Occurrence of Campylobacter and Listeria monocytogenes in a Poultry Processing Plant

2005 ◽  
Vol 68 (9) ◽  
pp. 1903-1906 ◽  
Author(s):  
MERCEDES G. R. REITER ◽  
CAMILA M. M. BUENO ◽  
CARMEN LÓPEZ ◽  
R. JORDANO
Keyword(s):  
Listeria Monocytogenes ◽  
Processing Plant ◽  
Southern Brazil ◽  
Poultry Products ◽  
Sampling Points ◽  
Poultry Processing ◽  
Conventional Methods

The occurrence of Campylobacter and Listeria monocytogenes was studied in 645 samples from surfaces, water, and poultry products (chicken carcasses, chicken parts, viscera, and spoils) in a poultry processing plant in southern Brazil. The automated mini-VIDAS system was used to detect the presence of Campylobacter and L. monocytogenes on the samples. The positive samples were confirmed by conventional methods. Campylobacter and L. monocytogenes were found in 16.6 and 35.6% of the analyzed samples, respectively. The sampling points with the highest Campylobacter incidence were intestine (63.3%, 19 of 30 samples), gallbladder (46.7%, 14 of 30), carcasses before evisceration (33.33%, 10 of 30), and carcasses after plucking (30%, 9 of 30). For L. monocytogenes, the majority of positive samples were from frozen breast (100%, 15 of 15 samples), frozen wing (93.3%, 14 of 15), fresh breast (83.3%, 25 of 30), fresh wing (80%, 24 of 30), skin of breast and leg (76.7%, 23 of 30), frozen leg (60%, 9 of 15), and fresh leg (50%, 15 of 30).

Reduction by Competitive Bacteria of Listeria monocytogenes in Biofilms and Listeria Bacteria in Floor Drains in a Ready-to-Eat Poultry Processing Plant

2013 ◽  
Vol 76 (4) ◽  
pp. 601-607 ◽  
Author(s):  
TONG ZHAO ◽  
TERESA C. PODTBURG ◽  
PING ZHAO ◽  
DONG CHEN ◽  
DAVID A. BAKER ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Listeria Monocytogenes ◽  
Competitive Exclusion ◽  
Processing Plant ◽  
Enterococcus Durans ◽  
Poultry Products ◽  
Poultry Processing ◽  
Lactis Strain ◽  
Scanning Electron

The ability of Listeria monocytogenes and two competitive exclusion (CE) bacteria, Lactococcus lactis subsp. lactis strain C-1-92 and Enterococcus durans strain 152, to form biofilms on coupons composed of different materials (stainless steel, plastic, rubber, glass, and silicone) was determined at 4 and 8°C. Biofilm characteristics were determined by scanning electron microscopy. L. monocytogenes produced well-formed biofilms within 24 h at 37°C on coupon surfaces. Treating Listeria-laden biofilms with the CE isolates individually at either 4 or 8°C for 3 weeks substantially reduced or eliminated listeriae in the biofilms. Treatment with L. lactis subsp. lactis strain C-1-92 and E. durans strain 152 at 4°C for 3 weeks reduced the population of L. monocytogenes in a biofilm from 7.1 to 7.7 log CFU/cm2 to 3.0 to 4.5 log CFU/cm2 and to 3.1 to 5.2 log CFU/cm2, respectively, and treatment at 8°C for 3 weeks reduced L. monocytogenes from 7.5 to 8.3 log CFU/cm2 to 2.4 to 3.5 log CFU/cm2 and to 3.8 to 5.2 log CFU/cm2, respectively, depending on the coupon composition. These two CE isolates were combined and evaluated for control of Listeria bacteria in floor drains of a ready-to-eat poultry processing plant. The results revealed that treating the floor drains with CE four times in the first week eliminated detectable Listeria bacteria from five of six drains, and the drains remained free of detectable Listeria bacteria for 13 weeks following the first four treatments. These studies indicate that CE can effectively reduce Listeria contamination in biofilms and in flow drains of a plant producing ready-to-eat poultry products.

