scholarly journals Stachybotrys Chartarum Secondary Metabolots of Poisonic Microzoquary

2021 ◽  
Vol 01 (08) ◽  
Author(s):  
Jurakulova N.X. ◽  
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Spectroscopic Data ◽  
Gradient Elution ◽  
Crystalline Material ◽  
Stachybotrys Chartarum

Filtered culture fluids of the Stachybotrys chartarum strain were exhaustively extracted with ethyl acetate. The iso lated red residue was subjected to column chro matography (silica gel) with gradient elution, resulting in 2 different fractions. Fraction 1-2 consisted of a crystalline material that showed the same parameters of 1H and 13C as the parameters of staxibotrilaktam and staxibotrilaktam А, a derivative of driman's sesquiterpenoid. The structure proposed for this compound was based on spectroscopic data and no indication of the configuration of stereogenic carbon atoms was obtained. The fungus was produced mainly stachybotrys fully characterized derived sesquiterpenoid of drimana.

scholarly journals ISOLASI SIKLOKOMUNOL DARI DAUN SUKUN Artocarpus altilis (PARKINSON) FOSBERG SERTA AKTIVITASNYA SEBAGAI ANTIKANKER

2014 ◽  
Vol 16 (2) ◽  
pp. 82-86 ◽  
Author(s):  
Chandra Risdian ◽  
Tjandrawati Mozef ◽  
Puspa D.N Lotulung
Keyword(s):  
Liver Cirrhosis ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
1H Nmr ◽  
13C Nmr ◽  
Anticancer Agent ◽  
Ethyl Acetate Extract ◽  
Gradient Elution ◽  
Artocarpus Altilis ◽  
Mcf 7

Daun sukun (Artocarpus altilis) sudah lama dijadikan sebagai obat tradisional untuk mengatasi berbagai penyakit seperti liver cirrhosis, hipertensi, dan diabetes. Ekstrak etil asetat dari daun sukun juga berpotensi menghambat pertumbuhan sel kanker. Beberapa senyawa yang telah diketahui sebagai agen antikanker di dalam ekstrak etil asetat daun sukun adalah golongan geranil flavonoid. Akan tetapi, senyawa pyranoflavoid belum dilaporkan dapat ditemukan pada daun sukun. Untuk itu, penelitian ini bertujuan untuk mengisolasi senyawa pyranoflavonoid dari ekstrak etil asetat daun sukun dan menguji aktivitasnya sebagai antikanker. Daun sukun diekstraksi dengan etanol 70% kemudian dilakukan partisi dengan petroleum eter dan etil asetat. Ekstrak etil asetat yang diperoleh difraksinasi dengan kromatografi kolom silica gel dengan gradient elusi heksan-etil asetat sehingga diperoleh senyawa kristal berwarna kuning, AA3. Senyawa AA3 kemudian diidentifikasi dengan spektrofotometer UV-Vis, LC-MS, 1H-NMR dan 13C-NMR dan diperoleh hasil bahwa senyawa AA3 tersebut adalah siklokomunol yang merupakan golongan pyranoflavonoid. Uji antikanker siklokomunol dengan MCF-7 dan T47D menunjukkan adanya aktivitas antikanker dengan nilai IC50 masing-masing adalah 75.46 µM dan 36.20 µM selama 48 jam.Kata kunci:Artocarpus altilis, siklokomunol, antikanker, MCF-7, T47D. Leaves of breadfruit Artocarpus altilis (Parkinson) Fosberg has long been used as traditional medicine to overcome a variety of diseases such as liver cirrhosis, hypertension, and diabetes. Ethyl acetate extract of the leaves of breadfruit also potentially inhibit the growth of cancer cells. Several compounds have been known as an anticancer agent in the ethyl acetate extract of leaves of breadfruit is belong to geranyl flavonoid group. However, the compound belong to pyranoflavoid group has not been reported can be found on the leaves of breadfruit. Therefore, this study aims to isolate the pyranoflavonoid compound from ethyl acetate extract of leaves of breadfruit and tested as an anticancer agent. Breadfruit leaves were extracted with 70% ethanol and then made a partition with petroleum ether and ethyl acetate. Ethyl acetate extract obtained was fractionated by silica gel column chromatography with gradient elution of hexane-ethyl acetate to obtain a yellow crystalline compound, AA3. AA3 compound was identified by UV-Vis spectrophotometer, LC-MS, 1H-NMR and 13C-NMR and obtained results that AA3 compound is siklokomunol which is belong to pyranoflavonoid group. Anticancer test of siklokomunol with MCF-7 and T47D showed anticancer activity with IC50 values of each are 75.46 µM and 36.20 µM, respectively, for 48 hours.Keywords: Artocarpus altilis, siklokomunol, anticancer, MCF-7, T47D.

