Incidence of Listeria monocytogenes in Raw Seafood Products in Japanese Retail Stores

2005 ◽  
Vol 68 (2) ◽  
pp. 411-415 ◽  
Author(s):  
SATOKO HANDA ◽  
BON KIMURA ◽  
HAJIME TAKAHASHI ◽  
TAKASHI KODA ◽  
KAZUO HISA ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Retail Stores ◽  
Serotype 1 ◽  
Seafood Products ◽  
Raw Fish ◽  
Fish Roe

The incidence of Listeria monocytogenes in raw fish, shellfish, and fish roe was investigated in seafood products collected from randomly selected retail stores in and around Tokyo, Japan. Of the 10 samples of 208 examined found positive for L. monocytogenes by mini-VIDAS LMO, seven were fish roe (cod, salmon) and three were minced tuna. Three serotypes (1/2a, 1/2b, 3b) were detected among the isolated strains; serotype 1/2a was predominant (8 of 10).

Recovery and Serotype Distribution of Listeria monocytogenes from Broiler Chickens in the Southeastern United States

1989 ◽  
Vol 52 (3) ◽  
pp. 148-150 ◽  
Author(s):  
J. S. BAILEY ◽  
D. L. FLETCHER ◽  
N. A. COX
Keyword(s):  
United States ◽  
Listeria Monocytogenes ◽  
Broiler Chickens ◽  
Southeastern United States ◽  
Retail Stores ◽  
Serotype Distribution ◽  
Epidemiological Evidence ◽  
Listeria Species ◽  
Processing Plants ◽  
Serotype 1

A total of ninety broiler carcasses from three processing plants were obtained from retail stores in the southeastern United States. The optimum plating medium was determined and carcasses were rinse sampled and the recovery of Listeria monocytogenes and other Listeria species determined. Presumptive L. monocytogenes isolates were serotyped. Listeria were recovered from 34 of 90 (38%) of the carcasses sampled, while L. monocytogenes were recovered from 21 of 90 (23%) of the carcasses sampled. Of the 35 L. monocytogenes isolates serotyped, 21 (64%) were confirmed to be serotype 1/2 b and 6 (18%) were confirmed to be serotype 1/2 c. Although there is no epidemiological evidence to suggest a relationship between consumption of chicken and listeriosis, the presence of L. monocytogenes on 23% of sampled broilers emphasizes the importance of maintaining hygienic practices in production, processing and preparation of fresh broilers.

Incidence and Recovery of Listeria from Chicken with a Pre-enrichment Technique

1990 ◽  
Vol 53 (7) ◽  
pp. 555-557 ◽  
Author(s):  
Y. VARABIOFF
Keyword(s):  
Listeria Monocytogenes ◽  
Water Samples ◽  
Listeria Innocua ◽  
Wash Water ◽  
Retail Stores ◽  
Enrichment Technique ◽  
Enrichment Procedure ◽  
Serotype 1

Eighty frozen chickens from four processors were purchased from retail stores in Brisbane. Forty-eight fresh chicken carcasses and 32 (16 hot and 16 chilled) wash-water samples from each of the four processors were also collected. The isolation of Listeria was achieved by a pre-enrichment procedure which allowed the recovery of injured cells. Listeria monocytogenes was isolated from 12 (15%) of the frozen chickens. Nine (11.2%) isolates were confirmed to be serotype 1 and three (3.8%) serotype 4. Fourteen (17.5%) of the frozen chickens were also contaminated by Listeria innocua. One (2.1%) sample of the fresh chickens yielded L. monocytogenes serotype 1 and five (10.4%) had L. innocua. L. monocytogenes serotype 1 was recovered from two (6.2%) samples of chilled wash water, but no Listeria were detected in hot wash water.

scholarly journals Risk of Listeria monocytogenes Contamination of Raw Ready-To-Eat Seafood Products Available at Retail Outlets in Japan

2010 ◽  
Vol 76 (10) ◽  
pp. 3383-3386 ◽  
Author(s):  
Satoko Miya ◽  
Hajime Takahashi ◽  
Tatsuya Ishikawa ◽  
Tateo Fujii ◽  
Bon Kimura
Keyword(s):  
Listeria Monocytogenes ◽  
Most Probable Number ◽  
Infection Model ◽  
Probable Number ◽  
Cell Numbers ◽  
Regulatory Guidelines ◽  
Retail Outlets ◽  
Seafood Products ◽  
Storage Temperatures ◽  
Fish Roe

