scholarly journals Streptococcus mutans and Streptococcus sobrinus detection by Polymerase Chain Reaction and their relation to dental caries in 12 and 15 year-old schoolchildren in Valencia (Spain)

2013 ◽  
pp. e839-e845 ◽  
Author(s):  
M. Sanchez-Acedo ◽  
JM. Montiel-Company ◽  
F. Dasi-Fernandez ◽  
JM. Almerich-Silla
Keyword(s):  
Polymerase Chain Reaction ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Streptococcus Sobrinus ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Effects of Caries Activity on Compositions of Mutans Streptococci in Saliva-Induced Biofilm Formed on Bracket Materials

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4764
Author(s):  
Bum-Soon Lim ◽  
Bo-Hyun Kim ◽  
Won-Jun Shon ◽  
Sug-Joon Ahn
Keyword(s):  
Polymerase Chain Reaction ◽  
Streptococcus Mutans ◽  
Mutans Streptococci ◽  
Growth Media ◽  
Streptococcus Sobrinus ◽  
Chain Reaction ◽  
Caries Activity ◽  
Material Type ◽  
Polymerase Chain ◽  
Total Bacteria

This study aimed to investigate effects of caries activity on composition of mutans streptococci in saliva-induced biofilms formed on bracket materials. Three bracket materials were used as specimens: ceramic, metal, and plastic. After saliva was collected using a spitting method from caries-active (CA, decayed, missing, filled teeth (DMFT) score ≥ 10) and caries-free (CF, DMFT score = 0) subjects, saliva was mixed with growth media in a proportion of 1:10. The saliva solution was then incubated with each bracket material. After a saliva-induced biofilm was developed on the surface of the bracket material, the amounts of total bacteria and mutans streptococci were determined using real-time polymerase chain reaction. The results showed that biofilms from CA saliva contained more mutans streptococci but less total bacteria than biofilms from CF saliva, regardless of material type. Adhesion of total bacteria to ceramic was higher than to plastic, regardless of caries activity. Mutans streptococci adhered more to ceramic than to metal and plastic in both biofilms from CA and CF saliva, but there was a difference in adhesion between Streptococcus mutans and Streptococcus sobrinus. The amount of S. mutans was higher than that of S. sobrinus in biofilms from CA saliva despite similar amounts of the two strains in biofilms from CF saliva. The stronger adhesion of S. mutans to ceramic than to metal and plastic was more evident in biofilms from CA saliva than in biofilms from CF saliva. This study suggests that caries activity and material type significantly influenced composition of mutans streptococci in biofilms formed on bracket materials.

scholarly journals Identifikasi gen kariogenik glukosiltransferase Streptococcus mutans pada pasien karies gigiIdentification of Streptococcus mutans cariogenic gene glucosyltransferase (gtf) in dental caries patients

2021 ◽  
Vol 33 (1) ◽  
pp. 14
Author(s):  
Rita Endriani ◽  
Fajri Marindra Siregar ◽  
Elita Rafni ◽  
Rahmat Kemal Azhari ◽  
Jefrizal Jefrizal
Keyword(s):  
Polymerase Chain Reaction ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Cellular Adhesion ◽  
Tooth Surface ◽  
Chain Reaction ◽  
Consecutive Sampling ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Attachment Process

