Determination of Antioxidant Compounds, Proximate Compositions and Assessment of Free Radical Scavenging Activities of Nypa Fruticans Wurmb. Sap

Nipa palm sap (NPS) as source of medicine traditionally used to treat various diseases. This study identified good radical scavenging activity in NPS with the IC50 value of 33.36 μg/mL using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. NPS comprises of moisture (72.44%), ash (1.04%), protein (7.04%), carbohydrate (19.48%), fat (0%), and energy level (106 kcal). Glucose (0.3%) and fructose (1.8%) were detected using high-performance liquid chromatography. Maleic acid, cinnamic acid, chlorogenic acid, and kaempferol were the predominant compounds revealed by ultra-high-performance liquid chromatography. Overall, NPS has the potential antioxidants sources with significant health benefits and values for commercialisation.

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Identification of Phenolic Acids and Flavonoids in Monofloral Honey from Bangladesh by High Performance Liquid Chromatography: Determination of Antioxidant Capacity

BioMed Research International ◽

10.1155/2014/737490 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 20

Author(s):

Mohammed Moniruzzaman ◽

Chua Yung An ◽

Pasupuleti Visweswara Rao ◽

Mohammad Nurul Islam Hawlader ◽

Siti Amirah Binti Mohd Azlan ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Phenolic Acids ◽

High Performance ◽

Radical Scavenging Activity ◽

Sugar Content ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Total Phenolic ◽

The Mean

The aim of the present study was to characterize the phenolic acids, flavonoids, and antioxidant properties of monofloral honey collected from five different districts in Bangladesh. A new high performance liquid chromatography (HPLC) equipped with a UV detector method was developed for the identification of the phenolic acids and flavonoids. A total of five different phenolic acids were identified, with the most abundant being caffeic acid, benzoic acid, gallic acid, followed by chlorogenic acid and trans-cinnamic acid. The flavonoids, kaempferol, and catechin were most abundant, followed by myricetin and naringenin. The mean moisture content, total sugar content, and color characteristics of the honey samples were 18.36 ± 0.95%, 67.40 ± 5.63 g/100 g, and 129.27 ± 34.66 mm Pfund, respectively. The mean total phenolic acids, total flavonoid content, and proline content were 199.20 ± 135.23, 46.73 ± 34.16, and 556.40 ± 376.86 mg/kg, respectively, while the mean FRAP values and DPPH radical scavenging activity were 327.30 ± 231.87 μM Fe (II)/100 g and 36.95 ± 20.53%, respectively. Among the different types of honey, kalijira exhibited the highest phenolics and antioxidant properties. Overall, our study confirms that all the investigated honey samples are good sources of phenolic acids and flavonoids with good antioxidant properties.

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Chromatographic and Spectroscopic Fingerprinting of Ficus carica and Evaluation of In Vitro Antioxidant Activity

International Journal of Agriculture and Biology ◽

10.17957/ijab/15.1716 ◽

2021 ◽

Vol 25 (03) ◽

pp. 677-682

Author(s):

Amara Javaid

Keyword(s):

Antioxidant Activity ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Antioxidant Potential ◽

Ficus Carica ◽

In Vitro Antioxidant Activity

The present study was conducted to evaluate the in vitro antioxidant activity of Ficus carica, commonly known as fig. Methanol and ethanol extracts of F. carica leaves were subjected to 2, 2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity assay where ascorbic acid being positive control had an IC50 value of 3.98±0.26 while methanol and ethanol fractions showed an IC50 of 101.76±1.12 and 93.12±1.17 respectively exhibiting their high antioxidant potential. DPPH assay was also performed on high performance liquid chromatography (HPLC) elutions. Most active antioxidant components in ethanol extract were eluted between 17–18 min, and those in methanol were eluted over 14–15 min and upon ultra-high performance liquid chromatography-mass spectrometery (Orbitrap Liquid Chromatography-Mass Spectrometry) were identified to be 13-Docosenamide, (Z)- for ethanol and ficusin for methanol fraction. Thus, it is concluded that these two components are most probable determinants of antioxidant potential of F. carica leaf extracts. © 2021 Friends Science Publishers

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