Rapid Method for Prediction of Escherichia coli Numbers Using an Electronic Sensor Array and an Artificial Neural Network

2004 ◽  
Vol 67 (8) ◽  
pp. 1604-1609 ◽  
Author(s):  
UBONRATANA SIRIPATRAWAN ◽  
JOHN E. LINZ ◽  
BRUCE R. HARTE

An electronic sensor array with 12 nonspecific metal oxide sensors was evaluated for its ability to monitor volatile compounds in super broth alone and in super broth inoculated with Escherichia coli (ATCC 25922) at 37°C for 2 to 12 h. Using discriminant function analysis, it was possible to differentiate super broth alone from that containing E. coli when cell numbers were 105 CFU or more. There was a good agreement between the volatile profiles from the electronic sensor array and a gas chromatography–mass spectrometer method. The potential to predict the number of E. coli and the concentration of specific metabolic compounds was investigated using an artificial neural network (ANN). The artificial neural network was composed of an input layer, one hidden layer, and an output layer, with a hyperbolic tangent sigmoidal transfer function in the hidden layer and a linear transfer function in the output layer. Good prediction was found as measured by a regression coefficient (R2 = 0.999) between actual and predicted data.

2006 ◽  
Vol 69 (8) ◽  
pp. 1844-1850 ◽  
Author(s):  
UBONRAT SIRIPATRAWAN ◽  
JOHN E. LINZ ◽  
BRUCE R. HARTE

A rapid method for the detection of Escherichia coli (ATCC 25922) in packaged alfalfa sprouts was developed. Volatile compounds from the headspace of packaged alfalfa sprouts, inoculated with E. coli and incubated at 10°C for 1, 2, and 3 days, were collected and analyzed. Uninoculated sprouts were used as control samples. An electronic nose with 12 metal oxide electronic sensors was used to monitor changes in the composition of the gas phase of the package headspace with respect to volatile metabolites produced by E. coli. The electronic nose was able to differentiate between samples with and without E. coli. To predict the number of E. coli in packaged alfalfa sprouts, an artificial neural network was used, which included an input layer, a hidden layer, and an output layer, with a hyperbolic tangent sigmoidal transfer function in the hidden layer and a linear transfer function in the output layer. The network was shown to be capable of correlating voltametric responses with the number of E. coli. A good prediction was possible, as measured by a regression coefficient (R2 = 0.903) between the actual and predicted data. In conjunction with the artificial neural network, the electronic nose proved to have the ability to detect E. coli in packaged alfalfa sprouts.


2018 ◽  
Vol 204 ◽  
pp. 02018
Author(s):  
Aisyah Larasati ◽  
Anik Dwiastutik ◽  
Darin Ramadhanti ◽  
Aal Mahardika

This study aims to explore the effect of kurtosis level of the data in the output layer on the accuracy of artificial neural network predictive models. The artificial neural network predictive models are comprised of one node in the output layer and six nodes in the input layer. The number of hidden layer is automatically built by the program. Data are generated using simulation approach. The results show that the kurtosis level of the node in the output layer is significantly affect the accuracy of the artificial neural network predictive model. Platycurtic and leptocurtic data has significantly higher misclassification rates than mesocurtic data. However, the misclassification rates between platycurtic and leptocurtic is not significantly different. Thus, data distribution with kurtosis nearly to zero results in a better ANN predictive model.


2008 ◽  
Vol 59 (10) ◽  
Author(s):  
Gozde Pektas ◽  
Erdal Dinc ◽  
Dumitru Baleanu

Simultaneaous spectrophotometric determination of clorsulon (CLO) and invermectin (IVE) in commercial veterinary formulation was performed by using the artificial neural network (ANN) based on the back propagation algorithm. In order to find the optimal ANN model various topogical networks were tested by using different hidden layers. A logsig input layer, a hidden layer of neurons using the logsig transfer function and an output layer of two neurons with purelin transfer function was found suitable for basic configuration for ANN model. A calibration set consisting of CLO and IVE in calibration set was prepared in the concentration range of 1-23 �g/mL and 1-14 �g/mL, repectively. This calibration set contains 36 different synthetic mixtures. A prediction set was prepared in order to evaluate the recovery of the investigated approach ANN chemometric calibration was applied to the simultaneous analysis of CLO and IVE in compounds in a commercial veterinary formulation. The experimental results indicate that the proposed method is appropriate for the routine quality control of the above mentioned active compounds.


