scholarly journals Penyebaran Kutikula Pada Kerabang Telur Pada Usaha Peternakan Ayam Dengan Sistem Free Range

2022 ◽  
Vol 11 (3) ◽  
pp. 257
Author(s):  
Elfira Kariane Suawa ◽  
J R Roberts
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Egg Quality ◽  
Production Cycle ◽  
Blue Dye ◽  
Mean Values ◽  
Free Range ◽  
Single Score ◽  
Level Of Significance ◽  
Scanning Electron

Abstract The cuticle is the outermost layer of the egg which deposited on to the surface of the egg during the final 1-1.5 hours prior to oviposition. It is a protective coating which prevents bacterial penetration through the gas exchange pores in the eggshell. The aim of this research is analysing the cuticle cover and ultrastructural features of the mammillary layer cover during production cycle in the free-range system. Data were collected from age 26, 37, 50 and 60 weeks of age to observe the eggshell colour (%), cuticle cover following the MST blue dye which analysed by spectrophotometry based on L*a*b system and scanning electron microscopy (SEM). Data were analysed using Statview Software (SAS Institute Inc., Version 5.0.1.0). A two way analysis of variance was conducted taking flock age and shed/flock as the independent variables and body weight, egg quality measurements, spectrophotometry (L*a*b) measurements, single score measurements for cuticle cover, and ultrastructural features as dependent variables. Level of significance was indicated by probability of less than 5%. The Fishers PLSD test was used to differentiate between mean values. The result of this research showed that the cuticle cover is vary from age to age. The greater value of cuticle cover in at 37 weeks of age which the same result also showed by single scrore and SEM. The cuticle cover of the shell is mostly affected by age and also strain. Keyword: Cuticle; Eggshell quality; Laying hens.   Abstrak Kutikula pada telur merupakan lapisan terluar telur yang dideposisikan pada lapisan palisade kurang lebih 1,5 – 2 jam di akhir pembentukan kerabang di dalam uterus. Kutikula berfungsi sebagai benteng pertahanan pertama untuk mencegah masuknya bakteri ke dalam telur. Penelitian ini dilakukan pada usaha peternakan ayam petelur yang dipelihara dengan system free range. Telur diambil untuk dianalisa penyebaran kutikula telur pada umur 26, 37, 50, dan 60 minggu. Variable yang diamati meliputi reflektifitas warna kerabang (%), penyebaran kutikula diukur menggunakan MST blue dye dan selanjutnya diukur melalui sistim spectrophotometry, yang difokuskan pada sistim pewarnaan berdasarkan sistim L*a*b* dan single score, dan dilanjutkan dengan pengamatan menggunakan scanning electron microscopy (SEM).Selanjutnya data dianalisis menggunakan Staview software (SAS Institute Inc. version 5.0.1.0) dengan analisis anova dua arah, dimana umur ayam sebagai variable independent dan nilai SCI a* sesudah perendaman dan nilai single score (ΔE*ab) sebagai variable dependent, pada level of significance 5%. Pengaruh yang nyata diuji lanjut menggunakan Fisher PLSD. Pada penelitian ini didapatkan bahwa penyebaran kutikula pada kerabang telur bervariasi selama pengamatan. Penyebaran kutikula terbanyak ditemukan pada saat ayam berumur 37 minggu. Hal ini juga diperkuat dengan hasil yang diperoleh dengan single score (ΔE*ab) yang memperlihatkan nilai ΔE*ab yang tinggi pada umur 37 minggu. Penyebaran kutikula pada kerabang telur sangat dipengaruhi oleh umur dan juga strain ayam. Kata kunci: Ayam petelur;  Kualitas telur; Kutikula.

scholarly journals Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy

2019 ◽  
Vol 13 ◽  
pp. 1
Author(s):  
Priscila Bezerra Dos Santos Melo ◽  
Ana Cristina Portugal Pinto de Carvalho ◽  
Cândida Hermínia Campos de Magalhães Bertini ◽  
Celli Rodrigues Muniz ◽  
Adroaldo Guimarães Rossetti
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Somatic Embryogenesis ◽  
Callus Formation ◽  
Nodal Segments ◽  
Embryogenic Calli ◽  
Evaluation Period ◽  
Mean Values ◽  
Embryogenic Callus Formation ◽  
Scanning Electron

Somatic embryogenesis is an advantageous tool in the commercial production of micropropagated anthurium plantlets. As such, the aim of this study was to establish a protocol for the induction of somatic embryogenesis in Jureia and Luau cultivars. Defoliated nodal segments, 1.0 cm in length and containing one bud, were used as explants. The experimental design was completely randomised, in a 2 x 3 x 5 factorial scheme (cultivar: Jureia and Luau x auxin: 2,4-D, NAA and Picloram x concentration: 0, 2.5, 5.0, 7.5, and 10.0 μM), with 30 treatments in a scheme of plots split over time (15, 30, 45, 60, 75 and 90 days). The anatomy and percentage of embryogenic callus formation were analysed. The structures formed, analysed by scanning electron microscopy, corresponded to embryogenic calli. The Luau cultivar was superior in forming embryogenic calli. For the two cultivars, among the auxins under study, NAA demonstrated a greater induction potential for somatic embryogenesis, with the concentration of 7.5 μM giving the highest mean values. The 90-day evaluation period showed the maximum formation of embryogenic calli; however, mean values were fairly similar to the 75-day evaluation period. To induce embryogenic calli, therefore, it is suggested that the nodal segments be inoculated into a culture medium with added NAA growth regulator at a concentration of 7.5 μM, and that the explants remain in this medium for 75 days after inoculation.

