge, biological treatment, bulking; chaperonin 60, cpn60, filamentous bacteria, mixed liquor suspended solids, microbial communities, 021N, qPCR, settling, Thiothrix.


2020 ◽  
Vol 35 (4) ◽  
pp. 649-659
Author(s):  
Xiaomin Lu ◽  
Anna Sundberg ◽  
Anders Strand ◽  
Martin A. Hubbe

AbstractThe influence of metal ions, {\mathrm{Ca}^{2+}} and {\mathrm{Mg}^{2+}}, on wood pitch retention at pH 8 was studied by investigating the pitch content of TMP-papers using both short- and long-column gas chromatography (GC). The effects of two different drying methods, air-drying and freeze-drying, on pitch retention were also compared in this work. The pitch emulsion was prepared with fatty acids, resin acids, and triglycerides at a certain ratio to simulate the pitch composition in closed water system in paper mill. At increasing pH, some of the resin and fatty acids will dissolve into the water phase as metal soaps. In this work, the retention of colloidal pitch in TMP-papers by metal ions at pH 8 was determined. The amount of 5 mM metal ions was found to retain more pitch. The tensile index decreased by the addition of metal ions, and the decrease became more pronounced as the increase of the metal ions concentration. Besides, the hydrophobicity of handsheets was found being changed only a little, which might because the morphology and pitch retained on the surface both affected the hydrophobicity.


Processes ◽  
2020 ◽  
Vol 8 (6) ◽  
pp. 723 ◽  
Author(s):  
Kishneth Palaniveloo ◽  
Muhammad Azri Amran ◽  
Nur Azeyanti Norhashim ◽  
Nuradilla Mohamad-Fauzi ◽  
Fang Peng-Hui ◽  
...  

Over the last decade, food waste has been one of the major issues globally as it brings a negative impact on the environment and health. Rotting discharges methane, causing greenhouse effect and adverse health effects due to pathogenic microorganisms or toxic leachates that reach agricultural land and water system. As a solution, composting is implemented to manage and reduce food waste in line with global sustainable development goals (SDGs). This review compiles input on the types of organic composting, its characteristics, physico-chemical properties involved, role of microbes and tools available in determining the microbial community structure. Composting types: vermi-composting, windrow composting, aerated static pile composting and in-vessel composting are discussed. The diversity of microorganisms in each of the three stages in composting is highlighted and the techniques used to determine the microbial community structure during composting such as biochemical identification, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), terminal restriction fragment length polymorphism (T-RFLP) and single strand-conformation polymorphism (SSCP), microarray analysis and next-generation sequencing (NGS) are discussed. Overall, a good compost, not only reduces waste issues, but also contributes substantially to the economic and social sectors of a nation.


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