Endophytic bacteria in grass crop growth promotion and biostimulation

Black pepper (Piper nigrum L.) is one of the most important crops and global demand continues to increase, giving it a high export value. However, black pepper cultivation has been seriously affected by a number of pathogenic diseases. Among them, “quick wilt” caused by Phytophthora sp., “slow decline” caused by Fusarium sp., and root-knot nematode Meloidogyne sp. have a serious negative effect on black pepper growth and productivity. There have been different chemical and biological methods applied to control these diseases, but their effectiveness has been limited. The aim of this research was to evaluate different combinations of rhizosphere bacteria and endophytic bacteria isolated from black pepper farms in the Central Highland of Vietnam for their ability to suppress pathogens and promote black pepper growth and yield. Formula 6, containing the strains Bacillus velezensis KN12, Bacillus amyloliquefaciens DL1, Bacillus velezensis DS29, Bacillus subtilis BH15, Bacillus subtilis V1.21 and Bacillus cereus CS30 exhibited the largest effect against Phytophthora and Fusarium in the soil and in the roots of black pepper. These bio-products also increased chlorophyll a and b contents, which led to a 1.5-fold increase of the photosynthetic intensity than the control formula and a 4.5% increase in the peppercorn yield (3.45 vs. 3.30 tons per hectare for the control). Our results suggest that the application of rhizosphere and endophytic bacteria is a promising method for disease control and growth-promotion of black pepper.

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Isolation of phosphate solubilizing endophytic bacteria from Phyllanthus amarus Schum & Thonn: Evaluation of plant growth promotion and antioxidant activity under salt stress

Journal of Applied Research on Medicinal and Aromatic Plants ◽

10.1016/j.jarmap.2016.02.003 ◽

2016 ◽

Vol 3 (2) ◽

pp. 71-77 ◽

Cited By ~ 17

Author(s):

Manoharan Melvin Joe ◽

Shalini Devaraj ◽

Abitha Benson ◽

Tongmin Sa

Keyword(s):

Antioxidant Activity ◽

Salt Stress ◽

Plant Growth ◽

Plant Growth Promotion ◽

Endophytic Bacteria ◽

Growth Promotion ◽

Phyllanthus Amarus ◽

Phosphate Solubilizing

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Potential of root nodule non‐rhizobial endophytic bacteria for growth promotion of Lotus corniculatus L. and Dactylis glomerata L.

Journal of Applied Microbiology ◽

10.1111/jam.15152 ◽

2021 ◽

Author(s):

Magdalena Knežević ◽

Tanja Berić ◽

Aneta Buntić ◽

Dušica Delić ◽

Ivan Nikolić ◽

...

Keyword(s):

Endophytic Bacteria ◽

Root Nodule ◽

Growth Promotion ◽

Dactylis Glomerata ◽

Lotus Corniculatus ◽

Dactylis Glomerata L

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Lab and Field Level Potential of Endophytic Bacteria in Managing the Dry Root Rot Disease in Black Gram Incited by Rhizoctonia bataticola (Taubenh) Butler

Legume Research - An International Journal ◽

10.18805/lr-4522 ◽

2021 ◽

Author(s):

N. Kiran Kumar ◽

P. Nagamani ◽

K. Viswanath ◽

L. Prasanthi

Keyword(s):

Root Rot ◽

Protease Activity ◽

Endophytic Bacteria ◽

Phosphate Solubilization ◽

Growth Promotion ◽

Dry Weight ◽

Black Gram ◽

Rhizoctonia Bataticola

Background: The plant growth promotion and efficacy against phytopathogens by the endophytic bacteria are being focused now due to their ecofriendly nature. Methods: Endophytic bacteria (24 nos) isolated from the roots, stems and leaves of black gram plants collected from different locations were tested for their potential to inhibit the growth of R. bataticola under in vitro and in vivo conditions. Result: The leaf endophyte BLE 4 exhibited maximum inhibition (79.6%) of R. bataticola followed by BSE 4 (77.4%), BSE 7 (77.0%) and BLE 1 (74.0%). Among these 4 isolates tested as seed treatment and soil application, there was significant increase in dry weight (7.1 g), plant height (37.7 cm), number of branches (13.2) and number of pods (26.2) in BLE 4 treated plots. Whereas, the incidence of dry root rot and yield were insignificant. In the screening study of isolates for their phosphate solubilization potential, protease activity, siderophore and HCN production, no single isolate possessed all the properties, but siderophore production, protease activity and phosphate solubilization were found in BSE 4, BRE 3, BRE 5 and BRE 10 isolates.

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Root-Associated Endophytic Bacterial Community Composition of Pennisetum sinese from Four Representative Provinces in China

Microorganisms ◽

10.3390/microorganisms7020047 ◽

2019 ◽

Vol 7 (2) ◽

pp. 47 ◽

Cited By ~ 6

Author(s):

Zhen-Shan Deng ◽

Bao-Cheng Zhang ◽

Xiang-Ying Qi ◽

Zhi-Hong Sun ◽

Xiao-Long He ◽

...

