scholarly journals Matrix metalloproteinases in children with uncomplicated compression fractures of the spine

2021 ◽  
Vol 24 (2) ◽  
pp. 96-101
Author(s):  
I. E. Smirnov ◽  
O. V. Karaseva ◽  
A. P. Fisenko ◽  
A. G. Kucherenko ◽  
V. A. Mitish ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Blood Serum ◽  
Reference Group ◽  
Spinal Injury ◽  
Blood Levels ◽  
Tissue Inhibitor ◽  
Compression Fractures ◽  
Fractures Of The Spine ◽  
Reparative Process ◽  
And Control

The aim of the study was to determine changes in the content of matrix metalloproteinases (MMP) and their tissue inhibitor in children with uncomplicated compression fractures of the spine (UCFS). Materials and methods. Eighty-five children, including 69 patients with UCFS (average age 12.3 ± 2.6 years), were comprehensively examined. The reference group consisted of 16 children (average age 11.8 ± 2.7 years) without spinal pathology. During the diagnostic period for 1-3 days, changes in the MMP content and their tissue inhibitor (TIMP-1) in blood serum were determined by the enzyme immunoassay method in all children after trauma. Results. It was found that in the acute period after spinal injury, the blood levels of gelatinases (MMP-2 and MMP-9), stromelysin (MMP-3), and collagenases (MMP-8) significantly increased compared to their levels in children of the reference group. At the same time, the levels of TIMP-1 and the ratio of MMP/TIMP-1 concentrations in the blood of patients with UCFS significantly decreased compared to the control, which indicates the predominance of the proteolytic effect of MMP. Analysis of changes in the content of MMP in the blood in UCFS boys and girls did not reveal significant differences in the levels of the studied MMP and TIMP-1, except for a significant increase in the concentrations of stromelysin (MMP-3) in the blood serum of boys compared with its level in girls and the control. With different severity of the course of UCFS in children, a significant increase in MMP concentrations associated with an increase in the severity of the injury was revealed, and a substantial decrease in the content of TIMP-1 in the blood of patients compared to its levels in children with 1-2 degrees of severity and control. Conclusion. The established patterns indicate that the determination of the content of MMR and TIMP-1 in the blood in UCFS children allows monitoring the course of the reparative process after injury to the vertebral bodies in children.

Prognostic Role of Matrix Metalloproteinases 2, 7, 8, 9 and Their Type 1 Tissue Inhibitor in Blood Serum of Patients with Kidney Cancer

2020 ◽  
Vol 168 (5) ◽  
pp. 673-676
Author(s):  
N. E. Kushlinskii ◽  
E. S. Gershtein ◽  
A. A. Alferov ◽  
S. D. Bezhanova ◽  
V. V. Mushtenko ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Blood Serum ◽  
Kidney Cancer ◽  
Prognostic Role ◽  
Tissue Inhibitor

scholarly journals Matrix metalloproteinase-9, -10, and tissue inhibitor of matrix metalloproteinases-1 blood levels as biomarkers of severity and mortality in sepsis

Critical Care ◽  
2009 ◽  
Vol 13 (5) ◽  
pp. R158 ◽  
Author(s):  
Leonardo Lorente ◽  
María M Martín ◽  
Lorenzo Labarta ◽  
César Díaz ◽  
Jordi Solé-Violán ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 9 ◽  
Blood Levels ◽  
Tissue Inhibitor ◽  
Metalloproteinase 9

scholarly journals BLOOD MATRIX METALLOPROTEINASES LEVELS IN CYSTIC FIBROSIS CHILDREN (TEN YEARS OBSERVATION)

2019 ◽  
Vol 21 (5) ◽  
pp. 279-284
Author(s):  
Maksim S. Egorov
Keyword(s):  
Cystic Fibrosis ◽  
Growth Factor ◽  
Matrix Metalloproteinases ◽  
Blood Serum ◽  
Transforming Growth Factor ◽  
Reference Group ◽  
Transforming Growth Factor Β1 ◽  
Long Time ◽  
Ct Data ◽  
Tgf Β1

Introduction. Destructive fibrotic changes in lung tissue play a key role in the pathogenesis of cystic fibrosis (CF) in children. The development of pulmonary fibrosis may be caused by a violation of the pattern of matrix metalloproteinases (MMPs) and elevated production of profibrogenic growth factors (TGF-β1). Aim of the study. To compare the peculiarities of MMP patterns and transforming growth factor TGF-β1 with the data of the visualisation of airways features in cystic fibrosis (CF) children. Patients and Methods. The study included 80 inpatients aged of from 3 months to 18 years suffered from СF with the involvement of the lungs and digestive system observed for ten years. All patients were administered antibiotics (cefoperazone/sulbactam, ceftazidime, tienam, meropenem, amikacin) and inhalation (colisthmethate sodium, tobramycin) intravenously for a long time period. The reference group consisted of 16 children without pulmonary pathology. Blood serum concentrations of transforming growth factor-β1 (TGF-β1), matrix metalloproteinases (MMP-2, MMP-8, MMP-9) and tissue inhibitor of matrix metalloproteinases (TIMP-1) were determined by ELISA method. The morphological features of airways were evaluated by means of computer tomography (CT) with (GE Discovery CT750 HD). Results. In CF children patients blood serum MMP9 levels were significantly higher whereas TIMP-1 and MMP-2 appeared to be less than in children with intact airways. TGF-β1 levels in CF children were 9.8 times more than in cases from the reference group. CT data showed the pronounced changes in the airways structure as multiple bronchoectasias and pneumofibrosis. Conclusion. The revealed morphologic signs of the deterioration in airways’ structure in СF children patients can be related to the elevation of the rate of the fibrosis development due to the violation in the MMP and profibrogenic factors patterns and transforming growth factor TGF-β1.