Iridoids from the stems of Viburnum erosum

Holzforschung ◽  
2014 ◽  
Vol 68 (7) ◽  
pp. 761-767 ◽  
Author(s):  
Seo-Ji In ◽  
Kyeong-Hwa Seo ◽  
Na-Young Song ◽  
Myoung-Chong Song ◽  
Eun-Mi An ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Column Chromatography ◽  
Spectroscopic Data ◽  
2D Nmr ◽  
Butyl Alcohol ◽  
Chemical Structures ◽  
Etoac Fraction ◽  
C Nmr

Abstract Two new iridoids were isolated and identified to three known ones, namely, loganic acid (2), sweroside (3), and 7-O-tigloylsecologanol (4), from the stems of Viburnum erosum. Dried stems of V. erosum were extracted with 80% methanol (MeOH), and the concentrated extract was partitioned successively with ethyl acetate (EtOAc), n-butyl alcohol (n-BuOH), and H2O. Five compounds were isolated after repeated silica gel (SiO2), octadecyl SiO2 (ODS), and Sephadex LH-20 column chromatography of the EtOAc fraction. The chemical structures of the compounds were determined based on spectroscopic data from 1H- and 13C-NMR, DEPT, and 2D-NMR (gCOSY, gHSQC, gHMBC) data. The new iridoids were identified as 3,7-dihydroxy-8-methyl-cyclopenta[c]pyran-4-carboxylic acid (1) and rel-(1S,5R,9S)-9-ethenyl-1-(β-D-glucopyrinosyloxy)-5,9-dihydro-5-{2-[(2-methylbut-2-enoyl)oxy]ethyl}-1H-pyran-4-carboxylic acid (5) and named viburnin and epi-7-O-tigloylsecologanolic acid, respectively. This is the first report of the isolation of these five iridoids from V. erosum.

Adsorption and desorption of binary mixture of acetone and ethyl acetate on silica gel

2019 ◽  
Vol 197 ◽  
pp. 185-194 ◽  
Author(s):  
Hong Sui ◽  
Jijiang Liu ◽  
Lin He ◽  
Xingang Li ◽  
Ammi Jani
Keyword(s):  
Binary Mixture ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Adsorption And Desorption

scholarly journals Isolation and Structure Elucidation of Soulatro Coumarin From Stem Bark of Calophyllum soulattri Burm F and In Vivo Antiplasmodial Activity by Using Mice Infected by Plasmodium berghei

2011 ◽  
Vol 2 (2) ◽  
pp. 242
Author(s):  
Jamilah Abbas ◽  
Achmad Darmawan ◽  
Syafruddin Syafruddin
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Column Chromatography ◽  
Plasmodium Berghei ◽  
2D Nmr ◽  
Stem Bark ◽  
Antiplasmodial Activity ◽  
Nmr Techniques ◽  
Coumarin Compound

The soulatro coumarin compound was isolated and elucidated from the stem bark of Calophyllum soulattri Burm F, the samples were collected from Jayapura Papua Irian Island in Indonesia. Isolation process was done by maceration at room temperature in methanol, than partitioned in a mixture of n hexane-water (1:1), followed by dichloromethane-water (1:1)  and ethyl acetate-water (1:1). A portion of ethyl acetate extract was subjected to column chromatography over silica gel packed and eluted with n-hexane a gradient of ethyl acetate to 100% followed by CHCl3  in MeOH (20:1, 10 :1, 5:1, 1:1). Fraction  B (CHCl3 in MeOH 20:1) was subjected to column chromatography  over silica gel 300 mesh  and eluted with EtOAc-MeOH mixtures of increasing polarity. Faction with the same Rf valeus were combined and eluted with EtOAc-MeOH  (19:1) showed one spot on TLC. They were combined and evaporated to yield a solid than was recrystallized in mixture of CH2Cl2-methanol to give soulatro coumarin compound. The structure was determinated by spectroscopic analysis, in particular by 1D and 2D NMR techniques, from these spectra data conclution that compound is soulatro coumarin. Antimalarial assay was tested against Plasmodium berghei parasite as in vivo using 18 mices rodent wich was infected by  Plasmodium berghei parasite. The soulatro coumarin  showed activity against P. berghei with dosage 0.0005867 mM/1 kg body weight ; 0.005867 mM/1 kg bw; 0.05867 mM/1 kg bw; 0.5867 mM/1 kg bw 5.867 mM/1 kg bw and 58.67 mM/1 kg bw could inhibite growth rate of parasite = 57.32%; 63.37%; 43.02%; 53.49%; 47.67% respectively.Keywords : Antiplasmodial activity, coumarin, Calophyllum soulattri Burm. F, in vivo, Chloroquine, Plasmodium berghei.