ABSTRACT Examination of Listeria monocytogenes prevalence among ready-to-eat foods in Japan revealed frequent (5.7 to 12.1%) contamination of minced tuna and fish roe products, and the isolates had the same virulence levels as clinical isolates in terms of invasion efficiency and infectivity in cell cultures and a murine infection model, respectively. Premature stop codons in inlA were infrequent (1 out of 39 isolates). Cell numbers of L. monocytogenes in minced tuna and salmon roe increased rapidly under inappropriate storage temperatures (from a most probable number [MPN] of 100 to 101/g to an MPN of 103 to 104/g over the course of 2 days at 10°C). Thus, regulatory guidelines are needed for acceptable levels of L. monocytogenes in these foods.

Listeria in Seafoods: A Review

1992 ◽  
Vol 55 (12) ◽  
pp. 1009-1015 ◽  
Author(s):  
RONDA M. DILLON ◽  
THAKOR R. PATEL
Keyword(s):  
Listeria Monocytogenes ◽  
Heat Resistance ◽  
Drug Administration ◽  
Food And Drug Administration ◽  
Environmental Contaminant ◽  
Fresh Waters ◽  
Smoked Fish ◽  
Seafood Products ◽  
Crab Meat

Listeria is an environmental contaminant which has been isolated from marine and fresh waters, as well as various seafoods. Furthermore, Listeria, including Listeria monocytogenes, has been isolated from processed seafood products such as smoked fish, cooked and frozen seafoods, marinated fish, surimi products, etc. The pathogen, L. monocytogenes, does have a certain degree of heat resistance. It was found to survive in internally infected shrimp after boiled for up to 5 min. However, the commercial pasteurization process for crab meat was found to be sufficient to inactivate Listeria. The current recovery methodology for L. monocytogenes from seafoods is the Food and Drug Administration Listeria protocol.

Gene Transcription Patterns of pH- and Salt-Stressed Listeria monocytogenes Cells in Simulated Gastric and Pancreatic Conditions

2014 ◽  
Vol 77 (2) ◽  
pp. 254-261 ◽  
Author(s):  
MARIOS MATARAGAS ◽  
ANNA GREPPI ◽  
KALLIOPI RANTSIOU ◽  
LUCA COCOLIN
Keyword(s):  
Life Cycle ◽  
Listeria Monocytogenes ◽  
Reference Strain ◽  
Expression Patterns ◽  
Wild Strain ◽  
Hostile Environment ◽  
Fermented Sausage ◽  
Laboratory Reference ◽  
Serotype 1 ◽  
Serotype 4B

A Listeria monocytogenes subgenomic array, targeting 54 genes involved in the adhesion, adaptation, intracellular life cycle, invasion, and regulation of the infection cycle was used to investigate the gene expression patterns of acid- and salt-stressed Listeria cells after exposure to conditions similar to those in gastric and pancreatic fluids. Three L. monocytogenes strains, one laboratory reference strain (EGDe) and two food isolates (wild strain 12 isolated from milk and wild strain 3 isolated from fermented sausage), were used during the studies. Differences in the expressed genes were observed between the gastric and pancreatic treatments and also between the serotypes. Increased transcripts were observed of the genes belonging to the adaptation and regulation group for serotype 4b (strain 12) and to the invasion and regulation group for serotype 1/2a (strain EGDe). Interestingly, no significantly differentially expressed genes were found for serotype 3c (strain 3) in most cases. The genes related to adaptation (serotype 1/2a) and to intracellular life cycle and invasion (serotype 4b) were down-regulated in order to cope with the hostile environment of the gastric and pancreatic fluids. These findings may provide experimental evidence for the dominance of serotypes 1/2a and 4b in clinical cases of listeriosis and for the sporadic occurrence of serotype 3c.

scholarly journals Sources of Listeria monocytogenes Contamination in a Cold-Smoked Rainbow Trout Processing Plant Detected by Pulsed-Field Gel Electrophoresis Typing