2018 ◽  
Vol 1 (1) ◽  
pp. 10
Author(s):  
Habibi Ratu Perwira Negara ◽  
Irzani Irzani ◽  
Ripai Ripai

Abstrak: Penelitian ini bertujuan untuk menentukan model pola curah hujan menggunakan Artificial Neural Network (ANN) dengan metode Backpropagatiaon. Sampel dalam penelitian ini adalah 5 pos pencataan hujan di daerah Lombok Tengah bagian Selatan dan 2 pos pencatatan hujan di  daerah Lombok Timur bagian Selatan. Data penelitian berupa data setengah bulanan yang dicatat dari tahun 1973 sampai tahun 2010 untuk daerah Lombok Tengah bagian Selatan dan data dari tahun 1974 sampai tahun 2010 untuk daerah Lombok Timur bagian Selatan. Metode penilitian dilakukan dengan melakukan pembelajaran data curah hujan menggunakan 5 arsitektur yang berbeda. Pembelajaran dilakukan untuk menemukan arsitektur terbaik. Arsitektur untuk daerah Lombok Tengah bagian Selatan adalah 120 layer inputan, 240 layer hidden 1, 12 layer hidden 2, dan 1 layer output. Sedangkan arsitektur untuk daerah Lombok Timur bagian Selatan adalah 363 layer inputan, 54 layer hidden 1, 24 layer hidden 2, dan 1 layer output. Model matematika pola curah hujan yang diperoleh adalah .Abstract:  This study aims to determine the model of rainfall pattern using Artificial Neural Network (ANN) with Backpropagatiaon method. The sample in this research is 5 rainfall checkpoint in South Central Lombok and 2 post recording of rain in South East of Lombok. The research data are semi-monthly data recorded from 1973 to 2010 for the southern part of Central Lombok and data from 1974 to 2010 for the southern part of Lombok Timur. Methods of research conducted by conducting rainfall data learning using 5 different architectures. Learning is done to find the best architecture. The architecture for the Southern Central Lombok area is 120 layers of input, 240 hidden layers 1, 12 hidden layers 2, and 1 output layer. While the architecture for the southern part of East Lombok is 363 layers inputan, 54 hidden layer 1, 24 hidden layer 2, and 1 output layer. The mathematical model of the obtained rainfall pattern is .


Author(s):  
Aseel Shakir I. Hilaiwah ◽  
Hanan Abed Alwally Abed Allah ◽  
Basim Akhudir Abbas ◽  
Tole Sutikno

<span>An extensive review of the artificial neural network (ANN) is presented in this paper. Previous studies review the artificial neural network (ANN) based on the approaches (algorithms) used or based on the types of the artificial neural network (ANN). The presented paper reviews the ANN based on the goal of the ANN (methods, and layers), which become the main objective of this paper. As a famous artificial intelligent model, ANN mimics the human nervous system in handling the information transmited by different nodes (also known as neurons) in this model. These nodes are stacked in layers and work collectively to bring about solution to complex problems. Numerous structures exist for ANN and each of these structures is designed to addressa a specific task. Basically, the ANN architecture is comprised of 3 different layers wherein the first layer rpresents the input layer that consist of several input nodes that represent the input parameterfor the model. The hidden layer is te second layer and consists of a hidden layer of neurons. The neurons in this layer are directly connected to the neurons in the output layer. The third layer is the output layer which is the models’ response layer. The output layer neurons have the activation functions for the calculation of the ANN final output. The connection between the nodes in the ANN model is mediated by synaptic weights. This paper is a comprehensive study of ANN models and their layers.</span>


Author(s):  
Prakash Chandra Mishra ◽  
Anil Kumar Giri

Artificial neural network model is applied for the prediction of the biosorption capacity of living cells of Bacillus cereus for the removal of chromium (VI) ions from aqueous solution. The maximum biosorption capacity of living cells of Bacillus cereus for chromium (VI) was found to be 89.24% at pH 7.5, equilibrium time of 60 min, biomass dosage of 6 g/L, and temperature of 30 ± 2 °C. The biosorption data of chromium (VI) ions collected from laboratory scale experimental set up is used to train a back propagation (BP) learning algorithm having 4-7-1 architecture. The model uses tangent sigmoid transfer function at input to hidden layer whereas a linear transfer function is used at output layer. The data is divided into training (75%) and testing (25%) sets. Comparison between the model results and experimental data gives a high degree of correlation R2 = 0.984 indicating that the model is able to predict the sorption efficiency with reasonable accuracy. Bacillus cereus biomass is characterized using AFM and FTIR.


