scholarly journals A NEW CYTOTOXIC DOLABELLANE FROM THE INDONESIAN SOFT CORAL Anthelia sp.

2013 ◽  
Vol 13 (3) ◽  
pp. 216-220 ◽  
Author(s):  
Anggia Murni ◽  
Novriyandi Hanif ◽  
Junichi Tanaka
Keyword(s):  
Mass Spectrometry ◽  
Drug Development ◽  
Ethyl Acetate ◽  
Spectroscopic Data ◽  
Soft Coral ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Cancer Drug ◽  
Nmr Data ◽  
Anti Cancer

One new dolabellane (1) and two known diterpenoids stolonidiol (2) and clavinflol B (3) have been isolated from the ethyl acetate extract of the Indonesian soft coral Anthelia sp. A new compound 1 exhibited a moderate cytotoxicity against NBT-T2 cells at 10 µg/mL, while known compounds 2 and 3 showed cytotoxicity at 1 and 0.5 µg/mL, respectively. Structure of the new compound 1 was elucidated by interpretation of NMR spectroscopic data (1D and 2D NMR data) and mass spectrometry (ESIMS data) as well as comparison with those of related ones. This finding should be useful for anti cancer drug development of the promising dolabellane-types compound.

scholarly journals An α-Sophoradiol Glycoside from the Root Wood of Erythrina senegalensis DC. (Fabaceae) with α-Amylase and α-Glucosidase Inhibitory Potential

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110445
Author(s):  
Cyrille Tchuente Djoko ◽  
Isaac Silvère Gade ◽  
Alex De Theodore Atchade ◽  
Alfred Ngenge Tamfu ◽  
Rodica Mihaela Dinica ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ethyl Acetate ◽  
Spectroscopic Data ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Phytochemical Study ◽  
Inhibitory Potential

Phytochemical study of the roots of Erythrina senegalensis led to the isolation of a new α-sophoradiol glycoside, erythrinoside (1), together with four known compounds, lupeol (2), α-sophoradiol (3), isoneorautenol (4) and D-mannitol (5). The structures of the compounds were elucidated using spectroscopic data including 1D and 2D NMR, mass spectrometry and by comparison made with some data reported previously; the samples (extracts and compounds) were also subjected to antidiabetic assay. Erythrinoside and isoneorautenol exhibited good α-amylase inhibitory potential of 54.6% and 53.3%, respectively, compared to acarbose (72.5%) at 400 µg/mL. With α-glucosidase, all samples showed promising inhibition percentages above 50% at 200 µg/mL. In the α-glucosidase assay, the ethyl acetate extract (65.5%), methanol extract (72.1%), erythrinoside (63.3%) and isoneorautenol (66.0%) had percentage inhibitions closer to that of acarbose (69.0%) at 200 µg/mL. The methanol extract (IC50 = 81.2 ± 0.9 µg/mL) was more active than acarbose (IC50 = 94.5 ± 0.7 µg.mL) in the α-glucosidase assay. The inhibition of α-amylase and α-glucosidase indicates that E. senegalensis extracts and compounds could be used to manage diabetic conditions.

scholarly journals Four flavonols from the seed of Hibiscus sabdariffa. Linn. growing in Binh Thuan Province

2019 ◽  
Vol 22 (4) ◽  
pp. 348-351
Author(s):  
Pham Nguyen Kim Tuyen ◽  
Truong Van Tien ◽  
Tran The Anh Huy ◽  
Nguyen Thi Hoa ◽  
Huynh Bui Linh Chi ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Hibiscus Sabdariffa ◽  
Asian Countries ◽  
Chromatography Analysis ◽  
Liquid Liquid Extraction ◽  
Nmr Data ◽  
First Time ◽  
Layer Chromatography

Introduction: Hibiscus sabdariffa Linn. (Malvaceae) is a medicinal plant popularly distributed in Asian countries. As of 2019, no chemical investigations from the Vietnamese plant were found. This paper reported the isolation and elucidation of compounds isolated from the seeds of Hibiscus sabdariffa Linn. (Malvaceae) growing in Binh Thuan province. Method: The maceration was applied to the seeds of the plant to afford the crude extract which was then fractionated by liquid-liquid extraction to obtain the ethyl acetate extract. The extract was carried out by using normal phase silica gel column chromatography and thin-layer chromatography. Analysis of spectroscopic data including HR-ESI-MS, 1D and 2D-NMR and a comparison of the NMR data with that in the literature led to the structural elucidation of isolated compounds. Results: Four flavonols comprising quercetin 3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (1), quercetin 3-O-β-D-glucopyranoside (2), quercetin (3) and kaempferol (4) were isolated from the ethyl acetate extract. Conclusions: This is the first time the isolated compounds were found from the seeds of this plant.

scholarly journals Thiocarbamates from Moringa Oleifera

2006 ◽  
Vol 1 (9) ◽  
pp. 1934578X0600100 ◽  
Author(s):  
Amit Tewari ◽  
Rajendra S. Bhakuni
Keyword(s):  
Ethyl Acetate ◽  
Spectroscopic Data ◽  
Moringa Oleifera ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Ms Analysis ◽  
New Compounds

