scholarly journals Comparison and Evaluation of Mitotic Figures in Oral Epithelial Dysplasia using Crystal Violet and Feulgen Stain

2014 ◽  
Vol 15 (3) ◽  
pp. 273-277 ◽  
Author(s):  
Shankargouda Patil ◽  
Anveeta Agarwal
Keyword(s):  
Crystal Violet ◽  
Apoptotic Cell ◽  
Cost Effective ◽  
Oral Pathology ◽  
Mitotic Cell ◽  
Epithelial Dysplasia ◽  
Oral Epithelial Dysplasia ◽  
Tissue Sections ◽  
Mitotic Figures ◽  
Oral Epithelial

ABSTRACT Background Routine staining procedures often pose a problem in differentiating a mitotic cell from an apoptotic cell, deteriorating the reliability of histology grading. Although various new methods have been recommended for identifying mitotic figures (MFs) in tissues, the time factor and cost makes them less feasible. Thus, an attempt was made to evaluate the efficacy of crystal violet and Feulgen reaction in identifying MFs and also to see for any variation in the number of MFs in various grades of Epithelial dysplasia. Objectives: 1. Using crystal violet and Feulgen stain in the identification and counting of MFs on diagnosed cases of epithelial dysplasia and thereby to evaluate their efficacy. 2. To evaluate the variation in the number of MFs in various grades of epithelial dysplasia. Materials and methods The study sample includes retrieval of 30 formalin fixed paraffin embedded tissue sections diagnosed for different grades of epithelial dysplasia (WHO grading system, 2005) from the archives, Department of Oral Pathology, MSRDC, Bengaluru. Ten tissue sections each of mild, moderate and severe epithelial dysplasia were stained with H&E, Feulgen and 1% crystal violet stains and the number of MFs were counted. Five cases of cervical carcinoma were taken as control. Stained sections were compared, and data obtained was statistically analyzed using the Kruskal-Wallis test. Results A significant increase in the number of MFs (p = 0.02) was observed in Feulgen stained sections as compared to H&E stain. Conclusion Feulgen stain can be considered as a simple, reliable, cost-effective and reproducible method of staining MFs. How to cite this article Rao RS, Patil S, Agarwal A. Comparison and Evaluation of Mitotic Figures in Oral Epithelial Dysplasia using Crystal Violet and Feulgen Stain. J Contemp Dent Pract 2014;15(3):273-277.

scholarly journals Efficiency of Crystal Violet Stain to Study Mitotic Figures in Oral Epithelial Dysplasia

2019 ◽  
Vol 7 (10) ◽  
pp. 1576-1581
Author(s):  
Aneequa Sajjad ◽  
Syeda Zaira Sajjad ◽  
Sadia Minhas ◽  
Muhammad Kashif ◽  
Afra Samad ◽  
...  
Keyword(s):  
Crystal Violet ◽  
Maxillofacial Surgery ◽  
Optical Microscope ◽  
Epithelial Dysplasia ◽  
Medical Institute ◽  
Mitotic Figure ◽  
Oral Epithelial Dysplasia ◽  
Significant Difference ◽  
Mitotic Figures ◽  
Oral Epithelial

AIM: To evaluate mitotic activity in the different grades of oral epithelial dysplasia using 1% crystal violet stain. MATERIAL AND METHODS: A descriptive study was conducted in the Department of Histopathology of the Post Graduate Medical Institute, Lahore on a total of thirty-three cases of the Oral Epithelial Dysplasia (OED). Fresh, frozen paraffin-embedded archival tissue blocks were collected from Lahore General Hospital, Lahore & Oral & Maxillofacial Surgery Department of Nawaz Sharif Hospital, Yakki Gate, Lahore. The representative sections were taken and, after processing, mounted on glass slides and stained with H&E and crystal violet stains. The stained slides were then examined under an optical microscope. The efficacy of 1% crystal violet stain to identify mitotic figures in the different grades of oral epithelial dysplasia was assessed with the sample t-test. A difference of p < 0.05 was considered to be significant. RESULTS: A comparison of the mitotic figure count in two categories in sections stained with both stains showed a statistically significant difference. An increase in the mean mitotic count was noted in the sections of OED stained with crystal violet in comparison to the sections of OED stained with H&E which was statistically significant (p = 0.00). CONCLUSION: Counting of mitotic cell is the rapid and simplest way of evaluating the proliferative activity of cells. Crystal violet stain can be a rationalised step in the staining of mitotic figures compared to the usual H&E staining and can be employed as a selective stain during routine histopathological procedures.

scholarly journals Efficacy of Various Stains to Study Mitotic Figures in Oral Epithelial Dysplasia - A Pilot Study

2013 ◽  
Vol 2 (2) ◽  
pp. 46 ◽  
Author(s):  
SangeetaJ Palaskar ◽  
Swati Patil ◽  
Bindiya Narang ◽  
Prashant Prabhu ◽  
Pargatsingh Kathuriya ◽  
...  
Keyword(s):  
Pilot Study ◽  
Epithelial Dysplasia ◽  
Oral Epithelial Dysplasia ◽  
Mitotic Figures ◽  
Oral Epithelial

scholarly journals USE OF CRYSTAL VIOLET STAIN FOR THE ASSESSMENT OF MITOTIC FIGURES IN ORAL EPITHELIAL DYSPLASIA AND ORAL SQUAMOUS CELL CARCINOMA

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Dr. Bipin Gandhi
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Crystal Violet ◽  
Control Group ◽  
Case Group ◽  
Apoptotic Bodies ◽  
Oral Epithelial Dysplasia ◽  
Mitotic Figures ◽  
Oral Epithelial