Extra-alveolar veins are contiguous with, and leak fluid into, periarterial cuffs in rabbit lungs

1991 ◽  
Vol 71 (4) ◽  
pp. 1606-1613 ◽  
Author(s):  
D. L. Luchtel ◽  
L. Embree ◽  
R. Guest ◽  
R. K. Albert
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Interstitial Fluid ◽  
Blue Dye ◽  
Alveolar Pressure ◽  
Vessel Lumen ◽  
Freeze Dried ◽  
Orange G ◽  
Pulmonary Arterial ◽  
Scanning Electron

We previously observed physiological evidence that arterial and venous extra-alveolar vessels shared a common interstitial space. The purpose of the present investigation was to determine the site of this continuity to improve our understanding of interstitial fluid movement in the lung. Orange G and Evans blue dyes were added to the arterial and venous reservoirs, respectively, of excised rabbit lungs as they were placed 20 cmH2O into zone 1 (pulmonary arterial and venous pressures = 5 cmH2O, alveolar pressure = 25 cmH2O). After 10 s or 4 h the lungs were fixed by immersion in liquid N2, freeze-dried, cut into 5-mm serial slices, and examined by light macroscopy. Serial sections of 0.25–0.5 mm were subsequently examined by scanning electron microscopy. In the animals subjected to the zone 1 stress for 4 h, arterial and venous extra-alveolar vessels were surrounded by cuffs of edema. The edema ratio (cuff area divided by vessel lumen area) was greater around arteries than veins and decreased with increasing vessel size. Periarterial cuffs usually contained orange dye and frequently contained both orange and blue dye. Lymphatics containing orange or blue dye were frequently seen in periarterial cuffs. Scanning electron microscopy demonstrated that extra-alveolar veins of approximately 100 microns diameter were anatomically contiguous with arterial extra-alveolar vessel cuffs. In rabbit lungs, both arterial and venous extra-alveolar vessels (and/or alveolar corner vessels) leak fluid into perivascular cuffs surrounding arterial extra-alveolar vessels, and lymphatics located in the periarterial cuff contain fluid that originates from both the arterial and venous extra-alveolar vessels.

scholarly journals Scanning electron microscopy analysis of dental cements

2009 ◽  
Vol 63 (4) ◽  
pp. 281-288
Author(s):  
Radivoje Radosavljevic ◽  
Sasa Stankovic ◽  
Zorica Ajdukovic ◽  
Danimir Jevremovic ◽  
Jelena Todic
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sem Analysis ◽  
Luting Cements ◽  
Mean Values ◽  
Luting Agents ◽  
Maximal Diameter ◽  
Zinc Phosphate Glass ◽  
Scanning Electron

The aim of this study was to compare in vitro the characteristics of different types of luting cements (zinc phosphate, glass-ionomer and resin based composite cement) using scanning electron microscopy (SEM) analysis and microleakage for the quality range of materials. Dental cements were mixed in accordance with the manufacturer's instructions and formed with posts in dental root canals of extracted teeth. The quality of cement was determined by SEM observation on horizontal sectioned roots with fixed posts according to specific pore and marginal gap diameter. The microleakage was measured on specimens immersed in Lofler (methylene blue) solution. The mean values of the maximal diameter of pores, marginal gaps and microleakage of conventional cements are remarkably larger in comparison with composite luting agents. In conclusion, the quality and efficiency of composite luting agents in comparison with conventional cements are more successful in protecting the interior of tooth from penetration of oral fluids, bacteria and bacterial toxins into unprotected dentine.

BSS Plus: a potential irrigating solution for neurosurgery

1986 ◽  
Vol 64 (6) ◽  
pp. 911-917
Author(s):  
Glenn A. Meyer ◽  
Dennis J. Maiman ◽  
Henry F. Edelhauser ◽  
O. J. Lorenzetti ◽  
John Garancis
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Normal Saline ◽  
Blue Dye ◽  
Saline Irrigation ◽  
Content Type ◽  
Transmission Electron ◽  
Significant Difference ◽  
Irrigation Solution ◽  
Scanning Electron