Two new thiocarbamates (1 and 2) along with two known compounds have been isolated from the ethyl acetate extract of Moringa oleifera seeds. The new compounds were characterized as O-n-butyl-4-[(α-L-rhamnopyranosyloxy)benzyl] thiocarbamate (E) (1) and O-ethyl-4–[(α-L-rhamnopyranosyloxy)–3-hydroxybenzyl]thiocarbamate (E) (2) on the basis of 1D and 2D NMR spectroscopic data and MS analysis.

scholarly journals Bioactive Diketopiperazines and Nucleoside Derivatives from a Sponge-Derived Streptomyces Species

Marine Drugs ◽  
2019 ◽  
Vol 17 (10) ◽  
pp. 584 ◽  
Author(s):  
Lamiaa A. Shaala ◽  
Diaa T. A. Youssef ◽  
Jihan M. Badr ◽  
Steve M. Harakeh ◽  
Grégory Genta-Jouve
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Antimicrobial Activities ◽  
Isolation And Identification ◽  
Streptomyces Species ◽  
Mass Spectral ◽  
Nmr Data ◽  
The Absolute

Fractionation and purification of the ethyl acetate extract of the culture of a sponge-derived actinomycete, Streptomyces species Call-36, resulted in the isolation and identification of a new diketopiperazine, actinozine A (1), cyclo(2-OH-d-Pro-l-Leu) (2), two new nucleosides, thymidine-3-mercaptocarbamic acid (3) and thymidine-3-thioamine (4), together with cyclo(d-Pro-l-Phe) (5) and cyclo(l-Pro-l-Phe) (6). The structure assignments of the compounds were carried out by interpretation of 1D and 2D NMR data and mass spectral determinations. The absolute configurations of 1 and 2 were determined by Marfey’s method and by comparison of the experimental and TDDFT-calculated ECD spectra. Actinozine A possesses an unprecedented hydroperoxy moiety at C-2 of the proline moiety, while 3 and 4 possess unusual mercaptocarbamic acid and thiohydroxylamine functionalities at N-3 of the thymine moiety. The isolated compounds displayed variable cytotoxic and antimicrobial activities.

scholarly journals IN VITRO CYTOTOXIC AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDIES ON ORTHOSIPHON STAMINEUS BENTH. (LEAF) AGAINST MCF–7 CELL LINES

2017 ◽  
Vol 10 (3) ◽  
pp. 129
Author(s):  
Renuka Saravanan ◽  
Brindha Pemiah ◽  
Mahesh Narayanan ◽  
Sivakumar Ramalingam
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Orthosiphon Stamineus ◽  
Diphenyltetrazolium Bromide ◽  
Anti Cancer ◽  
Mcf 7

ABSTRACTObjective: The objective of the study was to determine the anticancer efficacy of Orthosiphon stamineus extract against Michigan Cancer Foundation-7(MCF-7) and its phytochemical analysis through gas chromatography-mass spectrometry (GC-MS).Methods: Different solvents were used for leaf extraction and used for qualitative assay of phytochemicals using standard protocols. Differentconcentration (12.5, 25, 50, 100, and 200 μg/ml) of methanol extract and ethyl acetate extract of O. stamineus leaves were used to assess the in vitrocytotoxic effect using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Further, the ethyl acetate extract was subjected toGC-MS analysis, and the identification of components was based on the National Institute of Standards and Technology library database.Result: Of the hexane, methanol and ethyl acetate extracts, methanol extract found to contain more of phytochemicals followed by ethyl acetate. Theinhibitory concentration 50 for methanol extract and ethyl acetate extract was found be 93.42 μg/ml and 215.3 μg/ml, respectively.GC-MS mass spectrum of ethyl acetate extract revealed the presence of squalene and phytol and antioxidants such as flavones. Sterols compoundssuch as vitamin E, ergosterol, cholesterol, and 3,7,11,15-tetramethyl-2-hexadecen-1-ol and terpenoids were also identified.Conclusion: The data obtained in this work could be useful as a chemical standard in checking the genuineness of this plant source. Data of the resultsfurther depicted that the selected traditional anti-cancer plants could be used not only as an anti-cancer but also as a good antioxidant.Keywords: Anti-cancer, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Orthosiphon stamineus, Michigan cancer fsoundation-7cells.

Termiglaucescin, a new polyhydroxy triterpene glucoside from Terminalia glaucescens with antioxidant and anti-inflammatory potential

2017 ◽  
Vol 72 (5-6) ◽  
pp. 203-208
Author(s):  
Amadou Dawé ◽  
Benjamin Talom ◽  
Gilbert Deccaux Wabo Fotso Kapche ◽  
Kauser Siddiqui ◽  
Fawai Yakai ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Spectroscopic Data ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Root Bark ◽  
Anti Inflammatory ◽  
First Time

Abstract Termiglaucescin (1), a new triterpene glucoside, has been isolated from the ethyl acetate extract of the root bark of Terminalia glaucescens Planch. ex Benth, together with 11 known compounds, β-D-glucopyranosyl 2α,3β,6β-trihydroxy-23-galloylolean-12-en-28-oate (2), arjunglucoside I (3), sericoside (4), arjungenin (5), sericic acid (6), arjunetin (7), chebuloside II (8), 3,3′,4-tri-O-methylelagic acid (9), 3,3′-di-O-methylelagic acid (10), β-sitosterol (11) and stigmasterol (12). Compounds 2, 3, 7, 8 and 9 are reported from the plant for the first time. The structures of the isolated compounds were characterized by spectroscopic data interpretations, especially 1D and 2D NMR. The triterpenic isolates showed potent antioxidant and anti-inflammatory activities.