1999 ◽  
Vol 65 (1) ◽  
pp. 150-155 ◽  
Author(s):  
Tiina Autio ◽  
Sebastian Hielm ◽  
Maria Miettinen ◽  
Anna-Maija Sjöberg ◽  
Kaarina Aarnisalo ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Hot Water ◽  
Processing Plant ◽  
Pulsed Field ◽  
Eradication Program ◽  
Fish Samples ◽  
Raw Fish

ABSTRACT Sites of Listeria monocytogenes contamination in a cold-smoked rainbow trout (Oncorhynchus mykiss) processing plant were detected by sampling the production line, environment, and fish at different production stages. Two lots were monitored. The frequency of raw fish samples containing L. monocytogenes was low. During processing, the frequency of fish contaminated with L. monocytogenes clearly rose after brining, and the most contaminated sites of the processing plant were the brining and postbrining areas. A total of 303 isolates from the raw fish, product, and the environment were characterized by pulsed-field gel electrophoresis (PFGE). PFGE yielded nine pulsotypes, which formed four clusters. The predominating L. monocytogenespulsotypes of the final product were associated with brining and slicing, whereas contaminants of raw fish were not detected in the final product. Air-mediated contamination in the plant could not be proved. In accordance with these results, an L. monocytogenes eradication program was planned. The use of hot steam, hot air, and hot water seemed to be useful in eliminatingL. monocytogenes. None of the control samples taken in the 5 months after the eradication program was implemented containedL. monocytogenes.

scholarly journals Genome Sequence of theListeria monocytogenesFood Isolate HPB913, Collected in Canada in 1993

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Arthur W. Pightling ◽  
Hugh Rand ◽  
Errol Strain ◽  
Franco Pagotto
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
Food Safety ◽  
Genome Sequence ◽  
Sporadic Case ◽  
Foodborne Illness ◽  
Pathogenic Bacterium ◽  
Food Isolate ◽  
Serotype 1

Listeria monocytogenesis a pathogenic bacterium of importance to public health and food safety agencies. We present the genome sequence of the serotype 1/2aL. monocytogenesfood isolate HPB913, which was collected in Canada in 1993 as part of an investigation into a sporadic case of foodborne illness.

scholarly journals Construction, Characterization, and Use of Two Listeria monocytogenes Site-Specific Phage Integration Vectors

2002 ◽  
Vol 184 (15) ◽  
pp. 4177-4186 ◽  
Author(s):  
Peter Lauer ◽  
Man Yin Nora Chow ◽  
Martin J. Loessner ◽  
Daniel A. Portnoy ◽  
Richard Calendar
Keyword(s):  
Listeria Monocytogenes ◽  
Lethal Dose ◽  
Attachment Site ◽  
Donor Cell ◽  
Single Copy ◽  
Cell Extract ◽  
Site Specific ◽  
Specific Phage ◽  
Serotype 1 ◽  
Serotype 4B

ABSTRACT Two site-specific shuttle integration vectors were developed with two different chromosomal bacteriophage integration sites to facilitate strain construction in Listeria monocytogenes. The first vector, pPL1, utilizes the listeriophage U153 integrase and attachment site within the comK gene for chromosomal insertion. pPL1 contains a useful polylinker, can be directly conjugated from Escherichia coli into L. monocytogenes, forms stable, single-copy integrants at a frequency of ∼10−4 per donor cell, and can be used in the L. monocytogenes 1/2 and 4b serogroups. Methods for curing endogenous prophages from the comK attachment site in 10403S-derived strains were developed. pPL1 was used to introduce the hly and actA genes at comK-attBB′ in deletion strains derived from 10403S and SLCC-5764. These strains were tested for second-site complementation in hemolysin assays, plaquing assays, and cell extract motility assays. Unlike plasmid-complemented strains, integrated pPL1-complemented strains were fully virulent in the mouse 50% lethal dose assay. Additionally, the PSA phage attachment site on the L. monocytogenes chromosome was characterized, and pPL1 was modified to integrate at this site. The listeriophage PSA integrates in the 3′ end of an arginine tRNA gene. There are 17 bp of DNA identity between the bacterial and phage attachment sites. The PSA prophage DNA sequence reconstitutes a complete tRNAArg gene. The modified vector, pPL2, was integration proficient at the same frequency as pPL1 in common laboratory serotype 1/2 strains as well as serotype 4b strains.

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