scholarly journals Determination of macroline and tetracyclines group in water by liquid chromatography tandem mass spectrometry (LC-MS/MS)

2020 ◽  
Vol 3 (3) ◽  
pp. 173-182
Author(s):  
Trong Le Van ◽  
Thanh Hang Nguyen Thi ◽  
Huy Do Quang ◽  
Khai Nguyen Manh ◽  
◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Accuracy ◽  
Method Detection Limit ◽  
Chromatography Tandem Mass Spectrometry ◽  
Low Concentrations ◽  
Liquid Chromatography Tandem Mass ◽  
Trace Concentrations ◽  
Positive Ion ◽  
Tetracycline Group

Antibiotics are contaminants at low concentrations and found in water with the range of ng/L. Determination of antibiotics at trace concentrations requires modern and high accuracy equipment. Liquid chromatography mass tandem spectrometry (LC-MS/MS) able to determine contaminants at ppt and ppb levels, which is suitable for quantification of the macrolide and tetracycline group in water. The Analytical method using Agilent Triple Quard 6460 LC-MS/MS system with positive ion mode. Agilent XDB C18 column (1.8 µm × 2.1 × 100 mm) and Agilent Eclipse SDB C18 pre-column (2.1 × 5 mm × 1.8 µm) were used. Mobile phase was acetonitrile and HCOOH 0.1%. Samples were cleaned with Oasis PRiME HLB 3cc SPE column (150 mg). The method was evaluated based on specificity, recovery, repeatability and estimation of the uncertainty. The method detection limit (MDL) for the analytes is 0.03 µg/L. Recovery ranges from 85.23 -117.70%; repeatability (RSDr) is between 2.20% and 12.45%.

scholarly journals Determination of Nitroimidazole Residues in Porcine Urine by Liquid Chromatography/TandemMass Spectrometry

2006 ◽  
Vol 89 (4) ◽  
pp. 1116-1119 ◽  
Author(s):  
Xi Xia ◽  
Xiaowei Li ◽  
Jianzhong Shen ◽  
Suxia Zhang ◽  
Shuangyang Ding ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Mobile Phase ◽  
Solid Phase ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Mass Spectrometry System ◽  
Ionization Mode ◽  
Positive Ion ◽  
Mode A

Abstract A reliable and sensitive method was developed for determination of nitroimidazoles in porcine urine. Sample preparation involves an extraction with ethyl acetate, followed by a solid-phase extraction cleanup step. The final extract is injected into the liquid chromatography/tandem mass spectrometry system in the positive-ion electrospray ionization mode. A C8 column with water and acetonitrile as the mobile phase is used for chromatographic separation under gradient conditions. Overall average recoveries ranged from 83 to 107%. The limits of detection ranged from 0.03 to 0.05 ng/mL, and the limits of quantitation, from 0.1 to 0.2 ng/mL.

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