Traceback Identification of an Ingredient (Pork Dewlap) as the Possible Source of Listeria monocytogenes Serotype 4b Contamination in Raw Chicken Products

2007 ◽  
Vol 70 (6) ◽  
pp. 1513-1517 ◽  
Author(s):  
VICTORIA LÓPEZ ◽  
SAGRARIO ORTIZ ◽  
ALFREDO CORUJO ◽  
PILAR LÓPEZ ◽  
JAIME NAVAS ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Restriction Analysis ◽  
Virulence Gene ◽  
Pulsed Field Gel Electrophoresis ◽  
Processing Plant ◽  
Poultry Processing ◽  
Serotype 4B ◽  
Fresh Products ◽  
Allelic Analysis

In surveys conducted on finished product samples from a single poultry processing plant in Spain, Listeria monocytogenes was found in 14 different uncooked products. To track contamination patterns, 77 L. monocytogenes isolates were characterized by PCR-based serotyping, pulsed-field gel electrophoresis (PFGE) restriction analysis, and PCR-based allelic analysis of the virulence gene actA. Serotyping revealed that 12 isolates (15.6%) were of the L. monocytogenes serotype 4b complex (serotype 4b or the closely related serotypes 4d and 4e). A combination of endonucleases AscI and ApaI PFGE patterns yielded 15 different pulsotypes among all 77 tested isolates. All the serotype 4b isolates belonged to one pulsotype. Sequencing of the actA gene confirmed that all serotype 4b isolates corresponded to the same allelic subtype. The subtype was recovered from five product types, but its presence was not correlated with the production line or the date of isolation, suggesting a possible association of this strain with a common ingredient. This traceback investigation established that pork dewlap, an ingredient common to all the products contaminated with this strain, was the most probable source of L. monocytogenes 4b. The same 4b strain was isolated from four samples of pork dewlap from one specific supplier. After replacement of this contaminated ingredient in the fresh products, this strain of L. monocytogenes serotype 4b was not detected. This study confirms the effectiveness of molecular subtyping to control contamination by specific strains of L. monocytogenes and the importance of testing the different ingredients added to the food products.

Controlling Attachment and Growth of Listeria monocytogenes in Polyvinyl Chloride Model Floor Drains Using a Peroxide Chemical, Chitosan-Arginine, or Heat†

2014 ◽  
Vol 77 (12) ◽  
pp. 2129-2132 ◽  
Author(s):  
MARK E. BERRANG ◽  
CHARLES L. HOFACRE ◽  
JOSEPH F. FRANK
Keyword(s):  
Listeria Monocytogenes ◽  
Polyvinyl Chloride ◽  
Hot Water ◽  
Plate Count ◽  
Processing Plant ◽  
Peroxyacetic Acid ◽  
Planktonic Cells ◽  
Log Reduction ◽  
Before And After ◽  
Poultry Processing

Listeria monocytogenes can colonize a poultry processing plant as a resident in floor drains. Limiting growth and attachment to drain surfaces may help lessen the potential for cross-contamination of product. The objective of this study was to compare a hydrogen peroxide-peroxyacetic acid–based chemical to chitosan-arginine or heat to prevent attachment of or destroy existing L. monocytogenes on the inner surface of model floor drains. L. monocytogenes was introduced to result in about 109 planktonic and attached cells within untreated polyvinyl chloride model drain pipes. Treatments (0.13% peroxide-based sanitizer, 0.1% chitosan-arginine, or 15 s of hot water at 95 to 100°C) were applied immediately after inoculation or after 24 h of incubation. Following treatment, all pipes were incubated for an additional 24 h; planktonic and attached cells were enumerated by plate count. All treatments significantly (P < 0.05) lowered numbers of planktonic and attached cells recovered. Chitosan-arginine resulted in approximately a 6-log reduction in planktonic cells when applied prior to incubation and a 3-log reduction after the inoculum had a chance to grow. Both heat and peroxide significantly outperformed chitosan-arginine (8- to 9-log reduction) and were equally effective before and after incubation. Heat was the only treatment that eliminated planktonic L. monocytogenes. All treatments were less effective against attached cells. Chitosan-arginine provided about a 4.5-log decrease in attached cells when applied before incubation and no significant decrease when applied after growth. Like with planktonic cells, peroxide–peroxyacetic acid and heat were equally effective before or after incubation, causing decreases ranging from 7 to 8.5 log for attached L. monocytogenes. Applied at the most efficacious time, any of these techniques may lessen the potential for L. monocytogenes to remain as a long-term resident in processing plant floor drains.

Physical activity in shift workers of a poultry processing plant in southern Brazil

2012 ◽  
Vol 15 ◽  
pp. S237
Author(s):  
D. Tietzmann ◽  
A. Garcez ◽  
V. Paniz ◽  
R. Canuto ◽  
M. Olinto
Keyword(s):  
Physical Activity ◽  
Processing Plant ◽  
Southern Brazil ◽  
Shift Workers ◽  
Poultry Processing

Impact of Nightshift Work on Overweight and Abdominal Obesity Among Workers of a Poultry Processing Plant in Southern Brazil

2012 ◽  
Vol 29 (3) ◽  
pp. 336-343 ◽  
Author(s):  
Jamile Macagnan ◽  
Marcos Pascoal Pattussi ◽  
Raquel Canuto ◽  
Ruth Liane Henn ◽  
Anaclaudia Gastal Fassa ◽  
...  
Keyword(s):  
Abdominal Obesity ◽  
Processing Plant ◽  
Southern Brazil ◽  
Poultry Processing

