Molecular Identification and Diveristy of Endophytic Fungi Isolated from Pinus densiflora in Boeun, Korea

Dendrobium are tropical orchid plants that host diverse endophytic fungi. The role of these fungi is not currently well understood in Dendrobium plants. We morphologically and molecularly identified these fungal endophytes, and created an efficient system for evaluating the pathogenicity and symptoms of endophytic fungi on Dendrobium nobile and Dendrobium officinale though in vitro co-culturing. ReThe colony morphological traits of Dendrobium myco-endophytes (DMEs) were recorded for their identification. Molecular identification revealed the presence of Colletotrichum tropicicola, Fusarium keratoplasticum, Fusarium oxysporum, Fusarium solani, and Trichoderma longibrachiatum. The pathogenicity results revealed that T. longibrachiatum produced the least pathogenic effects against D. nobile protocorms. In seedlings, T. longibrachiatum showed the least pathogenic effects against D. officinale seedlings after seven days. C. tropicicola produced highly pathogenic effects against both Dendrobium seedlings. The results of histological examination of infected tissues revealed that F. keratoplasticum and T. longibrachiatum fulfill Koch’s postulates for the existence of endophytes inside the living tissues. The DMEs are cross-transmitted inside the host plant cells, playing an important role in plant host development, resistance, and alkaloids stimulation.

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Molecular identification of endophytic fungi from medicinal plant Huperzia serrata based on rDNA ITS analysis

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-010-0480-x ◽

2010 ◽

Vol 27 (3) ◽

pp. 495-503 ◽

Cited By ~ 20

Author(s):

Xu Yu Chen ◽

Yao Dong Qi ◽

Jian He Wei ◽

Zheng Zhang ◽

De Li Wang ◽

...

Keyword(s):

Medicinal Plant ◽

Molecular Identification ◽

Endophytic Fungi ◽

Huperzia Serrata ◽

Its Analysis ◽

Rdna Its

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Endophytic fungi associated with healthy pine needles and needles infested by the pine needle gall midge, Thecodiplosis japonensis

Canadian Journal of Botany ◽

10.1139/b95-040 ◽

1995 ◽

Vol 73 (3) ◽

pp. 384-390 ◽

Cited By ~ 66

Author(s):

Kunihiko Hata ◽

Kazuyoshi Futai

Keyword(s):

Endophytic Fungi ◽

Pinus Densiflora ◽

Gall Midge ◽

Pine Needles ◽

Basal Region ◽

Ecological Groups ◽

Gall Formation ◽

Pine Needle ◽

Hybrid Pine ◽

Pine Needle Gall Midge

The endophytic mycobiota of the galls of pine needle gall midge, Thecodiplosis japonensis, and healthy current-year needles of Pinus densiflora and the F2 hybrid pine (Pinus thunbergii × (P. thunbergii × P. densiflora)) was compared. Phialocephala sp. was the dominant endophyte isolated from the galls and from the basal regions of healthy needles. Species richness in endophytes was facilitated in galls compared to the basal region of healthy needles, and the commencement of their colonization was hastened in galls. In the early stages of gall formation, however, no endophyte was isolated, suggesting that gall endophytes are not carried into the galls by the midge. Endophytes of healthy and galled pine needles were divided into two groups: position-specific fungi, which showed intrinsically restricted distributions on needles; and gall-specific fungi. These two groups might represent different ecological groups of endophytes. Possible interactions between endophytes and the pine needle gall midge are also discussed. Key words: endophytic fungi, Pinus densiflora, F2 hybrid pine, pine needle gall midge.

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Isolation, antibacterial activity, and molecular identification of endophytic fungi from Pogostemon cablin

Journal of Physics Conference Series ◽

10.1088/1742-6596/1943/1/012066 ◽

2021 ◽

Vol 1943 (1) ◽

pp. 012066

Author(s):

N A Pratama ◽

M M Widyarifa ◽

S L Farikha ◽

H P Kusumaningrum ◽

Y Eshananda

Keyword(s):

Antibacterial Activity ◽

Molecular Identification ◽

Endophytic Fungi ◽

Pogostemon Cablin

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Molecular Identification of Endophytic Fungi Isolated from Needle Leaves of Pinus thunbergii

The Korean Journal of Mycology ◽

10.4489/kjm.2012.40.4.183 ◽

2012 ◽

Vol 40 (4) ◽

pp. 183-186 ◽

Cited By ~ 1

Author(s):

Chang-Kyun Kim ◽

Ju-Kyeong Eo ◽

Ahn-Heum Eom

Keyword(s):

Molecular Identification ◽

Endophytic Fungi ◽

Pinus Thunbergii

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Identification and Antibacterial Characterization of Endophytic Fungi from Artemisia sieberi

International Journal of Microbiology ◽

10.1155/2021/6651020 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Ibtisam Mohammed Ababutain ◽

Sahar Khamees Aldosary ◽

Amal Abdulaziz Aljuraifani ◽

Azzah Ibrahim Alghamdi ◽

Amira Hassan Alabdalall ◽

...

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Molecular Identification ◽

Endophytic Fungi ◽

Pathogenic Bacteria ◽

Antibacterial Properties ◽

Diffusion Method ◽

Rrna Gene ◽

Gram Positive ◽

Artemisia Sieberi

Endophytic fungi serve as a reservoir for important secondary metabolites. The current study focused on the antibacterial properties of endophytic fungi isolated from Artemisia sieberi. Initially, six endophytic fungi were isolated and purified from the stem of A. sieberi. Endophytic fungi were identified by morphological characteristics, as well as by molecular identification using 18S rRNA gene sequencing method. All the six isolates were subjected to the preliminary screening for their antibacterial activity against nine important pathogenic bacteria using the disk-diffusion method. Crude extracts of the most active isolate were obtained using ethyl acetate. Antibacterial activity of the ethyl acetate extract was evaluated using well diffusion method on the selected isolate. The antibacterial efficiency of the selected isolate was evaluated by determining the Minimum Inhibitory Concentration (MIC). MIC values were in appreciable quantity against both Gram-positive and Gram-negative bacteria ranging from 3.125 to 6.25 µg/mL and 12.5 to 50 µg/mL, respectively. This result indicated that Gram-positive bacteria were more susceptible to the endophytic fungi extract. Moreover, the molecular identification results revealed that all the isolates belong to Ascomycota and represented Aspergillus and Penicillium genera and three species: A. oryzae (three isolates), A. niger (one isolate), and P. chrysogenum (two isolates). All six endophytic fungi were able to inhibit the growth of at least two of the tested bacteria. Among the isolated strains, isolate AS2, which identified as P. chrysogenum, exhibited the highest antibacterial activity against all nine tested bacteria and was higher than or equal to the positive control against most of the tested bacteria. Future studies are required to isolate and identify these bioactive substances, which can be considered as a potential source for the synthesis of new antibacterial drugs to treat infectious diseases.

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