Pendahuluan: Streptococcus mutans (S. mutans) merupakan bakteri yang mempunyai glukosiltransferase surface (GTFs) yang berperan pada proses  perlekatan dan virulensi bakteri pada permukaan gigi. Enzim GTFs terdiri atas  tiga jenis enzim GTFs yaitu GTFB, GTFC dan GTFD dan masing-masing enzim ini dikode oleh gen gtfB, gtfC dan gtfD yang juga berperan sebagai prekursor dalam adhesi seluler plak gigi yang dapat menyebabkan karies gigi. Tujuan penelitian adalah mengidentifikasi gen gtfC dan gtfD S. mutans  pada pasien karies gigi. Metode: Penelitian dilakukan dengan consecutive sampling. Sampel penelitian diambil dari kerokan jaringan karies gigi, diisolasi, dikultur, dan diidentifikasi di laboratorium Mikrobiologi dan Sentral FK UNRI. Identifikasi S. mutans dan gen kariogenik gtfC dan gtfD menggunakan metode Polymerase Chain Reaction (PCR). Data ditampilkan dalam bentuk tabel distribusi frekuensi dan dinyatakan dalam persentase Hasil: Dari 60 kerokan jaringan karies  didapatkan  S. mutans sebanyak 10 isolat, sedangkan gen gtfC dan gen gtfD didapatkan masing-masing 4 (40%) isolat. Simpulan: Gen kariogenik teridentifikasi gen gtfC dan gtfD pada isolat S. mutans dari pasien karies gigi sebanyak 40%.Kata kunci: gtfC, gtfD, kariogenik glukosiltransferase Streptococcus mutans. ABSTRACTIntroduction: Streptococcus mutans (S. mutans) is a bacterium with a glucosyltransferase (Gtfs) surface, which plays a role in the attachment process and bacteria virulence on the tooth surface. The Gtfs enzyme consists of three types of Gtfs enzymes, namely GtfB, GtfC, and GtfD. These enzymes are encoded by the GtfB, GtfC, and GtfD genes, which also act as precursors in the cellular adhesion of dental plaque that can cause dental caries. This study was aimed to identify the GtfC and GtfD of S. mutans in dental caries patients. Methods: This study was conducted with consecutive sampling. Research samples were taken from dental caries tissue scrapings, isolated, cultured, and identified in the Microbiology and Central Laboratory of the Faculty of Medicine University of Riau. Identification of S. mutans and GtfC and GtfD cariogenic genes using the Polymerase Chain Reaction (PCR) method. The data was displayed in the form of a frequency distribution table and expressed as a percentage. Results: From 60 caries tissue scrapings, ten isolates were obtained, while the GtfC gene and GtfD gene were obtained 4 (40%) isolates each. Conclusion: Forty percents of S. mutans isolates from dental caries patients were identified as the GtfC and GtfD cariogenic genes.Keywords: GtfC, GtfD, cariogenic, Streptococcus mutans glucosyltransferase.

scholarly journals Genotypic and Phenotypic Characterization of Streptococcus Mutans Isolated From Dental Caries

2020 ◽  
Author(s):  
Md. Hossain ◽  
Sadab Alam ◽  
Yead Nibir ◽  
Tahrima Tusty ◽  
Sayyeed Bulbul ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Acid Production ◽  
Phenotypic Diversity ◽  
Clinical Isolates ◽  
Specific Gene ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Background: Streptococcus mutans, considered as principal causative agent of dental caries, maintains a biofilm lifestyle in the dental plaque. The oral cavity harbors numerous S. mutans strains which displayed remarkable genotypic and phenotypic diversity. This study evaluated the genotypic and phenotypic diversity of S. mutans strains isolated from patients with dental caries and compared with the universal reference strain UA159.Methods: Selective cultivation on mitis-salivaries-bacitracin agar and species-specific polymerase chain reaction (PCR) was carried out to isolate and identify the 209 S. mutans isolates from 336 patients with dental caries. Arbitrarily primed polymerase chain reaction (AP-PCR), PCR amplification of specific gene, acid production, biofilm formation capacity, and deferred antagonism bacteriocin assay were performed to evaluate the genotypic and phenotypic variation. All statistical analysis was performed using the SPSS software version 20 (SPSS, Chicago, IL, USA).Results: Our study revealed a high degree of genotypic and phenotypic variability among the clinical strains. We observed significant differences in colony morphology, generation time, biofilm formation, bacteriocin and acid production while growing in culture medium. All the clinical isolates were able to lower pH while growing in Todd-Hewitt broth. Consistent with phenotypic variations, we also observed genotypic variation by AP-PCR and gene specific PCR. AP-PCR analysis suggested that most of the patients with dental caries have distinct type of S. mutans strains. Genes related to various two component systems were highly conserved among the isolated strains, however, bacteriocin encoding genes such as nlmAB, nlmC were absent in nearly half of the clinical isolates.Conclusions: Our study highlights the genotypic and phenotypic diversity of S. mutans clinical isolates and indicates the presence of diverse mechanism to initiate and establish the biofilm lifestyle which leads to tooth decay.

