Incidence and Recovery of Listeria from Chicken with a Pre-enrichment Technique

1990 ◽  
Vol 53 (7) ◽  
pp. 555-557 ◽  
Author(s):  
Y. VARABIOFF
Keyword(s):  
Listeria Monocytogenes ◽  
Water Samples ◽  
Listeria Innocua ◽  
Wash Water ◽  
Retail Stores ◽  
Enrichment Technique ◽  
Enrichment Procedure ◽  
Serotype 1

Eighty frozen chickens from four processors were purchased from retail stores in Brisbane. Forty-eight fresh chicken carcasses and 32 (16 hot and 16 chilled) wash-water samples from each of the four processors were also collected. The isolation of Listeria was achieved by a pre-enrichment procedure which allowed the recovery of injured cells. Listeria monocytogenes was isolated from 12 (15%) of the frozen chickens. Nine (11.2%) isolates were confirmed to be serotype 1 and three (3.8%) serotype 4. Fourteen (17.5%) of the frozen chickens were also contaminated by Listeria innocua. One (2.1%) sample of the fresh chickens yielded L. monocytogenes serotype 1 and five (10.4%) had L. innocua. L. monocytogenes serotype 1 was recovered from two (6.2%) samples of chilled wash water, but no Listeria were detected in hot wash water.

Incidence of Listeria monocytogenes in Raw Seafood Products in Japanese Retail Stores

2005 ◽  
Vol 68 (2) ◽  
pp. 411-415 ◽  
Author(s):  
SATOKO HANDA ◽  
BON KIMURA ◽  
HAJIME TAKAHASHI ◽  
TAKASHI KODA ◽  
KAZUO HISA ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Retail Stores ◽  
Serotype 1 ◽  
Seafood Products ◽  
Raw Fish ◽  
Fish Roe

The incidence of Listeria monocytogenes in raw fish, shellfish, and fish roe was investigated in seafood products collected from randomly selected retail stores in and around Tokyo, Japan. Of the 10 samples of 208 examined found positive for L. monocytogenes by mini-VIDAS LMO, seven were fish roe (cod, salmon) and three were minced tuna. Three serotypes (1/2a, 1/2b, 3b) were detected among the isolated strains; serotype 1/2a was predominant (8 of 10).

Prevalence of Listeria monocytogenes in Raw Milk in Guadalajara, Mexico

1995 ◽  
Vol 58 (10) ◽  
pp. 1139-1141 ◽  
Author(s):  
ANGELICA LUISJUAN-MORALES ◽  
RICARDO ALANIZ-DE LA O. ◽  
MARIA E. VÁZQUEZ-SANDOVAL ◽  
BEATRIZ T. ROSAS-BARBOSA
Keyword(s):  
Listeria Monocytogenes ◽  
Raw Milk ◽  
Listeria Innocua ◽  
Retail Stores ◽  
Street Vendors ◽  
Listeria Welshimeri ◽  
The City

The frequency of L. monocytogenes and other species of Listeria was determined in 100 samples of raw milk obtained from street vendors and retail stores located in the city of Guadalajara, Mexico. Listeria innocua and Listeria welshimeri were isolated from 7 and 2 samples, respectively, whereas L. monocytogenes was not isolated from any samples.

Recovery and Serotype Distribution of Listeria monocytogenes from Broiler Chickens in the Southeastern United States

1989 ◽  
Vol 52 (3) ◽  
pp. 148-150 ◽  
Author(s):  
J. S. BAILEY ◽  
D. L. FLETCHER ◽  
N. A. COX
Keyword(s):  
United States ◽  
Listeria Monocytogenes ◽  
Broiler Chickens ◽  
Southeastern United States ◽  
Retail Stores ◽  
Serotype Distribution ◽  
Epidemiological Evidence ◽  
Listeria Species ◽  
Processing Plants ◽  
Serotype 1

A total of ninety broiler carcasses from three processing plants were obtained from retail stores in the southeastern United States. The optimum plating medium was determined and carcasses were rinse sampled and the recovery of Listeria monocytogenes and other Listeria species determined. Presumptive L. monocytogenes isolates were serotyped. Listeria were recovered from 34 of 90 (38%) of the carcasses sampled, while L. monocytogenes were recovered from 21 of 90 (23%) of the carcasses sampled. Of the 35 L. monocytogenes isolates serotyped, 21 (64%) were confirmed to be serotype 1/2 b and 6 (18%) were confirmed to be serotype 1/2 c. Although there is no epidemiological evidence to suggest a relationship between consumption of chicken and listeriosis, the presence of L. monocytogenes on 23% of sampled broilers emphasizes the importance of maintaining hygienic practices in production, processing and preparation of fresh broilers.

