Research of actual information on well casing using machine learning and neural networks

In domestic and world practice, despite the measures applied and developed to improve the quality of well casing, there is a problem of leaky structures in almost 50 % of completed wells. The study of actual data using classical methods of statistical analysis (regression and variance analyses) doesn't allow us to model the process with sufficient accuracy that requires the development of a new approach to the study of the attachment process. It is proposed to use the methods of machine learning and neural network modeling to identify the most important parameters and their synergistic impact on the target variables that affect the quality of well casing. The formulas necessary for translating the numerical values of the results of acoustic and gamma-gamma cementometry into categorical variables to improve the quality of probabilistic models are determined. A database consisting of 93 parameters for 934 wells of fields located in Western Siberia has been formed. The analysis of fastening of production columns of horizontal wells of four stratigraphic arches is carried out, the most weighty variables and regularities of their influence on target indicators are established. Recommendations are formulated to improve the quality of well casing by correcting the effects of acoustic and gamma-gamma logging on the results.

A Bridge between Psychoanalysis and Neuroscience: An Overview of the Neurobiological Effects of Psychoanalytical Psychotherapy

This study sets out to investigate the mechanisms by which psychoanalytical psychotherapy can induce neurobiological changes. From Neuroscience which, in accordance with his thinking at the time, Freud never disregarded, the concepts of neuronal plasticity, enriched environment and the neurobiological aspects of the attachment process. From Psychoanalysis, the theory of transference, M. Mahler’s psychological evolution model, the concept of the regulating function of the self-objects and Winnicott’s holding environment concept. Together these provide a useful bridge toward the understanding of the neurobiological changes resulting from psychoanalytical psychotherapy. One concludes that psychoanalytical psychotherapy, through transference, acts as a new model of object relation and learning which furthers the development of certain brain areas, specifically, the right hemisphere, and the prefrontal and limbic cortices, which have a regulating function on affects.

Identifikasi gen kariogenik glukosiltransferase Streptococcus mutans pada pasien karies gigiIdentification of Streptococcus mutans cariogenic gene glucosyltransferase (gtf) in dental caries patients

Pendahuluan: Streptococcus mutans (S. mutans) merupakan bakteri yang mempunyai glukosiltransferase surface (GTFs) yang berperan pada proses perlekatan dan virulensi bakteri pada permukaan gigi. Enzim GTFs terdiri atas tiga jenis enzim GTFs yaitu GTFB, GTFC dan GTFD dan masing-masing enzim ini dikode oleh gen gtfB, gtfC dan gtfD yang juga berperan sebagai prekursor dalam adhesi seluler plak gigi yang dapat menyebabkan karies gigi. Tujuan penelitian adalah mengidentifikasi gen gtfC dan gtfD S. mutans pada pasien karies gigi. Metode: Penelitian dilakukan dengan consecutive sampling. Sampel penelitian diambil dari kerokan jaringan karies gigi, diisolasi, dikultur, dan diidentifikasi di laboratorium Mikrobiologi dan Sentral FK UNRI. Identifikasi S. mutans dan gen kariogenik gtfC dan gtfD menggunakan metode Polymerase Chain Reaction (PCR). Data ditampilkan dalam bentuk tabel distribusi frekuensi dan dinyatakan dalam persentase Hasil: Dari 60 kerokan jaringan karies didapatkan S. mutans sebanyak 10 isolat, sedangkan gen gtfC dan gen gtfD didapatkan masing-masing 4 (40%) isolat. Simpulan: Gen kariogenik teridentifikasi gen gtfC dan gtfD pada isolat S. mutans dari pasien karies gigi sebanyak 40%.Kata kunci: gtfC, gtfD, kariogenik glukosiltransferase Streptococcus mutans. ABSTRACTIntroduction: Streptococcus mutans (S. mutans) is a bacterium with a glucosyltransferase (Gtfs) surface, which plays a role in the attachment process and bacteria virulence on the tooth surface. The Gtfs enzyme consists of three types of Gtfs enzymes, namely GtfB, GtfC, and GtfD. These enzymes are encoded by the GtfB, GtfC, and GtfD genes, which also act as precursors in the cellular adhesion of dental plaque that can cause dental caries. This study was aimed to identify the GtfC and GtfD of S. mutans in dental caries patients. Methods: This study was conducted with consecutive sampling. Research samples were taken from dental caries tissue scrapings, isolated, cultured, and identified in the Microbiology and Central Laboratory of the Faculty of Medicine University of Riau. Identification of S. mutans and GtfC and GtfD cariogenic genes using the Polymerase Chain Reaction (PCR) method. The data was displayed in the form of a frequency distribution table and expressed as a percentage. Results: From 60 caries tissue scrapings, ten isolates were obtained, while the GtfC gene and GtfD gene were obtained 4 (40%) isolates each. Conclusion: Forty percents of S. mutans isolates from dental caries patients were identified as the GtfC and GtfD cariogenic genes.Keywords: GtfC, GtfD, cariogenic, Streptococcus mutans glucosyltransferase.

