Molecular Identification of Endophytic Fungi from Banana Leaves (Musa spp.)

Dendrobium are tropical orchid plants that host diverse endophytic fungi. The role of these fungi is not currently well understood in Dendrobium plants. We morphologically and molecularly identified these fungal endophytes, and created an efficient system for evaluating the pathogenicity and symptoms of endophytic fungi on Dendrobium nobile and Dendrobium officinale though in vitro co-culturing. ReThe colony morphological traits of Dendrobium myco-endophytes (DMEs) were recorded for their identification. Molecular identification revealed the presence of Colletotrichum tropicicola, Fusarium keratoplasticum, Fusarium oxysporum, Fusarium solani, and Trichoderma longibrachiatum. The pathogenicity results revealed that T. longibrachiatum produced the least pathogenic effects against D. nobile protocorms. In seedlings, T. longibrachiatum showed the least pathogenic effects against D. officinale seedlings after seven days. C. tropicicola produced highly pathogenic effects against both Dendrobium seedlings. The results of histological examination of infected tissues revealed that F. keratoplasticum and T. longibrachiatum fulfill Koch’s postulates for the existence of endophytes inside the living tissues. The DMEs are cross-transmitted inside the host plant cells, playing an important role in plant host development, resistance, and alkaloids stimulation.

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Molecular identification of endophytic fungi from medicinal plant Huperzia serrata based on rDNA ITS analysis

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-010-0480-x ◽

2010 ◽

Vol 27 (3) ◽

pp. 495-503 ◽

Cited By ~ 20

Author(s):

Xu Yu Chen ◽

Yao Dong Qi ◽

Jian He Wei ◽

Zheng Zhang ◽

De Li Wang ◽

...

Keyword(s):

Medicinal Plant ◽

Molecular Identification ◽

Endophytic Fungi ◽

Huperzia Serrata ◽

Its Analysis ◽

Rdna Its

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Isolation, antibacterial activity, and molecular identification of endophytic fungi from Pogostemon cablin

Journal of Physics Conference Series ◽

10.1088/1742-6596/1943/1/012066 ◽

2021 ◽

Vol 1943 (1) ◽

pp. 012066

Author(s):

N A Pratama ◽

M M Widyarifa ◽

S L Farikha ◽

H P Kusumaningrum ◽

Y Eshananda

Keyword(s):

Antibacterial Activity ◽

Molecular Identification ◽

Endophytic Fungi ◽

Pogostemon Cablin

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Molecular Identification of Endophytic Fungi Isolated from Needle Leaves of Pinus thunbergii

The Korean Journal of Mycology ◽

10.4489/kjm.2012.40.4.183 ◽

2012 ◽

Vol 40 (4) ◽

pp. 183-186 ◽

Cited By ~ 1

Author(s):

Chang-Kyun Kim ◽

Ju-Kyeong Eo ◽

Ahn-Heum Eom

Keyword(s):

Molecular Identification ◽

Endophytic Fungi ◽

Pinus Thunbergii

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Identification and Antibacterial Characterization of Endophytic Fungi from Artemisia sieberi

International Journal of Microbiology ◽

10.1155/2021/6651020 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Ibtisam Mohammed Ababutain ◽

Sahar Khamees Aldosary ◽

Amal Abdulaziz Aljuraifani ◽

Azzah Ibrahim Alghamdi ◽

Amira Hassan Alabdalall ◽

...

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Molecular Identification ◽

Endophytic Fungi ◽

Pathogenic Bacteria ◽

Antibacterial Properties ◽

Diffusion Method ◽

Rrna Gene ◽

Gram Positive ◽

Artemisia Sieberi

Endophytic fungi serve as a reservoir for important secondary metabolites. The current study focused on the antibacterial properties of endophytic fungi isolated from Artemisia sieberi. Initially, six endophytic fungi were isolated and purified from the stem of A. sieberi. Endophytic fungi were identified by morphological characteristics, as well as by molecular identification using 18S rRNA gene sequencing method. All the six isolates were subjected to the preliminary screening for their antibacterial activity against nine important pathogenic bacteria using the disk-diffusion method. Crude extracts of the most active isolate were obtained using ethyl acetate. Antibacterial activity of the ethyl acetate extract was evaluated using well diffusion method on the selected isolate. The antibacterial efficiency of the selected isolate was evaluated by determining the Minimum Inhibitory Concentration (MIC). MIC values were in appreciable quantity against both Gram-positive and Gram-negative bacteria ranging from 3.125 to 6.25 µg/mL and 12.5 to 50 µg/mL, respectively. This result indicated that Gram-positive bacteria were more susceptible to the endophytic fungi extract. Moreover, the molecular identification results revealed that all the isolates belong to Ascomycota and represented Aspergillus and Penicillium genera and three species: A. oryzae (three isolates), A. niger (one isolate), and P. chrysogenum (two isolates). All six endophytic fungi were able to inhibit the growth of at least two of the tested bacteria. Among the isolated strains, isolate AS2, which identified as P. chrysogenum, exhibited the highest antibacterial activity against all nine tested bacteria and was higher than or equal to the positive control against most of the tested bacteria. Future studies are required to isolate and identify these bioactive substances, which can be considered as a potential source for the synthesis of new antibacterial drugs to treat infectious diseases.

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Molecular identification of endophytic fungi from torch ginger (Etlingera elatior) antagonist to phytopathogenic fungi

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210641 ◽

2020 ◽

Vol 21 (6) ◽

Author(s):

Anisa Lutfia ◽

Erman Munir ◽

Yurnaliza Yurnaliza

Keyword(s):

Molecular Identification ◽

Endophytic Fungi ◽

Phytopathogenic Fungi ◽

Dual Culture ◽

Trichoderma Spp ◽

Rdna Its ◽

Rigidoporus Lignosus ◽

Plant Families ◽

Relationship Of ◽

Etlingera Elatior

Abstract. Lutfia A, Munir E, Yurnaliza. 2020. Molecular identification of endophytic fungi from torch ginger (Etlingera elatior) antagonist to phytopathogenic fungi. Biodiversitas 21: 2681-2689. Medicinal plants are valuable sources in exploration of endophytic microorganisms. The utilization of culturable endophytes, particularly endophytic fungi in pharmacological aspects has been reported in many literature. Zingiberaceae, being known as one of medicinal plant families, is commonly used in tropical countries, e.g Indonesia to treat health disorders and ailments. However, little information has been reported upon their associative endophytic fungi that may be used as biocontrol agents against phytopathogenic fungi. Efforts to elaborate on the symbiotic relationship between endophytic fungi and host to explain the antifungal ability from endophytes are presented in this study. Six fungal morphotypes were recovered from the rhizome fragment of torch ginger (Etlingera elatior (Jack) RM Smith) sampled from Sibayak forest, North Sumatra, Indonesia. All isolates displayed potential antifungal activities against Fusarium oxysporum, Ganoderma boninense, and Rigidoporus lignosus as representative phytopathogens in a dual culture plate assay. All fungal morphotypes were identified based on their 18S rDNA-ITS regions. Molecular identification revealed that most isolates were related to Trichoderma spp. while one isolate was identified as Pestaliotopsis sp. The endophytic assemblage of mostly Trichoderma strains in our study may reveal a mutual relationship of these soil fungi as root colonizer displaying biocontrol properties against phytopathogenic fungi to host.

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