scholarly journals Environmental dissemination of pathogenic Listeria monocytogenes in flowing surface waters in Switzerland

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Susanne Raschle ◽  
Roger Stephan ◽  
Marc J. A. Stevens ◽  
Nicole Cernela ◽  
Katrin Zurfluh ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Surface Water ◽  
Food Chain ◽  
Water Samples ◽  
Opportunistic Pathogen ◽  
Whole Genome Sequence ◽  
Serotype 1 ◽  
Sporadic Cases ◽  
Serotype 4B ◽  
Surface Water Samples

AbstractListeria monocytogenes is an opportunistic pathogen that is widely distributed in the environment. The aquatic environment may represent a potential source for the transmission of L. monocytogenes to animals and the food chain. The present study assessed the occurrence of L. monocytogenes in 191 surface water samples from rivers, streams and inland canals throughout Switzerland. Twenty-five (13%) of the surface water samples contained L. monocytogenes. Whole genome sequence (WGS) data were used to characterize the 25 isolates. The isolates belonged to major lineages I and II, with the majority assigned to either serotype 1/2a (48%), or 4b (44%). The predominant CCs identified were the hypervirulent serotype 4b clones CC1 and CC4, and the serotype CC412; all three have been implicated in listeriosis outbreaks and sporadic cases of human and animal infection worldwide. Two (8%) of the isolates belonged to CC6 which is an emerging hypervirulent clone. All isolates contained intact genes associated with invasion and infection, including inlA/B and prfA. The four CC4 isolates all harbored Listeria pathogenicity island 4 (LIPI-4), which confers hypervirulence. The occurrence of L. monocytogenes in river ecosystems may contribute to the dissemination and introduction of clinically highly relevant strains to the food chain.

Incidence of Listeria monocytogenes in Cheese Produced in Rio de Janeiro, Brazil

1998 ◽  
Vol 61 (3) ◽  
pp. 354-356 ◽  
Author(s):  
MARIA CRISTINA DELGADO da SILVA ◽  
ERNESTO HOFER ◽  
ANITA TIBANA
Keyword(s):  
Listeria Monocytogenes ◽  
Rio De Janeiro ◽  
Health Protection ◽  
Listeria Innocua ◽  
Retail Stores ◽  
White Cheese ◽  
The World ◽  
Sporadic Cases ◽  
Listeria Welshimeri

The present study evaluated the incidence of Listeria spp. in some Brazilian cheeses obtained from retail stores in Rio de Janeiro. Of 103 samples of various types of cheese examined as recommended in the Listeria isolation protocol of the Health Protection Branch of Canada, 11 (10.68%) were contaminated by Listeria monocytogenes, 13 (12.62%) by Listeria innocua, 6 (5.83%) by Listeria grayi, and 1 (0.97%) by Listeria welshimeri. A higher incidence of L. monocytogenes was observed mainly in the homemade Minas Frescal cheeses (a Brazilian soft white cheese, eaten fresh), 7 of 17 (41.17%), followed by ripened cheeses, 3 of 53 (5.67%), and industrially manufactured Frescal (Minas and Ricotta) cheeses, 1 of 33 (3.03%). Three serotypes (l/2a, l/2b and 4b) were observed among the strains of L. monocytogenes isolated, all of them being frequently involved in outbreaks of foodborne listeriosis and sporadic cases of the disease all over the world.

Effect of Ozone on the Inactivation of Yersinia enterocolitica and the Reduction of Natural Flora on Potatoes

2006 ◽  
Vol 69 (10) ◽  
pp. 2357-2363 ◽  
Author(s):  
MARÍA VICTORIA SELMA ◽  
DAVID BELTRÁN ◽  
ELISEO CHACÓN-VERA ◽  
MARÍA ISABEL GIL
Keyword(s):  
Listeria Monocytogenes ◽  
Yersinia Enterocolitica ◽  
Disease Outbreaks ◽  
Wash Water ◽  
Foodborne Disease ◽  
Pathogenic Microorganisms ◽  
Psychrotrophic Bacteria ◽  
Fresh Vegetables ◽  
Natural Flora ◽  
Soil Irrigation