Keyword(s):

Bacterial Community ◽

Community Composition ◽

Endophytic Bacteria ◽

Growth Promotion ◽

Bacterial Community Composition ◽

Shannon Index ◽

Plant Production ◽

Total Carbon ◽

Shaanxi Province ◽

Endophytic Bacterial Community

Pennisetum sinese, a source of bio-energy with high biomass production, is a species that contains high crude protein and will be useful for solving the shortage of forage grass after the implementation of “Green for Grain” project in the Loess plateau of Northern Shaanxi in 1999. Plants may receive benefits from endophytic bacteria, such as the enhancement of plant growth or the reduction of plant stress. However, the composition of the endophytic bacterial community associated with the roots of P. sinese is poorly elucidated. In this study, P. sinese from five different samples (Shaanxi province, SX; Fujian province, FJ; the Xinjiang Uyghur autonomous prefecture, XJ and Inner Mongolia, including sand (NS) and saline-alkali land (NY), China) were investigated by high-throughput next-generation sequencing of the 16S rDNA V3-V4 hypervariable region of endophytic bacteria. A total of 313,044 effective sequences were obtained by sequencing five different samples, and 957 effective operational taxonomic units (OTUs) were yielded at 97% identity. The phylum Proteobacteria, the classes Gammaproteobacteria and Alphaproteobacteria, and the genera Pantoea, Pseudomonas, Burkholderia, Arthrobacter, Psychrobacter, and Neokomagataea were significantly dominant in the five samples. In addition, our results demonstrated that the Shaanxi province (SX) sample had the highest Shannon index values (3.795). We found that the SX (308.097) and NS (126.240) samples had the highest and lowest Chao1 richness estimator (Chao1) values, respectively. Venn graphs indicated that the five samples shared 39 common OTUs. Moreover, according to results of the canonical correlation analysis (CCA), soil total carbon, total nitrogen, effective phosphorus, and pH were the major contributing factors to the difference in the overall composition of the bacteria community in this study. Our data provide insights into the endophytic bacteria community composition and structure of roots associated with P. sinese. These results might be useful for growth promotion in different samples, and some of the strains may have the potential to improve plant production in future studies.

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Endophytic bacteria of desert cactus (Euphorbia trigonas Mill) confer drought tolerance and induce growth promotion in tomato (Solanum lycopersicum L.)

Microbiological Research ◽

10.1016/j.micres.2019.126302 ◽

2019 ◽

Vol 228 ◽

pp. 126302 ◽

Cited By ~ 5

Author(s):

Pierre Eke ◽

Aundy Kumar ◽

Kuleshwar Prasad Sahu ◽

Louise Nana Wakam ◽

Neelam Sheoran ◽

...

Keyword(s):

Drought Tolerance ◽

Solanum Lycopersicum ◽

Endophytic Bacteria ◽

Growth Promotion ◽

Solanum Lycopersicum L

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Interactions between putatively endophytic bacteria and tall fescue (Festuca arundinacea): plant growth promotion and colonization in host and non-host cultivars

Plant and Soil ◽

10.1007/s11104-019-04359-5 ◽

2019 ◽

Vol 451 (1-2) ◽

pp. 207-220

Author(s):

Patricia Vaz Jauri ◽

Cecilia Taulé ◽

Maria Cecilia de los Santos ◽

Belén Fernandez ◽

Andrés Di Paolo ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Tall Fescue ◽

Festuca Arundinacea ◽

Endophytic Bacteria ◽

Growth Promotion

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Biocontrol of Fusarium wilt and growth promotion of tomato plants using endophytic bacteria isolated from Nicotiana glauca organs

Biological Control ◽

10.1016/j.biocontrol.2016.03.005 ◽

2016 ◽

Vol 97 ◽

pp. 80-88 ◽

Cited By ~ 50

Author(s):

Rania Aydi Ben Abdallah ◽

Sonia Mokni-Tlili ◽

Ahlem Nefzi ◽

Hayfa Jabnoun-Khiareddine ◽

Mejda Daami-Remadi

Keyword(s):

Fusarium Wilt ◽

Endophytic Bacteria ◽

Growth Promotion ◽

Nicotiana Glauca ◽

Tomato Plants

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Characterization of bacterial endophytes from the roots of native and cultivated Brazil nut trees (Bertholletia excelsa)

Acta Amazonica ◽

10.1590/1809-4392201804831 ◽

2019 ◽

Vol 49 (4) ◽

pp. 257-267

Author(s):

Patrícia Bombonati CHALITA ◽

Eliane do Nascimento Cunha FARIAS ◽

Ismaele Breckenfeld da COSTA ◽

Brenda Ferreira SOUSA ◽

Marco Antônio Oliveira dos SANTOS ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Endophytic Bacteria ◽

Growth Promotion ◽

Nifh Gene ◽

Aluminum Phosphate ◽

Brazil Nut ◽

Indole Compounds ◽

Plant Growth Promoting ◽

Growth Promoting

ABSTRACT Brazil nut is a very important nontimber forest product in the Amazon region. Propagation of this tree still represents a challenge due to slow and uneven seed germination. In this context, plant growth-promoting bacteria can facilitate the process of propagation. The aims of this study were to isolate and characterize endophytic bacteria from the roots of Brazil nut trees in native terra firme forest and cultivation areas in northern Brazil, and to identify mechanisms by which bacteria act in plant growth promotion. Overall, 90 bacterial isolates were obtained from the roots of Brazil nut trees in monoculture, agroforestry and native forest areas by using different semisolid media. The isolates were characterized by sequencing the 16S rRNA gene. Plant growth-promoting characteristics were evaluated by the presence of the nifH gene, aluminum phosphate solubilization and the production of indole compounds. The isolates were affiliated with 18 genera belonging to 5 different classes (α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, Bacilli and Actinobacteria). The genus Bacillus was predominant in the forest and monoculture areas. Fourteen isolates presented the nifH gene. Most of the bacteria were able to solubilize aluminum phosphate and synthetize indole compounds. The results indicated high diversity of endophytic bacteria present among the roots of Brazil nut trees, mainly in the agroforestry area, which could be related to soil attributes. Among the 90 isolates, the 22 that presented the best results regarding plant growth promotion traits were good candidates for testing in seedling production of Brazil nut trees.

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