2018 ◽  
Vol 1 (1) ◽  
pp. 65
Author(s):  
Dženana Sarajlić ◽  
Layla Abdel-Ilah ◽  
Adnan Fojnica ◽  
Ahmed Osmanović

This paper presents development of Artificial Neural Network (ANN) for prediction of the size of nanoparticles (NP) and microspore surface area (MSA). Developed neural network architecture has the following three inputs: the concentration of the biodegradable polymer in the organic phase, surfactant concentration in the aqueous phase and the homogenizing pressure. Two-layer feedforward network with a sigmoid transfer function in the hidden layer and a linear transfer function in the output layer is trained, using Levenberg-Marquardt training algorithm. For training of this network, as well as for subsequent validation, 36 samples were used. From 36 samples which were used for subsequent validation in this ANN, 80,5% of them had highest accuracy while 19,5% of output data had insignificant differences comparing to experimental values.


Author(s):  
Syukri Syukri ◽  
Samsuddin Samsuddin

<span lang="EN-US">Angin memiliki peran yang penting dalam kehidupan manusia, antara lain pada pembangkit listrik, pelayaran dan penerbangan. Ketiga sektor tersebut erat kaitannya dengan  kondisi angin. Angin dapat muncul setiap saat dan setiap waktu serta perubahan geografis pada suatu wilayah. Hal ini mengakibatkan sulitnya menentukan kecepatan angin, maka untuk mengatasi masalah tersebut diperlukan prediksi kecepatan angin. Saat ini berbagai metode prediksi telah banyak dikembangkan, salah satu metode yang dapat digunakan untuk melakukan prediksi dengan akurasi yang tinggi yaitu algoritma <em>Artificial Neural Network</em> (ANN) <em>Backpropagation</em>. Arsitektur ANN yang digunakan adalah  4 parameter <em>input layer</em>, <em>hidden layer</em> (5, 10, 15, 20, 25 dan 30) dan <em>output layer</em> (1 parameter). Data pembelajaran dan pengujian didapatkan dari stasiun BMKG Blang Bintang Aceh Besar, berupa data kecepatan angin jam per harian periode Januari 2011 sampai dengan Desember 2015 yang terdiri dari arah angin, suhu, tekanan, kelembaban dan suhu. Hasil pengujian menunjukkan bahwa metode ANN <em>Backpropagation </em>cukup baik diterapkan untuk proses prediksi, kemampuan ANN dalam melakukan prediksi memiliki tingkat akurasi rata – rata yang lebih baik yaitu 96 %. Sedangkan nilai rata – rata kerapatan daya angin jam per harian yaitu </span><span lang="EN-US">45.030 W/m<sup>2</sup></span>


Author(s):  
Prakash Chandra Mishra ◽  
Anil Kumar Giri

Artificial neural network model is applied for the prediction of the biosorption capacity of living cells of Bacillus cereus for the removal of chromium (VI) ions from aqueous solution. The maximum biosorption capacity of living cells of Bacillus cereus for chromium (VI) was found to be 89.24% at pH 7.5, equilibrium time of 60 min, biomass dosage of 6 g/L, and temperature of 30 ± 2 °C. The biosorption data of chromium (VI) ions collected from laboratory scale experimental set up is used to train a back propagation (BP) learning algorithm having 4-7-1 architecture. The model uses tangent sigmoid transfer function at input to hidden layer whereas a linear transfer function is used at output layer. The data is divided into training (75%) and testing (25%) sets. Comparison between the model results and experimental data gives a high degree of correlation R2 = 0.984 indicating that the model is able to predict the sorption efficiency with reasonable accuracy. Bacillus cereus biomass is characterized using AFM and FTIR.


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