Shake It Off: The Elimination of Erroneous Kinetochore-Microtubule Attachments and Chromosome Oscillation

Cell proliferation and sexual reproduction require the faithful segregation of chromosomes. Chromosome segregation is driven by the interaction of chromosomes with the spindle, and the attachment of chromosomes to the proper spindle poles is essential. Initial attachments are frequently erroneous due to the random nature of the attachment process; however, erroneous attachments are selectively eliminated. Proper attachment generates greater tension at the kinetochore than erroneous attachments, and it is thought that attachment selection is dependent on this tension. However, studies of meiotic chromosome segregation suggest that attachment elimination cannot be solely attributed to tension, and the precise mechanism of selective elimination of erroneous attachments remains unclear. During attachment elimination, chromosomes oscillate between the spindle poles. A recent study on meiotic chromosome segregation in fission yeast has suggested that attachment elimination is coupled to chromosome oscillation. In this review, the possible contribution of chromosome oscillation in the elimination of erroneous attachment is discussed in light of the recent finding.

Treating Opioid Use Disorder in Peripartum Mothers: A Look at the Psychodynamics, Neurobiology, and Potential Role of Oxytocin

The peripartum period (pregnancy and postpartum) is a unique time filled with psychodynamic and biological changes that are critical in affecting the lives of mother and baby. Attachment, the biologically based emotional connection between a caregiver and infant, is critical to the development of the child. The early interactions in an infant's life shape their reward neuro-circuitry and the development of their internal working models and styles of attachment. Opioid use disorders in the mother affect the psychodynamics and neurobiology of attachment. There is significant overlap between the neurobiology of attachment and that of opioid use disorders. In this article, we hope to describe how opioid use disorders affect mother-infant attachment and how psychodynamic psychotherapy that is informed by attachment theory may be a potential treatment for mothers with opioid use disorders. Further, oxytocin plays a role in the attachment process and may function abnormally in mothers with opioid use disorders. As oxytocin affects attachment, administration of oxytocin during postpartum mother-infant interactions in the setting of psychotherapy may facilitate bonding and promote recovery from opioid use disorders in the peripartum population.

Fault Detection and Classification for Slider Attachment Process using Convolution Neural Network

Hard Disk Drive (HDD) utilizes automation machines for the assembly processes used in the industry to achieve higher production rates and lower costs. The Head Gimbal Assembly (HGA) production process has two main parts: glue dispensing and slider attaching by an Auto Core Adhesion mounting Machine (ACAM). The slider attaching process produces a mounted head to the suspension utilizing vacuum pressure to hold and position a slider. The errors from a vacuum leak from any step trigger system alarms resulting in machine downtime and slider loss defective (SLD). This paper proposes a classification algorithm derived from 250x250 micron images of mounted heads are 4 different categories: Good, Fault I, Fault II and Fault III using Convolution Neural Networks (CNN). CNN is a performance model for predictive maintenance before failure. The method has achieved a 95 % accuracy for detection and classification

The attachment process and physiological properties of Escherichia coli O157:H7 on quartz

Background Manure application and sewage irrigation release many intestinal pathogens into the soil. After being introduced into the soil matrix, pathogens are commonly found to attach to soil minerals. Although the survival of mineral-associated Escherichia coli O157:H7 has been studied, a comprehensive understanding of the attachment process and physiological properties after attachment is still lacking. Results In this study, planktonic and attached Escherichia coli O157:H7 cells on quartz were investigated using RNA sequencing (RNA-seq) and the isobaric tagging for relative and absolute quantitation (iTRAQ) proteomic method. Based on the transcriptomic and proteomic analyses and gene knockouts, functional two-component system pathways were required for efficient attachment; chemotaxis and the Rcs system were identified to play determinant roles in E. coli O157:H7 attachment on quartz. After attachment, the pyruvate catabolic pathway shifted from the tricarboxylic acid (TCA) cycle toward the fermentative route. The survival rate of attached E. coli O157:H7 increased more than 10-fold under penicillin and vancomycin stress and doubled under alkaline pH and ferric iron stress. Conclusions These results contribute to the understanding of the roles of chemotaxis and the Rcs system in the attachment process of pathogens and indicate that the attachment of pathogens to minerals significantly elevates their resistance to antibiotics and environmental stress, which may pose a potential threat to public health.