Distribution and Persistence of Listeria monocytogenes in a Heavily Contaminated Poultry Processing Facility

2019 ◽  
Vol 82 (9) ◽  
pp. 1524-1531 ◽  
Author(s):  
BEATRIZ MELERO ◽  
BEATRIZ MANSO ◽  
BEATRIX STESSL ◽  
MARTA HERNÁNDEZ ◽  
MARTIN WAGNER ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Safety ◽  
Quality Management ◽  
Quality Management System ◽  
Food Samples ◽  
Processing Plant ◽  
Poultry Processing ◽  
Contact Surfaces ◽  
Food Safety And Quality ◽  
Sequence Types

ABSTRACT We studied the colonization and distribution of Listeria monocytogenes in a heavily contaminated poultry processing plant over a 1-year period. A total of 180 nonfood contact surfaces, 70 food contact surfaces, 29 personnel, and 40 food samples were analyzed. L. monocytogenes isolates were subtyped by PCR serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing. L. monocytogenes was detected in samples collected at every visit to the plant, and 43.8% (visit 4) to 65.6% (visit 7) of samples were positive, for an overall prevalence of 55.2%. The deboning area had the highest prevalence of positive samples (83.3%), and the processing area had the highest diversity of PFGE types. Ninety percent of the final products were positive for L. monocytogenes. Most of the isolates belonged to well-known persistent L. monocytogenes sequence types (ST9 and ST121). This study illustrates a well-established L. monocytogenes contamination problem in a poultry processing plant associated with a generalized failure of the food safety system as a whole. These findings reflect the potential for L. monocytogenes contamination when the food safety and quality management system is unsatisfactory, as described in the present study. It is essential to revise food safety and quality management systems to eliminate L. monocytogenes from food processing facilities, to control the entrance of sporadic sequence types, and to prevent L. monocytogenes spread within such facilities, especially in those premises with higher L. monocytogenes prevalence in the environment and final food products.

scholarly journals Persistent and Nonpersistent Listeria monocytogenes Contamination in Meat and Poultry Processing Plants

2003 ◽  
Vol 66 (11) ◽  
pp. 2062-2069 ◽  
Author(s):  
JANNE M. LUNDÉN ◽  
TIINA J. AUTIO ◽  
A.-M. SJÖBERG ◽  
HANNU J. KORKEALA
Keyword(s):  
Heat Treatment ◽  
Listeria Monocytogenes ◽  
Sampling Site ◽  
Pfge Type ◽  
Processing Plant ◽  
Post Heat Treatment ◽  
Common Location ◽  
Heat Treated ◽  
Processing Plants ◽  
Poultry Processing

Contamination analysis of persistent and nonpersistent Listeria monocytogenes strains in three meat processing plants and one poultry processing plant were performed in order to identify factors predisposing to or sustaining persistent plant contamination. A total of 596 L. monocytogenes isolates were divided into 47 pulsed-field gel electrophoresis (PFGE) types by combining the restriction enzyme patterns of AscI (42 patterns) and ApaI (38 patterns). Persistent and nonpersistent strains were found in all plants. Nonpersistent PFGE types were found mostly at one sampling site, with the processing environment being the most common location, whereas the persistent strains were found at several sampling sites in most cases. The processing machines were frequently contaminated with persistent L. monocytogenes PFGE types, and it was of concern that surfaces having direct contact with the products were contaminated. The role of the processing machines in sustaining contamination and in contaminating the products appeared to be important because the final product of several processing lines was contaminated with the same L. monocytogenes PFGE type as that found in the processing machine. The proportion of persistent PFGE types in heat-treated products was eight times higher than in the raw products, showing the importance of the persistent PFGE types as contaminants of the final heat-treated products. The contamination status of the processing lines and machines appeared to be influenced by the compartmentalization of the processing line, with poor compartmentalization increasing L. monocytogenes contamination. The separation of raw and post–heat treatment areas seemed especially important in the contamination status of post–heat treatment lines.

scholarly journals Job characteristics and musculoskeletal pain among shift workers of a poultry processing plant in Southern Brazil

2015 ◽  
Vol 57 (5) ◽  
pp. 448-456 ◽  
Author(s):  
Dânia Barro ◽  
Maria Teresa Anselmo Olinto ◽  
Jamile Block Araldi Macagnan ◽  
Ruth Liane Henn ◽  
Marcos Pascoal Pattussi ◽  
...  
Keyword(s):  
Musculoskeletal Pain ◽  
Job Characteristics ◽  
Processing Plant ◽  
Southern Brazil ◽  
Shift Workers ◽  
Poultry Processing
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