scholarly journals Horizontal transmission of Streptococcus mutans in children attending kindergarten

2018 ◽  
Vol 51 (4) ◽  
pp. 216
Author(s):  
Retno Indrawati
Keyword(s):  
Polymerase Chain Reaction ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Horizontal Transmission ◽  
Primary Data ◽  
Inclusion Criteria ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
History Of ◽  
Polymerase Chain

Background: Transmission of Streptococcus mutans (S. mutans), the main pathogen found in dental caries, is particularly prevalent during the first two years of infancy. Numerous children are reportedly infected with S. mutans by their mothers with early initial acquisition of the condition considered to carry a higher risk of subsequent dental caries. Purpose: This research aimed to examine the possibility of horizontal transmission of S. mutans in children attending Surabaya-based kindergartens. Methods: The number of subjects who satisfied the inclusion criteria totaled 146. Dental plaque was collected for one minute with a sterile toothbrush. After completion of an isolation process, 25 S. mutans colonies were identified in Tripticase Cysteine Yeast (TYC) media by means of morphological, microscopic and biochemical tests using API 20 Strept (bioMerieux France). A polymerase chain reaction with OPA-2 and 13 was subsequently used to determine the genotype of S. mutans. Primary data collection was completed by the administering of a questionnaire intended to elicit information regarding the gender, age, diet and medical history of subjects. Results: An arbitrarily primed polymerase chain reaction (AP-PCR) fingerprint profile of the strains isolated from the subjects indicated similarities in five genotypes of S. mutans and differences in 18 genotypes. Conclusion: The study indicated that certain children are similarly infected by S mutans bacteria which might be due to horizontal transmission between classmates.

scholarly journals Detection of streptococcus mutans and streptococcus sobrinus in dental plaque samples from Brazilian preschool children by polymerase chain reaction

2007 ◽  
Vol 18 (4) ◽  
pp. 329-333 ◽  
Author(s):  
Teresa Cristina Costa Franco e Franco ◽  
Patrícia Amoroso ◽  
José Moacir Marin ◽  
Fernando Antonio de Ávila
Keyword(s):  
Polymerase Chain Reaction ◽  
Preschool Children ◽  
Dental Plaque ◽  
Bacterial Species ◽  
Mutans Streptococci ◽  
World Health ◽  
Streptococcus Sobrinus ◽  
Chain Reaction ◽  
Discriminative Ability ◽  
Polymerase Chain

The purposes of this study were to detect S. mutans and S. sobrinus by polymerase chain reaction (PCR) amplification, and to relate their presence to the incidence of dental caries in 42 Brazilian preschool children. Dental plaque samples were collected from the cervical margin of all erupted teeth of 5-6 years old children with primary dentition, using a sterile explorer. Examination of the dmft (decayed, missing, filled teeth) index, performed following the World Health Organization (WHO) caries diagnostic criteria, showed a 2.71 score. Prevalence of S. mutans and S. sobrinus was respectively, of 85.7% and 14.3%; no dental plaque sample was either positive or negative for both bacterial species. Children harboring either S. mutans or S. sobrinus presented the same caries prevalence. PCR showed good discriminative ability for differentiation between these species, and suggested that it is a technique suitable for epidemiological studies on mutans streptococci.

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