scholarly journals FTIR Analysis of Biodegradation of Polystyrene by Intestinal Bacteria Isolated from Zophobas Morio and Tenebrio Molitor

2020 ◽  
Author(s):  
Hong-Han Lin ◽  
Hsiao-Han Liu
Keyword(s):  
Culture Medium ◽  
Marine Pollution ◽  
Intestinal Bacteria ◽  
Relevant Information ◽  
Tenebrio Molitor ◽  
Gut Bacteria ◽  
Ftir Analysis ◽  
Main Carbon Source ◽  
Global Concern ◽  
Main Carbon

Since the 1970s, polystyrene (PS) plastic marine pollution has become a global concern. Tenebrio molitor and Zophobas morio beetle larva actively respond to a diet of primarily polystyrene. The tantalizing evidence indicates that the gut bacteria of these beetle larva enables them not only to consume polystyrene, but to successfully biodegrade polystyrene. Heretofore, data collection to verify polystyrene degradation by the gut bacteria of these larva has taken up to six months per test. Our laboratory created a platform by dissolving PS into a liquid mineral culture medium to examine PS degradation by the gut bacteria. Under investigation, PS works as main carbon source supporting the growth of gut bacteria. Fourier-transform Infrared spectroscopy (FTIR) is an effective method which can provide relevant information on chemical changes. This study presents a methodology using FTIR and Visible Light Spectrometry as an efficient methodology to verify the physiological degradation of polystyrene.

Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmanii

2020 ◽  
Vol 36 (6) ◽  
pp. 78-86
Author(s):  
P.I. Akentyev ◽  
I.I. Gubaidullin ◽  
N.I. Zhiganov ◽  
V.F. Tereshchenkova ◽  
E.N. Elpidina ◽  
...  
Keyword(s):  
Culture Medium ◽  
Polypeptide Chain ◽  
Tenebrio Molitor ◽  
Enzyme Secretion ◽  
Trypsin Treatment ◽  
Yellow Mealworm ◽  
Serine Peptidase

A strain of the Komagataella kurtzmanii yeast, a producer of recombinant peptidase SerP38 from the yellow mealworm Tenebrio molitor, has been obtained. The level of the pro-enzyme secretion was 20-50 mg/L. It was shown that, during secretion in yeast, the target His6-tagged protein was produced in two forms. One of them was a monomer that was efficiently purified by Ni-NTA chromatography and then activated with trypsin. Another form accumulated in the culture medium as oligomers prone to aggregation in the presence of Ni2+ ions and was not activated by trypsin treatment. Aggregation is likely the result of incorrect folding of the polypeptide chain. Tenebrio molitor, S1 family serine peptidase, SerP38, yeast, Komagataella kurtzmanii, ion-dependent aggregation

scholarly journals A β-d-fructofuranosidase from Claviceps purpurea

1972 ◽  
Vol 129 (2) ◽  
pp. 263-272 ◽  
Author(s):  
A. G. Dickerson
Keyword(s):  
Carbon Source ◽  
Dual Role ◽  
Claviceps Purpurea ◽  
Time Transfer ◽  
Main Carbon Source ◽  
Main Carbon

Evidence suggests that sucrose is the main carbon source for growth of Claviceps spp. in the parasitic condition. The sucrose acts as substrate for an active β-fructofuranosidase, produced by the fungus, which in the first instance converts the disaccharide into glucose and an oligofructoside. In this way, 50% of the glucose, supplied as sucrose, is made available to the parasite for assimilation. Subsequent action of the enzyme on both sucrose and the oligofructoside leads to the release of more glucose and the formation of additional oligosaccharides. The structures of the main oligosaccharides formed have been elucidated and the interactions of each compound studied. In experiments with purified enzyme in vitro the interaction of the oligosaccharides is rapid but in culture they are assimilated only slowly; in each case some free fructose is liberated. Free fructose is not assimilated in the presence of glucose and, further, inhibits growth at concentrations which might be expected to occur in the parasitic condition. A dual role has been suggested for the enzyme, with sucrose as substrate, in which glucose is made available to the growing parasite, while at the same time transfer of the fructose to form oligosaccharides prevents it from accumulating at inhibitory concentrations. Ultimately, when glucose becomes limiting, the fungus will adapt to fructose assimilation.

scholarly journals Klebsiella pneumoniae—A Useful Pathogenic Strain for Biotechnological Purposes: Diols Biosynthesis under Controlled and Uncontrolled pH Levels

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 293 ◽  
Author(s):  
Laura Mitrea ◽  
Dan Cristian Vodnar
Keyword(s):  
Klebsiella Pneumoniae ◽  
Ph Value ◽  
Cell Factory ◽  
Anaerobic Conditions ◽  
Liquid Media ◽  
Microbial Cell Factory ◽  
Substrate Consumption ◽  
Main Carbon Source ◽  
Ph Level ◽  
Main Carbon