scholarly journals ISOLATION AND IDENTIFICATION OF ANANIXANTHONE FROM ETHYL ACETATE EXTRACT OF ROOT BARK OF SLATRI (Calophyllum soulattri) (CLUSIACEAE))

2016 ◽  
Vol 10 (2) ◽  
pp. 130
Author(s):  
Dindha Ramah Mulia ◽  
Nestri Wulandari ◽  
Muhammad Widyo Wartono
Keyword(s):  
Ethyl Acetate ◽  
Spectroscopic Data ◽  
Ethyl Acetate Extract ◽  
Root Bark ◽  
Isolation And Identification

<p><em></em>A  xanthone,  named  ananixanthone  (1)  has  been  isolated  and  identified  from  the  ethyl acetate  extract of the root barks of  Calophyllum soulattri. Structure of the compound was determined based on spectroscopic data, including UV, IR, NMR 1D, NMR 2D and by comparison with references.</p>

scholarly journals A NEW CYTOTOXIC DOLABELLANE FROM THE INDONESIAN SOFT CORAL Anthelia sp.

2013 ◽  
Vol 13 (3) ◽  
pp. 216-220 ◽  
Author(s):  
Anggia Murni ◽  
Novriyandi Hanif ◽  
Junichi Tanaka
Keyword(s):  
Mass Spectrometry ◽  
Drug Development ◽  
Ethyl Acetate ◽  
Spectroscopic Data ◽  
Soft Coral ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Cancer Drug ◽  
Nmr Data ◽  
Anti Cancer

One new dolabellane (1) and two known diterpenoids stolonidiol (2) and clavinflol B (3) have been isolated from the ethyl acetate extract of the Indonesian soft coral Anthelia sp. A new compound 1 exhibited a moderate cytotoxicity against NBT-T2 cells at 10 µg/mL, while known compounds 2 and 3 showed cytotoxicity at 1 and 0.5 µg/mL, respectively. Structure of the new compound 1 was elucidated by interpretation of NMR spectroscopic data (1D and 2D NMR data) and mass spectrometry (ESIMS data) as well as comparison with those of related ones. This finding should be useful for anti cancer drug development of the promising dolabellane-types compound.

scholarly journals Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 359 ◽  
Author(s):  
Prawez Alam ◽  
Essam Ezzeldin ◽  
Muzaffar Iqbal ◽  
Gamal A.E. Mostafa ◽  
Md. Khalid Anwer ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Solid Lipid Nanoparticles ◽  
Ternary Mixture ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Lipid Nanoparticles ◽  
Ammonia Solution ◽  
Hptlc Method

In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using “RP-18 silica gel 60 F254S HPTLC plates”. However; NP-HPTLC estimation of DLFX was performed using “silica gel 60 F254S HPTLC plates”. For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 v/v/v) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4:2 v/v/v) was used as normal mobile phase. The analysis of DLFX was conducted in absorbance/reflectance mode of densitometry at λmax = 295 nm for both methods. RP-HPTLC method was found more accurate, precise, robust and sensitive for the analysis of DLFX compared with the NP-HPTLC method. The % assay of DLFX in commercial tablets and in-house developed SLNs was determined as 98.2 and 101.0%, respectively, using the green RP-HPTLC technique, however; the % assay of DLFX in commercial tablets and in-house developed SLNs was found to be 94.4 and 95.0%, respectively, using the NP-HPTLC method. Overall, the green RP-HPTLC method was found superior over the NP-HPTLC. Therefore, the proposed green RP-HPTLC method can be successfully applied for analysis of DLFX in commercial tablets, SLNs and other formulations containing DLFX.