scholarly journals MATRIX METALLOPROTEINASES IN CHILDREN WITH CYSTIC FIBROSIS

2019 ◽  
Vol 21 (3) ◽  
pp. 145-151
Author(s):  
I. E. Smirnov ◽  
A. G. Kucherenko ◽  
M. S. Egorov ◽  
G. I. Smirnova ◽  
Tsevegmid Urtnasan ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Matrix Metalloproteinases ◽  
Blood Serum ◽  
Transforming Growth Factor ◽  
Reference Group ◽  
Transforming Growth Factor Β1 ◽  
Healthy Children ◽  
Elisa Method ◽  
Tgf Β1

The data of examination of 80 in-patients with the mixed form of cystic fibrosis (CF) are presented. All cases were divided into 3 groups according to the severity of the course of the disease. 16 conditionally healthy children made up a reference group. Determination of blood serum concentrations of interleukins (IL4, IL6), transforming growth factor-β1 (TGF-β1), matrix metalloproteinases MMP-2, MMP-8, MMP-9 and tissue inhibitor-TIMP-1 was performed by immunoassay ELISA method. The changes in the content of MMP and TIMP-1 in the blood serum of patients with various severity of the course of CF were found to be characterized by a significant decrease in MMP-8 and TIMP-1 concentrations, an increase in MMP-2 levels in children with moderate СF and a significant increase in MMP-9 concentrations, especially pronounced in patients with severe CF. At the same time, no definite dependence of the changes in MMP and TIMP-1 concentrations in the blood serum of patients on the frequency of exacerbations in the CF course and the dominant microbiota was found. Changes in the content of IL and TGF-β1 in the blood serum of children with the various severity of the course of CF were characterized by an increase in the concentrations of IL4 and TGFβ1 by more than 9.8 times, and IL6 - by 4.6 times if compared with the reference group. However, there no direct correlation was found between the changes in their production and the severity of the course of CF. The authors believe elevated levels of MMP, TIMP, and altered relationships between them can be used as biomarkers of the exacerbation of CF course in children.

scholarly journals Detection of a latent soluble form of membrane type 1 matrix metalloprotease bound with tissue inhibitor of matrix metalloproteinases-2 in periprosthetic tissues and fluids from loose arthroplasty endoprostheses

FEBS Journal ◽  
2013 ◽  
Vol 280 (24) ◽  
pp. 6541-6555 ◽  
Author(s):  
Anna Niarakis ◽  
Eleftheria Giannopoulou ◽  
Panagiota Ravazoula ◽  
Elias Panagiotopoulos ◽  
Ioannis K. Zarkadis ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Soluble Form ◽  
Matrix Metalloprotease ◽  
Tissue Inhibitor ◽  
Membrane Type

scholarly journals Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na99mTcO4) in rats

2009 ◽  
Vol 24 (5) ◽  
pp. 383-386 ◽  
Author(s):  
Cecília Maria de Carvalho Xavier Holanda ◽  
Monique Batista da Costa ◽  
Natália Chilinque Zambão da Silva ◽  
Maurício Ferreira da Silva Júnior ◽  
Vanessa Santos de Arruda Barbosa ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Aloe Vera ◽  
Treated Group ◽  
Control Group ◽  
Blood Levels ◽  
Control Groups ◽  
High Increase ◽  
Tropical Plant ◽  
And Control ◽  
The Brain

PURPOSE: Aloe vera is a tropical plant popularly known in Brazil as babosa. We have investigated the effect of aqueous extract of Aloe vera on the biodistribution of Na99mTcO4 and laboratorial parameters in Wistar rats. METHODS: Twelve animals were divided into treated and control groups. In the treated group, Aloe vera was given by gavage (5mg/mL/day) during 10 days. The control group received sorbitol by the same way and period. One hour after the last dose, we injected 0.1mL of Na99mTcO4 by orbital plexus. After 60 min, all the animals were killed. Samples were harvested from the brain, liver, heart, muscle, pancreas, stomach, femur, kidneys, blood, testis and thyroid and the percentage of radioactivity (%ATI/g) was determined. Biochemical dosages were performed. RESULTS: There was a significant increase of %ATI/g in blood, femur, kidneys, liver, stomach, testis and thyroid and also in blood levels of AST and ALT. A significant decrease in levels of glucose, cholesterol, triglycerides, creatinine and urea occurred. The statistical analyses were performed by Mann-Whitney test and T-Student test (p<0.05). CONCLUSION: The aqueous extract of Aloe vera facilitated the uptake of Na99mTcO4 in organs of rats and it was responsible to a high increase of levels of AST and ALT.

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