Despite being a well-known human pathogen, Klebsiella pneumoniae plays a significant role in the biotechnology field, being considered as a microbial cell factory in terms of valuable chemical biosynthesis. In this work, Klebsiella pneumoniae DSMZ 2026 was investigated for its potential to biosynthesize 1,3-propanediol (PDO) and 2,3-butanediol (BDO) during batch fermentation under controlled and uncontrolled pH levels. The bacterial strain was cultivated at a bioreactor level, and it was inoculated in 2 L of specific mineral broth containing 50 g/L of glycerol as the main carbon source. The process was conducted under anaerobic conditions at 37 °C and 180 RPM (rotations per minute) for 24 h. The effect of pH oscillation on the biosynthesis of PDO and BDO was investigated. Samples were taken every 3 h and specific tests were performed: pH measurement, main substrate consumption, PDO and BDO production. The cell morphology was analyzed on both solid and liquid media. After 24 h of cultivation, the maximum concentrations of PDO and BDO were 28.63 ± 2.20 g/L and 18.10 ± 1.10 g/L when the pH value was maintained at 7. Decreased concentrations of PDO and BDO were achieved (11.08 ± 0.14 g/L and 7.35 ± 0.00 g/L, respectively) when the pH level was not maintained at constant values. Moreover, it was identified the presence of other metabolites (lactic, citric, and succinic acids) in the cultivation media at the beginning of the process, after 12 h and 24 h of cultivation.

scholarly journals ʲ-mannanase of Streptomyces lividans 66: cloning and DNA sequence of the manA gene and characterization of the enzyme

1993 ◽  
Vol 290 (3) ◽  
pp. 857-863 ◽  
Author(s):  
N Arcand ◽  
D Kluepfel ◽  
F W Paradis ◽  
R Morosoli ◽  
F Shareck
Keyword(s):  
Dna Sequence ◽  
Wild Type Strain ◽  
Specific Activity ◽  
Streptomyces Lividans ◽  
Gene Coding ◽  
Sds Page ◽  
Main Carbon Source ◽  
Assay Conditions ◽  
Main Carbon

The gene coding for a beta-mannanase was cloned homologously from Streptomyces lividans and its DNA sequence was determined. The fully secreted enzyme was isolated and purified from culture filtrates of the hyperproducing clone S. lividans IAF36 grown in mineral salt media containing galactomannan as the main carbon source. It had a molecular mass of 36 kDa and a specific activity of 876 i.u./mg of protein. Under the assay conditions used, the optimal enzyme activity was obtained at 58 degrees C and a pH of 6.8. The pI was 3.5. The kinetic constants of this mannanase determined with galactomannan as substrate were a Vmax. of 205 i.u./mg of enzyme and a Km of 0.77 mg/ml. Data from SDS/PAGE and Western blotting show that the cloned enzyme was identical to that of the wild-type strain.

scholarly journals Metabolism of Linoleic Acid by Human Gut Bacteria: Different Routes for Biosynthesis of Conjugated Linoleic Acid

2007 ◽  
Vol 189 (6) ◽  
pp. 2566-2570 ◽  
Author(s):  
Estelle Devillard ◽  
Freda M. McIntosh ◽  
Sylvia H. Duncan ◽  
R. John Wallace
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Vaccenic Acid ◽  
Intestinal Bacteria ◽  
Gut Bacteria ◽  
Human Tissues ◽  
Human Gut ◽  
Gram Positive ◽  
Health Promoting

ABSTRACT A survey of 30 representative strains of human gram-positive intestinal bacteria indicated that Roseburia species were among the most active in metabolizing linoleic acid (cis-9,cis-12-18:2). Different Roseburia spp. formed either vaccenic acid (trans-11-18:1) or a 10-hydroxy-18:1; these compounds are precursors of the health-promoting conjugated linoleic acid cis-9,trans-11-18:2 in human tissues and the intestine, respectively.

Structure Elucidation and Characterization of Microbial 2-Tridecyl Sophorosides (Biosurfactants)

2013 ◽  
Vol 68 (11-12) ◽  
pp. 489-498 ◽  
Author(s):  
Verena K. Recke ◽  
Jun B. Park ◽  
Melanie Gerlitzki ◽  
Rudolf Hausmann ◽  
Christoph Syldatk ◽  
...  
Keyword(s):  
Structure Elucidation ◽  
Biosynthetic Pathway ◽  
Candida Bombicola ◽  
Gram Positive Bacteria ◽  
Product Mixture ◽  
Crude Product ◽  
Main Carbon Source ◽  
Hydroxy Groups ◽  
Main Carbon

To produce novel types of sophorose lipids containing an odd number of carbon atoms in the lipophilic moiety, Candida bombicola ATCC 22214 was grown in 500-ml fl ask cultures with glucose as main carbon source, and additionally, 2-tridecanone as co-substrate. After solvent extraction, the crude product mixture was separated into pure fractions, and each fraction was analysed via NMR and mass spectroscopy. This effective strategy generated five new glycolipids, 2-tridecyl sophorosides, which differed in the number of glucose units, and acetyl and hydroxy groups, respectively. Based on these compounds, a proposal for the possible biosynthetic pathway was deduced. Two compounds of the mixture, mono- and diacetylated 2-tridecyl sophorosides, respectively, were able to lower the surface tension of water from 72 mN m-1 to 32 mN m-1 and the interfacial tension between water and nhexadecane from 43 mN m-1 down to 4 and 3 mN m-1. Thus, both compounds possess a very good surfactant behaviour. Moreover, it was observed that the new products inhibit the growth of particular Gram-positive bacteria, and they indicate potential for antitumourpromoting activity.

Screening of biosurfactant-producing Bacillus strains using glycerol from the biodiesel synthesis as main carbon source

2012 ◽  
Vol 35 (6) ◽  
pp. 897-906 ◽  
Author(s):  
M. Sousa ◽  
V. M. M. Melo ◽  
S. Rodrigues ◽  
H. B. Sant’ana ◽  
L. R. B. Gonçalves
Keyword(s):  
Carbon Source ◽  
Biodiesel Synthesis ◽  
Main Carbon Source ◽  
Bacillus Strains ◽  
Main Carbon
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