ANTI-CANCER COMPOUNDS ISOLATED FROM SOFT CORAL SINULARIA CRUCIATA - ALCYONIIDAE

2012 ◽  
pp. 84-89
Author(s):  
Quoc Hung Vo ◽  
Nguyen Phuong Nhi Doan ◽  
Dinh Quynh Phu Nguyen ◽  
Thi Dieu Tram Ho ◽  
Thi Hoai Nguyen
Keyword(s):  
Mass Spectrometry ◽  
Soft Coral ◽  
Normal Phase ◽  
Ionization Mass ◽  
Medical Field ◽  
Bioactive Substances ◽  
Anti Cancer ◽  
The World ◽  
Pure Compounds ◽  
Layer Chromatography

Objectives: Nowadays, bioactive substances isolated from marine organisms which are abundant and varied in Vietnamese sea attracted more and more the attention of scientists in the world and Vietnam as well. We have studied on soft coral Sinularia cruciata – Alcyoniidae, which has never been studied in Vietnam before, to find substances which are useful in medical field, especially in anti-cancer therapy. Materials and method: Specimens of soft coral Sinularia cruciata were collected from Con Co, Quang Tri province in May 2011. Pure compounds were isolated by using Thin Layer Chromatography; Column Chromatography normal phase and inverse phase; Shephadex LH 20. Structures of them were determined by spectral data of Nuclear Magnetic Resonance (NMR), Electrospray Ionization Mass Spectrometry (ESI-MS). Results & Conclusion: Structures of 4 compounds were identified: (1) 5.8-epidioxycholest-6-en-3-ol (2) Cholesterol (3) 1-O-hexadecyl-glycerol (Chimyl alcohol) (4) Glycerol 1-O-octadecyl ether (Batyl alcohol). The substance (1) was demonstrated to have strong anti-cancer effects in previous study. Key words Sinularia cruciata, Alcyoniidae, 5,8-epidioxycholest-6-en-3-ol, soft coral, cancer.

Diverse thiophenes as scaffolds in anti-cancer drug development: A concise review

2020 ◽  
Vol 20 ◽  
Author(s):  
Neha V. Bhilare ◽  
Pratibha B. Auti ◽  
Vinayak S. Marulkar ◽  
Vilas J. Pise
Keyword(s):  
Drug Development ◽  
Anticancer Agents ◽  
Heterocyclic Ring ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Cancer Drug ◽  
Active Compounds ◽  
Natural Origin ◽  
Concise Review ◽  
Anti Cancer

: Thiophenes are one among the abundantly found heterocyclic ring systems in many biologically active compounds. Moreover various substituted thiophenes exert numerous pharmacological actions on account of their isosteric resemblance with compounds of natural origin thus rendering them with diverse actions like antibacterial, antifungal, antiviral, anti-inflammatory, analgesic, antiallergic, hypotensives etc.. In this review we specifically explore the chemotherapeutic potential of variety of structures consisting of thiophene scaffolds as prospective anticancer agents.

Prevascularized, Co-Culture Model for Breast Cancer Drug Development

2012 ◽  
Author(s):  
Lauren Marshall ◽  
Isabel Löwstedt ◽  
Paul Gatenholm ◽  
Joel Berry
Keyword(s):  
Breast Cancer ◽  
Drug Development ◽  
Three Dimensional ◽  
Human Tumor ◽  
3D Models ◽  
Cancer Drug ◽  
Cancer Therapies ◽  
Anti Cancer

The objective of this study was to create 3D engineered tissue models to accelerate identification of safe and efficacious breast cancer drug therapies. It is expected that this platform will dramatically reduce the time and costs associated with development and regulatory approval of anti-cancer therapies, currently a multi-billion dollar endeavor [1]. Existing two-dimensional (2D) in vitro and in vivo animal studies required for identification of effective cancer therapies account for much of the high costs of anti-cancer medications and health insurance premiums borne by patients, many of whom cannot afford it. An emerging paradigm in pharmaceutical drug development is the use of three-dimensional (3D) cell/biomaterial models that will accurately screen novel therapeutic compounds, repurpose existing compounds and terminate ineffective ones. In particular, identification of effective chemotherapies for breast cancer are anticipated to occur more quickly in 3D in vitro models than 2D in vitro environments and in vivo animal models, neither of which accurately mimic natural human tumor environments [2]. Moreover, these 3D models can be multi-cellular and designed with extracellular matrix (ECM) function and mechanical properties similar to that of natural in vivo cancer environments [3].

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