scholarly journals THE ANTIBACTERIAL ACTIVITY OF FRACTIONS OF ETHYL ACETATE GARCINIA LATISSIMA MIQ. STEM BARK EXTRACTS AGAINST BACILLUS SUBTILIS AND PSEUDOMONAS AERUGINOSA

2017 ◽  
Vol 10 (17) ◽  
pp. 69
Author(s):  
Neneng Sitisilfi Ambarwati ◽  
Amarila Malik ◽  
Elfira Amalia Deborah ◽  
Cakra Hagai Arpatism ◽  
Maulfi Hanif ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Mobile Phase ◽  
Agar Medium ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Tetrazolium Salt ◽  
Inhibitory Zone ◽  
Zone Diameter

 Objective: This research focuses on the antibacterial activities of fractions of ethyl acetate Garcinia latissima Miq. extracts against Bacillus subtilis and Pseudomonas aeruginosa.Methods: For each bacteria, the values of the inhibitory zone diameter were determined using paper discs method, while the minimum inhibitory concentration (MIC) was determined by microdilution and antibacterial assay was determined by bioautography. Fractionation was carried out using gradient elution with silica gel column as stationary phase successively increasing the separation according to the polarity (using combination of eluents; n-hexane, ethyl acetate, and methanol).Results: To determine the diameter of the inhibitory zone for each fraction, the fractions were diluted with dimethyl sulfoxide until the concentration of each fraction was 20,000 ppm. The results showed that fraction B had no inhibitory zone against B. subtilis. The inhibitory zone diameters of fractions A, C, D, E, F, and G against B. subtilis were 7.600±0.000, 6.767±0.202, 7.950±0.477, 7.883±0.901, and 9.233±0.231 mm, respectively. Only Fraction G had an inhibition zone diameter of 7.200±0.173 mm against P. aeruginosa. The active fractions were tested by contact bioautography using silica gel (60 GF254) plate and hexane:chloroform (2:3) as mobile phase for Fractions A, C, D, E, and F and chloroform:ethyl acetate (4:1) as mobile phase for Fraction G. The thin layer chromatography plates were observed under the ultraviolet light at wavelengths of 254 and 366 nm, while the others plate (using in contact bioautography) placing on agar medium that had been inoculated with the bacteria, so the compounds could be diffuse to the medium. The active compound will appeared inhibitory zones in agar medium. The determination of the MIC values using microdilution with methylthiazol tetrazolium salt showed that fractions of the ethyl acetate extracts of G. latissima Miq. stem bark inhibited the growth of B. subtilis more actively than P. aeruginosa. The highest activity of the fractions against B. subtilis was shown by fraction G, which had a MIC value of 78.125 ppm, followed by fractions F (156.25 ppm), E (312.5 ppm), D (625 ppm), C (1250 ppm), B (2500 ppm), and A (5000 ppm). The highest activity of the fractions against P. aeruginosa was Fraction G (5000 ppm), while the MIC values of other fractions were >5000 ppm.Conclusion: Further investigations should be conducted to obtain the new antibacterial compounds in fractions of ethyl acetate extract of G. latissima Miq. stem bark that effective to inhibit the growth of B. subtilis and P. aeruginosa.

Quantitative Determination of Tris-(2,3,-dibromopropyl) Phosphate by Densitometry of Thin Layer Chromatograms

1979 ◽  
Vol 62 (6) ◽  
pp. 1315-1318
Author(s):  
Albert M Gardner
Keyword(s):  
Sewage Sludge ◽  
Thin Layer ◽  
Quantitative Determination ◽  
Ethyl Acetate ◽  
Silver Nitrate ◽  
Flame Retardant ◽  
Silica Gel ◽  
White Background ◽  
Ppm Level

Abstract The flame retardant tris-(2,3-dibromopropyl) phosphate (Tris) was chromatographed on silica gel thin layer plates, using ethyl acetate-hexane (30+70) as the developing solvent. Tris was visualized by spraying the chromatogram with 1% aqueous silver nitrate followed by exposure to ultraviolet light for 40 min. Tris appeared as dark gray spots on a white background; the spots were quantitated by densitometry at 600 nm. The lower level of sensitivity was 50 ng; calibration plots were linear from 50 to 800 ng. The procedure was developed to determine the levels of unmetabolized Tris remaining after exposure to sewage sludge suspensions. Recovery of Tris from 3 sewage sludge samples fortified at the 1.0 ppm level averaged 97%.

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