✓ BSS Plus is a pH-stable balanced salt solution similar to glutathione bicarbonate Ringer's solution. Extensively used in ophthalmology, it is of potential value in neurosurgery. In comparative tests of its effectiveness, 28 cats underwent bilateral irrigation of the surface of the cerebral cortex with normal saline on one side and BSS Plus on the other. After 2 hours, a marked decrease was seen in the surface pH of the hemisphere irrigated with normal saline but not of the hemisphere treated with BSS Plus. Blood-brain barrier changes (measured with Evans blue dye techniques) were more evident following saline irrigation. Somatosensory evoked potentials and cerebral blood flow were not significantly altered. Conventional light microscopy using three standard stains did not reveal a significant difference. Transmission electron microscopy studies were performed in 14 animals and scanning electron microscopy in six. In five animals both transmission and scanning electron microscopy studies were conducted after irrigation with both agents without a cottonoid cover and with immediate harvest of superficial layers from the living brain and immersion-fixation in glutaraldehyde. Tissue preservation was superior on the BSS Plus side in all studies. This agent may represent an improved irrigation solution for neurosurgery, but further studies are required.

scholarly journals Effects of intracameral brilliant blue on the corneal endothelium of swine: in vitro study

2016 ◽  
Vol 36 (8) ◽  
pp. 775-780 ◽  
Author(s):  
Mariana Terzariol ◽  
Paula S. Hünning ◽  
Gustavo Brambatti ◽  
Luciane de Albuquerque ◽  
Carolina Neumann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Anterior Chamber ◽  
Corneal Endothelium ◽  
Brilliant Blue ◽  
Ultrastructural Changes ◽  
Blue Dye ◽  
Endothelium Cells ◽  
The Right ◽  
Scanning Electron

Abstract: The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue±) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution injection and the cornea was excised too. All the corneas were evaluated by scanning electron microscopy to evaluate the changes on the endothelium caused by the brilliant blue dye. There were no significant differences between the right corneal endothelium cells and the left corneal endothelium cells with scanning electron microscopy after intracameral use of 0.05% brilliant blue dye. The 0.05% brilliant blue dye concentration did not cause deleterious effects for the swine corneal endothelium after intracameral use and can be a choice for safe staining of the anterior capsule of the lens in cataract surgery.

scholarly journals Evaluation of Cervico-occlusal Dimensions of Maxillary and Mandibular Incisor Brackets for Lingual Orthodontics

2014 ◽  
Vol 15 (3) ◽  
pp. 306-311 ◽  
Author(s):  
Andréia Cotrim-Ferreira ◽  
Flávio Cotrim-Ferreira ◽  
Flávio Vellini-Ferreira ◽  
Daniele Fernanda Peron ◽  
Carlos Carvalho ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Reference Points ◽  
Mandibular Incisor ◽  
Mean Values ◽  
Lingual Orthodontics ◽  
Future Studies ◽  
The Mean ◽  
Scanning Electron

ABSTRACT Aim The purpose of this study was to analyze the cervicoocclusal dimensions of brackets used in lingual orthodontics for maxillary and mandibular central and lateral incisors, to compare them with the numbers informed by the manufacturer. Materials and methods The brackets were placed in a template and analyzed by scanning electron microscopy (SEM), evaluating the images with the aid of a software that allowed determination of reference points, tracing of lines on the bracket images and measurement of the distances. Results Brackets manufactured by Dm Ceosa Hiro, Tecnident PSWb and Dentaurum Magic did not present statistically significant differences between the mean values of slot heights and the values informed by manufacturers. Conversely, the brands Ormco 7th generation, Ormco STb, GAC Innovation L, ORJORG, Tecnident, Ormco STb LSW and Adenta evolution STL exhibited statistically significant differences. Conclusion In general, there was a deficiency in the standardization of cervico-occlusal measurements of slots of the brackets analyzed. Clinical significance Manufacturers should be attentive to the quality control of their materials. Future studies will analyze the actual clinical influence of these findings on the orthodontic mechanics. How to cite this article Cotrim-Ferreira A, Cotrim-Ferreira F, Vellini-Ferreira F, Peron DF, Carvalho C, Torres FC. Evaluation of Cervico-occlusal Dimensions of Maxillary and Mandibular Incisor Brackets for Lingual Orthodontics. J Contemp Dent Pract 2014;15(3):306-311.

scholarly journals Optical and photocatalytic properties of TiO2 nanoplumes

2017 ◽  
Vol 8 ◽  
pp. 190-195 ◽  
Author(s):  
Viviana Scuderi ◽  
Massimo Zimbone ◽  
Maria Miritello ◽  
Giuseppe Nicotra ◽  
Giuliana Impellizzeri ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Dye Degradation ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
Photocatalytic Efficiency ◽  
Transmission Electron ◽  
Uv Photodegradation ◽  
Scanning Electron ◽  
Ti Films

Here we report the photocatalytic efficiency of hydrogenated TiO2 nanoplumes studied by measuring dye degradation in water. Nanoplumes were synthesized by peroxide etching of Ti films with different thicknesses. Structural characterization was carried out by scanning electron microscopy and transmission electron microscopy. We investigated in detail the optical properties of the synthesized material and related them to the efficiency of UV photodegradation of methylene blue dye. The obtained results show that TiO2 nanoplumes act as an effective antireflective layer increasing the UV photocatalytic yield of the film.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Sign in / Sign up

Export Citation Format

Share Document