INTRODUCTION: Histopathological grading depends on several features, of which appearance of mitotic figures and apoptotic bodies in the tissue sections plays an important role. The mitotic figures and apoptotic bodies help assess the cellular proliferation and the turnover of the tumor. As mitotic figures (MF) and apoptotic bodies are difficult to assess under routine H and E sections, other methods such as immunohistochemistry (IHC) and flow cytometry have been implemented but, these methods are expensive and more time-consuming and are highly technique sensitive. Hence, stains, which should be cheap, and can be used routinely, and can be tried in identifying the mitotic figures and apoptotic bodies, which has come out with a better result. Routine histological procedures cannot differentiate between pyknotic nuclei, apoptotic cells and MFs which makes the diagnosis difficult. MATERIAL AND METHODS: Study samples were divided in to 3 groups, group 1- case group in which 40 samples of paraffin blocks diagnosed as oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC) and group 2- control group in which 40 samples from the healthy individuals. 5 micron thickness sections were made from each tissue specimen. One section was stained with H & E stain nd another section was stained with 1% crystal violet stain. RESULTS: In case group there were 24(60%) male and 16(40%) female while in control group there were 22 (55%) male and 18(45%) female. Mean age in case group was 36.2±15.4 and in control group was25.7±12.7. There is a significant increase in mitotic figures of H & E (P< 0.01) and crystal violet (P < 0.01). In case, there was a significant (P<0.05) increase in mitotic figures when stained with crystal violet. As compared to H&E stain there was a significant (P < 0.01) increase in metaphase observed in crystal violet stain. Mean mitotic figure by H&E in case group was 4.15±1.07 while in crystal violet it was 5.97±1.87. CONCLUSION: % crystal violet stain is the cheaper stain and can be performed in small laboratories and resource constraint settings. Crystal violet can be used for the localization of mitotic figures and assessing proliferation.

Computational analysis of histological images from hematoxylin and eosin-stained oral epithelial dysplasia tissue sections

2022 ◽  
pp. 116456
Author(s):  
Adriano Barbosa Silva ◽  
Alessandro Santana Martins ◽  
Thaína Aparecida Azevedo Tosta ◽  
Leandro Alves Neves ◽  
João Paulo Silva Servato ◽  
...  
Keyword(s):  
Computational Analysis ◽  
Epithelial Dysplasia ◽  
Oral Epithelial Dysplasia ◽  
Tissue Sections ◽  
Histological Images ◽  
Hematoxylin And Eosin ◽  
Oral Epithelial

scholarly journals SAVER: sodium valproate for the epigenetic reprogramming of high-risk oral epithelial dysplasia—a phase II randomised control trial study protocol

Trials ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Caroline McCarthy ◽  
Joseph Sacco ◽  
Stefano Fedele ◽  
Michael Ho ◽  
Stephen Porter ◽  
...  
Keyword(s):  
High Risk ◽  
Sodium Valproate ◽  
Study Protocol ◽  
Epigenetic Reprogramming ◽  
Epithelial Dysplasia ◽  
Phase Iii ◽  
Cancer Development ◽  
Randomised Control Trial ◽  
Oral Epithelial Dysplasia ◽  
Oral Epithelial

Abstract Background Sodium valproate (VPA) has been associated with a reduced risk of head and neck cancer development. The potential protective mechanism of action is believed to be via inhibition of histone deacetylase and subsequent epigenetic reprogramming. SAVER is a phase IIb open-label, randomised control trial of VPA as a chemopreventive agent in patients with high-risk oral epithelial dysplasia (OED). The aim of the trial is to gather preliminary evidence of the clinical and biological effects of VPA upon OED and assess the feasibility and acceptability of such a trial, with a view to inform a future definitive phase III study. Methods One hundred and ten patients with high-risk OED will be recruited from up to 10 secondary care sites in the UK and randomised into either VPA or observation only for 4 months. Women of childbearing potential will be excluded due to the teratogenic properties of VPA. Tissue and blood samples will be collected prior to randomisation and on the last day of the intervention/observation-only period (end of 4 months). Clinical measurement and additional safety bloods will be taken at multiple time points during the trial. The primary outcome will be a composite, surrogate endpoint of change in lesion size, change in grade of dysplasia and change in LOH profile at 8 key microsatellite regions. Feasibility outcomes will include recruitment targets, compliance with the study protocol and adverse effects. A qualitative sub-study will explore patient experience and perception of the trial. Discussion The current management options for patients with high-risk OED are limited and mostly include surgical resection and clinical surveillance. However, there remains little evidence whether surgery can effectively lead to a notable reduction in the risk of oral cancer development. Similarly, surveillance is associated with concerns regarding delayed diagnosis of OED progressing to malignancy. The SAVER trial provides an opportunity to investigate the effects of a repurposed, inexpensive and well-tolerated medication as a potential chemopreventive strategy for patients with high-risk OED. The clinical and biological findings of SAVER will inform the appropriateness, design and feasibility of a definitive phase III trial. Trial registration The trial is registered with the European Clinical Trials Database (Eudra-CT 2018-000197-30). (http://www.isrctn.com/ISRCTN12448611). The trial was prospectively registered on 24/04/2018.

Observer agreement in the grading of oral epithelial dysplasia

2003 ◽  
Vol 31 (4) ◽  
pp. 300-305 ◽  
Author(s):  
Brothwell D. J ◽  
Lewis D. W ◽  
Bradley G ◽  
Leong I ◽  
Jordan R. C. K ◽  
...  
Keyword(s):  
Observer Agreement ◽  
Epithelial Dysplasia ◽  
Oral Epithelial Dysplasia ◽  
Oral Epithelial
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