Fresh vegetables contaminated with Yersinia enterocolitica have been implicated in foodborne disease outbreaks. Surfaces of vegetables can become contaminated with pathogenic microorganisms through contact with soil, irrigation water, fertilizers, equipment, humans, and animals. One approach to reduce this contamination is to treat fresh produce with sanitizers. In this study, the ability of ozone to inactivate Y. enterocolitica inoculated in water and on potato surfaces was evaluated. Furthermore, the efficacy of ozone in reducing natural flora on whole potato was determined. Total aerobic mesophilic and psychrotrophic bacteria, total coliforms, and Listeria monocytogenes were enumerated. Finally, several disinfection kinetic models were considered to predict Y. enterocolitica inactivation with ozone. Treatments with ozone (1.4 and 1.9 ppm) for 1 min decreased the Y. enterocolitica population in water by 4.6 and 6.2 log CFU ml−1, respectively. Furthermore, ozonated water (5 ppm) for 1 min decreased Y. enterocolitica and L. monocytogenes from potato surfaces by 1.6 and 0.8 log CFU g−1, respectively. Therefore, ozone can be an effective treatment for disinfection of wash water and for reduction of potato surface contamination.

scholarly journals Thermal inactivation and growth potential of Listeria monocytogenes in smoked tench

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Raffaella Branciari ◽  
Andrea Valiani ◽  
Raffaella Franceschini ◽  
David Ranucci ◽  
Alessia Lupattelli ◽  
...  
Keyword(s):  
Experimental Study ◽  
Listeria Monocytogenes ◽  
Thermal Inactivation ◽  
Challenge Test ◽  
Listeria Innocua ◽  
Growth Potential ◽  
Post Processing ◽  
Colony Forming Unit ◽  
Post Process ◽  
Hygiene Measures

An experimental study for the evaluation of <em>Listeria monocytogenes</em> inactivation during a hot smoking process in tench was performed using <em>Listeria innocua</em> strains. Furthermore, the survival of <em>L. monocytogenes</em> in smoked tench was determined after post-processing in contaminated samples, evaluating the growth potential during storage. <em>L. innocua</em> was not detected after the smoking process. In the challenge test, the growth potential of <em>L. monocytogenes</em> was 5.68 log colony forming unit g<sup>−1</sup>. The results showed that hot smoking at an inner temperature around 72°C is able to eliminate the microorganism. Nevertheless, the product is able to support the growth of the pathogen if post-process contamination occurs, as the food is suitable for <em>Listeria</em> multiplication. Product recontamination should be prevented by means of appropriate application of hygiene measures.

Gene Transcription Patterns of pH- and Salt-Stressed Listeria monocytogenes Cells in Simulated Gastric and Pancreatic Conditions

2014 ◽  
Vol 77 (2) ◽  
pp. 254-261 ◽  
Author(s):  
MARIOS MATARAGAS ◽  
ANNA GREPPI ◽  
KALLIOPI RANTSIOU ◽  
LUCA COCOLIN
Keyword(s):  
Life Cycle ◽  
Listeria Monocytogenes ◽  
Reference Strain ◽  
Expression Patterns ◽  
Wild Strain ◽  
Hostile Environment ◽  
Fermented Sausage ◽  
Laboratory Reference ◽  
Serotype 1 ◽  
Serotype 4B

A Listeria monocytogenes subgenomic array, targeting 54 genes involved in the adhesion, adaptation, intracellular life cycle, invasion, and regulation of the infection cycle was used to investigate the gene expression patterns of acid- and salt-stressed Listeria cells after exposure to conditions similar to those in gastric and pancreatic fluids. Three L. monocytogenes strains, one laboratory reference strain (EGDe) and two food isolates (wild strain 12 isolated from milk and wild strain 3 isolated from fermented sausage), were used during the studies. Differences in the expressed genes were observed between the gastric and pancreatic treatments and also between the serotypes. Increased transcripts were observed of the genes belonging to the adaptation and regulation group for serotype 4b (strain 12) and to the invasion and regulation group for serotype 1/2a (strain EGDe). Interestingly, no significantly differentially expressed genes were found for serotype 3c (strain 3) in most cases. The genes related to adaptation (serotype 1/2a) and to intracellular life cycle and invasion (serotype 4b) were down-regulated in order to cope with the hostile environment of the gastric and pancreatic fluids. These findings may provide experimental evidence for the dominance of serotypes 1/2a and 4b in clinical cases of listeriosis and for the sporadic occurrence of serotype 3c.

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